LOCUS       sequence-440254-       11110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Sox5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Bergmann T, Liu Y, Skov J, Mogus L, Lee J, Pfisterer U, Handfield 
            LF, Asenjo-Martinez A, Lisa-Vargas I, Seemann SE, Lee JTH, Patikas 
            N, Kornum BR, Denham M, Hyttel P, Witter MP, Gorodkin J, Pers TH, 
            Hemberg M, Khodosevich K, Hall VJ
  TITLE     Production of human entorhinal stellate cell-like cells by forward 
            programming shows an important role of Foxp1 in reprogramming.
  JOURNAL   Front Cell Dev Biol. 2022 Aug 15;10:976549. doi: 
            10.3389/fcell.2022.976549. eCollection 2022.
  PUBMED    36046338
REFERENCE   2  (bases 1 to 11110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Dev Biol."; date: "2022-08-15"; volume: "10:976549. doi"; pages: " 
            10.3389/fcell.2022.976549. eCollection 2022"
COMMENT     Sequence Label: p-Tet-O-Sox5-Puro
FEATURES             Location/Qualifiers
     source          1..11110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        100..479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        481..679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             697..877
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    924..1049
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1542..1775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1960..2004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2302..2419
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2476..2494,2495..2517,2518..2536,2537..2559,2560..2578,
                     2579..2601,2602..2620,2621..2643,2644..2662,2663..2685,
                     2686..2704,2705..2727,2728..2746)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2779..2817
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2814..2838
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4966..5019
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5026..5625
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5026..5045)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5522..5542
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5664..6252
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5717..5737)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6135..6146)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6255..6271)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6256..6272)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6777..6957
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6983..7000)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6989..7213
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7259..7687
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7346..7365)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7556..7577
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7696..7716)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7701..8030
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7881..8016
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7943..7962
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8078..8125
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8144..8518
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8648..8769
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8685..8704)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8739..8758
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8818..8834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8818..8834)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8831..8853)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8842..8858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8866..8872,8873..8890,8891..8896))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8911..8932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9049..9066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9220..9808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9300..9319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9979..10770,10771..10839))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10602..10621
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10840..10944)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11019..11038)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
       61 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
      121 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      181 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      301 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      481 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      541 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      601 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      661 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      721 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      781 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      841 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      901 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      961 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     1021 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1081 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1141 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1201 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1261 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1321 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1381 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     2041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2221 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     2281 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2341 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2401 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaga
     2461 tctcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2521 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2581 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     2641 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     2701 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     2761 gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt
     2821 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     2881 caccgggacc gatccagcct ccgcggcccc gaattcgcca ccatgcttac tgaccctgat
     2941 ttacctcagg agtttgaaag gatgtcttcc aagcgaccag cctctccgta tggggagaca
     3001 gatggagagg tagccatggt gacaagcaga cagaaagtgg aagaggagga gagtgagagg
     3061 ctcccagcct ttcaccttcc cttacatgaa gtcgatggca ataaagttat gtcttcatta
     3121 gctccataca actcatctac ctcacctcag aaggcggaag aaggtgggcg acagagtggc
     3181 gagtccgtgt cgagcgcagc cctggggact cccgagcggc gcaagggcag cttagcggat
     3241 gtggtggaca ccctgaagca gaggaagatg gaagagctga tcaagaatga gccagaagac
     3301 actcccagta ttgaaaagct actctccaag gactggaaag acaagctcct ggcaatggga
     3361 tcagggaact ttggagaaat aaaagggact cctgagagcc tcgctgagaa ggagcggcag
     3421 ctcatgggta tgatcaatca gctgaccagt ctacgggagc aactcctggc tgcccatgat
     3481 gagcagaaga agttggctgc atctcagata gagaaacagc gtcagcagat ggagctggcc
     3541 aagcagcaac aggagcagat cgcgaggcaa cagcagcagc ttctgcagca acaacacaaa
     3601 atcaacttgc ttcagcaaca gatccaggtt caaggtcagc tgccgccatt gatgattccc
     3661 gtgttccctc ctgaccagcg gaccctcgca gcagctgccc agcaaggatt cctccttcct
     3721 cctggtttca gctacaaggc tgggtgcagt gacccttacc ctgttcagct gatcccaacc
     3781 actatggcag ctgctgctgc agcaacacca ggcttaggcc cactccagct gcaggatgaa
     3841 gtggcccagc cactgaacct atcagctaag cccaagacct ctgatggcaa atcacctgca
