LOCUS       sequence-440592-       24049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expression of the N. nambi HISPS gene under the 
            regulation of Solanum lycpersicum DFR promoter and TNOS and the 
            constitutve expression of CPH, LUZ, H3H, EGFP and p19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 24049)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 24049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4540
FEATURES             Location/Qualifiers
     source          1..24049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      6679..6931
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7937..8281
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     RBS             8409..8417
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      9299..9551
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        10557..10901
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(11271..11282)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      11802..12054
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        12898..13242
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      14608..14860
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        15704..16048
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(16122..16124,16125..16127,16128..16838)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(16122..16124,16125..16127,16128..16838)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(16167..16188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(16428..16447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     16775..16796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      16874..17126
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    17368..17392
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             18693..19322
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             19751..20824
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      20890..21084
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(21320..21342)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    21428..21568
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     21483..21502
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(21583..21600)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(21754..22342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(21834..21853)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(22430..23221)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    24007..24031
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagtgtttg aatggatcca gttcgtttta aagaaagaag gagaagtgat gaaaagttcg
       61 ttaaattata atataaaaaa atattgacgg attatagata attgtcatcc acttgacgga
      121 atcaatcacc acttccaagg acctgaatgc tgttatagtt tttttatcct tatataaatg
      181 gaatacatta tgaaattttt aaataaataa aaaaacacat tggtaatgtg aagaaaatgt
      241 agcaatatta tcgcactact acaacttgtc aaatattatt tgtcaagttt ctatccagga
      301 gtcttaatta acagcacatg gttaattttt cttctgaaag atttcatcag ctgttgaaat
      361 tttcttttct tttttaagaa cttgaaatct tgttaaagtc tatgatattc atcactattt
      421 ggatactttt ataggttaaa aaaaacacaa gttacatatt tgacatagga cgaaaatagt
      481 tatatccact aattaaatat acataattta tacactaatt atgtatagtt tgtgcatatt
      541 atatacctgc aagctgtttt gagtgaatag tgatgactat attaaaactg cgacaagcat
      601 tgcaaagtac tgaagtacat ataaacaaac atctctagat cgattcaaat tcaaatttgt
      661 aaacaggtaa agaaaatgtt tagcatgcct gcaaaacaaa aaggttcgtc cattttatac
      721 tttattttat taatttgatt ttctaacttt aactttatac gagctgaaaa aaagctaaaa
      781 atggctttaa atcttgtaaa cttaatgtac caaattgaaa ttaaaaagtt gtgtcaaaac
      841 agaaaagata tatccttttt taaaatagat taaaaatgaa agtatgataa acaaattgaa
      901 acatagagaa taattatttt taaatttcaa aagcattaac gtgaaattga ttcattatta
      961 actttagatg taatgagctt tcgaacaaaa taataacata ctcgatataa ttcaacaagc
     1021 taagtataga gaagatatat tgaatgcata caaactttgt attgagaagc tgtatctaat
     1081 agaatcttcg gcgtaaaaaa gtgaaatcaa ataccatact acatagtaat tctttattat
     1141 agggaaaaag aataaatata tcccaaacta ttaccatttt atccttaaaa aagaaaagat
     1201 ttcaatcgca tgtattcaat attaccattt acgatagttt taagatatat ttgtccttat
     1261 tccttttatt atatgtgctg ataagaaagt ggccagacac ttcacactag cccttcttct
     1321 ctcaccaacc agtcacattt ggttaatcca accaagttat tgaacggtat gttgagcacg
     1381 tgcttaccat ctaacattac cactcctaat acctctacgt gcaagcagta taaaaacaaa
