LOCUS       sequence-440539-       18616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the constitutive expression of the N. nambi CPH, LUZ, H3H
            genes, EGFP and p19 fused to a SF in alpha2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18616)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 18616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4485
FEATURES             Location/Qualifiers
     source          1..18616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        778..1122
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     RBS             1250..1258
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      2140..2392
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3398..3742
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(4112..4123)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      4643..4895
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        5739..6083
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      7449..7701
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        8545..8889
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(8963..8965,8966..8968,8969..9679)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8963..8965,8966..8968,8969..9679)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9008..9029)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9269..9288)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9616..9637
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      9715..9967
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        10811..11155
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      11771..12023
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    12260..12284
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             13585..14214
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             14643..15716
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      15782..15976
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(16212..16234)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    16320..16460
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     16375..16394
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(16475..16492)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16646..17234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16726..16745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(17322..18137)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(17322..18137)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     18045..18064
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    18570..18594
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagtctcaa tggtaacttt actctttatt taaccataca ttttttttta tttttttcac
       61 tttgttcttc atccactatt gttctttgtt catcttgaac aaaagctccc tccttctttg
      121 ttcttcatcc accattgttc ttcatcaatc atttcgctgt caggagacta gagccaagct
      181 gatctccttt gccccggaga tcaccatgga cgactttctc tatctctacg atctaggaag
      241 aaagttcgac ggagaaggtg acgataccat gttcaccacc gataatgaga agattagcct
      301 cttcaatttc agaaagaatg ctgacccaca gatggttaga gaggcctacg cggcaggtct
      361 gatcaagacg atctacccga gtaataatct ccaggagatc aaataccttc ccaagaaggt
      421 taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga aagatatatt
      481 tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc ataaaccaag
      541 gcaagtaata gagattggag tctctaagaa agtagttcct actgaatcaa aggccatgga
      601 gtcaaaaatt cagatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat
      661 acagagtctt ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga
      721 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
      781 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
      841 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
      901 taaaggaaag gctatcgttc aagatgcccc tgccgacagt ggtcccaaag atggaccccc
      961 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     1021 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     1081 cccttcctct atataaggaa gttcatttca tttggagagg actccggtat ttttacaaca
     1141 attaccacaa caaaacaaac aacaaacaac attacaattt actattctag tcgaaatggc
     1201 cccaataagt tccacgtggt ctaggctcat aaggtttgtg gctgtggaaa cctccttagt
     1261 tcacatcggc gagcctattg atgcaacaat ggacgtgggc ttagcacgta gggaagggaa
     1321 gacaatacaa gcctatgaga tcatcggcag tggctctgct ttagatctgt ctgcccaagt
     1381 gtcaaagaac gtacttacgg tccgtgagct cttaatgccc ttgagccgtg aggagattaa
     1441 aacggtaaga tgccttggcc ttaattatcc cgtccacgcc acagaagcca acgtggccgt
     1501 gccgaaattc ccgaaccttt tttacaaacc cgtaacctcc ctcatcggac caggtgggtt
     1561 gataaccatc ccttcagttg tccaaccacc taaagagcac cagagcgatt acgaagccga
     1621 gttggttatc gtcatcggta aagccgccaa aaatgtcagt gaagatgaag ctctggacta
     1681 cgtgttgggc tacaccgcag ccaatgacat ctcatttagg aaacatcagt tggccgtttc
     1741 tcaatggtca ttctccaagg gttttgatgg cacgaatccc ctgggaccgt gcctggtttc
     1801 tgcttcctct attccagatc cacaggatat tccgatccaa tgtaaactca acggtggggt
     1861 ggtgcaaaat ggaaacactc gtgatcagat ttttaatgtc aagaaaacaa tctctttttt
     1921 aagccaaggg actacgttgg agcctggttc cataatcctg acggggacgc ccgacggagt
     1981 gggcttcgta agaaacccac cgctttattt gaaggacggt gatgaagtaa tgacttggat
     2041 cggatcaggt ataggaacct tagccaacac tgtccgagag gagcagactt gtttcgcttc
     2101 aggagggcac gagtaagctt ggaatggatc ttcgatcccg atcgttcaaa catttggcaa
     2161 taaagtttct taagattgaa tcctgttgcc ggtcttgcga cgattatcat ataatttctg
     2221 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     2281 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     2341 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     2401 ccaagctaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     2461 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     2521 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2581 ctgataaatg cttcaataat gggaccgact cgcgctgtca ggagtctcaa tggtaacttt
     2641 actctttatt taaccataca ttttttttta tttttttcac tttgttcttc atccactatt
     2701 gttctttgtt catcttgaac aaaagctccc tccttctttg ttcttcatcc accattgttc
