LOCUS       sequence-440573-       20237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the constitutive expression of HISPS, H3H, EGFP and p23..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20237)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 20237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4832
FEATURES             Location/Qualifiers
     source          1..20237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        616..960
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      6154..6406
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7250..7594
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      8960..9212
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        10056..10400
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(10474..10476,10477..10479,10480..11190)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(10474..10476,10477..10479,10480..11190)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10519..10540)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10780..10799)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11127..11148
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      11226..11478
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        12322..12666
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      13393..13645
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    13886..13910
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             15211..15840
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             16269..17342
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      17408..17602
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(17838..17860)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    17946..18086
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     18001..18020
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(18101..18118)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(18272..18860)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18352..18371)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(18948..19763)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(18948..19763)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     19671..19690
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    20196..20220
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcccctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat tacaatttac
     1021 tattctagtc gaaatgaact caagtaaaaa tcctccttct actctccttg atgttttctt
     1081 ggataccgca agaaacttgg atacagcaag cagaaatgtg ctcgagtgtg gtgaacatcg
     1141 atggagttac agagaactgg atacagtgtc ctcagctctc gcccagcatc ttcgatacac
     1201 ggtggggctg tcccctactg tcgcagtgat cagcgagaat cacccgtaca tattggccct
     1261 catgctcgct gtatggaaat tgggcggtac cttcgctccg atagatgtcc acagccctgc
     1321 agaactggtt gccgggatgc ttaacatagt ctcaccgtct tgcctggtca tcccgtcatc
     1381 agacgtaaca aaccagaccc ttgcatgtga tcttaacatt ccagtggttg catttcatcc
     1441 tcaccaaagc acaatcccgg aactgaacaa aaagtacctg acggacagtc aaatcagccc
     1501 tgatctccca ttccccgatc ccaaccgtcc ggctctttac ctgttcactt cttctgctac
     1561 gagtagatcc aacttgaagt gcgtgcccct gacccacacc ttcatactga gaaatagcct
     1621 gtcaaagagg gcctggtgca aaagaatgag gccagaaaca gatttcgatg gtataagggt
     1681 tttaggatgg gcaccatgga gtcatgttct ggctcacatg caagatatag ggccccttac
     1741 actcctgaat gctggatgtt acgtctttgc aacgacaccg agcacatatc caaccgagtt
     1801 gaaggacgat agggatgtca tttcttgcgc cgcaaatgca gttatgtaca agggggttaa
     1861 gtccttcgcc tgtctcccgt ttgtgctggg aggccttaag gccctgtgtg agtccgagcc
     1921 gtccgtcaaa gcacaattac aggtcgagga gcgagcacag ttgttaaaga gcttacaaca
     1981 catgaagatt cttgaatgtg gtggcgctat gctcgaagtt tcagtggctt cttgggctat
     2041 cgagaatcgt attcccatta gtataggaat cggtatgacc gaaactggcg gagccttgtt
     2101 cgctggaccc gttcaagcaa tccaaacggg atttagttca gaggacaagt ttattgagga
     2161 tgccacatat ctccttgtca aagacgatta cgagtcacac gcagaagagg acatcaatga
     2221 aggcgaactg gttgttaaaa gccgaatgct gccaaggggt tatctggggt ataacgatcc
     2281 atccttctca gtggacgatg cagggtgggt taccttcaaa acaggcgaca ggtactccgt
     2341 gaccccggat ggtaagtttt cttggttagg aaggaataca gactttatac agatgacaag
     2401 cggtgagact ctcgatccca ggcccatcga aagtttgctt tgcgagtcct cccttatatc
     2461 aagagcctgt gtaataggcg ataagttcct gaatgggccc gccacagcag tgtgtgccat
     2521 aatagaattg gagccgacta cggtagagaa aggtcaggcc catagcaggg acatagcccg
     2581 aatttttgcc cccataaacc gtgatttgcc tcctccttta agaatcgcct ggagccacgt
     2641 cctcgtgctc caacccagtg aaaaaatacc gatgaccaaa aaaggaacga tcttccgtaa
     2701 gaagatcgaa caagtctttg gtagtgcact tggtgggtcc tcaggtgaca attcccaagc
     2761 aaccactgat gcatcagtcg tccgtcgaga tgaactgtca aacactgtta agcatatcat
     2821 atcacgtgtt ctgggcgtga gcgacgacga gttgctgtgg actctctcat ttgccgaact
     2881 tggaatgacc tccgcccttg caacgagaat tgccaatgaa ttgaatgagg ttttagtagg
     2941 ggtcaatctg cctatcaatg catgttatat acacgttgac ttaccatccc tgtcaaatgc
     3001 agtatatgcc aaactggctc acttgaaact ccctgacaga acaccagagc cgcgtaaggc