     1441 atacataaac ataactaaaa tctcatatcg ttgtaattgt catatactag tggtaattaa
     1501 gtccagttac acttaaggtt aaatttgctg actctttttt acctttcatt tctgaaaaat
     1561 gaactcaagt aaaaatcctc cttctactct ccttgatgtt ttcttggata ccgcaagaaa
     1621 cttggataca gcaagcagaa atgtgctcga gtgtggtgaa catcgatgga gttacagaga
     1681 actggataca gtgtcctcag ctctcgccca gcatcttcga tacacggtgg ggctgtcccc
     1741 tactgtcgca gtgatcagcg agaatcaccc gtacatattg gccctcatgc tcgctgtatg
     1801 gaaattgggc ggtaccttcg ctccgataga tgtccacagc cctgcagaac tggttgccgg
     1861 gatgcttaac atagtctcac cgtcttgcct ggtcatcccg tcatcagacg taacaaacca
     1921 gacccttgca tgtgatctta acattccagt ggttgcattt catcctcacc aaagcacaat
     1981 cccggaactg aacaaaaagt acctgacgga cagtcaaatc agccctgatc tcccattccc
     2041 cgatcccaac cgtccggctc tttacctgtt cacttcttct gctacgagta gatccaactt
     2101 gaagtgcgtg cccctgaccc acaccttcat actgagaaat agcctgtcaa agagggcctg
     2161 gtgcaaaaga atgaggccag aaacagattt cgatggtata agggttttag gatgggcacc
     2221 atggagtcat gttctggctc acatgcaaga tatagggccc cttacactcc tgaatgctgg
     2281 atgttacgtc tttgcaacga caccgagcac atatccaacc gagttgaagg acgataggga
     2341 tgtcatttct tgcgccgcaa atgcagttat gtacaagggg gttaagtcct tcgcctgtct
     2401 cccgtttgtg ctgggaggcc ttaaggccct gtgtgagtcc gagccgtccg tcaaagcaca
     2461 attacaggtc gaggagcgag cacagttgtt aaagagctta caacacatga agattcttga
     2521 atgtggtggc gctatgctcg aagtttcagt ggcttcttgg gctatcgaga atcgtattcc
     2581 cattagtata ggaatcggta tgaccgaaac tggcggagcc ttgttcgctg gacccgttca
     2641 agcaatccaa acgggattta gttcagagga caagtttatt gaggatgcca catatctcct
     2701 tgtcaaagac gattacgagt cacacgcaga agaggacatc aatgaaggcg aactggttgt
     2761 taaaagccga atgctgccaa ggggttatct ggggtataac gatccatcct tctcagtgga
     2821 cgatgcaggg tgggttacct tcaaaacagg cgacaggtac tccgtgaccc cggatggtaa
     2881 gttttcttgg ttaggaagga atacagactt tatacagatg acaagcggtg agactctcga
     2941 tcccaggccc atcgaaagtt tgctttgcga gtcctccctt atatcaagag cctgtgtaat
     3001 aggcgataag ttcctgaatg ggcccgccac agcagtgtgt gccataatag aattggagcc
     3061 gactacggta gagaaaggtc aggcccatag cagggacata gcccgaattt ttgcccccat
     3121 aaaccgtgat ttgcctcctc ctttaagaat cgcctggagc cacgtcctcg tgctccaacc
     3181 cagtgaaaaa ataccgatga ccaaaaaagg aacgatcttc cgtaagaaga tcgaacaagt
     3241 ctttggtagt gcacttggtg ggtcctcagg tgacaattcc caagcaacca ctgatgcatc
     3301 agtcgtccgt cgagatgaac tgtcaaacac tgttaagcat atcatatcac gtgttctggg
     3361 cgtgagcgac gacgagttgc tgtggactct ctcatttgcc gaacttggaa tgacctccgc
     3421 ccttgcaacg agaattgcca atgaattgaa tgaggtttta gtaggggtca atctgcctat
     3481 caatgcatgt tatatacacg ttgacttacc atccctgtca aatgcagtat atgccaaact
     3541 ggctcacttg aaactccctg acagaacacc agagccgcgt aaggcaccgg ttgagaatcc
     3601 tggcggaaaa gagatcgtaa tagtgggaca agcatttaga cttccaggct ctattaatga
     3661 cgtggctagt ctccgtgacg cttttctggc ccgtcaagca agttcaatca ttacagaaat
     3721 accaccagac agatgggatc acgcatcctt ctacccgaag gacatacgtt tcaacaaggc
     3781 cggcttagtg gacattgcta actacgacca cagtttcttc ggcttgacgg ctacagaagc
     3841 tctttactta tctccgacca tgagactcgc cctggaagtt agttttgagg cccttgagaa
     3901 cgcaaacatt ccggtttctc agctgaaggg gtcacaaaca gctgtctacg ttgctaccac
     3961 ggatgacggg ttcgagactc tcttaaatgc agaggctggg tacgacgctt acacccgatt
     4021 ctacggtacg gggcgtgcag cttccacggc atccggcagg atctcatacc tgcttgacgt
     4081 tcatggaccc agtataactg tggacacggc atgtagtgga ggggctgttt gcattgatca
     4141 agctatagac tacctgcaat ccagttcagc cgctgatacc gccatcattt gtgcttctaa
     4201 tactcattgc tggccaggat cattcatgtt tctgagcgcc cagggtatgg taagctccgg
     4261 cggacgatgc gctactttca cgaccgatgc cgacggctac gtacctagcg agggtgcagt
     4321 cgccttcatt cttaaaacac gtgaagcagc tatgagggac aaagacacaa ttctggctac
     4381 catcaaagca acacaaattt ctcataatgg taggtcccag ggattagtcg cccccaatgt
     4441 aaattcccaa gccgacttgc atagaagcct gctccaaaaa gccgggcttt caccggccga
     4501 cattcatttc atcgaggcac acggaactgg aacctccctc ggagatctgt ccgagatcca
     4561 agctataaat gatgcatata cgagctcaca acctcgaacg gccggcccgc tgatcgtttc
     4621 tgcaagcaag acagtgatag gacacactga accagccggt ccgttggtgg ggatgctgtc
     4681 agtactgaac tcctttaaag agggggccgt ccctggtttg gcacacttga ccgccgataa
     4741 cctgaatccc gctcttgatt gcagcagtgt tccgttactc ataccttacc agcccgttca
     4801 cctggcagcc cccaagcctc atcgtgctgc tgtaatgtca tatggattct ctggcaccct
     4861 cggtgggatc gtgctggagg caccagatga ggaaagactc gaagaggaac ctcctaatga
     4921 caaacctatg ttgtttgtag ttagtgcaaa aacacatact gcattaatag aatatctcgg
