LOCUS       sequence-440538-       16326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the constitutive expression of the N. nambi LUZ, H3H 
            genes, EGFP and p19 fused to a SF in omega2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16326)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 16326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4468
FEATURES             Location/Qualifiers
     source          1..16326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        778..1122
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(1492..1503)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      2023..2275
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3119..3463
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      4829..5081
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5925..6269
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(6343..6345,6346..6348,6349..7059)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6343..6345,6346..6348,6349..7059)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6388..6409)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6649..6668)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6996..7017
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      7095..7347
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        8191..8535
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      9151..9403
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9641..9665
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10966..11595
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12024..13097
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13163..13357
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13593..13615)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13701..13841
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13756..13775
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13856..13873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14027..14615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14107..14126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14703..15494)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    16280..16304
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagtctcaa tggtaacttt actctttatt taaccataca ttttttttta tttttttcac
       61 tttgttcttc atccactatt gttctttgtt catcttgaac aaaagctccc tccttctttg
      121 ttcttcatcc accattgttc ttcatcaatc atttcgctgt caggagacta gagccaagct
      181 gatctccttt gccccggaga tcaccatgga cgactttctc tatctctacg atctaggaag
      241 aaagttcgac ggagaaggtg acgataccat gttcaccacc gataatgaga agattagcct
      301 cttcaatttc agaaagaatg ctgacccaca gatggttaga gaggcctacg cggcaggtct
      361 gatcaagacg atctacccga gtaataatct ccaggagatc aaataccttc ccaagaaggt
      421 taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga aagatatatt
      481 tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc ataaaccaag
      541 gcaagtaata gagattggag tctctaagaa agtagttcct actgaatcaa aggccatgga
      601 gtcaaaaatt cagatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat
      661 acagagtctt ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga
      721 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
      781 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
      841 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
      901 taaaggaaag gctatcgttc aagatgcccc tgccgacagt ggtcccaaag atggaccccc
      961 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     1021 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     1081 cccttcctct atataaggaa gttcatttca tttggagagg actccggtat ttttacaaca
     1141 attaccacaa caaaacaaac aacaaacaac attacaattt actattctag tcgaaatgag
     1201 aattaacatc tccctttcat cattatttga acgtctgtca aagttatcca gcagatccat
     1261 tgcaatcacc tgtggtgtcg tcttggcctc cgcaatcgct tttccgataa tccgtaggga
     1321 ctatcagact tttttggaag tcgggccgag ttacgctccg cagaatttca gaggttacat
     1381 catagtatgc gtgctttcac tctttaggca ggaacaaaaa gggttggcta tctatgatcg
     1441 actgcccgaa aagcgaaggt ggttggcaga tctcccattc cgagaaggta cgcgtccttc
     1501 tatcacaagt catatcatcc aaagacagag gactcagctg gtagaccaag aatttgcaac
     1561 ccgagaactc attgataaag ttatacccag agttcaggct cgacacaccg ataagacgtt
     1621 tctgtcaacc tcaaagttcg aatttcatgc caaagcaatt tttttgctgc ccagtattcc
     1681 catcaatgat cccttgaata taccttcaca tgatactgtc agacgaacaa aaagggagat
     1741 cgctcacatg cacgattacc acgactgcac cctgcacttg gctctcgcag cccaggacgg
     1801 taaagaagta cttaaaaaag ggtggggcca gagacatccc ctcgctggcc ccggcgtgcc
     1861 gggacctcca accgaatgga cgtttttata tgcaccgcgt aacgaggagg aagccagagt
     1921 ggtcgagatg atagtggagg ccagtattgg ttacatgaca aatgaccccg ctggtaaaat
     1981 tgtagagaac gctaagtgag cttggaatgg atcttcgatc ccgatcgttc aaacatttgg
     2041 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgacgattat catataattt
     2101 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     2161 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     2221 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     2281 ttgccaagct aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2341 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2401 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2461 accctgataa atgcttcaat aatgggaccg actcgcgctg tcaggagact agagccaagc
     2521 tgatctcctt tgccccggag atcaccatgg acgactttct ctatctctac gatctaggaa
     2581 gaaagttcga cggagaaggt gacgatacca tgttcaccac cgataatgag aagattagcc
     2641 tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac gcggcaggtc
     2701 tgatcaagac gatctacccg agtaataatc tccaggagat caaatacctt cccaagaagg
     2761 ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat
     2821 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa
     2881 ggcaagtaat agagattgga gtctctaaga aagtagttcc tactgaatca aaggccatgg
     2941 agtcaaaaat tcagatcgag gatctaacag aactcgccgt gaagactggc gaacagttca
     3001 tacagagtct tttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg
     3061 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg
     3121 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
     3181 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg
     3241 ataaaggaaa ggctatcgtt caagatgccc ctgccgacag tggtcccaaa gatggacccc
     3301 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     3361 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
     3421 acccttcctc tatataagga agttcatttc atttggagag gactccggta tttttacaac
     3481 aattaccaca acaaaacaaa caacaaacaa cattacaatt tactattcta gtcgaaatgg
     3541 catcctttga aaatagtctg tccgttttga tcgtcggggc tggccttggg ggattagcag
     3601 ccgcaatagc tcttaggaga cagggccatg tagtaaagat ctatgatagt tcctcattca
     3661 aagctgaact gggtgctggc cttgctgtac ctccgaatac gcttagaagc cttcaacagt
     3721 taggttgtaa tacagagaat ttgaacggag tcgacaatct ctgcttcacg gccatgggtt
     3781 acgacggctc cgtaggtatg atgaataaca tgacagacta cagggaggct tatggcacat
     3841 cctggattat ggttcatcga gtggatttac ataatgaact tatgcgtgtc gcacttgatc