     3061 accggttgag aatcctggcg gaaaagagat cgtaatagtg ggacaagcat ttagacttcc
     3121 aggctctatt aatgacgtgg ctagtctccg tgacgctttt ctggcccgtc aagcaagttc
     3181 aatcattaca gaaataccac cagacagatg ggatcacgca tccttctacc cgaaggacat
     3241 acgtttcaac aaggccggct tagtggacat tgctaactac gaccacagtt tcttcggctt
     3301 gacggctaca gaagctcttt acttatctcc gaccatgaga ctcgccctgg aagttagttt
     3361 tgaggccctt gagaacgcaa acattccggt ttctcagctg aaggggtcac aaacagctgt
     3421 ctacgttgct accacggatg acgggttcga gactctctta aatgcagagg ctgggtacga
     3481 cgcttacacc cgattctacg gtacggggcg tgcagcttcc acggcatccg gcaggatctc
     3541 atacctgctt gacgttcatg gacccagtat aactgtggac acggcatgta gtggaggggc
     3601 tgtttgcatt gatcaagcta tagactacct gcaatccagt tcagccgctg ataccgccat
     3661 catttgtgct tctaatactc attgctggcc aggatcattc atgtttctga gcgcccaggg
     3721 tatggtaagc tccggcggac gatgcgctac tttcacgacc gatgccgacg gctacgtacc
     3781 tagcgagggt gcagtcgcct tcattcttaa aacacgtgaa gcagctatga gggacaaaga
     3841 cacaattctg gctaccatca aagcaacaca aatttctcat aatggtaggt cccagggatt
     3901 agtcgccccc aatgtaaatt cccaagccga cttgcataga agcctgctcc aaaaagccgg
     3961 gctttcaccg gccgacattc atttcatcga ggcacacgga actggaacct ccctcggaga
     4021 tctgtccgag atccaagcta taaatgatgc atatacgagc tcacaacctc gaacggccgg
     4081 cccgctgatc gtttctgcaa gcaagacagt gataggacac actgaaccag ccggtccgtt
     4141 ggtggggatg ctgtcagtac tgaactcctt taaagagggg gccgtccctg gtttggcaca
     4201 cttgaccgcc gataacctga atcccgctct tgattgcagc agtgttccgt tactcatacc
     4261 ttaccagccc gttcacctgg cagcccccaa gcctcatcgt gctgctgtaa tgtcatatgg
     4321 attctctggc accctcggtg ggatcgtgct ggaggcacca gatgaggaaa gactcgaaga
     4381 ggaacctcct aatgacaaac ctatgttgtt tgtagttagt gcaaaaacac atactgcatt
     4441 aatagaatat ctcggccgat accttgaatt cctcttgcag gcaaatccac aagacttctg
     4501 cgatatttgt tatacttcat gcgttgggcg tgaacattat cgatacagat tcgcatgcgt
     4561 agccaacgac atggaggatt taataggaca attgcagaag cgtttaggct ctaaggtccc
     4621 accgaaacct tcctacaaaa gaggggccct tgcattcgcc ttcagcggtc agggcacaca
     4681 gttccgaggg atggctaccg agctcgcaaa agcctattcc ggattccgaa aaatagtctc
     4741 tgatctcgct aagagggcaa gtgaactctc aggtcacgct attgatcgat ttttgttagc
     4801 ttacgatatt ggggctgaaa acgtcgcccc ggatagcgag gctgatcaaa tatgtatctt
     4861 cgtatatcaa tgtagcgtgc ttcgttggct tcagacaatg ggcattagac catctgctgt
     4921 tatagggcac tccctcggtg agattagcgc ttctgtcgcc gccggtgcat tgtctctgga
     4981 ctctgcactg gaccttgtta tcagtcgagc tcgactgtta aggagctcta ccaatgcacc
     5041 cgccggtatg gcagccatgt cagcatcaca ggacgaagtt gttgagttga ttgggaaatt
     5101 agaccttgat aaagcaaatt cactctctgt ttctgttatc aatggtcctc agaatacggt
     5161 agtaagcggt tcttcagcag ccattgaaag tatagtcgca ctggccaaag ggcgaaagat
     5221 aaaagcaagt gctctcaaca tcaaccaagc cttccactct ccctacgtag attctgcagt
     5281 accaggtttg cgtgcttggt cagagaagca tatttcctct gctagaccat tacagatccc
     5341 cctgtactct acactccttg gcgcccaagt aagtgaggga caaatgctga atccggatca
     5401 ttgggtagat cacgcaagaa aacctgtcca attcgctcag gccgccacca ttatgaaaga
     5461 aagctttacc ggagtaatca ttgacatagg tcctcaagta gttgcttgga gtttgctgct
     5521 ctcaaacgga ctgacatcag ttaccgctct cgccgcaaag cgaggacgat cacagcaggt
     5581 tgccttcctt agtgctcttg cagatttata ccaagactat ggtgtcgtgc ctgactttgt
     5641 gggattatac gctcaacagg aggacgccag tcgtttaaag aaaaccgata ttctgaccta
     5701 tcctttccaa agggtgagaa ggtatccatc tttcatcccg agtcgtaggg cacctactca
     5761 tgcccatgtc caagatgagg aaacattgtc atctggatct tccacaccca cattggagaa
     5821 tacagatctc gactccggta aagagtcttt gatgggaccc acacgtggcc ttcttcgagt
     5881 agacgatctg agagacagta tcgtttcttc tgtaaaggat gtactggagc tgaaatcaaa
     5941 tgaggacttg gacttgtccg agtccttgaa cgcattggga atggacagta ttatgttcgc
     6001 tcaattacgt aaaaggatag gggagggact cgggttatcc gtcccgatgg tattcctgtc
     6061 tgacgccttc tcaatcggcg agatggttag taaccttgta gaacaggcag aggctagtga
     6121 ggacaactga gcttggaatg gatcttcgat cccgatcgtt caaacatttg gcaataaagt
     6181 ttcttaagat tgaatcctgt tgccggtctt gcgacgatta tcatataatt tctgttgaat
     6241 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     6301 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     6361 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attgccaagc
     6421 taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     6481 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6541 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6601 aatgcttcaa taatgggacc gactcgcgct gtcaggagac tagagccaag ctgatctcct
     6661 ttgccccgga gatcaccatg gacgactttc tctatctcta cgatctagga agaaagttcg
     6721 acggagaagg tgacgatacc atgttcacca ccgataatga gaagattagc ctcttcaatt
     6781 tcagaaagaa tgctgaccca cagatggtta gagaggccta cgcggcaggt ctgatcaaga
     6841 cgatctaccc gagtaataat ctccaggaga tcaaatacct tcccaagaag gttaaagatg
     6901 cagtcaaaag attcaggact aactgcatca agaacacaga gaaagatata tttctcaaga
     6961 tcagaagtac tattccagta tggacgattc aaggcttgct tcataaacca aggcaagtaa
     7021 tagagattgg agtctctaag aaagtagttc ctactgaatc aaaggccatg gagtcaaaaa
