LOCUS       sequence-440537-       17672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expression of nptII, the N. nambi CPH, LUZ and H3H 
            gene flankeds by insulators on both sides.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17672)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 17672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4405
FEATURES             Location/Qualifiers
     source          1..17672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1007..1259)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1299..2093)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1410..1429)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2020..2039
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2127..2306)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        3170..3514
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     RBS             3642..3650
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      4532..4784
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5628..5972
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(6342..6353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      6873..7125
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7969..8313
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      9679..9931
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10991..11015
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12316..12945
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13374..14447
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14513..14707
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14943..14965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    15051..15191
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     15106..15125
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15206..15223)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15377..15965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15457..15476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(16053..16844)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    17630..17654
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagctcaat ggtaacttta ctctttattt aaccatacat ttttttttat ttttttcact
       61 ttgttcttca tccactattg ttctttgttc atcttgaaca aaagctccct ccttctttgt
      121 tcttcatcca ccattgttct tcatcaatca tttcgctgtc aggagtggct attgttgtta
      181 tcatcaaaat ctatattgat actattatac tcgatatgtt atagacttta ttaaaaaaaa
      241 ctttgtttgt ttgcagaaag tggattttta atcaaattta attgtgatga ttacaatgtc
      301 aattggttat gcaagatatt aattaatatg tttaagattt ttcactttta ataagtaaaa
      361 taaaatacgt caaaaaaatt tcctaaagat aatgccaaaa aggttaaatt atagaaatta
      421 aactaaaata tattaaaagt tgacttaaaa atgcaatgtt gaccaccgac ctaggggaaa
      481 acaaatttac cgtatctggg atagaatctc ttctgtatca tctcagataa agattactac
      541 ataaacacac tttacagttt tacgacgccc aaaattcaat tcccggagat taaaaaccct
      601 aaaacaaaca ctggcgattc gattttccct cccaattcaa atcatctctt aatcttcttc
      661 ttctcactat cctctcaaat tcgccgccga tcttccatca attttgattt ctcgcaccga
      721 aatttcgact tttcatctcc tatcgacgaa accctaattt ttcacactcc agaaatcgaa
      781 ctttgacgac gatgttaatg gctaaacccc gctgtcagga gtctcaatgg taactttact
      841 ctttatttaa ccatacattt ttttttattt ttttcacttt gttcttcatc cactattgtt
      901 ctttgttcat cttgaacaaa agctccctcc ttctttgttc ttcatccacc attgttcttc
      961 atcaatcatt tcgctgtcag gagagcgatc agcttgcatg ccggtcgatc tagtaacata
     1021 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
     1081 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
     1141 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatg
     1201 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
     1261 gcttgactct agctagagtc cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     1321 cgatagaagg ctatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     1381 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     1441 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     1501 accatgatat tcggcaagca ggcgtcgccg tgggtcacga cgagatcctc gccgtcgggc
     1561 atccgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     1621 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcggtgt
     1681 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     1741 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     1801 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     1861 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttggagttca
     1921 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     1981 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     2041 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcctcga
     2101 tcgagttgag agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca
     2161 gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa
     2221 cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct
     2281 cagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg
     2341 tcatcggcgg gggtcatacc gtgactccct taattctcat gtatctccgt caggagtctc
     2401 aatggtaact ttactcttta tttaaccata catttttttt tatttttttc actttgttct
     2461 tcatccacta ttgttctttg ttcatcttga acaaaagctc cctccttctt tgttcttcat
     2521 ccaccattgt tcttcatcaa tcatttcgct gtcaggagac tagagccaag ctgatctcct
     2581 ttgccccgga gatcaccatg gacgactttc tctatctcta cgatctagga agaaagttcg
     2641 acggagaagg tgacgatacc atgttcacca ccgataatga gaagattagc ctcttcaatt
     2701 tcagaaagaa tgctgaccca cagatggtta gagaggccta cgcggcaggt ctgatcaaga
     2761 cgatctaccc gagtaataat ctccaggaga tcaaatacct tcccaagaag gttaaagatg
     2821 cagtcaaaag attcaggact aactgcatca agaacacaga gaaagatata tttctcaaga
     2881 tcagaagtac tattccagta tggacgattc aaggcttgct tcataaacca aggcaagtaa
