LOCUS       sequence-440198-       20584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows inducible expression of gRNAs and Cas9 for plasmid curing as 
            well as self-curing via a temperature sensitive RK2 replicon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20584)
  AUTHORS   Lauritsen I, Porse A, Sommer MOA, Norholm MHH
  TITLE     A versatile one-step CRISPR-Cas9 based approach to plasmid-curing.
  JOURNAL   Microb Cell Fact. 2017 Aug 2;16(1):135. doi: 
            10.1186/s12934-017-0748-z.
  PUBMED    28764701
REFERENCE   2  (bases 1 to 20584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-08-2"; volume: "16(1):135. doi"; pages: " 
            10.1186/s12934-017-0748-z"
COMMENT     Sequence Label: pFREE-RK2
FEATURES             Location/Qualifiers
     source          1..20584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1276..1807
                     /label=oriV
                     /note="incP origin of replication"
     CDS             2536..3681
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDLVHCKR"
     misc_feature    3721..3739
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_RNA        complement(3960..4038)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     promoter        complement(4039..4073)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     repeat_region   4210..4245
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4276..4311
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4342..4377
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4408..4443
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4474..4509
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     protein_bind    4583..4601
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4608..4626
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             4712..6430
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     terminator      8866..8913
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             9047..9670
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             complement(9832..10647)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9832..10647)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(10648..10739)
                     /gene="bla"
                     /label=AmpR promoter
     RBS             10822..10830
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    10845..10863
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     rep_origin      10899..11430
                     /label=oriV
                     /note="incP origin of replication"
     CDS             12159..13304
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDLVHCKR"
     misc_feature    13344..13362
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_RNA        complement(13583..13661)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     promoter        complement(13662..13696)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     repeat_region   13833..13868
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   13899..13934
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   13965..14000
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   14031..14066
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   14097..14132
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     protein_bind    14206..14224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    14231..14249
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             14335..16053
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     terminator      18489..18536
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             18670..19293
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             complement(19455..20270)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(19455..20270)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(20271..20362)
                     /gene="bla"
                     /label=AmpR promoter
     RBS             20445..20453
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    20468..20486
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 acgagtaagt cttcccttgt tattgtgtag ccagaatgcc gcaaaacttc catgcctaag
       61 cgaactgttg agagtacgtt tcgatttctg actgtgttag cctggaagtg cttgtcccaa
      121 ccttgtttct gagcatgaac gcccgcaagc caacatgtta gttgaagcat cagggcgatt
      181 agcagcatga tatcaaaacg ctctgagctg ctcgttcggc tatggcgtag gcctagtccg
      241 taggcaggac ttttcaagtc tcggaaggtt tcttcaatct gcattcgctt cgaatagata
      301 ttaacaagtt gtttgggtgt tcgaatttca acaggtaagt tagttgctag aacccatggc
      361 tcctttgccg acgctgagta gattttaggt gacgggtggt gacaatgagt ccgtgtcgag
      421 cgctgatttt ttcggccttt agagcgagat ttatacaata gaatttggca tgagattgga
      481 ttgcttttag tcagcctctt atagcctaaa gtctttgagt gactagatga catatcatgt
