LOCUS       sequence-440535-       14705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expression of nptII and the N. nambi LUZ and CPH 
            genes flanked by insulators on the sides of the construction..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14705)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 14705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4665
FEATURES             Location/Qualifiers
     source          1..14705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(846..1098)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1138..1932)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1249..1268)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1859..1878
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1966..2145)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        3009..3353
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(3723..3734)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      4254..4506
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5350..5694
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     RBS             5822..5830
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      6712..6964
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8024..8048
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9349..9978
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10407..11480
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11546..11740
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11976..11998)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12084..12224
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12139..12158
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12239..12256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12410..12998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12490..12509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13086..13877)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    14663..14687
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagtggcta ttgttgttat catcaaaatc tatattgata ctattatact cgatatgtta
       61 tagactttat taaaaaaaac tttgtttgtt tgcagaaagt ggatttttaa tcaaatttaa
      121 ttgtgatgat tacaatgtca attggttatg caagatatta attaatatgt ttaagatttt
      181 tcacttttaa taagtaaaat aaaatacgtc aaaaaaattt cctaaagata atgccaaaaa
      241 ggttaaatta tagaaattaa actaaaatat attaaaagtt gacttaaaaa tgcaatgttg
      301 accaccgacc taggggaaaa caaatttacc gtatctggga tagaatctct tctgtatcat
      361 ctcagataaa gattactaca taaacacact ttacagtttt acgacgccca aaattcaatt
      421 cccggagatt aaaaacccta aaacaaacac tggcgattcg attttccctc ccaattcaaa
      481 tcatctctta atcttcttct tctcactatc ctctcaaatt cgccgccgat cttccatcaa
      541 ttttgatttc tcgcaccgaa atttcgactt ttcatctcct atcgacgaaa ccctaatttt
      601 tcacactcca gaaatcgaac tttgacgacg atgttaatgg ctaaaccccg ctgtcaggag
      661 tctcaatggt aactttactc tttatttaac catacatttt tttttatttt tttcactttg
      721 ttcttcatcc actattgttc tttgttcatc ttgaacaaaa gctccctcct tctttgttct
      781 tcatccacca ttgttcttca tcaatcattt cgctgtcagg agagcgatca gcttgcatgc
      841 cggtcgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
      901 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
      961 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
     1021 aaattatatg ataatcatgg caagaccggc aacaggattc aatcttaaga aactttattg
     1081 ccaaatgttt gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca
     1141 gaagaactcg tcaagaaggc gatagaaggc tatgcgctgc gaatcgggag cggcgatacc
     1201 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     1261 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     1321 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcgtcgccgt gggtcacgac
     1381 gagatcctcg ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     1441 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     1501 tgctcgctcg atgcggtgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     1561 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     1621 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     1681 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     1741 tgcctcgtct tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     1801 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     1861 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     1921 ttgttcaatc atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag
     1981 gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg
     2041 accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa
     2101 actccagaaa cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg
     2161 taaaacggct tgtcccgcgt catcggcggg ggtcataccg tgactccctt aattctcatg
     2221 tatctccgtc aggagtctca atggtaactt tactctttat ttaaccatac attttttttt
     2281 atttttttca ctttgttctt catccactat tgttctttgt tcatcttgaa caaaagctcc
     2341 ctccttcttt gttcttcatc caccattgtt cttcatcaat catttcgctg tcaggagact
     2401 agagccaagc tgatctcctt tgccccggag atcaccatgg acgactttct ctatctctac
     2461 gatctaggaa gaaagttcga cggagaaggt gacgatacca tgttcaccac cgataatgag
     2521 aagattagcc tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac
     2581 gcggcaggtc tgatcaagac gatctacccg agtaataatc tccaggagat caaatacctt
     2641 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
     2701 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     2761 cataaaccaa ggcaagtaat agagattgga gtctctaaga aagtagttcc tactgaatca
     2821 aaggccatgg agtcaaaaat tcagatcgag gatctaacag aactcgccgt gaagactggc
     2881 gaacagttca tacagagtct tttacgactc aatgacaaga agaaaatctt cgtcaacatg
     2941 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa
     3001 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc
     3061 ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc
     3121 catcattgcg ataaaggaaa ggctatcgtt caagatgccc ctgccgacag tggtcccaaa
     3181 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     3241 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     3301 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag gactccggta
     3361 tttttacaac aattaccaca acaaaacaaa caacaaacaa cattacaatt tactattcta
     3421 gtcgaaatga gaattaacat ctccctttca tcattatttg aacgtctgtc aaagttatcc
     3481 agcagatcca ttgcaatcac ctgtggtgtc gtcttggcct ccgcaatcgc ttttccgata