     2761 ttcatcaatc atttcgctgt caggagacta gagccaagct gatctccttt gccccggaga
     2821 tcaccatgga cgactttctc tatctctacg atctaggaag aaagttcgac ggagaaggtg
     2881 acgataccat gttcaccacc gataatgaga agattagcct cttcaatttc agaaagaatg
     2941 ctgacccaca gatggttaga gaggcctacg cggcaggtct gatcaagacg atctacccga
     3001 gtaataatct ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     3061 tcaggactaa ctgcatcaag aacacagaga aagatatatt tctcaagatc agaagtacta
     3121 ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag
     3181 tctctaagaa agtagttcct actgaatcaa aggccatgga gtcaaaaatt cagatcgagg
     3241 atctaacaga actcgccgtg aagactggcg aacagttcat acagagtctt ttacgactca
     3301 atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cactctcgtc tactccaaga
     3361 atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
     3421 tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag
     3481 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc
     3541 aagatgcccc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     3601 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
     3661 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     3721 gttcatttca tttggagagg actccggtat ttttacaaca attaccacaa caaaacaaac
     3781 aacaaacaac attacaattt actattctag tcgaaatgag aattaacatc tccctttcat
     3841 cattatttga acgtctgtca aagttatcca gcagatccat tgcaatcacc tgtggtgtcg
     3901 tcttggcctc cgcaatcgct tttccgataa tccgtaggga ctatcagact tttttggaag
     3961 tcgggccgag ttacgctccg cagaatttca gaggttacat catagtatgc gtgctttcac
     4021 tctttaggca ggaacaaaaa gggttggcta tctatgatcg actgcccgaa aagcgaaggt
     4081 ggttggcaga tctcccattc cgagaaggta cgcgtccttc tatcacaagt catatcatcc
     4141 aaagacagag gactcagctg gtagaccaag aatttgcaac ccgagaactc attgataaag
     4201 ttatacccag agttcaggct cgacacaccg ataagacgtt tctgtcaacc tcaaagttcg
     4261 aatttcatgc caaagcaatt tttttgctgc ccagtattcc catcaatgat cccttgaata
     4321 taccttcaca tgatactgtc agacgaacaa aaagggagat cgctcacatg cacgattacc
     4381 acgactgcac cctgcacttg gctctcgcag cccaggacgg taaagaagta cttaaaaaag
     4441 ggtggggcca gagacatccc ctcgctggcc ccggcgtgcc gggacctcca accgaatgga
     4501 cgtttttata tgcaccgcgt aacgaggagg aagccagagt ggtcgagatg atagtggagg
     4561 ccagtattgg ttacatgaca aatgaccccg ctggtaaaat tgtagagaac gctaagtgag
     4621 cttggaatgg atcttcgatc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     4681 gaatcctgtt gccggtcttg cgacgattat catataattt ctgttgaatt acgttaagca
     4741 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     4801 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     4861 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttgccaagct aattcttgaa
     4921 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4981 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5041 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5101 aatgggaccg actcgcgctg tcaggagact agagccaagc tgatctcctt tgccccggag
     5161 atcaccatgg acgactttct ctatctctac gatctaggaa gaaagttcga cggagaaggt
     5221 gacgatacca tgttcaccac cgataatgag aagattagcc tcttcaattt cagaaagaat
     5281 gctgacccac agatggttag agaggcctac gcggcaggtc tgatcaagac gatctacccg
     5341 agtaataatc tccaggagat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga
     5401 ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat cagaagtact
     5461 attccagtat ggacgattca aggcttgctt cataaaccaa ggcaagtaat agagattgga
     5521 gtctctaaga aagtagttcc tactgaatca aaggccatgg agtcaaaaat tcagatcgag
     5581 gatctaacag aactcgccgt gaagactggc gaacagttca tacagagtct tttacgactc
     5641 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acactctcgt ctactccaag
     5701 aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     5761 atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca
     5821 gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt
     5881 caagatgccc ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     5941 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact
     6001 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     6061 agttcatttc atttggagag gactccggta tttttacaac aattaccaca acaaaacaaa
     6121 caacaaacaa cattacaatt tactattcta gtcgaaatgg catcctttga aaatagtctg
     6181 tccgttttga tcgtcggggc tggccttggg ggattagcag ccgcaatagc tcttaggaga
     6241 cagggccatg tagtaaagat ctatgatagt tcctcattca aagctgaact gggtgctggc
     6301 cttgctgtac ctccgaatac gcttagaagc cttcaacagt taggttgtaa tacagagaat
     6361 ttgaacggag tcgacaatct ctgcttcacg gccatgggtt acgacggctc cgtaggtatg
     6421 atgaataaca tgacagacta cagggaggct tatggcacat cctggattat ggttcatcga
     6481 gtggatttac ataatgaact tatgcgtgtc gcacttgatc cgggggggct gggaccacca
     6541 gcaacattac atttaaatca cagagtgacc ttctgcgacg ttgacgcatg tacggtaact
     6601 tttaccaacg ggacgacaca aagtgccgac ttgatcgtag gggctgacgg catacgaagc
     6661 accatacgta gattcgtctt ggaggaagat gtaactgtgc ctgccagcgg gatcgtcggt
     6721 ttcagatggc tggtgcaagc cgatgcctta gacccttacc ctgagttaga ttggattgtt
     6781 aagaagcctc cactcggtgc tcgtctgatc tctacgccac agaatccaca gtccggcgtg
     6841 gggttggctg accgtcgtac aattatcatc tacgcttgta ggggtggcac gatggttaat
     6901 gtgttggctg tccacgacga cgaaagagat cagaatacgg cagattggtc agtgccggct
     6961 tccaaagatg atctcttccg tgtattccat gactaccacc caagattcag acgtttgctc
     7021 gaacttgctc aagatatcaa tctctggcaa atgagggtag ttcccgtgct taaaaaatgg
     7081 gtgaacaagc gtgtctgttt attaggagat gccgcccatg cttctctccc cacgctgggt
     7141 caagggttcg gaatgggact ggaggacgcc gtggcactcg gcacattgtt acccaaagga
     7201 actacggctt cacagatcga aacgagactg gccgtttacg agcaattacg aaaggatagg
     7261 gctgagttcg tagcagcaga atcctacgag gaacagtatg tccctgagat gcgtgggctc
     7321 tatcttcgtt ccaaagaact ccgagatcga gtgatgggat atgatattaa agtggagtca
     7381 gaaaaggttt tggagactct gcttcgatcc tctaattctg catgagcttg gaatggatct
     7441 tcgatcccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     7501 gtcttgcgac gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     7561 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     7621 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     7681 tgtcatctat gttactagat cgggaattgc caagctaatt cttgaagacg aaagggcctc