      541 aagttgctga taggtttcca gttttccgct cctaggtctg catattgtac ttttcctctt
      601 actcgactta accagtacca acccagcttc tcaacggatt tataccatgg cactttaaag
      661 ccagcatcac tgacaatgag cggtgtggtg ttactcggta gaatgctcgc aaggtcggct
      721 agaaattggt catgagcttt ctttgaacat tgctctgaaa gcgggaacgc tttctcataa
      781 agagtaacag aacgaccgtg tagtgcgact gaagctcgca ataccataag tcgtttttgc
      841 tcacgaatat cagaccagtc aacaagtaca atgggcatcg tattgcccga acagataaag
      901 ctagcatgcc aacggtatac agcgagtcgc tctttgtgga ggtgacgatt acctaacaat
      961 cggtcgattc gtttgatgtt atgttttgtt ctcgctttgg ttggcaggtt acggccaagt
     1021 tcggtaagag tgagagtttt acagtcaagt aatgcgtggc aagccaacgt taagctgttg
     1081 agtcgtttta agtgtaattc ggggcagaat tggtaaagag agtcgtgtaa aatatcgagt
     1141 tcgcacatct tgttgtctga ttattgattt ttcgcgaaac catttgatca tatgacaaga
     1201 tgtgtatcca ccttaactta atgattttta ccaaaatcat taggggattc atcagagccg
     1261 tgtgcgagac accgcggccg ccggcgttgt ggatacctcg cggaaaactt ggccctcact
     1321 gacagatgag gggcggacgt tgacacttga ggggccgact cacccggcgc ggcgttgaca
     1381 gatgaggggc aggctcgatt tcggccggcg acgtggagct ggccagcctc gcaaatcggc
     1441 gaaaacgcct gattttacgc gagtttccca cagatgatgt ggacaagcct ggggataagt
     1501 gccctgcggt attgacactt gaggggcgcg actactgaca gatgaggggc gcgatccttg
     1561 acacttgagg ggcagagtgc tgacagatga ggggcgcacc tattgacatt tgaggggctg
     1621 tccacaggca gaaaatccag catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa
     1681 cctgtctttt aacctgcttt taaaccaata tttataaacc ttgtttttaa ccagggctgc
     1741 gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg aaccctcccg
     1801 gcccgctaac gcgggcctcc catcccccca ggggctgcgc ccctcggccg cgaacggcct
     1861 caccccaaaa atggcagcgc tggcagtcct tgccattgcc gggatcgggg cagtaacggg
     1921 atgggcgatc agcccgagcg cgacgcccgg aagcattgac gtgccgcagg tgctggcatc
     1981 gacattcagc gaccaggtgc cgggcagtga gggcggcggc ctgggtggcg gcctgccctt
     2041 cacttcggcc gtcggggcat tcacggactt catggcgggg ccggcaattt ttaccttggg
     2101 cattcttggc atagtggtcg cgggtgccgt gctcgtgttc gggggtgaat taattccccg
     2161 gatccgtcag cttcacgctg ccgcaagcac tcagggcgca agggctgcta aaggaagcgg
     2221 aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg
     2281 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt
     2341 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct
     2401 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg
     2461 ccaaggatct gatggcgcag gggatcaaga tcgacggatc cggggaattc cggggcaatc
     2521 ccgcaaggag ggtgaatgaa tcggacgttt gaccggaagg catacaggca agaactgatc
     2581 gacgcggggt tttccgccga ggatgccgaa accatcgcaa gccgcaccgt catgcgtgcg
     2641 ccccgcgaaa ccttccagtc cgtcggctcg atggtccagc aagctacggc caagatcgag
     2701 cgcgacagcg tgcaactggc tccccctgcc ctgcccgcgc catcggccgc cgtggagcgt
     2761 tcgcgtcgtc tcgaacagga ggcggcaggt ttggcgaagt cgatgaccat cgacacgcga
     2821 ggaactatga cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa acaggtcagc
     2881 gaggccaagc aggccgcgtt gctgaaacac acgaagcagc agatcaagga aatgcagctt
     2941 tccttgttcg atattgcgcc gtggccggac acgatgcgag cgatgccaaa cgacacggcc
     3001 cgctctgccc tgttcaccac gcgcaacaag aaaatcccgc gcgaggcgct gcaaaacaag
     3061 gtcattttcc acgtcaacaa ggacgtgaag atcacctaca ccggcgtcga gctgcgggcc
     3121 gacgatgacg aactggtgtg gcagcaggtg ttggagtacg cgaagcgcac ccctatcggc
     3181 gagccgatca ccttcacgtt ctacgagctt tgccaggacc tgggctggtc gatcaatggc
     3241 cggtattaca cgaaggccga ggaatgcctg tcgcgcctac aggcgacggc gatgggcttc
     3301 acgtccgacc gcgttgggca cctggaatcg gtgtcgctgc tgcaccgctt ccgcgtcctg
     3361 gaccgtggca agaaaacgtc ccgttgccag gtcctgatcg acgaggaaat cgtcgtgctg
     3421 tttgctggcg accactacac gaaattcata tgggagaagt accgcaagct gtcgccgacg
     3481 gcccgacgga tgttcgacta tttcagctcg caccgggagc cgtacccgct caagctggaa
     3541 accttccgcc tcatgtgcgg atcggattcc acccgcgtga agaagtggcg cgagcaggtc
     3601 ggcgaagcct gcgaagagtt gcgaggcagc ggcctggtgg aacacgcctg ggtcaatgac
     3661 ctggtgcatt gcaaacgcta gggccttgtg gggtcagttc cggctggggg ttcagcacag
     3721 ctgtctctta tacacatctc ttcaaatgta gcacctgaag tcagccccat acgatataag
     3781 ttgtaattct catgttagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
     3841 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg
     3901 cgttgcgcac accgactagc gaaaaaaccc cgccgaagcg gggttttttg cgaaaaaaag
     3961 caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat
     4021 gctgttttga atggttccgc tagcactgta cctaggactg agctagccgt caaaagcagc
     4081 gtatatacca tggccacaat tcagcaaatt gtgaacatca tcacgttcat ctttccctgg
     4141 ttgccaatgg cccattttcc tgtcagtaac gagaaggtcg cgaattcagg cgctttttag
     4201 actggtcgtg ttttagagct atgctgtttt gaatggtccc aaaacatgaa ctagcgatta
     4261 gtcgctatga cttaagtttt agagctatgc tgttttgaat ggtcccaaaa caaccacact
     4321 agagaacata ctggctaaat agttttagag ctatgctgtt ttgaatggtc ccaaaacggt
     4381 tggactcaag acgatagtta ccggatagtt ttagagctat gctgttttga atggtcccaa