     3541 atccgtaggg actatcagac ttttttggaa gtcgggccga gttacgctcc gcagaatttc
     3601 agaggttaca tcatagtatg cgtgctttca ctctttaggc aggaacaaaa agggttggct
     3661 atctatgatc gactgcccga aaagcgaagg tggttggcag atctcccatt ccgagaaggt
     3721 acgcgtcctt ctatcacaag tcatatcatc caaagacaga ggactcagct ggtagaccaa
     3781 gaatttgcaa cccgagaact cattgataaa gttataccca gagttcaggc tcgacacacc
     3841 gataagacgt ttctgtcaac ctcaaagttc gaatttcatg ccaaagcaat ttttttgctg
     3901 cccagtattc ccatcaatga tcccttgaat ataccttcac atgatactgt cagacgaaca
     3961 aaaagggaga tcgctcacat gcacgattac cacgactgca ccctgcactt ggctctcgca
     4021 gcccaggacg gtaaagaagt acttaaaaaa gggtggggcc agagacatcc cctcgctggc
     4081 cccggcgtgc cgggacctcc aaccgaatgg acgtttttat atgcaccgcg taacgaggag
     4141 gaagccagag tggtcgagat gatagtggag gccagtattg gttacatgac aaatgacccc
     4201 gctggtaaaa ttgtagagaa cgctaagtga gcttggaatg gatcttcgat cccgatcgtt
     4261 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgacgatta
     4321 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     4381 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     4441 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
     4501 tagatcggga attgccaagc taattcttga agacgaaagg gcctcgtgat acgcctattt
     4561 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4621 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4681 atgagacaat aaccctgata aatgcttcaa taatgggacc gactcgcgct gtcaggagac
     4741 tagagccaag ctgatctcct ttgccccgga gatcaccatg gacgactttc tctatctcta
     4801 cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca ccgataatga
     4861 gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta gagaggccta
     4921 cgcggcaggt ctgatcaaga cgatctaccc gagtaataat ctccaggaga tcaaatacct
     4981 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     5041 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     5101 tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc ctactgaatc
     5161 aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg tgaagactgg
     5221 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
     5281 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
     5341 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
     5401 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
     5461 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc cctgccgaca gtggtcccaa
     5521 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     5581 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     5641 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggactccggt
     5701 atttttacaa caattaccac aacaaaacaa acaacaaaca acattacaat ttactattct
     5761 agtcgaaatg gccccaataa gttccacgtg gtctaggctc ataaggtttg tggctgtgga
     5821 aacctcctta gttcacatcg gcgagcctat tgatgcaaca atggacgtgg gcttagcacg
     5881 tagggaaggg aagacaatac aagcctatga gatcatcggc agtggctctg ctttagatct
     5941 gtctgcccaa gtgtcaaaga acgtacttac ggtccgtgag ctcttaatgc ccttgagccg
     6001 tgaggagatt aaaacggtaa gatgccttgg ccttaattat cccgtccacg ccacagaagc
     6061 caacgtggcc gtgccgaaat tcccgaacct tttttacaaa cccgtaacct ccctcatcgg
     6121 accaggtggg ttgataacca tcccttcagt tgtccaacca cctaaagagc accagagcga
     6181 ttacgaagcc gagttggtta tcgtcatcgg taaagccgcc aaaaatgtca gtgaagatga
     6241 agctctggac tacgtgttgg gctacaccgc agccaatgac atctcattta ggaaacatca
     6301 gttggccgtt tctcaatggt cattctccaa gggttttgat ggcacgaatc ccctgggacc
     6361 gtgcctggtt tctgcttcct ctattccaga tccacaggat attccgatcc aatgtaaact
     6421 caacggtggg gtggtgcaaa atggaaacac tcgtgatcag atttttaatg tcaagaaaac
     6481 aatctctttt ttaagccaag ggactacgtt ggagcctggt tccataatcc tgacggggac
     6541 gcccgacgga gtgggcttcg taagaaaccc accgctttat ttgaaggacg gtgatgaagt
     6601 aatgacttgg atcggatcag gtataggaac cttagccaac actgtccgag aggagcagac
     6661 ttgtttcgct tcaggagggc acgagtaagc ttggaatgga tcttcgatcc cgatcgttca
     6721 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gacgattatc
     6781 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     6841 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     6901 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     6961 gatcgggaat tgccaagcta attcttgaag acgaaagggc ctcgtgatac gcctattttt
     7021 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     7081 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7141 gagacaataa ccctgataaa tgcttcaata atgggaccga ctcgcgctgt caggagtggc
     7201 tattgttgtt atcatcaaaa tctatattga tactattata ctcgatatgt tatagacttt
     7261 attaaaaaaa actttgtttg tttgcagaaa gtggattttt aatcaaattt aattgtgatg
     7321 attacaatgt caattggtta tgcaagatat taattaatat gtttaagatt tttcactttt
     7381 aataagtaaa ataaaatacg tcaaaaaaat ttcctaaaga taatgccaaa aaggttaaat
     7441 tatagaaatt aaactaaaat atattaaaag ttgacttaaa aatgcaatgt tgaccaccga
     7501 cctaggggaa aacaaattta ccgtatctgg gatagaatct cttctgtatc atctcagata
     7561 aagattacta cataaacaca ctttacagtt ttacgacgcc caaaattcaa ttcccggaga
     7621 ttaaaaaccc taaaacaaac actggcgatt cgattttccc tcccaattca aatcatctct
     7681 taatcttctt cttctcacta tcctctcaaa ttcgccgccg atcttccatc aattttgatt
     7741 tctcgcaccg aaatttcgac ttttcatctc ctatcgacga aaccctaatt tttcacactc
     7801 cagaaatcga actttgacga cgatgttaat ggctaaaccc cgctgtcagg agtctcaatg
     7861 gtaactttac tctttattta accatacatt tttttttatt tttttcactt tgttcttcat
     7921 ccactattgt tctttgttca tcttgaacaa aagctccctc cttctttgtt cttcatccac
     7981 cattgttctt catcaatcat ttcgctgtca tgagaccgga tcctgacagg atatattggc
     8041 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
     8101 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     8161 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     8221 agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     8281 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     8341 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     8401 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     8461 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     8521 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     8581 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     8641 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     8701 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     8761 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     8821 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     8881 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     8941 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     9001 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     9061 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     9121 cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg cggctgcatg aaatcctggc