     7741 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     7801 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7861 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataatg ggaccgactc
     7921 gcgctgtcag gagactagag ccaagctgat ctcctttgcc ccggagatca ccatggacga
     7981 ctttctctat ctctacgatc taggaagaaa gttcgacgga gaaggtgacg ataccatgtt
     8041 caccaccgat aatgagaaga ttagcctctt caatttcaga aagaatgctg acccacagat
     8101 ggttagagag gcctacgcgg caggtctgat caagacgatc tacccgagta ataatctcca
     8161 ggagatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg
     8221 catcaagaac acagagaaag atatatttct caagatcaga agtactattc cagtatggac
     8281 gattcaaggc ttgcttcata aaccaaggca agtaatagag attggagtct ctaagaaagt
     8341 agttcctact gaatcaaagg ccatggagtc aaaaattcag atcgaggatc taacagaact
     8401 cgccgtgaag actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa
     8461 aatcttcgtc aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac
     8521 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct
     8581 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
     8641 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcccctgc
     8701 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     8761 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
     8821 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     8881 ggagaggact ccggtatttt tacaacaatt accacaacaa aacaaacaac aaacaacatt
     8941 acaatttact attctagtcg aaatggtgag caagggcgag gagctgttca ccggggtggt
     9001 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     9061 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     9121 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     9181 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     9241 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     9301 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     9361 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     9421 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     9481 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     9541 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     9601 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     9661 catggacgag ctgtacaagg acgagctgta agcttggaat ggatcttcga tcccgatcgt
     9721 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgacgatt
     9781 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     9841 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     9901 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     9961 ctagatcggg aattgccaag ctaattcttg aagacgaaag ggcctcgtga tacgcctatt
    10021 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
    10081 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    10141 catgagacaa taaccctgat aaatgcttca ataatgggac cgactcgcgc tgtcaggaga
    10201 ctagagccaa gctgatctcc tttgccccgg agatcaccat ggacgacttt ctctatctct
    10261 acgatctagg aagaaagttc gacggagaag gtgacgatac catgttcacc accgataatg
    10321 agaagattag cctcttcaat ttcagaaaga atgctgaccc acagatggtt agagaggcct
    10381 acgcggcagg tctgatcaag acgatctacc cgagtaataa tctccaggag atcaaatacc
    10441 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag
    10501 agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
    10561 ttcataaacc aaggcaagta atagagattg gagtctctaa gaaagtagtt cctactgaat
    10621 caaaggccat ggagtcaaaa attcagatcg aggatctaac agaactcgcc gtgaagactg
    10681 gcgaacagtt catacagagt cttttacgac tcaatgacaa gaagaaaatc ttcgtcaaca
    10741 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
    10801 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
    10861 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
    10921 gccatcattg cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca
    10981 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
    11041 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
    11101 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg
    11161 tatttttaca acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc
    11221 tagtcgaaat ggaacgagct atacaaggaa acgacgctag ggaacaagct aacagtgaac
    11281 gttgggatgg aggatcagga ggtaccactt ctcccttcaa acttcctgac gaaagtccga
    11341 gttggactga gtggcggcta cataacgatg agaccaattc gaatcaagat aatccccttg
    11401 gtttcaagga aagctggggt ttcgggaaag ttgtatttaa gagatatctc agatacgaca
    11461 ggacggaagc ttcactgcac agagtccttg gatcttggac gggagattcg gttaactatg
    11521 cagcatctcg atttttcggt ttcgaccaga tcggatgtac ctatagtatt cggtttcgag
    11581 gagttagtat caccgtttct ggaggctctc gaactcttca gcatctctgt gagatggcaa
    11641 ttcggtctaa gcaagaactg ctacagcttg ccccaatcga agtggaaagt aatgtatcaa
    11701 gaggatgccc tgaaggtact gaaaccttcg aaaaagaaag cgagtgagct tggaatggat
    11761 cttcgatccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
    11821 cggtcttgcg acgattatca tataatttct gttgaattac gttaagcatg taataattaa
    11881 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
    11941 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
    12001 ggtgtcatct atgttactag atcgggaatt gccaagctaa ttcttgaaga cgaaagggcc
    12061 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    12121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    12181 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tgggaccgac
    12241 tcgcgcttga gacgaagctt gacaggatat attggcgggt aaacctaaga gaaaagagcg
    12301 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
    12361 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
    12421 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt
    12481 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
    12541 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
    12601 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
    12661 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
    12721 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
    12781 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
    12841 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
    12901 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
    12961 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