     4441 aacggcgaaa cccgacagga ctataaagat accgttttag agctatgctg ttttgaatgg
     4501 tcccaaaaca aaaaaaaacc ccgcccctga cagggcgggg tttttttttt aattaaagtc
     4561 tggtcctcga gtctggttat aatccctatc agtgatagag attgacatcc ctatcagtga
     4621 tagagatact gagcacagaa ggagatatac atggataaga aatactgagc actatcagtg
     4681 atagagatac tgagcacaga aggagatata catggataag aaatactcaa taggcttaga
     4741 tatcggcaca aatagcgtcg gatgggcggt gatcactgat gaatataagg ttccgtctaa
     4801 aaagttcaag gttctgggaa atacagaccg ccacagtatc aaaaaaaatc ttataggggc
     4861 tcttttattt gacagtggag agacagcgga agcgactcgt ctcaaacgga cagctcgtag
     4921 aaggtataca cgtcggaaga atcgtatttg ttatctacag gagatttttt caaatgagat
     4981 ggcgaaagta gatgatagtt tctttcatcg acttgaagag tcttttttgg tggaagaaga
     5041 caagaagcat gaacgtcatc ctatttttgg aaatatagta gatgaagttg cttatcatga
     5101 gaaatatcca actatctatc atctgcgaaa aaaattggta gattctactg ataaagcgga
     5161 tttgcgctta atctatttgg ccttagcgca tatgattaag tttcgtggtc attttttgat
     5221 tgagggagat ttaaatcctg ataatagtga tgtggacaaa ctatttatcc agttggtaca
     5281 aacctacaat caattatttg aagaaaaccc tattaacgca agtggagtag atgctaaagc
     5341 gattctttct gcacgattga gtaaatcaag acgattagaa aatctcattg ctcagctccc
     5401 cggtgagaag aaaaatggct tatttgggaa tctcattgct ttgtcattgg gtttgacccc
     5461 taattttaaa tcaaattttg atttggcaga agatgctaaa ttacagcttt caaaagatac
     5521 ttacgatgat gatttagata atttattggc gcaaattgga gatcaatatg ctgatttgtt
     5581 tttggcagct aagaatttat cagatgctat tttactttca gatatcctaa gagtaaatac
     5641 tgaaataact aaggctcccc tatcagcttc aatgattaaa cgctacgatg aacatcatca
     5701 agacttgact cttttaaaag ctttagttcg acaacaactt ccagaaaagt ataaagaaat
     5761 cttttttgat caatcaaaaa acggatatgc aggttatatt gatgggggag ctagccaaga
     5821 agaattttat aaatttatca aaccaatttt agaaaaaatg gatggtactg aggaattatt
     5881 ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg acctttgaca acggctctat
     5941 tccccatcaa attcacttgg gtgagctgca tgctattttg agaagacaag aagactttta
     6001 tccattttta aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc gaattcctta
     6061 ttatgttggt ccattggcgc gtggcaatag tcgttttgca tggatgactc ggaagtctga
     6121 agaaacaatt accccatgga attttgaaga agttgtcgat aaaggtgctt cagctcaatc
     6181 atttattgaa cgcatgacaa actttgataa aaatcttcca aatgaaaaag tactaccaaa
     6241 acatagtttg ctttatgagt attttacggt ttataacgaa ttgacaaagg tcaaatatgt
     6301 tactgaagga atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag ccattgttga
     6361 tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag attatttcaa
     6421 aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt gaagatagat ttaatgcttc
     6481 attaggtacc taccatgatt tgctaaaaat tattaaagat aaagattttt tggataatga
     6541 agaaaatgaa gattcttaga ggatattgtt ttaacattga ccttatttga agatagggag
     6601 atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag
     6661 cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt
     6721 agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat
     6781 cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa
     6841 gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc
     6901 cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta
     6961 atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact
     7021 caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa
     7081 ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag
     7141 ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt
     7201 aatcgtttaa gtgattatga tgtcgatcac attgttccac aaagtttcct taaagacgat
     7261 tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt
     7321 ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag
     7381 ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa
     7441 cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat
     7501 gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt
     7561 cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc
     7621 caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat
     7681 gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat
     7741 ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc
     7801 aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt
     7861 acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga
     7921 gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc
     7981 caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt
     8041 ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa
     8101 tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa
     8161 aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa
     8221 agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt
     8281 aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt
     8341 aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc
     8401 aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa
     8461 gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt
     8521 gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt
     8581 cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt
     8641 catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca
     8701 attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa
     8761 tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg tgactgagat
     8821 cccatgttac cggtatccta ggctgctgcc accgctgagc aataactagc ataacccctt
     8881 ggggcctcta aacgggtctt gaggggtttt ttgctgaaac ctcaggcatt tgagaagcac
     8941 acggtcacac tgcttccggt agtcaataaa ccggtattga cgccaacggg tcacacgggt
     9001 acagtgctag cagaaacata tccatgaaat cccgccccga attcatatgt ctagattaga
     9061 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
     9121 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
     9181 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
     9241 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
     9301 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
     9361 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
     9421 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
     9481 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
     9541 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
     9601 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag
     9661 tgggtcttaa gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgacaa
     9721 gctgacgacc gggtctccgc aagtggcact tttcggggaa atgtgcgcgg aacccctatt
     9781 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgaattaatt cttagaaaaa
     9841 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
     9901 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
     9961 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
    10021 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
    10081 tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
    10141 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
    10201 gagacgaaat acgcggtcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
    10261 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
    10321 tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt
    10381 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
    10441 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg
    10501 cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg
    10561 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca
    10621 agacgtttcc cgttgaatat ggctcatact cttccttttt caatattatt gaagcattta
    10681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10741 aggcatgcta gcgcagaata aatatctaac accgtgcgtg ttgactattt tacctctggc
    10801 ggtgataatg gttgcatgta ctaaggaggt tgtatggaac aacgagatgt gtataagaga
    10861 cagctgaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc
    10921 tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg
    10981 actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga
    11041 gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc ccacagatga
    11101 tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg
    11161 acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc
    11221 acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc
    11281 gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa
    11341 accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg
    11401 cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc ccaggggctg
    11461 cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggcagt ccttgccatt
    11521 gccgggatcg gggcagtaac gggatgggcg atcagcccga gcgcgacgcc cggaagcatt
    11581 gacgtgccgc aggtgctggc atcgacattc agcgaccagg tgccgggcag tgagggcggc
    11641 ggcctgggtg gcggcctgcc cttcacttcg gccgtcgggg cattcacgga cttcatggcg
    11701 gggccggcaa tttttacctt gggcattctt ggcatagtgg tcgcgggtgc cgtgctcgtg
    11761 ttcgggggtg aattaattcc ccggatccgt cagcttcacg ctgccgcaag cactcagggc
    11821 gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac
    11881 cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa
    11941 agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag
    12001 caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag
    12061 taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca agatcgacgg
    12121 atccggggaa ttccggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga
    12181 aggcatacag gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg
    12241 caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc
    12301 agcaagctac ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg
    12361 cgccatcggc cgccgtggag cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga
    12421 agtcgatgac catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg
    12481 aggacctggc aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc
    12541 agcagatcaa ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc
    12601 gagcgatgcc aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc
    12661 cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct
    12721 acaccggcgt cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt
    12781 acgcgaagcg cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg
    12841 acctgggctg gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc
    12901 tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc
    12961 tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga
    13021 tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga
    13081 agtaccgcaa gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg
    13141 agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg
    13201 tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg
    13261 tggaacacgc ctgggtcaat gacctggtgc attgcaaacg ctagggcctt gtggggtcag
    13321 ttccggctgg gggttcagca cagctgtctc ttatacacat ctcttcaaat gtagcacctg
    13381 aagtcagccc catacgatat aagttgtaat tctcatgtta gtcatgcccc gcgcccaccg
    13441 gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg
    13501 agtgagctaa cttacattaa ttgcgttgcg cacaccgact agcgaaaaaa ccccgccgaa
    13561 gcggggtttt ttgcgaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga
    13621 ctagccttat tttaacttgc tatgctgttt tgaatggttc cgctagcact gtacctagga
    13681 ctgagctagc cgtcaaaagc agcgtatata ccatggccac aattcagcaa attgtgaaca
    13741 tcatcacgtt catctttccc tggttgccaa tggcccattt tcctgtcagt aacgagaagg
    13801 tcgcgaattc aggcgctttt tagactggtc gtgttttaga gctatgctgt tttgaatggt
    13861 cccaaaacat gaactagcga ttagtcgcta tgacttaagt tttagagcta tgctgttttg
    13921 aatggtccca aaacaaccac actagagaac atactggcta aatagtttta gagctatgct
    13981 gttttgaatg gtcccaaaac ggttggactc aagacgatag ttaccggata gttttagagc
    14041 tatgctgttt tgaatggtcc caaaacggcg aaacccgaca ggactataaa gataccgttt
    14101 tagagctatg ctgttttgaa tggtcccaaa acaaaaaaaa accccgcccc tgacagggcg
    14161 gggttttttt tttaattaaa gtctggtcct cgagtctggt tataatccct atcagtgata
    14221 gagattgaca tccctatcag tgatagagat actgagcaca gaaggagata tacatggata
    14281 agaaatactg agcactatca gtgatagaga tactgagcac agaaggagat atacatggat
    14341 aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc ggtgatcact
    14401 gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt
    14461 atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact
    14521 cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta
    14581 caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa
    14641 gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata
    14701 gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg
    14761 gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt
    14821 aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac
    14881 aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac
    14941 gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta
    15001 gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg gaatctcatt
    15061 gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct
    15121 aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt
    15181 ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt
    15241 tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt
    15301 aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa
    15361 cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat
    15421 attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa
    15481 atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa
    15541 cggacctttg acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt
    15601 ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa
    15661 atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt
    15721 gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc
    15781 gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt
    15841 ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac
    15901 gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt
    15961 gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag
    16021 caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga
    16081 gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa
    16141 gataaagatt ttttggataa tgaagaaaat gaagattctt agaggatatt gttttaacat
    16201 tgaccttatt tgaagatagg gagatgattg aggaaagact taaaacatat gctcacctct
    16261 ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg ggacgtttgt
    16321 ctcgaaaatt gattaatggt attagggata agcaatctgg caaaacaata ttagattttt
    16381 tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct gatccatgat gatagtttga
    16441 catttaaaga agacattcaa aaagcacaag tgtctggaca aggcgatagt ttacatgaac
    16501 atattgcaaa tttagctggt agccctgcta ttaaaaaagg tattttacag actgtaaaag