     4981 ccgatacctt gaattcctct tgcaggcaaa tccacaagac ttctgcgata tttgttatac
     5041 ttcatgcgtt gggcgtgaac attatcgata cagattcgca tgcgtagcca acgacatgga
     5101 ggatttaata ggacaattgc agaagcgttt aggctctaag gtcccaccga aaccttccta
     5161 caaaagaggg gcccttgcat tcgccttcag cggtcagggc acacagttcc gagggatggc
     5221 taccgagctc gcaaaagcct attccggatt ccgaaaaata gtctctgatc tcgctaagag
     5281 ggcaagtgaa ctctcaggtc acgctattga tcgatttttg ttagcttacg atattggggc
     5341 tgaaaacgtc gccccggata gcgaggctga tcaaatatgt atcttcgtat atcaatgtag
     5401 cgtgcttcgt tggcttcaga caatgggcat tagaccatct gctgttatag ggcactccct
     5461 cggtgagatt agcgcttctg tcgccgccgg tgcattgtct ctggactctg cactggacct
     5521 tgttatcagt cgagctcgac tgttaaggag ctctaccaat gcacccgccg gtatggcagc
     5581 catgtcagca tcacaggacg aagttgttga gttgattggg aaattagacc ttgataaagc
     5641 aaattcactc tctgtttctg ttatcaatgg tcctcagaat acggtagtaa gcggttcttc
     5701 agcagccatt gaaagtatag tcgcactggc caaagggcga aagataaaag caagtgctct
     5761 caacatcaac caagccttcc actctcccta cgtagattct gcagtaccag gtttgcgtgc
     5821 ttggtcagag aagcatattt cctctgctag accattacag atccccctgt actctacact
     5881 ccttggcgcc caagtaagtg agggacaaat gctgaatccg gatcattggg tagatcacgc
     5941 aagaaaacct gtccaattcg ctcaggccgc caccattatg aaagaaagct ttaccggagt
     6001 aatcattgac ataggtcctc aagtagttgc ttggagtttg ctgctctcaa acggactgac
     6061 atcagttacc gctctcgccg caaagcgagg acgatcacag caggttgcct tccttagtgc
     6121 tcttgcagat ttataccaag actatggtgt cgtgcctgac tttgtgggat tatacgctca
     6181 acaggaggac gccagtcgtt taaagaaaac cgatattctg acctatcctt tccaaagggt
     6241 gagaaggtat ccatctttca tcccgagtcg tagggcacct actcatgccc atgtccaaga
     6301 tgaggaaaca ttgtcatctg gatcttccac acccacattg gagaatacag atctcgactc
     6361 cggtaaagag tctttgatgg gacccacacg tggccttctt cgagtagacg atctgagaga
     6421 cagtatcgtt tcttctgtaa aggatgtact ggagctgaaa tcaaatgagg acttggactt
     6481 gtccgagtcc ttgaacgcat tgggaatgga cagtattatg ttcgctcaat tacgtaaaag
     6541 gataggggag ggactcgggt tatccgtccc gatggtattc ctgtctgacg ccttctcaat
     6601 cggcgagatg gttagtaacc ttgtagaaca ggcagaggct agtgaggaca actgagcttg
     6661 gaatggatct tcgatcccga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     6721 cctgttgccg gtcttgcgac gattatcata taatttctgt tgaattacgt taagcatgta
     6781 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     6841 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     6901 tcgcgcgcgg tgtcatctat gttactagat cgggaattgc caagctaatt cttgaagacg
     6961 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     7021 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7081 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataatg
     7141 ggaccgactc gcgctgtcag gagtctcaat ggtaacttta ctctttattt aaccatacat
     7201 ttttttttat ttttttcact ttgttcttca tccactattg ttctttgttc atcttgaaca
     7261 aaagctccct ccttctttgt tcttcatcca ccattgttct tcatcaatca tttcgctgtc
     7321 aggagactag agccaagctg atctcctttg ccccggagat caccatggac gactttctct
     7381 atctctacga tctaggaaga aagttcgacg gagaaggtga cgataccatg ttcaccaccg
     7441 ataatgagaa gattagcctc ttcaatttca gaaagaatgc tgacccacag atggttagag
     7501 aggcctacgc ggcaggtctg atcaagacga tctacccgag taataatctc caggagatca
     7561 aataccttcc caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga
     7621 acacagagaa agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag
     7681 gcttgcttca taaaccaagg caagtaatag agattggagt ctctaagaaa gtagttccta
     7741 ctgaatcaaa ggccatggag tcaaaaattc agatcgagga tctaacagaa ctcgccgtga
     7801 agactggcga acagttcata cagagtcttt tacgactcaa tgacaagaag aaaatcttcg
     7861 tcaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
     7921 aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
     7981 tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct
     8041 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcccct gccgacagtg
     8101 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
     8161 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
     8221 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
     8281 ctccggtatt tttacaacaa ttaccacaac aaaacaaaca acaaacaaca ttacaattta
     8341 ctattctagt cgaaatggcc ccaataagtt ccacgtggtc taggctcata aggtttgtgg
     8401 ctgtggaaac ctccttagtt cacatcggcg agcctattga tgcaacaatg gacgtgggct
     8461 tagcacgtag ggaagggaag acaatacaag cctatgagat catcggcagt ggctctgctt
     8521 tagatctgtc tgcccaagtg tcaaagaacg tacttacggt ccgtgagctc ttaatgccct
