LOCUS       sequence-440214-        9309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry plasmid (attL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9309)
  TITLE     Enhancing Cas12a and Cas9 mediated plant genome editing efficiency 
            and deletion size with the sbcB exonuclease
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pYPQ166-sbcB-N
FEATURES             Location/Qualifiers
     source          1..9309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      53..641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     542..561
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     795..812
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(971..998)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1090..1176)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1225..1247
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1239..1256
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1240..1256
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1272..1371
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2822..2869
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             2927..2992
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2999..3019
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3044..7144
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3044..7144
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7057..7065
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7145..7192
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    7867..7990
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8007..8026)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8008..8026)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8031..8047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8031..8047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8478..9269
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
       61 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      121 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      181 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      241 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      301 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      361 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      421 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      481 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      541 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      601 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      661 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      721 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
      781 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
      841 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
      901 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgcgagccag gaagagtttg
      961 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     1021 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     1081 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     1141 ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
     1201 cgttaacgct tgcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta
     1261 agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
     1321 ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagcaggc tggtacgttc
     1381 cctaccggcc gcacatatga tgaatgatgg aaagcagcaa tcgacgttcc ttttccatga
     1441 ttatgaaacg ttcggaactc atcctgctct tgatagacct gctcaatttg cagctattag
     1501 aactgattct gaatttaatg ttattggaga acctgaagtt ttttattgta agcctgctga
     1561 tgattatctt ccacaacctg gagctgttct tattactgga attactcctc aagaagctag
     1621 agctaaggga gagaatgaag cagctttcgc agctagaatt cattctctat ttactgttcc
     1681 taagacttgt attcttggat ataacaatgt tagattcgac gatgaagtta ctagaaatat
     1741 tttttatagg aatttctatg atccttatgc ttggtcttgg caacatgaca attctagatg
     1801 ggatctactt gatgttatga gagcttgtta tgctcttaga cctgaaggaa ttaattggcc
     1861 tgaaaatgac gatggacttc cttctttcag acttgaacat cttactaagg ctaatggaat
     1921 tgaacattct aatgctcatg atgcaatggc tgatgtttat gcaactattg caatggctaa
     1981 gcttgttaag actagacaac ctagactttt cgattatctt tttactcata gaaataagca
     2041 taagcttatg gctcttattg atgttcctca aatgaagcct cttgttcatg tttctggaat
     2101 gttcggagct tggagaggaa atacttcttg ggttgcacct cttgcttggc atcctgaaaa