     2941 tagagattgg agtctctaag aaagtagttc ctactgaatc aaaggccatg gagtcaaaaa
     3001 ttcagatcga ggatctaaca gaactcgccg tgaagactgg cgaacagttc atacagagtc
     3061 ttttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacactctcg
     3121 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
     3181 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
     3241 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
     3301 aggctatcgt tcaagatgcc cctgccgaca gtggtcccaa agatggaccc ccacccacga
     3361 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
     3421 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
     3481 ctatataagg aagttcattt catttggaga ggactccggt atttttacaa caattaccac
     3541 aacaaaacaa acaacaaaca acattacaat ttactattct agtcgaaatg gccccaataa
     3601 gttccacgtg gtctaggctc ataaggtttg tggctgtgga aacctcctta gttcacatcg
     3661 gcgagcctat tgatgcaaca atggacgtgg gcttagcacg tagggaaggg aagacaatac
     3721 aagcctatga gatcatcggc agtggctctg ctttagatct gtctgcccaa gtgtcaaaga
     3781 acgtacttac ggtccgtgag ctcttaatgc ccttgagccg tgaggagatt aaaacggtaa
     3841 gatgccttgg ccttaattat cccgtccacg ccacagaagc caacgtggcc gtgccgaaat
     3901 tcccgaacct tttttacaaa cccgtaacct ccctcatcgg accaggtggg ttgataacca
     3961 tcccttcagt tgtccaacca cctaaagagc accagagcga ttacgaagcc gagttggtta
     4021 tcgtcatcgg taaagccgcc aaaaatgtca gtgaagatga agctctggac tacgtgttgg
     4081 gctacaccgc agccaatgac atctcattta ggaaacatca gttggccgtt tctcaatggt
     4141 cattctccaa gggttttgat ggcacgaatc ccctgggacc gtgcctggtt tctgcttcct
     4201 ctattccaga tccacaggat attccgatcc aatgtaaact caacggtggg gtggtgcaaa
     4261 atggaaacac tcgtgatcag atttttaatg tcaagaaaac aatctctttt ttaagccaag
     4321 ggactacgtt ggagcctggt tccataatcc tgacggggac gcccgacgga gtgggcttcg
     4381 taagaaaccc accgctttat ttgaaggacg gtgatgaagt aatgacttgg atcggatcag
     4441 gtataggaac cttagccaac actgtccgag aggagcagac ttgtttcgct tcaggagggc
     4501 acgagtaagc ttggaatgga tcttcgatcc cgatcgttca aacatttggc aataaagttt
     4561 cttaagattg aatcctgttg ccggtcttgc gacgattatc atataatttc tgttgaatta
     4621 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     4681 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     4741 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tgccaagcta
     4801 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4861 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4921 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4981 tgcttcaata atgggaccga ctcgcgctgt caggagacta gagccaagct gatctccttt
     5041 gccccggaga tcaccatgga cgactttctc tatctctacg atctaggaag aaagttcgac
     5101 ggagaaggtg acgataccat gttcaccacc gataatgaga agattagcct cttcaatttc
     5161 agaaagaatg ctgacccaca gatggttaga gaggcctacg cggcaggtct gatcaagacg
     5221 atctacccga gtaataatct ccaggagatc aaataccttc ccaagaaggt taaagatgca
     5281 gtcaaaagat tcaggactaa ctgcatcaag aacacagaga aagatatatt tctcaagatc
     5341 agaagtacta ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata
     5401 gagattggag tctctaagaa agtagttcct actgaatcaa aggccatgga gtcaaaaatt
     5461 cagatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat acagagtctt
     5521 ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cactctcgtc
     5581 tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa
     5641 caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
     5701 aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag
     5761 gctatcgttc aagatgcccc tgccgacagt ggtcccaaag atggaccccc acccacgagg
     5821 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
     5881 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
     5941 atataaggaa gttcatttca tttggagagg actccggtat ttttacaaca attaccacaa
     6001 caaaacaaac aacaaacaac attacaattt actattctag tcgaaatgag aattaacatc
     6061 tccctttcat cattatttga acgtctgtca aagttatcca gcagatccat tgcaatcacc
     6121 tgtggtgtcg tcttggcctc cgcaatcgct tttccgataa tccgtaggga ctatcagact
     6181 tttttggaag tcgggccgag ttacgctccg cagaatttca gaggttacat catagtatgc
     6241 gtgctttcac tctttaggca ggaacaaaaa gggttggcta tctatgatcg actgcccgaa
     6301 aagcgaaggt ggttggcaga tctcccattc cgagaaggta cgcgtccttc tatcacaagt
     6361 catatcatcc aaagacagag gactcagctg gtagaccaag aatttgcaac ccgagaactc
     6421 attgataaag ttatacccag agttcaggct cgacacaccg ataagacgtt tctgtcaacc
     6481 tcaaagttcg aatttcatgc caaagcaatt tttttgctgc ccagtattcc catcaatgat
     6541 cccttgaata taccttcaca tgatactgtc agacgaacaa aaagggagat cgctcacatg
     6601 cacgattacc acgactgcac cctgcacttg gctctcgcag cccaggacgg taaagaagta
     6661 cttaaaaaag ggtggggcca gagacatccc ctcgctggcc ccggcgtgcc gggacctcca
     6721 accgaatgga cgtttttata tgcaccgcgt aacgaggagg aagccagagt ggtcgagatg
     6781 atagtggagg ccagtattgg ttacatgaca aatgaccccg ctggtaaaat tgtagagaac
     6841 gctaagtgag cttggaatgg atcttcgatc ccgatcgttc aaacatttgg caataaagtt
     6901 tcttaagatt gaatcctgtt gccggtcttg cgacgattat catataattt ctgttgaatt
     6961 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     7021 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     7081 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttgccaagct
     7141 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     7201 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7261 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7321 atgcttcaat aatgggaccg actcgcgctg tcaggagact agagccaagc tgatctcctt
     7381 tgccccggag atcaccatgg acgactttct ctatctctac gatctaggaa gaaagttcga
     7441 cggagaaggt gacgatacca tgttcaccac cgataatgag aagattagcc tcttcaattt
     7501 cagaaagaat gctgacccac agatggttag agaggcctac gcggcaggtc tgatcaagac
     7561 gatctacccg agtaataatc tccaggagat caaatacctt cccaagaagg ttaaagatgc