    13021 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
    13081 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
    13141 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
    13201 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
    13261 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
    13321 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
    13381 gcacctctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
    13441 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
    13501 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
    13561 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
    13621 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
    13681 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
    13741 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
    13801 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
    13861 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
    13921 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
    13981 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
    14041 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
    14101 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
    14161 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
    14221 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
    14281 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
    14341 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
    14401 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
    14461 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
    14521 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
    14581 agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
    14641 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
    14701 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
    14761 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
    14821 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
    14881 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
    14941 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
    15001 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
    15061 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
    15121 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
    15181 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
    15241 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
    15301 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
    15361 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
    15421 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
    15481 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
    15541 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
    15601 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
    15661 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
    15721 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggactctttc
    15781 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
    15841 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
    15901 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
    15961 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
    16021 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
    16081 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
    16141 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
    16201 gacggtgaaa acctctgaca catgcagctc ccggtgacgg tcacagcttg tctgtaagcg
    16261 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    16321 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
    16381 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
    16441 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    16501 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    16561 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    16621 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    16681 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    16741 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    16801 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    16861 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    16921 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    16981 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    17041 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    17101 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    17161 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    17221 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    17281 aaaactcacg ttaagggatt ttggtcatgc attctaggtg attagaaaaa ctcatcgagc
    17341 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
    17401 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
    17461 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
    17521 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
    17581 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
    17641 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagtcgaaat
    17701 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
    17761 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
    17821 gctgttttcc ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
    17881 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
    17941 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
    18001 ttcccataca atcggtagat tgtcgcacct gattgcccga cattatcgcg agcccattta
    18061 tacccatata aatcagcatc catgttggaa tttaatcgcg gccttgagca agacgtttcc
    18121 cgttgaatat ggctcataac agaacttatt atttccttcc tcttttctac agtatttaaa
    18181 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
    18241 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
    18301 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
    18361 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
    18421 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
    18481 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
    18541 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacataaca
    18601 agcttcgtct cagtca
//
