LOCUS       sequence-440220-        9415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A QUAS line that expresses VSR proteins in Ae. aegypti when crossed 
            with a driver line, Opie-2 dsRed. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9415)
  TITLE     QUAS line for production of VSR (CrPV 1A, NoV-B2, and FHV- B2) 
            expressing proteins in Aedes aegypti.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 0A-1169
FEATURES             Location/Qualifiers
     source          1..9415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3013..3075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        4431..4669
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     4616..4637
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             5157..5210
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5622..5675
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(6688..6721)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    6727..6848
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6738..6757)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6792..6811
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(6969..7643,7644..7646,7647..7649))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(7031..7054)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(7229..7248)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7446..7466
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     protein_bind    complement(8203..8236)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   8893..8927
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9108..9124)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9108..9124)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9121..9143)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9132..9148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9156..9162,9163..9180,9181..9186))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9201..9222
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9339..9356)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     3241 aaaactttta aacattctct cttttacaaa aataaactta ttttgtactt taaaaacagt
     3301 catgttgtat tataaaataa gtaattagct taacttatac ataatagaaa caaattatac
     3361 ttattagtca gtcagaaaca actttggcac atatcaatat tatgctctcg acaaataact
     3421 tttttgcatt ttttgcacga tgcatttgcc tttcgcctta ttttagaggg gcagtaagta
     3481 cagtaagtac gttttttcat tactggctct tcagtactgt catctgatgt accaggcact
     3541 tcatttggca aaatattaga gatattatcg cgcaaatatc tcttcaaagt aggagcttct
     3601 aaacgcttac gcataaacga tgacgtcagg ctcatgtaaa ggtttctcat aaattttttg
     3661 cgactttgaa ccttttctcc cttgctactg acattatggc tgtatataat aaaagaattt
     3721 atgcaggcaa tgtttatcat tccgtacaat aatgccatag gccacctatt cgtcttccta
     3781 ctgcaggtca tcacagaaca catttggtct agcgtgtcca ctccgccttt agtttgatta
     3841 taatacataa ccatttgcgg tttaccggta ctttcgttga tagaagcatc ctcatcacaa
     3901 gatgataata agtataccat cttagctggc ttcggtttat atgagacgag agtaaggggt
     3961 ccgtcaaaac aaaacatcga tgttcccact ggcctggagc gactgttttt cagtacttcc
     4021 ggtatctcgc gtttgtttga tcgcacggtt cccacaatgc tagctcacgt gtcactgggt
     4081 cagtgggtaa tcgcttatcc tcggataaac aattatcctc acgggtaatc gcttatccgc
     4141 tcgggtaatc gcttatcctc gggtaatcgc ttatccttgg gtaatcgctt atcctcggat
     4201 aaacaattat cctcacgggt aatcgcttat ccgctcgggt aatcgcttat cctcgggtaa
     4261 tcgcttatcc ttgggtaatc gcttatcctc ggataaacaa ttatcctcac gggtaatcgc
     4321 ttatccgctc gggtaatcgc ttatcctcgg gtaatcgctt atcctttcac gttgggactc
     4381 agtgaggagg acctgaattc ctgcagcccg agcggagact ctagcgagcg ccggagtata
     4441 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
     4501 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta
     4561 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac
     4621 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagggaatt
     4681 gggaattcgt taacagatcg ggggatccgc ggatgtcttt tcaacaaaca aacaacaacg
     4741 caaccaacaa catcaactcc cttgaggagc ttgctgctca agaactaata gcagcacaat
     4801 ttgaaggaaa tcttgatggt ttcttttgca ctttttatgt gcagtccaaa ccacaactat
     4861 tggacttaga gagtgaatgt tattgtatgg atgattttga ttgtgggtgt gataggatca
     4921 agagagaaga agaattacgt aaactgattt tcttaacatc ggacgtttat ggatataact
     4981 ttgaagagtg gaaaggatta gtttggaaat ttgttcaaaa ttattgccca gaacatcgat
     5041 atggatcaac ttttggtaat ggattattaa ttgtgagtcc ccgtttcttt atggatcatc
     5101 ttgactggtt tcagcaatgg aaacttgttt caagtaatga tgaatgcaga gccttcgagg
     5161 gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct atgacaaaca
     5221 tgtcatgcgc ttacgagcta atcaagtcac ttccagccaa gctggagcag ctggctcagg
     5281 agacgcaagc aacgatccaa acgctcatga tcgcggatcc caacgtcaac aaggatctgc
     5341 gagcgttctg cgagttcctg accgtgcagc accagcgggc gtatcgagcg acgaacagcc
     5401 tgctcatcaa accgcgagtc gcagcagcgc ttcgcgggga ggagctggac ctgggcgagg