     7621 agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat
     7681 cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa ggcaagtaat
     7741 agagattgga gtctctaaga aagtagttcc tactgaatca aaggccatgg agtcaaaaat
     7801 tcagatcgag gatctaacag aactcgccgt gaagactggc gaacagttca tacagagtct
     7861 tttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acactctcgt
     7921 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
     7981 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     8041 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
     8101 ggctatcgtt caagatgccc ctgccgacag tggtcccaaa gatggacccc cacccacgag
     8161 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     8221 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
     8281 tatataagga agttcatttc atttggagag gactccggta tttttacaac aattaccaca
     8341 acaaaacaaa caacaaacaa cattacaatt tactattcta gtcgaaatgg catcctttga
     8401 aaatagtctg tccgttttga tcgtcggggc tggccttggg ggattagcag ccgcaatagc
     8461 tcttaggaga cagggccatg tagtaaagat ctatgatagt tcctcattca aagctgaact
     8521 gggtgctggc cttgctgtac ctccgaatac gcttagaagc cttcaacagt taggttgtaa
     8581 tacagagaat ttgaacggag tcgacaatct ctgcttcacg gccatgggtt acgacggctc
     8641 cgtaggtatg atgaataaca tgacagacta cagggaggct tatggcacat cctggattat
     8701 ggttcatcga gtggatttac ataatgaact tatgcgtgtc gcacttgatc cgggggggct
     8761 gggaccacca gcaacattac atttaaatca cagagtgacc ttctgcgacg ttgacgcatg
     8821 tacggtaact tttaccaacg ggacgacaca aagtgccgac ttgatcgtag gggctgacgg
     8881 catacgaagc accatacgta gattcgtctt ggaggaagat gtaactgtgc ctgccagcgg
     8941 gatcgtcggt ttcagatggc tggtgcaagc cgatgcctta gacccttacc ctgagttaga
     9001 ttggattgtt aagaagcctc cactcggtgc tcgtctgatc tctacgccac agaatccaca
     9061 gtccggcgtg gggttggctg accgtcgtac aattatcatc tacgcttgta ggggtggcac
     9121 gatggttaat gtgttggctg tccacgacga cgaaagagat cagaatacgg cagattggtc
     9181 agtgccggct tccaaagatg atctcttccg tgtattccat gactaccacc caagattcag
     9241 acgtttgctc gaacttgctc aagatatcaa tctctggcaa atgagggtag ttcccgtgct
     9301 taaaaaatgg gtgaacaagc gtgtctgttt attaggagat gccgcccatg cttctctccc
     9361 cacgctgggt caagggttcg gaatgggact ggaggacgcc gtggcactcg gcacattgtt
     9421 acccaaagga actacggctt cacagatcga aacgagactg gccgtttacg agcaattacg
     9481 aaaggatagg gctgagttcg tagcagcaga atcctacgag gaacagtatg tccctgagat
     9541 gcgtgggctc tatcttcgtt ccaaagaact ccgagatcga gtgatgggat atgatattaa
     9601 agtggagtca gaaaaggttt tggagactct gcttcgatcc tctaattctg catgagcttg
     9661 gaatggatct tcgatcccga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     9721 cctgttgccg gtcttgcgac gattatcata taatttctgt tgaattacgt taagcatgta
     9781 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     9841 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     9901 tcgcgcgcgg tgtcatctat gttactagat cgggaattgc caagctaatt cttgaagacg
     9961 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
    10021 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
    10081 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataatg
    10141 ggaccgactc gcgctgtcag gagtggctat tgttgttatc atcaaaatct atattgatac
    10201 tattatactc gatatgttat agactttatt aaaaaaaact ttgtttgttt gcagaaagtg
    10261 gatttttaat caaatttaat tgtgatgatt acaatgtcaa ttggttatgc aagatattaa
    10321 ttaatatgtt taagattttt cacttttaat aagtaaaata aaatacgtca aaaaaatttc
    10381 ctaaagataa tgccaaaaag gttaaattat agaaattaaa ctaaaatata ttaaaagttg
    10441 acttaaaaat gcaatgttga ccaccgacct aggggaaaac aaatttaccg tatctgggat
    10501 agaatctctt ctgtatcatc tcagataaag attactacat aaacacactt tacagtttta
    10561 cgacgcccaa aattcaattc ccggagatta aaaaccctaa aacaaacact ggcgattcga
    10621 ttttccctcc caattcaaat catctcttaa tcttcttctt ctcactatcc tctcaaattc
    10681 gccgccgatc ttccatcaat tttgatttct cgcaccgaaa tttcgacttt tcatctccta
    10741 tcgacgaaac cctaattttt cacactccag aaatcgaact ttgacgacga tgttaatggc
    10801 taaaccccgc tgtcaggagt ctcaatggta actttactct ttatttaacc atacattttt
    10861 ttttattttt ttcactttgt tcttcatcca ctattgttct ttgttcatct tgaacaaaag
    10921 ctccctcctt ctttgttctt catccaccat tgttcttcat caatcatttc gctgtcatga
    10981 gaccggatcc tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
    11041 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
    11101 gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata
    11161 gtgcagtcgg cttctgacgt tcagtgcagc cgtcatctga aaacgacatg tcgcacaagt
    11221 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt
    11281 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga
    11341 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca
    11401 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg
    11461 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg
    11521 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg
    11581 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca
    11641 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc
    11701 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga
    11761 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg
    11821 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
    11881 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
    11941 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
    12001 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
    12061 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacctctc
    12121 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
    12181 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
    12241 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
    12301 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
    12361 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