     9181 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     9241 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     9301 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     9361 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     9421 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     9481 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     9541 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     9601 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     9661 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     9721 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     9781 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     9841 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     9901 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     9961 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
    10021 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
    10081 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
    10141 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
    10201 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
    10261 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
    10321 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
    10381 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
    10441 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
    10501 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
    10561 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
    10621 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
    10681 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
    10741 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
    10801 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
    10861 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
    10921 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
    10981 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
    11041 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
    11101 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
    11161 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
    11221 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
    11281 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
    11341 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
    11401 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
    11461 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
    11521 aaaaaggtcg aaaaggactc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
    11581 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
    11641 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
    11701 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
    11761 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
    11821 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
    11881 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
    11941 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggtg
    12001 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    12061 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
    12121 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    12181 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
    12241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    12301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    12361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    12421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    12481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    12541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    12601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    12661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    12721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    12781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    12841 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    12901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    12961 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    13021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
    13081 ggtgattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
    13141 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
    13201 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
    13261 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
    13321 acatttcgct catcaccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
    13381 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
    13441 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
    13501 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
    13561 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
    13621 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
    13681 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
    13741 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
    13801 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
    13861 atcaccgctt ccctcataat gtttaacttt gttttagggc gactgccctg ctgcgtaaca
    13921 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
    13981 gcccgaggca tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact
    14041 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
    14101 tacttgcatt acagcttacg aaccgaacag gcttatgtcc actgggttcg tgccttcatc
    14161 cgtttccacg gtgtgcgtca cccggcaacc ttgggtagca gcgaagtcga ggcatttctg
    14221 tcctggctgg aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc
    14281 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
    14341 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
    14401 acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat
    14461 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
    14521 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
    14581 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
    14641 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacata acggatccgg
    14701 tctca
//
