LOCUS       sequence-440258-       10798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses RUNX1T1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10798)
  AUTHORS   Bergmann T, Liu Y, Skov J, Mogus L, Lee J, Pfisterer U, Handfield 
            LF, Asenjo-Martinez A, Lisa-Vargas I, Seemann SE, Lee JTH, Patikas 
            N, Kornum BR, Denham M, Hyttel P, Witter MP, Gorodkin J, Pers TH, 
            Hemberg M, Khodosevich K, Hall VJ
  TITLE     Production of human entorhinal stellate cell-like cells by forward 
            programming shows an important role of Foxp1 in reprogramming.
  JOURNAL   Front Cell Dev Biol. 2022 Aug 15;10:976549. doi: 
            10.3389/fcell.2022.976549. eCollection 2022.
  PUBMED    36046338
REFERENCE   2  (bases 1 to 10798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Dev Biol."; date: "2022-08-15"; volume: "10:976549. doi"; pages: " 
            10.3389/fcell.2022.976549. eCollection 2022"
COMMENT     Sequence Label: p-Tet-O-RUNX1T1-Puro
FEATURES             Location/Qualifiers
     source          1..10798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        83..462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        464..662
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             680..860
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    907..1032
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1525..1758
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1943..1987
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2285..2402
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2459..2477,2478..2500,2501..2519,2520..2542,2543..2561,
                     2562..2584,2585..2603,2604..2626,2627..2645,2646..2668,
                     2669..2687,2688..2710,2711..2729)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2762..2800
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2797..2821
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4637..4690
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4697..5296
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4697..4716)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5193..5213
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5335..5923
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5388..5408)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5806..5817)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5926..5942)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5927..5943)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6448..6628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6654..6671)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6660..6884
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6930..7358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7017..7036)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7227..7248
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7367..7387)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7372..7701
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7552..7687
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7614..7633
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7749..7796
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7815..8189
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8319..8440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8356..8375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8410..8429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8489..8505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8489..8505)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8502..8524)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8513..8529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8537..8543,8544..8561,8562..8567))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8582..8603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8720..8737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8891..9479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8971..8990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9650..10441,10442..10510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10273..10292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10511..10615)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10690..10709)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
       61 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
      121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      661 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
      721 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
      781 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
      841 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
      901 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
      961 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     1021 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     1081 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     1141 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     1201 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     1261 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     1321 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     1381 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1441 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1501 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1561 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1621 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1681 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1741 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     1801 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     1861 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     1921 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     1981 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     2041 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     2101 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     2161 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     2221 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     2281 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2341 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2401 ttcgggttta ttacagggac agcagagatc cagtttggtt agatctcgag tttaccactc
     2461 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     2521 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     2581 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     2641 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2701 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagctcggt acccgggtcg
     2761 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     2821 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2881 cctccgcggc cccgaattcg ccaccatgcg tactgagaag cactccacaa tgccagactc
     2941 acctgtggat gtgaagacgc aatctaggct gactcctcca acaatgccac ctcccccaac
     3001 tactcaagga gctccaagaa ccagttcatt tacaccgaca acgttaacta atggcacgag
     3061 ccattctcct acagccttga atggcgcccc ctcaccaccc aatggcttca gcaatgggcc
     3121 ttcctcttct tcctcctcct ctctggctaa tcaacagctg cccccagcct gtggtgccag
     3181 gcaactcagc aagctgaaaa ggttccttac taccctgcag cagtttggca atgacatttc
     3241 acccgagata ggagaaagag ttcgcaccct cgttctggga ctagtgaact ccactttgac
     3301 aattgaagaa tttcattcca aactgcaaga agctactaac ttcccactga gaccttttgt
     3361 catcccattt ttgaaggcca acttgcccct gctgcagcgt gagctcctcc actgcgcaag
     3421 actggccaaa cagaaccctg cccagtacct cgcccagcat gaacagctgc ttctggatgc
     3481 cagcaccacc tcacctgttg actcctcaga gctgcttctc gatgtgaacg aaaacgggaa
     3541 gaggcgaact ccagacagaa ccaaagaaaa tggctttgac agagagcctt tgcactcaga
     3601 acatccaagc aagcgaccat gcactattag cccaggccag cggtacagtc caaataacgg
     3661 cttatcctac cagcccaatg gcctgcctca ccctacccca cctccacctc agcattaccg
     3721 tttggatgat atggccattg cccaccacta cagggactcc tatcgacacc ccagccacag
     3781 ggacctcagg gacagaaaca gacctatggg gttgcatggc acacgtcaag aagaaatgat
     3841 tgatcacaga ctaacagaca gagaatgggc agaagagtgg aaacatcttg accatctgtt
     3901 aaactgcata atggacatgg tagaaaaaac aaggcgatct ctcaccgtac taaggcggtg
     3961 tcaagaagca gaccgggaag aattgaatta ctggatccgg cggtacagtg acgccgagga
     4021 cttaaaaaaa ggtggcggca gtagcagcag ccactctagg cagcagagtc ccgtcaaccc
     4081 agacccagtt gcactagacg cgcatcggga attccttcac aggcctgcgt ctggatacgt
     4141 gccagaggag atctggaaga aagctgagga ggccgtcaat gaggtgaagc gccaggcgat
     4201 gacggagctg cagaaggccg tgtctgaggc ggagcggaaa gcccacgaca tgatcacaac
     4261 agagagggcc aagatggagc gcacggtcgc cgaggccaaa cggcaggcgg cggaggacgc
     4321 actggcagtt atcaatcagc aggaggattc aagcgagagt tgctggaatt gtggccgtaa
     4381 agcgagtgaa acctgcagtg gctgtaacac agcccgatac tgtggctcat tttgccagca
     4441 caaagactgg gagaagcacc atcacatctg tggacagacc ctgcaggccc agcagcaggg
     4501 agacacacct gcagtcagct cctctgtcac gcccaacagc ggggctggga gcccgatgga
     4561 cacaccacca gcagccactc cgaggtcaac caccccggga accccttcca ccatagagac
     4621 aacccctcgc tctagagagg gcaggggaag tcttctaaca tgcggggacg tggaggaaaa
     4681 tcccggccca ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     4741 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     4801 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     4861 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     4921 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     4981 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     5041 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     5101 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     5161 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     5221 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     5281 caagcccggt gcctgattaa ttaagctagc taattcgata tcaagcttat cgataatcaa
     5341 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5401 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5461 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5521 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5581 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5641 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     5701 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     5761 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     5821 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5881 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     5941 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     6001 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     6061 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6121 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     6181 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     6241 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     6301 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     6361 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     6421 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     6481 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6541 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6601 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     6661 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     6721 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     6781 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     6841 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     6901 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     6961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7021 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7081 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7141 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7201 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7261 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7321 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     7381 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7441 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7501 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7561 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7621 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     7681 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     7741 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     7801 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     7861 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     7921 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     7981 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     8041 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     8101 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     8161 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     8221 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     8281 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     8341 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8401 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     8461 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8521 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8581 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8641 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8701 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8761 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8821 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8881 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8941 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     9001 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9061 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9121 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9181 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9241 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9301 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9361 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9421 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9481 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9541 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9601 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9661 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9721 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9781 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9841 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9901 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9961 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10021 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10081 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10141 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10201 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10261 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10321 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10381 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10441 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10501 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10561 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10621 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
    10681 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
    10741 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggc
//