     8581 tgagccgtga ggagattaaa acggtaagat gccttggcct taattatccc gtccacgcca
     8641 cagaagccaa cgtggccgtg ccgaaattcc cgaacctttt ttacaaaccc gtaacctccc
     8701 tcatcggacc aggtgggttg ataaccatcc cttcagttgt ccaaccacct aaagagcacc
     8761 agagcgatta cgaagccgag ttggttatcg tcatcggtaa agccgccaaa aatgtcagtg
     8821 aagatgaagc tctggactac gtgttgggct acaccgcagc caatgacatc tcatttagga
     8881 aacatcagtt ggccgtttct caatggtcat tctccaaggg ttttgatggc acgaatcccc
     8941 tgggaccgtg cctggtttct gcttcctcta ttccagatcc acaggatatt ccgatccaat
     9001 gtaaactcaa cggtggggtg gtgcaaaatg gaaacactcg tgatcagatt tttaatgtca
     9061 agaaaacaat ctctttttta agccaaggga ctacgttgga gcctggttcc ataatcctga
     9121 cggggacgcc cgacggagtg ggcttcgtaa gaaacccacc gctttatttg aaggacggtg
     9181 atgaagtaat gacttggatc ggatcaggta taggaacctt agccaacact gtccgagagg
     9241 agcagacttg tttcgcttca ggagggcacg agtaagcttg gaatggatct tcgatcccga
     9301 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgac
     9361 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     9421 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     9481 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     9541 gttactagat cgggaattgc caagctaatt cttgaagacg aaagggcctc gtgatacgcc
     9601 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     9661 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9721 cgctcatgag acaataaccc tgataaatgc ttcaataatg ggaccgactc gcgctgtcag
     9781 gagtctcaat ggtaacttta ctctttattt aaccatacat ttttttttat ttttttcact
     9841 ttgttcttca tccactattg ttctttgttc atcttgaaca aaagctccct ccttctttgt
     9901 tcttcatcca ccattgttct tcatcaatca tttcgctgtc aggagactag agccaagctg
     9961 atctcctttg ccccggagat caccatggac gactttctct atctctacga tctaggaaga
    10021 aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa gattagcctc
    10081 ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc ggcaggtctg
    10141 atcaagacga tctacccgag taataatctc caggagatca aataccttcc caagaaggtt
    10201 aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt
    10261 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg
    10321 caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa ggccatggag
    10381 tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga acagttcata
    10441 cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac
    10501 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
    10561 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
    10621 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
    10681 aaaggaaagg ctatcgttca agatgcccct gccgacagtg gtcccaaaga tggaccccca
    10741 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
    10801 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
    10861 ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa
    10921 ttaccacaac aaaacaaaca acaaacaaca ttacaattta ctattctagt cgaaatgaga
    10981 attaacatct ccctttcatc attatttgaa cgtctgtcaa agttatccag cagatccatt
    11041 gcaatcacct gtggtgtcgt cttggcctcc gcaatcgctt ttccgataat ccgtagggac
    11101 tatcagactt ttttggaagt cgggccgagt tacgctccgc agaatttcag aggttacatc
    11161 atagtatgcg tgctttcact ctttaggcag gaacaaaaag ggttggctat ctatgatcga
    11221 ctgcccgaaa agcgaaggtg gttggcagat ctcccattcc gagaaggtac gcgtccttct
    11281 atcacaagtc atatcatcca aagacagagg actcagctgg tagaccaaga atttgcaacc
    11341 cgagaactca ttgataaagt tatacccaga gttcaggctc gacacaccga taagacgttt
    11401 ctgtcaacct caaagttcga atttcatgcc aaagcaattt ttttgctgcc cagtattccc
    11461 atcaatgatc ccttgaatat accttcacat gatactgtca gacgaacaaa aagggagatc
    11521 gctcacatgc acgattacca cgactgcacc ctgcacttgg ctctcgcagc ccaggacggt
    11581 aaagaagtac ttaaaaaagg gtggggccag agacatcccc tcgctggccc cggcgtgccg
    11641 ggacctccaa ccgaatggac gtttttatat gcaccgcgta acgaggagga agccagagtg
    11701 gtcgagatga tagtggaggc cagtattggt tacatgacaa atgaccccgc tggtaaaatt
    11761 gtagagaacg ctaagtgagc ttggaatgga tcttcgatcc cgatcgttca aacatttggc
    11821 aataaagttt cttaagattg aatcctgttg ccggtcttgc gacgattatc atataatttc
    11881 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
    11941 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
    12001 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
    12061 tgccaagcta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
    12121 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
    12181 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
    12241 ccctgataaa tgcttcaata atgggaccga ctcgcgctgt caggagacta gagccaagct