     7081 ttcagatcga ggatctaaca gaactcgccg tgaagactgg cgaacagttc atacagagtc
     7141 ttttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacactctcg
     7201 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
     7261 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
     7321 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
     7381 aggctatcgt tcaagatgcc cctgccgaca gtggtcccaa agatggaccc ccacccacga
     7441 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
     7501 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
     7561 ctatataagg aagttcattt catttggaga ggactccggt atttttacaa caattaccac
     7621 aacaaaacaa acaacaaaca acattacaat ttactattct agtcgaaatg gcatcctttg
     7681 aaaatagtct gtccgttttg atcgtcgggg ctggccttgg gggattagca gccgcaatag
     7741 ctcttaggag acagggccat gtagtaaaga tctatgatag ttcctcattc aaagctgaac
     7801 tgggtgctgg ccttgctgta cctccgaata cgcttagaag ccttcaacag ttaggttgta
     7861 atacagagaa tttgaacgga gtcgacaatc tctgcttcac ggccatgggt tacgacggct
     7921 ccgtaggtat gatgaataac atgacagact acagggaggc ttatggcaca tcctggatta
     7981 tggttcatcg agtggattta cataatgaac ttatgcgtgt cgcacttgat ccgggggggc
     8041 tgggaccacc agcaacatta catttaaatc acagagtgac cttctgcgac gttgacgcat
     8101 gtacggtaac ttttaccaac gggacgacac aaagtgccga cttgatcgta ggggctgacg
     8161 gcatacgaag caccatacgt agattcgtct tggaggaaga tgtaactgtg cctgccagcg
     8221 ggatcgtcgg tttcagatgg ctggtgcaag ccgatgcctt agacccttac cctgagttag
     8281 attggattgt taagaagcct ccactcggtg ctcgtctgat ctctacgcca cagaatccac
     8341 agtccggcgt ggggttggct gaccgtcgta caattatcat ctacgcttgt aggggtggca
     8401 cgatggttaa tgtgttggct gtccacgacg acgaaagaga tcagaatacg gcagattggt
     8461 cagtgccggc ttccaaagat gatctcttcc gtgtattcca tgactaccac ccaagattca
     8521 gacgtttgct cgaacttgct caagatatca atctctggca aatgagggta gttcccgtgc
     8581 ttaaaaaatg ggtgaacaag cgtgtctgtt tattaggaga tgccgcccat gcttctctcc
     8641 ccacgctggg tcaagggttc ggaatgggac tggaggacgc cgtggcactc ggcacattgt
     8701 tacccaaagg aactacggct tcacagatcg aaacgagact ggccgtttac gagcaattac
     8761 gaaaggatag ggctgagttc gtagcagcag aatcctacga ggaacagtat gtccctgaga
     8821 tgcgtgggct ctatcttcgt tccaaagaac tccgagatcg agtgatggga tatgatatta
     8881 aagtggagtc agaaaaggtt ttggagactc tgcttcgatc ctctaattct gcatgagctt
     8941 ggaatggatc ttcgatcccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     9001 tcctgttgcc ggtcttgcga cgattatcat ataatttctg ttgaattacg ttaagcatgt
     9061 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     9121 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     9181 atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg ccaagctaat tcttgaagac
     9241 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     9301 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9361 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9421 gggaccgact cgcgctgtca ggagactaga gccaagctga tctcctttgc cccggagatc
     9481 accatggacg actttctcta tctctacgat ctaggaagaa agttcgacgg agaaggtgac
     9541 gataccatgt tcaccaccga taatgagaag attagcctct tcaatttcag aaagaatgct
     9601 gacccacaga tggttagaga ggcctacgcg gcaggtctga tcaagacgat ctacccgagt
     9661 aataatctcc aggagatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc
     9721 aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt
     9781 ccagtatgga cgattcaagg cttgcttcat aaaccaaggc aagtaataga gattggagtc
     9841 tctaagaaag tagttcctac tgaatcaaag gccatggagt caaaaattca gatcgaggat
     9901 ctaacagaac tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat
     9961 gacaagaaga aaatcttcgt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
    10021 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
    10081 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
    10141 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
    10201 gatgcccctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
    10261 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
    10321 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
    10381 tcatttcatt tggagaggac tccggtattt ttacaacaat taccacaaca aaacaaacaa
    10441 caaacaacat tacaatttac tattctagtc gaaatggtga gcaagggcga ggagctgttc
    10501 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
    10561 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
    10621 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
    10681 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
    10741 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
    10801 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
    10861 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
    10921 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
    10981 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
    11041 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
    11101 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
    11161 atcactctcg gcatggacga gctgtacaag gacgagctgt aagcttggaa tggatcttcg
    11221 atcccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