     2161 tagaaacgct gttattatgg ttgatcttgc tggagatatt tctcctctgc ttgaactgga
     2221 ttctgatact cttagagaaa gactttatac tgctaagact gatcttggag ataatgccgc
     2281 tgttcctgtg aagcttgttc atattaataa gtgtcctgtt cttgctcaag cgaatactct
     2341 tagacctgaa gacgctgata gacttggaat taatagacaa cattgtcttg ataatctgaa
     2401 gattcttaga gaaaatcctc aagttagaga aaaggtcgtt gctattttcg ctgaagcgga
     2461 accttttact ccttctgata atgttgatgc tcaactgtat aatggattct tttctgatgc
     2521 tgacagagct gcaatgaaga ttgttcttga gactgaacct agaaatctgc ctgctcttga
     2581 tattacgttc gttgataaga gaatcgaaaa gctgcttttc aattatagag ctaggaattt
     2641 tcctggaact cttgattatg ctgaacaaca aagatggctt gaacatagga gacaagtttt
     2701 cactcctgaa tttcttcaag gatatgctga tgaacttcaa atgcttgttc agcaatatgc
     2761 tgatgacaag gaaaaggttg ctctccttaa ggctctttgg caatatgctg aagagatttt
     2821 taaacgtcct gctgccacca aaaaggccgg acaggctaag aaaaagaagg agctctcagg
     2881 atctgagacc ccaggcacaa gtgaatcagc tacacctgag tccatggatt acaaggacca
     2941 cgacggggat tacaaggacc acgacattga ttacaaggat gatgatgaca agatggctcc
     3001 gaagaagaag aggaaggttg gcatccacgg ggtgccagct gctgacaaga agtactcgat
     3061 cggcctcgat attgggacta actctgttgg ctgggccgtg atcaccgacg agtacaaggt
     3121 gccctcaaag aagttcaagg tcctgggcaa caccgatcgg cattccatca agaagaatct
     3181 cattggcgct ctcctgttcg acagcggcga gacggctgag gctacgcggc tcaagcgcac
     3241 cgcccgcagg cggtacacgc gcaggaagaa tcgcatctgc tacctgcagg agattttctc
     3301 caacgagatg gcgaaggttg acgattcttt cttccacagg ctggaggagt cattcctcgt
     3361 ggaggaggat aagaagcacg agcggcatcc aatcttcggc aacattgtcg acgaggttgc
     3421 ctaccacgag aagtacccta cgatctacca tctgcggaag aagctcgtgg actccacaga
     3481 taaggcggac ctccgcctga tctacctcgc tctggcccac atgattaagt tcaggggcca
     3541 tttcctgatc gagggggatc tcaacccgga caatagcgat gttgacaagc tgttcatcca
     3601 gctcgtgcag acgtacaacc agctcttcga ggagaacccc attaatgcgt caggcgtcga
     3661 cgcgaaggct atcctgtccg ctaggctctc gaagtctcgg cgcctcgaga acctgatcgc
     3721 ccagctgccg ggcgagaaga agaacggcct gttcgggaat ctcattgcgc tcagcctggg
     3781 gctcacgccc aacttcaagt cgaatttcga tctcgctgag gacgccaagc tgcagctctc
     3841 caaggacaca tacgacgatg acctggataa cctcctggcc cagatcggcg atcagtacgc
     3901 ggacctgttc ctcgctgcca agaatctgtc ggacgccatc ctcctgtctg atattctcag
     3961 ggtgaacacc gagattacga aggctccgct ctcagcctcc atgatcaagc gctacgacga
     4021 gcaccatcag gatctgaccc tcctgaaggc gctggtcagg cagcagctcc ccgagaagta
     4081 caaggagatc ttcttcgatc agtcgaagaa cggctacgct gggtacattg acggcggggc
     4141 ctctcaggag gagttctaca agttcatcaa gccgattctg gagaagatgg acggcacgga
     4201 ggagctgctg gtgaagctca atcgcgagga cctcctgagg aagcagcgga cattcgataa
     4261 cggcagcatc ccacaccaga ttcatctcgg ggagctgcac gctatcctga ggaggcagga
     4321 ggacttctac cctttcctca aggataaccg cgagaagatc gagaagattc tgactttcag
     4381 gatcccgtac tacgtcggcc cactcgctag gggcaactcc cgcttcgctt ggatgacccg
     4441 caagtcagag gagacgatca cgccgtggaa cttcgaggag gtggtcgaca agggcgctag
     4501 cgctcagtcg ttcatcgaga ggatgacgaa tttcgacaag aacctgccaa atgagaaggt
     4561 gctccctaag cactcgctcc tgtacgagta cttcacagtc tacaacgagc tgactaaggt
     4621 gaagtatgtg accgagggca tgaggaagcc ggctttcctg tctggggagc agaagaaggc
     4681 catcgtggac ctcctgttca agaccaaccg gaaggtcacg gttaagcagc tcaaggagga
     4741 ctacttcaag aagattgagt gcttcgattc ggtcgagatc tctggcgttg aggaccgctt
     4801 caacgcctcc ctggggacct accacgatct cctgaagatc attaaggata aggacttcct
     4861 ggacaacgag gagaatgagg atatcctcga ggacattgtg ctgacactca ctctgttcga
     4921 ggaccgggag atgatcgagg agcgcctgaa gacttacgcc catctcttcg atgacaaggt
     4981 catgaagcag ctcaagagga ggaggtacac cggctggggg aggctgagca ggaagctcat
     5041 caacggcatt cgggacaagc agtccgggaa gacgatcctc gacttcctga agagcgatgg
     5101 cttcgcgaac cgcaatttca tgcagctgat tcacgatgac agcctcacat tcaaggagga
     5161 tatccagaag gctcaggtga gcggccaggg ggactcgctg cacgagcata tcgcgaacct
     5221 cgctggctcg ccagctatca agaaggggat tctgcagacc gtgaaggttg tggacgagct
     5281 ggtgaaggtc atgggcaggc acaagcctga gaacatcgtc attgagatgg cccgggagaa
     5341 tcagaccacg cagaagggcc agaagaactc acgcgagagg atgaagagga tcgaggaggg
     5401 cattaaggag ctggggtccc agatcctcaa ggagcacccg gtggagaaca cgcagctgca
     5461 gaatgagaag ctctacctgt actacctcca gaatggccgc gatatgtatg tggaccagga
     5521 gctggatatt aacaggctca gcgattacga cgtcgatcat atcgttccac agtcattcct
     5581 gaaggatgac tccattgaca acaaggtcct caccaggtcg gacaagaacc ggggcaagtc