    12301 gatctccttt gccccggaga tcaccatgga cgactttctc tatctctacg atctaggaag
    12361 aaagttcgac ggagaaggtg acgataccat gttcaccacc gataatgaga agattagcct
    12421 cttcaatttc agaaagaatg ctgacccaca gatggttaga gaggcctacg cggcaggtct
    12481 gatcaagacg atctacccga gtaataatct ccaggagatc aaataccttc ccaagaaggt
    12541 taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga aagatatatt
    12601 tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc ataaaccaag
    12661 gcaagtaata gagattggag tctctaagaa agtagttcct actgaatcaa aggccatgga
    12721 gtcaaaaatt cagatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat
    12781 acagagtctt ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga
    12841 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
    12901 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
    12961 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
    13021 taaaggaaag gctatcgttc aagatgcccc tgccgacagt ggtcccaaag atggaccccc
    13081 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
    13141 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
    13201 cccttcctct atataaggaa gttcatttca tttggagagg actccggtat ttttacaaca
    13261 attaccacaa caaaacaaac aacaaacaac attacaattt actattctag tcgaaatggc
    13321 atcctttgaa aatagtctgt ccgttttgat cgtcggggct ggccttgggg gattagcagc
    13381 cgcaatagct cttaggagac agggccatgt agtaaagatc tatgatagtt cctcattcaa
    13441 agctgaactg ggtgctggcc ttgctgtacc tccgaatacg cttagaagcc ttcaacagtt
    13501 aggttgtaat acagagaatt tgaacggagt cgacaatctc tgcttcacgg ccatgggtta
    13561 cgacggctcc gtaggtatga tgaataacat gacagactac agggaggctt atggcacatc
    13621 ctggattatg gttcatcgag tggatttaca taatgaactt atgcgtgtcg cacttgatcc
    13681 gggggggctg ggaccaccag caacattaca tttaaatcac agagtgacct tctgcgacgt
    13741 tgacgcatgt acggtaactt ttaccaacgg gacgacacaa agtgccgact tgatcgtagg
    13801 ggctgacggc atacgaagca ccatacgtag attcgtcttg gaggaagatg taactgtgcc
    13861 tgccagcggg atcgtcggtt tcagatggct ggtgcaagcc gatgccttag acccttaccc
    13921 tgagttagat tggattgtta agaagcctcc actcggtgct cgtctgatct ctacgccaca
    13981 gaatccacag tccggcgtgg ggttggctga ccgtcgtaca attatcatct acgcttgtag
    14041 gggtggcacg atggttaatg tgttggctgt ccacgacgac gaaagagatc agaatacggc
    14101 agattggtca gtgccggctt ccaaagatga tctcttccgt gtattccatg actaccaccc
    14161 aagattcaga cgtttgctcg aacttgctca agatatcaat ctctggcaaa tgagggtagt
    14221 tcccgtgctt aaaaaatggg tgaacaagcg tgtctgttta ttaggagatg ccgcccatgc
    14281 ttctctcccc acgctgggtc aagggttcgg aatgggactg gaggacgccg tggcactcgg
    14341 cacattgtta cccaaaggaa ctacggcttc acagatcgaa acgagactgg ccgtttacga
    14401 gcaattacga aaggataggg ctgagttcgt agcagcagaa tcctacgagg aacagtatgt
    14461 ccctgagatg cgtgggctct atcttcgttc caaagaactc cgagatcgag tgatgggata
    14521 tgatattaaa gtggagtcag aaaaggtttt ggagactctg cttcgatcct ctaattctgc
    14581 atgagcttgg aatggatctt cgatcccgat cgttcaaaca tttggcaata aagtttctta
    14641 agattgaatc ctgttgccgg tcttgcgacg attatcatat aatttctgtt gaattacgtt
    14701 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
    14761 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
    14821 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattgcc aagctaattc
    14881 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
    14941 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    15001 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    15061 tcaataatgg gaccgactcg cgctgtcagg agactagagc caagctgatc tcctttgccc
    15121 cggagatcac catggacgac tttctctatc tctacgatct aggaagaaag ttcgacggag
    15181 aaggtgacga taccatgttc accaccgata atgagaagat tagcctcttc aatttcagaa
    15241 agaatgctga cccacagatg gttagagagg cctacgcggc aggtctgatc aagacgatct
    15301 acccgagtaa taatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca
    15361 aaagattcag gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa
    15421 gtactattcc agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga
    15481 ttggagtctc taagaaagta gttcctactg aatcaaaggc catggagtca aaaattcaga
    15541 tcgaggatct aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac
    15601 gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacact ctcgtctact
    15661 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
    15721 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
    15781 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
    15841 tcgttcaaga tgcccctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