    11281 ttgcgacgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
    11341 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
    11401 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
    11461 catctatgtt actagatcgg gaattgccaa gctaattctt gaagacgaaa gggcctcgtg
    11521 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    11581 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    11641 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatggga ccgactcgcg
    11701 ctgtcaggag actagagcca agctgatctc ctttgccccg gagatcacca tggacgactt
    11761 tctctatctc tacgatctag gaagaaagtt cgacggagaa ggtgacgata ccatgttcac
    11821 caccgataat gagaagatta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
    11881 tagagaggcc tacgcggcag gtctgatcaa gacgatctac ccgagtaata atctccagga
    11941 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat
    12001 caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat
    12061 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta agaaagtagt
    12121 tcctactgaa tcaaaggcca tggagtcaaa aattcagatc gaggatctaa cagaactcgc
    12181 cgtgaagact ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat
    12241 cttcgtcaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
    12301 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
    12361 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
    12421 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cccctgccga
    12481 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
    12541 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
    12601 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
    12661 gaggactccg gtatttttac aacaattacc acaacaaaac aaacaacaaa caacattaca
    12721 atttactatt ctagtcgaaa tggataatac tagcggacaa actttcgttt ctgtgaacct
    12781 ttctgacgaa agcaacacag cgaccactga cgtcaaaccc gtgagttcgg aagcggatcg
    12841 cttggatttt ttacagaaaa tgaatcccat tattatcgat gctttgatac ggaagaatag
    12901 ttatcagggc gctcgctttc gcgcgagaat aataggagtg tgcgtggatt gcggtagaaa
    12961 acacgataag gggttgaaga ctgaacgtaa gtgtaaggtc aacaatacgc agtctcagaa
    13021 cgaggtggcg catatgttaa tgcacgaccc cgttaagtat ttaaacaaaa gaaaagctag
    13081 agccttttct aatgcggaga tatttgcgat tgatttggtt atgtacacca aggaaaggca
    13141 attggctatt gatttggccg ctgaaaggga gaaaacgaga ctggctcgta gacacccgat
    13201 gcgttctccg gaagaaactc cggaatatta taaattcggt aggactgcta aagcaatgtt
    13261 accggacatc aacgccgtag acgttggtga taacgaggaa acttcgtcgg agtatccagt
    13321 gagtctgagt gtttctggcg gagttctccg cgaacaccac ttcatctgag cttggaatgg
    13381 atcttcgatc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
    13441 gccggtcttg cgacgattat catataattt ctgttgaatt acgttaagca tgtaataatt
    13501 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
    13561 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
    13621 gcggtgtcat ctatgttact agatcgggaa ttgccaagct aattcttgaa gacgaaaggg
    13681 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
    13741 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
    13801 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatgggaccg
    13861 actcgcgctg tcatgagacg aattctgaca ggatatattg gcgggtaaac ctaagagaaa
    13921 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
    13981 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
    14041 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtca tctgaaaacg
    14101 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
    14161 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
    14221 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
    14281 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
    14341 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
    14401 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
    14461 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
    14521 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
    14581 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
    14641 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
    14701 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
    14761 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
    14821 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
    14881 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
    14941 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
    15001 gcccgcgcac ctctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
    15061 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
    15121 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
    15181 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
    15241 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
    15301 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
    15361 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
    15421 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
    15481 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
    15541 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