    12421 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
    12481 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
    12541 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
    12601 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
    12661 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
    12721 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
    12781 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
    12841 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
    12901 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
    12961 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
    13021 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
    13081 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
    13141 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
    13201 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
    13261 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
    13321 gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg
    13381 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
    13441 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
    13501 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
    13561 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
    13621 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
    13681 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
    13741 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
    13801 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
    13861 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
    13921 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
    13981 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
    14041 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
    14101 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
    14161 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
    14221 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
    14281 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
    14341 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
    14401 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
    14461 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggactcttt cctgtggata
    14521 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
    14581 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
    14641 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
    14701 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
    14761 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
    14821 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
    14881 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
    14941 aacctctgac acatgcagct cccggtgacg gtcacagctt gtctgtaagc ggatgccggg
    15001 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
    15061 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
    15121 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    15181 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    15241 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    15301 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    15361 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    15421 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    15481 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    15541 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    15601 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    15661 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    15721 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    15781 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    15841 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    15901 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    15961 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    16021 gttaagggat tttggtcatg cattctaggt gattatttgc cgactacctt ggtgatctcg
    16081 cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
    16141 tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg
    16201 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg
    16261 taccaaatgc gggacaacgt aagcactaca tttcgctcat caccagccca gtcgggcggc
    16321 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga
    16381 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
    16441 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca
    16501 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
    16561 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa
    16621 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
    16681 gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag
    16741 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc
    16801 tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcataatgtt taactttgtt
    16861 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
    16921 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaacag
    16981 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
    17041 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac cgaacaggct
    17101 tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg
    17161 ggtagcagcg aagtcgaggc atttctgtcc tggctggaac agaacttatt atttccttcc
    17221 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
    17281 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
    17341 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
    17401 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
    17461 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
    17521 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
    17581 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
    17641 attgtggtgt aaacataacg gatccggtct ca
//