     5641 tgataatgtt ccttcagagg aggtcgttaa gaagatgaag aactactggc gccagctcct
     5701 gaatgccaag ctgatcacgc agcggaagtt cgataacctc acaaaggctg agaggggcgg
     5761 gctctctgag ctggacaagg cgggcttcat caagaggcag ctggtcgaga cacggcagat
     5821 cactaagcac gttgcgcaga ttctcgactc acggatgaac actaagtacg atgagaatga
     5881 caagctgatc cgcgaggtga aggtcatcac cctgaagtca aagctcgtct ccgacttcag
     5941 gaaggatttc cagttctaca aggttcggga gatcaacaat taccaccatg cccatgacgc
     6001 gtacctgaac gcggtggtcg gcacagctct gatcaagaag tacccaaagc tcgagagcga
     6061 gttcgtgtac ggggactaca aggtttacga tgtgaggaag atgatcgcca agtcggagca
     6121 ggagattggc aaggctaccg ccaagtactt cttctactct aacattatga atttcttcaa
     6181 gacagagatc actctggcca atggcgagat ccggaagcgc cccctcatcg agacgaacgg
     6241 cgagacgggg gagatcgtgt gggacaaggg cagggatttc gcgaccgtca ggaaggttct
     6301 ctccatgcca caagtgaata tcgtcaagaa gacagaggtc cagactggcg ggttctctaa
     6361 ggagtcaatt ctgcctaagc ggaacagcga caagctcatc gcccgcaaga aggactggga
     6421 tccgaagaag tacggcgggt tcgacagccc cactgtggcc tactcggtcc tggttgtggc
     6481 gaaggttgag aagggcaagt ccaagaagct caagagcgtg aaggagctgc tggggatcac
     6541 gattatggag cgctccagct tcgagaagaa cccgatcgat ttcctggagg cgaagggcta
     6601 caaggaggtg aagaaggacc tgatcattaa gctccccaag tactcactct tcgagctgga
     6661 gaacggcagg aagcggatgc tggcttccgc tggcgagctg cagaagggga acgagctggc
     6721 tctgccgtcc aagtatgtga acttcctcta cctggcctcc cactacgaga agctcaaggg
     6781 cagccccgag gacaacgagc agaagcagct gttcgtcgag cagcacaagc attacctcga
     6841 cgagatcatt gagcagattt ccgagttctc caagcgcgtg atcctggccg acgcgaatct
     6901 ggataaggtc ctctccgcgt acaacaagca ccgcgacaag ccaatcaggg agcaggctga
     6961 gaatatcatt catctcttca ccctgacgaa cctcggcgcc cctgctgctt tcaagtactt
     7021 cgacacaact atcgatcgca agaggtacac aagcactaag gaggtcctgg acgcgaccct
     7081 catccaccag tcgattaccg gcctctacga gacgcgcatc gacctgtctc agctcggggg
     7141 cgacaagcgg ccagcggcga cgaagaaggc ggggcaggcg aagaagaaga agtgagctca
     7201 gagctttcgt tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca
     7261 ttgcgcacac accagaatcc tactgagttt gagtattatg gcattgggaa aactgttttt
     7321 cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa
     7381 ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct
     7441 caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aattataaga
     7501 gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa
     7561 gttaatatga ggagtaaaac acttgtagtt gtaccattat gcttattcac taggcaacaa
     7621 atatattttc agacctagaa aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg
     7681 ttttagacat ttatgaactt tcctttatgt aattttccag aatccttgtc agattctaat
     7741 cattgcttta taattatagt tatactcatg gatttgtagt tgagtatgaa aatatttttt
     7801 aatgcatttt atgacttgcc aattgttccg gaactctaga taagcttacc ggaaagggcg
     7861 aattcgcaac tttgtataca aaagttgaac gagaaacgta aaatgatata aatatcaata
     7921 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
     7981 agtcactatg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     8041 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     8101 atatatcatc atgcctcctc tggaccagcc aggacagaaa tgcctcgact tcgctgctac
     8161 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     8221 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     8281 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     8341 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     8401 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     8461 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     8521 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     8581 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     8641 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     8701 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     8761 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     8821 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     8881 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     8941 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     9001 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     9061 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     9121 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     9181 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     9241 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     9301 acgtgagtt
//
