LOCUS       sequence-440270-       11664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral 
            Replication: none.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11664)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 11664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_250d
FEATURES             Location/Qualifiers
     source          1..11664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1827..2852)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    4792..4813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4828..4833,4834..4851,4852..4858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4866..4882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4871..4893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4890..4906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4890..4906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4952..5076
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5101..5106,5107..5124,5125..5131)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5185..5865
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             6185..6490
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPASVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6369..6388
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6531..6655)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6675..6692)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6675..6691)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6684..6706)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6894..6918
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8219..8848
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9277..10350
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10416..10610
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10846..10868)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10954..11094
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11009..11028
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11109..11126)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(11280..11664,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11360..11379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt
     1861 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     1921 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     1981 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     2041 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc
     2101 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     2161 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     2221 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     2281 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     2341 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     2401 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     2461 ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga
     2521 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga acggcgggag
     2581 atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc
     2641 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     2701 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     2761 ccctccgaga gctgcatcag gtcggacacg ctgtcgaact tttcgatcag aaacttctcg
     2821 acagacgtcg cggtgagttc aggctttttc ataagcttct gcaaaagaga accagacaac
     2881 agggtaagtg cctagcagta aacaaacaga actcatcaca agcaaacagc aacatcatat
     2941 tcataccaac aggtcatgtg tgttcatcac atcattagta ctaagcatgc catcatccaa
     3001 gtatatcaaa gtaagggcaa agagcatgca tgatcatcag gtgcacaaaa gaatcatcaa
     3061 attgtagcag tacatatctt catctatcat gcatatctat ccataacagg acgatgcatg
     3121 ttgaccaggt aaaagctaca ggatcctata ggaacagcag cgtatatatc ttcaccaatc
     3181 ttagcatctt aatcatgtgg cacatgcagt ttcaatttaa gcacatgagc tagttgatta
     3241 tgaggtacca gagaatcatc aaactgatgt agcagcatat atcctcatct atcatgcaat
     3301 ctaatctaat ctaactaaac aggaaaggtg tgctattcag ttaaaagcta ccgcatcata
     3361 tacaaacggc agcataagaa aaagcataat catcttaatc atgcaacaaa cgcagattca
     3421 taataagccc aagagctagc ttgtgatgat cttattctac tctgatctac agcaatcaga
     3481 taacgaccta accttgcaca tggcaacaaa acaatcgatc ggacgaatca gttgtttgtt
     3541 cctagctagc accatcgaac cagataatag atgcacgtac agatcccgaa aacgaaccca
     3601 aaaacagggc agacctagct gaacctaggc agcgacccag cagatcgtga gaacgatctc
     3661 atctacgaac agcctagaag caaccccacg attcccggac aaacgaccta aaatccccac
     3721 aaatcacatg agcatgacag gataaacagc ggaaccgatc agatctacac gaaaacccca
     3781 cctcccagcc acccacgatc aggaaacacg cggatctagc atgatttcgt caacgcctca
     3841 gcctagttcc tagccacaga ccaagcagaa ccaccaaacc acgccgagcg aggagatggg
     3901 gcaagaggac gggggagacg atcgccgtac cttgaagcgg gggaaggatc gccgagggtc
     3961 gcgaggagag caatttggat ttggaacccg ggggttgtgc gctccgaacg atgagacgat
     4021 gtgagattgt gggaagaggc gcggagggcc ctgtatttat gggctgcgac ggggggagga
     4081 gaggtgggga gggttgggga aggaatcccc cacccgtgcc gtgacggttc cgggccgtgt
     4141 gagaggagcc cgctcgtctc cgccacgcaa tttccgcgat cggagcggag ctttcgagag
     4201 gcggctggat ggttggtggc cgttagattt gtagacgccg ttaacgcctc gcctccaccg
     4261 ggaagagttt tgagcagccg cttatgacaa tggcttaacg acgttagacg gagcgttagt
     4321 ggcaggccat gatagaatag acgtattgca atgggattat aattaaataa ataagaatat
     4381 aataagatat ggcaagtcgg cactcatgac atggtcttcg aaatgatagt gctcactttc
     4441 ttagccgaga aagttgacgc gactgattta gaagttaaga tattatttct ctcttctttt
     4501 ctttcctcgt catataagga tgaaataaac tttagagatt gccggtagtg attttggatt
     4561 tcggcgatca ggcttggttt gccggtttcg gacggtgtgc cttaggccac ccgcagtgta
     4621 tcttgtaatg ttcaaccgat aagcaagggt ggggctcaag caagtagtaa acaactatgt
     4681 caaatgtcac catggttatg gtcttgttta gttggcttct tggccgattc attaatgcag
     4741 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4801 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4861 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc
     4921 gagctcggta cccggggatc aaaagcaggt gacaagtttg tacaaaaaag ctgaacgaga
     4981 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     5041 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc
     5101 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
     5161 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     5221 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagtcgc tcaatgtacc
     5281 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     5341 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     5401 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     5461 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     5521 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     5581 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     5641 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     5701 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     5761 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     5821 gatgagtggc aggcggggcg taatctagag gatccggctt actaaaagcc agataacagt
     5881 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     5941 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     6001 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     6061 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     6121 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     6181 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     6241 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     6301 acgtctgctg tcagataaag tcccccgtga actttacccg gtggtgcata tcggggatga
     6361 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gcctccgtta tcggggaaga
     6421 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     6481 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     6541 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     6601 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtcacct
     6661 gcaaaaagtg tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6721 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6781 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc
     6841 ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg
     6901 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
     6961 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
     7021 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
     7081 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
     7141 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
     7201 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
     7261 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
     7321 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
     7381 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
     7441 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
     7501 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
     7561 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
     7621 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
     7681 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
     7741 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
     7801 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
     7861 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
     7921 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
     7981 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg
     8041 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
     8101 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
     8161 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
     8221 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
     8281 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
     8341 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
     8401 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
     8461 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
     8521 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
     8581 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
     8641 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
     8701 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
     8761 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
     8821 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
     8881 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
     8941 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
     9001 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
     9061 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
     9121 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
     9181 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg
     9241 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc
     9301 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
     9361 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
     9421 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
     9481 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
     9541 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
     9601 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
     9661 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
     9721 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
     9781 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
     9841 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
     9901 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
     9961 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
    10021 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
    10081 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
    10141 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
    10201 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
    10261 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
    10321 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
    10381 ctagcagggg aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac
    10441 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
    10501 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
    10561 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
    10621 cagcgcacag cccaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
    10681 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
    10741 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
    10801 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    10861 gctcccggaa acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    10921 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
    10981 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
    11041 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggccctct
    11101 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    11161 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    11221 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    11281 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    11341 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    11401 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    11461 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    11521 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    11581 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    11641 aacaggatta gcagagcgag gtat
//