    15601 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
    15661 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
    15721 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
    15781 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
    15841 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
    15901 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
    15961 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
    16021 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
    16081 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
    16141 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
    16201 ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag
    16261 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
    16321 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
    16381 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
    16441 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
    16501 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
    16561 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
    16621 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
    16681 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
    16741 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
    16801 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
    16861 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
    16921 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
    16981 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
    17041 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
    17101 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
    17161 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
    17221 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
    17281 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
    17341 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac
    17401 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
    17461 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
    17521 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
    17581 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
    17641 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
    17701 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
    17761 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
    17821 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg tgacggtcac agcttgtctg
    17881 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    17941 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
    18001 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    18061 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
    18121 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    18181 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    18241 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    18301 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    18361 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    18421 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    18481 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    18541 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    18601 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    18661 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
    18721 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    18781 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    18841 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    18901 ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtgatta gaaaaactca
    18961 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
    19021 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
    19081 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
    19141 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
    19201 aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
    19261 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt
    19321 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
    19381 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
    19441 tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
    19501 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
    19561 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
    19621 tcgggcttcc catacaatcg gtagattgtc gcacctgatt gcccgacatt atcgcgagcc
    19681 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac
    19741 gtttcccgtt gaatatggct cataacagaa cttattattt ccttcctctt ttctacagta
    19801 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
    19861 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
    19921 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
    19981 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
    20041 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
    20101 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
    20161 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
    20221 ataacgaatt cgtctca
//
