LOCUS       sequence-440579-        9066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the copper regulated expression of the PhiC31 recombinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4650
FEATURES             Location/Qualifiers
     source          1..9066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             359..2173
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             2174..2194
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2221..2473
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2508..2526)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2710..2734
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4035..4664
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5093..6166
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6232..6426
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6662..6684)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6770..6910
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6825..6844
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6925..6942)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7096..7684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7176..7195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7772..8587)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7772..8587)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8495..8514
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9020..9044
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagagtggt cttgggagag cgatgcgtct tttccgatga accgttccag caaaaaagac
       61 taagcgatgc gtcttttccg ctgaaccgtt ccagcaaaaa agactaagcg atgcgtcttt
      121 tccgctgaac cgttccagca aaaaagacta ggagagcgat gcgtcttttc cgctgaaccg
      181 ttccagcaaa aaagactatc ccgtcctaat acctctacgt gcaagcagta taaaaacaaa
      241 atacataaac ataactaaaa tctcatatcg ttgtaattgt catatactag tggtaattaa
      301 gtccagttac acttaaggtt aaatttgctg actctttttt acctttcatt tctgaaaaat
      361 ggacacgtat gccggcgctt atgatagaca atcaagagaa agagagaact cttctgccgc
      421 ctctccagct acacagagat ctgccaacga ggataaagct gctgatttac aaagagaggt
      481 agagagagat gggggtaggt ttagatttgt gggtcatttc tctgaggctc ctggtacatc
      541 agcctttggt actgctgaac ggcctgaatt tgaaagaata ttaaatgagt gtcgggccgg
      601 tcgactaaat atgataatag tttatgatgt atctaggttc agtagactca aagttatgga
      661 tgcaattccg atcgtgagcg agctacttgc actcggtgtt actatcgtaa gtacacaaga
      721 aggtgtattc aggcagggta atgtgatgga ccttatacac cttattatgc gactcgacgc
      781 tagccataaa gagtcatcac tgaagagtgc taaaatcttg gacaccaaga acctgcaaag
      841 ggagcttgga ggctatgtag gaggaaaagc tccgtatggt ttcgaactcg tgagtgagac
      901 aaaagagatt accagaaatg gtaggatggt gaacgtggtt attaataagc tagctcatag
      961 cacaacccct ctcacagggc cttttgagtt cgaacctgac gttattagat ggtggtggag
     1021 agagatcaaa actcacaaac atcttccatt taagccaggt agccaggctg ctatccaccc
     1081 tggatctatc actgggcttt gtaaaaggat ggatgctgac gcagtgccaa ctcgagggga
     1141 aacaatcggt aagaagactg catcttccgc ttgggatccc gctacagtta tgagaatcct
     1201 tcgggatccg cgtattgctg gattcgccgc agaagtcatt tataagaaaa aacctgatgg
     1261 tactcctact acaaaaatcg aaggttatag gatccaaagg gatcctatta ctttgcgtcc
     1321 agttgaatta gactgtggac cgatcatcga acctgccgag tggtacgagt tgcaggcctg
     1381 gcttgatggt agagggcgtg gaaagggttt atctcgggga caggcaattt tgtcagcaat
     1441 ggataagctt tattgtgagt gtggggctgt tatgacatca aaacgtggag aagagtcaat
     1501 caaggattct tacaggtgca gacgtcgtaa agtggtggac ccttctgctc caggacagca
     1561 tgagggtaca tgcaacgttt ctatggctgc tcttgataaa tttgtggccg aacgtatttt
     1621 caacaagatt agacacgctg aaggggatga ggaaacttta gctcttcttt gggaggctgc
     1681 ccggcgtttt ggtaagctaa cagaggcacc agaaaaaagt ggcgaaagag ctaatttggt
     1741 agccgaaaga gcagacgcct tgaatgcatt ggaagaactt tacgaagata gggctgccgg
     1801 agcatatgat ggaccagttg gtcggaagca tttcaggaag caacaagctg cactaacttt
     1861 gagacagcaa ggcgcagagg agcgactcgc agagcttgag gccgccgagg ccccaaagct
     1921 tccactcgat caatggtttc ctgaggatgc cgacgctgat cctacaggtc caaagagctg
     1981 gtggggaaga gctagcgtag acgataagag agtgtttgtg ggtctatttg ttgacaagat
     2041 tgtagttact aagtccacta cgggtcgagg acagggcact cccatcgaaa agagagccag
     2101 tattacctgg gcaaagcctc caaccgatga tgatgaagac gatgcacaag atggtaccga
     2161 ggatgtggca gcaccaaaaa aaaagaggaa ggtgtaagct tggaatggat cttcgatccc
     2221 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     2281 acgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     2341 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     2401 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     2461 atgttactag atcgggaatt gccaagctaa ttcttgaaga cgaaagggcc tcgtgatacg
     2521 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2581 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2641 tccgctcatg agacaataac cctgataaat gcttcaataa tgggaccgac tcgcgcttga
     2701 gacgaagctt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     2761 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     2821 ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag
     2881 tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt cgcacaagtc
     2941 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     3001 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     3061 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     3121 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     3181 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     3241 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     3301 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     3361 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     3421 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     3481 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     3541 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     3601 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     3661 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     3721 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     3781 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacctctca
     3841 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     3901 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     3961 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata
     4021 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     4081 cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     4141 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     4201 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     4261 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     4321 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     4381 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     4441 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     4501 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     4561 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     4621 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     4681 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     4741 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     4801 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     4861 cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga
     4921 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     4981 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     5041 ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg
     5101 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa
     5161 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga
     5221 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg
     5281 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat
     5341 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct
     5401 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt
     5461 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat
     5521 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc
     5581 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg
     5641 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc
     5701 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc
     5761 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat
     5821 cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac
     5881 ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc
     5941 acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag
     6001 tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa
     6061 tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat
     6121 gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat
     6181 gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggactctttc ctgtggatag
     6241 cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc
     6301 aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg
     6361 cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg
     6421 tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc
     6481 gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa
     6541 aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact
     6601 cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa
     6661 acctctgaca catgcagctc ccggtgacgg tcacagcttg tctgtaagcg gatgccggga
     6721 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     6781 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     6841 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     6901 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6961 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7021 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7081 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7141 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7201 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7261 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7321 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7381 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7441 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7501 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     7561 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7621 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7681 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7741 ttaagggatt ttggtcatgc attctaggtg attagaaaaa ctcatcgagc atcaaatgaa
     7801 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     7861 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     7921 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     7981 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     8041 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     8101 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagtcgaaat acgcgatcgc
     8161 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     8221 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     8281 ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     8341 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     8401 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     8461 atcggtagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     8521 aatcagcatc catgttggaa tttaatcgcg gccttgagca agacgtttcc cgttgaatat
     8581 ggctcataac agaacttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     8641 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     8701 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     8761 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     8821 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     8881 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
     8941 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
     9001 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacataaca agcttcgtct
     9061 cagtca
//