     5461 cggacgtcgc cgcccgggtc cgccagctaa aacaacagct ggcggagctc gagatggaaa
     5521 tcaagccagg gcaccaacaa gtggcccaag taagcggcag gcggaaggcc gcagccgcag
     5581 ctcccgtggc ccagctgggt cgcgtgggcg tggtaaatga ggagggcaga ggaagtcttc
     5641 taacatgcgg tgacgtggag gagaatcccg gccctatgcc aagcaaactc gcgctaatcc
     5701 aggaacttcc cgaccgcatt caaacggcgg tggaagcagc catgggaatg agctaccaag
     5761 acgcaccgaa caacgtgcgc agggacctcg acaacctgca cgcttgccta aacaaggcaa
     5821 aactaacggt aagtcggatg gtaacatcac tgctggagaa acccagcgtg gtggcatacc
     5881 tagagggaaa ggcccccgag gaggcaaaac caacactcga agaacgcctc cgaaagctgg
     5941 agctcagcca cagccttcca acaaccggaa gtgacccccc acccgcaaaa ctgtagttaa
     6001 ttaactagaa tgaatcgttt ttaaaataac aaatcaattg ttttataata ttcgtacgat
     6061 tctttgatta tgtaataaaa tgtgatcatt aggaagatta cgaaaaatat aaaaaatatg
     6121 agttctgtgt gtataacaaa tgctgtaaac gccacaattg tgtttgttgc aaataaaccc
     6181 atgattattt gattaaaatt gttgttttct ttgttcatag acaatagtgt gttttgccta
     6241 aacgtgtact gcataaactc catgcgagtg tatagcgagc tagtggctaa cgcttgcccc
     6301 accaaagtag attcgtcaaa atcctcaatt tcatcaccct cctccaagtt taacatttgg
     6361 ccgtcggaat taacttctaa agatgccaca taatctaata aatgaaatag agattcaaac
     6421 gtggcgtcat cgtccgtttc gaccatttcc gaaaagaact cgggcataaa ctctatgatt
     6481 tctctggacg tggtgttgtc gaaactctca aagtacgcag tcaggaacgt gcgcgacatg
     6541 tcgtcgggaa actcgcgcgg aaacatgttg ttgtaaccga acgggtccca tagcgccaaa
     6601 accaaatctg ccagcgtcaa tagaatgagc acgatgccga caatggagct ggcttggata
     6661 gcgattcgag ttaacggccg gcgcgccata acttcgtata gcatacatta tacgaagtta
     6721 tcgcgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     6781 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     6841 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     6901 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt
     6961 cgcggccgct acaggaacag gtggtggcgg ccctcggtgc gctcgtactg ctccacgatg
     7021 gtgtagtcct cgttgtggga ggtgatgtcc agcttggagt ccacgtagta gtagccgggc
     7081 agctgcacgg gcttcttggc catgtagatg gacttgaact ccaccaggta gtggccgccg
     7141 tccttcagct tcagggcctt gtggatctcg cccttcagca cgccgtcgcg ggggtacagg
     7201 cgctcggtgg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg
     7261 aagttcacgc cgatgaactt caccttgtag atgaagcagc cgtcctgcag ggaggagtcc
     7321 tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
     7381 tcggggaagg acagcttctt gtagtcgggg atgtcggcgg ggtgcttcac gtacaccttg
     7441 gagccgtact ggaactgggg ggacaggatg tcccaggcga agggcagggg gccgcccttg
     7501 gtcaccttca gcttcacggt gttgtggccc tcgtaggggc ggccctcgcc ctcgccctcg
     7561 atctcgaact cgtggccgtt cacggtgccc tccatgcgca ccttgaagcg catgaactcc
     7621 ttgatgacgt tcttggagga gcgcaccatc tcgagcacca gagacaggtt gcggcggcgg
     7681 ttggatggcg tgggcgcgtt ggcgttgttg gaccggctca tgttgtgtcg ctgtaacaga
     7741 tgctgttcaa ctgtgtttac cagatcgttg cgggctgtat ttataggcgc gataagcggg
     7801 acgggcgcct cgtgtccggt cacgcgcatg agataacgcg cggctgatat ggaggcgcgt
     7861 cctgttccga taaggagttg cgtccggctg cggttagcaa cacaggaagc tggcgtcctg
     7921 tcacgataag acaacactcg tccggtccga taatgtgatt cgtacgtgac aggacgcgac
     7981 ccgataaggc cggcctacgt gactgccgac acgtactttt ttgcactgca aaaaggttca
     8041 atgtgtggta gtgtatttgg agcgtataca acggtgtaga ctatttatgt aaaatagtct
     8101 acgaaacgta gagtttgtac tatgtatggg cccgcgtgca aaagcgtgtt tttttgcagt
     8161 gcaaaaaagt tggtggtggg gaggccaccg agtatgagcg ctataacttc gtatagcata
     8221 cattatacga agttatccta gggatctcgg atctgacaat gttcagtgca gagactcggc
     8281 tacgcctcgt ggactttgaa gttgaccaac aatgtttatt cttacctcta atagtcctct
     8341 gtggcaaggt caagattctg ttagaagcca atgaagaacc tggttgttca ataacatttt
     8401 gttcgtctaa tatttcacta ccgcttgacg ttggctgcac ttcatgtacc tcatctataa
     8461 acgcttcttc tgtatcgctc tggacgtcat cttcacttac gtgatctgat atttcactgt
     8521 cagaatcctc accaacaagc tcgtcatcgc tttgcagaag agcagagagg atatgctcat
     8581 cgtctaaaga actacccatt ttattatata ttagtcacga tatctataac aagaaaatat
     8641 atatataata agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt
     8701 taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat
     8761 agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg
     8821 cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat
     8881 atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta aaaaagattt
     8941 gcgctttact cgacctaaac tttaaacacg tcatagaatc ttcgtttgac aaaaaccaca
     9001 ttgtggccaa gctgtgtgac gcgacgcgcg ctaaagaatg gcaaaccaag tcgcgcgagc
     9061 gtcgactcta gaggatcccc gggtaccgag ctcgaattcg taatcatgtc atagctgttt
     9121 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9181 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9241 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9301 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9361 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggt
//