    15901 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
    15961 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
    16021 aaggaagttc atttcatttg gagaggactc cggtattttt acaacaatta ccacaacaaa
    16081 acaaacaaca aacaacatta caatttacta ttctagtcga aatggtgagc aagggcgagg
    16141 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
    16201 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
    16261 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
    16321 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
    16381 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
    16441 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
    16501 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
    16561 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
    16621 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
    16681 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
    16741 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
    16801 ccgccgggat cactctcggc atggacgagc tgtacaagga cgagctgtaa gcttggaatg
    16861 gatcttcgat cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
    16921 tgccggtctt gcgacgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
    16981 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
    17041 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
    17101 cgcggtgtca tctatgttac tagatcggga attgccaagc taattcttga agacgaaagg
    17161 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
    17221 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    17281 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatgggacc
    17341 gactcgcgct gtcatgagac cggatcctga caggatatat tggcgggtaa acctaagaga
    17401 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
    17461 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
    17521 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa
    17581 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
    17641 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
    17701 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
    17761 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
    17821 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
    17881 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
    17941 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
    18001 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
    18061 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
    18121 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
    18181 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
    18241 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
    18301 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
    18361 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
    18421 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
    18481 ccgcccgcgc acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
    18541 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
    18601 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
    18661 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
    18721 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
    18781 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
    18841 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
    18901 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
    18961 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
    19021 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
    19081 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
    19141 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
    19201 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
    19261 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
    19321 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
    19381 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
    19441 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
    19501 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
    19561 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
    19621 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
    19681 caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg
    19741 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
    19801 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
    19861 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
    19921 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