     3901 tcacccacct ctcctcacat gccagctctg agaataaaca gtggggctgg ccccctcaaa
     3961 gcctctgtcc cggcagcatt agctagtcct tctgcaagag ttagcacaat aggctattta
     4021 aatgaccacg atgctgtcac caaggcaatc caagaagctc gccagatgaa agagcaactc
     4081 aggcgagagc agcaagcgtt ggacgggaag gtggccgtgg tgaacagcat aggtctcagc
     4141 aactgccgga cagaaaagga aaaaacaaca ctggagagcc tgactcagca gttggcagtt
     4201 aaacagaatg aagaagggaa atttagccat ggaatgatgg atttcaatat gagtggagat
     4261 tctgacggaa gcgctggagt ctcagagtca agaatttaca gggaatccag gggacgtggt
     4321 agcaacgagc cccacataaa gcgtccaatg aatgccttca tggtgtgggc gaaagatgaa
     4381 cggaggaaaa tccttcaggc ctttcccgac atgcacaatt ccaacatcag caagatactg
     4441 ggatctcgct ggaaagctat gaccaaccta gagaaacagc catactatga ggagcaggcc
     4501 cgcctcagca aacagcacct ggagaagtac ccggactaca agtacaagcc taggccaaag
     4561 cgcacctgtc tggtggacgg caagaaactg cgtatcgggg agtacaaggc catcatgcgg
     4621 aaccggaggc aggaaatgcg acagtacttc aacgttgggc aacaagcaca gatccccatc
     4681 gcgacggccg gagttgtata ccccagcgcc atcgccatgg caggaatgcc gtcccctcac
     4741 ctgccctccg agcactcgag cgtgtccagc agcccggagc ccgggatgcc cgtgatccag
     4801 agcacttacg gcgccaaggg agaggagccc cacatcaagg aagagatcca ggctgaggac
     4861 atcaacggag agatttacga ggagtacgat gaggaggagg aggacccgga tgtggattat
     4921 gggagtgaca gcgaaaacca cattgcggga caagccaact ctagagaggg caggggaagt
     4981 cttctaacat gcggggacgt ggaggaaaat cccggcccac tcgagatgac cgagtacaag
     5041 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5101 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5161 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5221 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5281 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5341 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5401 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5461 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     5521 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     5581 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgattaat taagctagct
     5641 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     5701 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     5761 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5821 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     5881 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     5941 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6001 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     6061 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6121 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6181 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6241 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     6301 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     6361 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     6421 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6481 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     6541 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     6601 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     6661 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     6721 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     6781 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6841 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6901 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     6961 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     7021 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7081 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     7141 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     7201 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     7261 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7321 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7381 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7441 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7501 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7561 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7621 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7681 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7741 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7801 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7861 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7921 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     7981 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     8041 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     8101 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     8161 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     8221 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     8281 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     8341 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     8401 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     8461 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     8521 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     8581 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     8641 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8701 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8761 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8821 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8881 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8941 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     9001 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9061 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9121 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9181 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9241 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9301 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9361 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9421 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9481 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9541 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9601 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9661 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9721 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9781 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9841 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9901 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9961 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    10021 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    10081 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    10141 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    10201 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    10261 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10321 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10381 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10441 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10501 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10561 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10621 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10681 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10741 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10801 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10861 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10921 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    10981 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
    11041 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    11101 aaaatttaag
//