    16561 ttgttgatga attggtcaaa gtaatggggc ggcataagcc agaaaatatc gttattgaaa
    16621 tggcacgtga aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac
    16681 gaatcgaaga aggtatcaaa gaattaggaa gtcagattct taaagagcat cctgttgaaa
    16741 atactcaatt gcaaaatgaa aagctctatc tctattatct ccaaaatgga agagacatgt
    16801 atgtggacca agaattagat attaatcgtt taagtgatta tgatgtcgat cacattgttc
    16861 cacaaagttt ccttaaagac gattcaatag acaataaggt cttaacgcgt tctgataaaa
    16921 atcgtggtaa atcggataac gttccaagtg aagaagtagt caaaaagatg aaaaactatt
    16981 ggagacaact tctaaacgcc aagttaatca ctcaacgtaa gtttgataat ttaacgaaag
    17041 ctgaacgtgg aggtttgagt gaacttgata aagctggttt tatcaaacgc caattggttg
    17101 aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg aatactaaat
    17161 acgatgaaaa tgataaactt attcgagagg ttaaagtgat taccttaaaa tctaaattag
    17221 tttctgactt ccgaaaagat ttccaattct ataaagtacg tgagattaac aattaccatc
    17281 atgcccatga tgcgtatcta aatgccgtcg ttggaactgc tttgattaag aaatatccaa
    17341 aacttgaatc ggagtttgtc tatggtgatt ataaagttta tgatgttcgt aaaatgattg
    17401 ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac tctaatatca
    17461 tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa cgccctctaa
    17521 tcgaaactaa tggggaaact ggagaaattg tctgggataa agggcgagat tttgccacag
    17581 tgcgcaaagt attgtccatg ccccaagtca atattgtcaa gaaaacagaa gtacagacag
    17641 gcggattctc caaggagtca attttaccaa aaagaaattc ggacaagctt attgctcgta
    17701 aaaaagactg ggatccaaaa aaatatggtg gttttgatag tccaacggta gcttattcag
    17761 tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt
    17821 tactagggat cacaattatg gaaagaagtt cctttgaaaa aaatccgatt gactttttag
    17881 aagctaaagg atataaggaa gttaaaaaag acttaatcat taaactacct aaatatagtc
    17941 tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa ttacaaaaag
    18001 gaaatgagct ggctctgcca agcaaatatg tgaatttttt atatttagct agtcattatg
    18061 aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg gagcagcata
    18121 agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt gttattttag
    18181 cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac aaaccaatac
    18241 gtgaacaagc agaaaatatt attcatttat ttacgttgac gaatcttgga gctcccgctg
    18301 cttttaaata ttttgataca acaattgatc gtaaacgata tacgtctaca aaagaagttt
    18361 tagatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc attgatttga
    18421 gtcagctagg aggtgactga gatcccatgt taccggtatc ctaggctgct gccaccgctg
    18481 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
    18541 aacctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtat
    18601 tgacgccaac gggtcacacg ggtacagtgc tagcagaaac atatccatga aatcccgccc
    18661 cgaattcata tgtctagatt agataaaagt aaagtgatta acagcgcatt agagctgctt
    18721 aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag
    18781 cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt
    18841 gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat
    18901 tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca
    18961 aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta
    19021 gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg
    19081 gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa
    19141 agggaaacac ctactactga tagtatgccg ccattattac gacaagctat cgaattattt
    19201 gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta
    19261 gaaaaacaac ttaaatgtga aagtgggtct taagcaatag acataagcgg ctatttaacg
    19321 accctgccct gaaccgacga caagctgacg accgggtctc cgcaagtggc acttttcggg
    19381 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    19441 tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
    19501 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
    19561 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
    19621 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
    19681 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact
    19741 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
    19801 ttcattcgtg attgcgcctg agcgagacga aatacgcggt cgctgttaaa aggacaatta
    19861 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
    19921 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
    19981 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
    20041 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
    20101 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
    20161 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
    20221 gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat actcttcctt
    20281 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    20341 tgtatttaga aaaataaaca aataggcatg ctagcgcaga ataaatatct aacaccgtgc
    20401 gtgttgacta ttttacctct ggcggtgata atggttgcat gtactaagga ggttgtatgg
    20461 aacaacgaga tgtgtataag agacagctga aacgccgtcg aagccgtgtg ctgagagatc
    20521 ccctcataat ttccccaaag cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc
    20581 agcc
//