     3901 cgggggggct gggaccacca gcaacattac atttaaatca cagagtgacc ttctgcgacg
     3961 ttgacgcatg tacggtaact tttaccaacg ggacgacaca aagtgccgac ttgatcgtag
     4021 gggctgacgg catacgaagc accatacgta gattcgtctt ggaggaagat gtaactgtgc
     4081 ctgccagcgg gatcgtcggt ttcagatggc tggtgcaagc cgatgcctta gacccttacc
     4141 ctgagttaga ttggattgtt aagaagcctc cactcggtgc tcgtctgatc tctacgccac
     4201 agaatccaca gtccggcgtg gggttggctg accgtcgtac aattatcatc tacgcttgta
     4261 ggggtggcac gatggttaat gtgttggctg tccacgacga cgaaagagat cagaatacgg
     4321 cagattggtc agtgccggct tccaaagatg atctcttccg tgtattccat gactaccacc
     4381 caagattcag acgtttgctc gaacttgctc aagatatcaa tctctggcaa atgagggtag
     4441 ttcccgtgct taaaaaatgg gtgaacaagc gtgtctgttt attaggagat gccgcccatg
     4501 cttctctccc cacgctgggt caagggttcg gaatgggact ggaggacgcc gtggcactcg
     4561 gcacattgtt acccaaagga actacggctt cacagatcga aacgagactg gccgtttacg
     4621 agcaattacg aaaggatagg gctgagttcg tagcagcaga atcctacgag gaacagtatg
     4681 tccctgagat gcgtgggctc tatcttcgtt ccaaagaact ccgagatcga gtgatgggat
     4741 atgatattaa agtggagtca gaaaaggttt tggagactct gcttcgatcc tctaattctg
     4801 catgagcttg gaatggatct tcgatcccga tcgttcaaac atttggcaat aaagtttctt
     4861 aagattgaat cctgttgccg gtcttgcgac gattatcata taatttctgt tgaattacgt
     4921 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     4981 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     5041 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattgc caagctaatt
     5101 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5161 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5281 ttcaataatg ggaccgactc gcgctgtcag gagactagag ccaagctgat ctcctttgcc
     5341 ccggagatca ccatggacga ctttctctat ctctacgatc taggaagaaa gttcgacgga
     5401 gaaggtgacg ataccatgtt caccaccgat aatgagaaga ttagcctctt caatttcaga
     5461 aagaatgctg acccacagat ggttagagag gcctacgcgg caggtctgat caagacgatc
     5521 tacccgagta ataatctcca ggagatcaaa taccttccca agaaggttaa agatgcagtc
     5581 aaaagattca ggactaactg catcaagaac acagagaaag atatatttct caagatcaga
     5641 agtactattc cagtatggac gattcaaggc ttgcttcata aaccaaggca agtaatagag
     5701 attggagtct ctaagaaagt agttcctact gaatcaaagg ccatggagtc aaaaattcag
     5761 atcgaggatc taacagaact cgccgtgaag actggcgaac agttcataca gagtctttta
     5821 cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac tctcgtctac
     5881 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
     5941 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
     6001 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
     6061 atcgttcaag atgcccctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     6121 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     6181 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     6241 taaggaagtt catttcattt ggagaggact ccggtatttt tacaacaatt accacaacaa
     6301 aacaaacaac aaacaacatt acaatttact attctagtcg aaatggtgag caagggcgag
     6361 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     6421 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     6481 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     6541 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     6601 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     6661 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     6721 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     6781 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     6841 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     6901 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     6961 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     7021 gccgccggga tcactctcgg catggacgag ctgtacaagg acgagctgta agcttggaat
     7081 ggatcttcga tcccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     7141 ttgccggtct tgcgacgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     7201 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     7261 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     7321 gcgcggtgtc atctatgtta ctagatcggg aattgccaag ctaattcttg aagacgaaag
     7381 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     7441 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7501 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatgggac
     7561 cgactcgcgc tgtcaggaga ctagagccaa gctgatctcc tttgccccgg agatcaccat
     7621 ggacgacttt ctctatctct acgatctagg aagaaagttc gacggagaag gtgacgatac
     7681 catgttcacc accgataatg agaagattag cctcttcaat ttcagaaaga atgctgaccc
     7741 acagatggtt agagaggcct acgcggcagg tctgatcaag acgatctacc cgagtaataa
     7801 tctccaggag atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac
     7861 taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt
     7921 atggacgatt caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa
     7981 gaaagtagtt cctactgaat caaaggccat ggagtcaaaa attcagatcg aggatctaac
     8041 agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa
     8101 gaagaaaatc ttcgtcaaca tggtggagca cgacactctc gtctactcca agaatatcaa
     8161 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg
     8221 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
     8281 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
     8341 ccctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     8401 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     8461 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     8521 tcatttggag aggactccgg tatttttaca acaattacca caacaaaaca aacaacaaac
     8581 aacattacaa tttactattc tagtcgaaat ggaacgagct atacaaggaa acgacgctag
     8641 ggaacaagct aacagtgaac gttgggatgg aggatcagga ggtaccactt ctcccttcaa
     8701 acttcctgac gaaagtccga gttggactga gtggcggcta cataacgatg agaccaattc
     8761 gaatcaagat aatccccttg gtttcaagga aagctggggt ttcgggaaag ttgtatttaa
     8821 gagatatctc agatacgaca ggacggaagc ttcactgcac agagtccttg gatcttggac
     8881 gggagattcg gttaactatg cagcatctcg atttttcggt ttcgaccaga tcggatgtac
     8941 ctatagtatt cggtttcgag gagttagtat caccgtttct ggaggctctc gaactcttca
     9001 gcatctctgt gagatggcaa ttcggtctaa gcaagaactg ctacagcttg ccccaatcga
     9061 agtggaaagt aatgtatcaa gaggatgccc tgaaggtact gaaaccttcg aaaaagaaag
     9121 cgagtgagct tggaatggat cttcgatccc gatcgttcaa acatttggca ataaagtttc
     9181 ttaagattga atcctgttgc cggtcttgcg acgattatca tataatttct gttgaattac
     9241 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     9301 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     9361 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt gccaagctaa
     9421 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     9481 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9541 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9601 gcttcaataa tgggaccgac tcgcgcttga gaccgatatc tgacaggata tattggcggg
     9661 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     9721 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa
     9781 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
     9841 cgtcatctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