    19981 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
    20041 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
    20101 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
    20161 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
    20221 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
    20281 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
    20341 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
    20401 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
    20461 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
    20521 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
    20581 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
    20641 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
    20701 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
    20761 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
    20821 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
    20881 actctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
    20941 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
    21001 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
    21061 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
    21121 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
    21181 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
    21241 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
    21301 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc acagcttgtc
    21361 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    21421 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
    21481 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    21541 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
    21601 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    21661 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    21721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    21781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    21841 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    21901 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    21961 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    22021 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    22081 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    22141 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    22201 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    22261 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    22321 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    22381 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtgat tatttgccga
    22441 ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg
    22501 aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct
    22561 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt
    22621 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcac
    22681 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat
    22741 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat
    22801 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga
    22861 tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat
    22921 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct
    22981 cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg
    23041 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc
    23101 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
    23161 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca
    23221 taatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata
    23281 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
    23341 gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
    23401 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct
    23461 tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc cacggtgtgc
    23521 gtcacccggc aaccttgggt agcagcgaag tcgaggcatt tctgtcctgg ctggaacaga
    23581 acttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata
    23641 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag
    23701 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga
    23761 aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc
    23821 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg
    23881 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga
    23941 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct
    24001 ggctggtggc aggatatatt gtggtgtaaa cataacggat ccggtctca
//