     9901 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
     9961 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
    10021 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
    10081 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
    10141 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
    10201 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
    10261 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
    10321 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
    10381 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
    10441 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
    10501 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
    10561 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
    10621 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
    10681 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
    10741 gtacgtgttc gagccgcccg cgcacctctc aaccgtgcgg ctgcatgaaa tcctggccgg
    10801 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
    10861 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
    10921 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
    10981 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
    11041 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
    11101 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
    11161 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
    11221 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
    11281 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
    11341 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
    11401 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
    11461 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
    11521 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
    11581 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
    11641 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
    11701 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
    11761 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
    11821 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
    11881 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
    11941 acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa tggcactgga
    12001 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
    12061 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
    12121 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
    12181 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
    12241 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
    12301 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
    12361 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
    12421 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
    12481 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
    12541 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
    12601 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
    12661 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
    12721 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
    12781 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
    12841 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
    12901 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
    12961 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
    13021 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
    13081 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
    13141 aaggtcgaaa aggactcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
    13201 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
    13261 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
    13321 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
    13381 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
    13441 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
    13501 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
    13561 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggtgacg
    13621 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
    13681 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
    13741 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
    13801 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
    13861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    13921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    13981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    14041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    14101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    14161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    14221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    14281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    14341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    14401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    14461 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    14521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    14581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    14641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
    14701 gattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac
    14761 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
    14821 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc
    14881 ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca
    14941 tttcgctcat caccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc
    15001 gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc
    15061 aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg
    15121 gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg
    15181 cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca
    15241 gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa
    15301 gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg
    15361 tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt
    15421 tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc
    15481 accgcttccc tcataatgtt taactttgtt ttagggcgac tgccctgctg cgtaacatcg
    15541 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
    15601 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
    15661 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
    15721 ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt
    15781 ttccacggtg tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc atttctgtcc
    15841 tggctggaac agaacttatt atttccttcc tcttttctac agtatttaaa gataccccaa
    15901 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
    15961 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
    16021 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
    16081 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
    16141 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
    16201 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
    16261 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacataacg atatcggtct
    16321 cagtca
//
