LOCUS       sequence-440597-       15756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expressino of the N. nambi HISPS gene under the P35S 
            promoter and the constitutive expression of EGFP and p19..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15756)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 15756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4808
FEATURES             Location/Qualifiers
     source          1..15756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        616..960
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      6154..6406
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7411..7755
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(7829..7831,7832..7834,7835..8545)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7829..7831,7832..7834,7835..8545)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7874..7895)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8135..8154)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8482..8503
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      8581..8833
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9075..9099
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10400..11029
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11458..12531
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12597..12791
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13027..13049)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13135..13275
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13190..13209
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13290..13307)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13461..14049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13541..13560)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14137..14928)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    15714..15738
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcccctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat tacaatttac
     1021 tattctagtc gaaatgaact caagtaaaaa tcctccttct actctccttg atgttttctt
     1081 ggataccgca agaaacttgg atacagcaag cagaaatgtg ctcgagtgtg gtgaacatcg
     1141 atggagttac agagaactgg atacagtgtc ctcagctctc gcccagcatc ttcgatacac
     1201 ggtggggctg tcccctactg tcgcagtgat cagcgagaat cacccgtaca tattggccct
     1261 catgctcgct gtatggaaat tgggcggtac cttcgctccg atagatgtcc acagccctgc
     1321 agaactggtt gccgggatgc ttaacatagt ctcaccgtct tgcctggtca tcccgtcatc
     1381 agacgtaaca aaccagaccc ttgcatgtga tcttaacatt ccagtggttg catttcatcc
     1441 tcaccaaagc acaatcccgg aactgaacaa aaagtacctg acggacagtc aaatcagccc
     1501 tgatctccca ttccccgatc ccaaccgtcc ggctctttac ctgttcactt cttctgctac
     1561 gagtagatcc aacttgaagt gcgtgcccct gacccacacc ttcatactga gaaatagcct
     1621 gtcaaagagg gcctggtgca aaagaatgag gccagaaaca gatttcgatg gtataagggt
     1681 tttaggatgg gcaccatgga gtcatgttct ggctcacatg caagatatag ggccccttac
     1741 actcctgaat gctggatgtt acgtctttgc aacgacaccg agcacatatc caaccgagtt
     1801 gaaggacgat agggatgtca tttcttgcgc cgcaaatgca gttatgtaca agggggttaa
     1861 gtccttcgcc tgtctcccgt ttgtgctggg aggccttaag gccctgtgtg agtccgagcc
     1921 gtccgtcaaa gcacaattac aggtcgagga gcgagcacag ttgttaaaga gcttacaaca
     1981 catgaagatt cttgaatgtg gtggcgctat gctcgaagtt tcagtggctt cttgggctat
     2041 cgagaatcgt attcccatta gtataggaat cggtatgacc gaaactggcg gagccttgtt
     2101 cgctggaccc gttcaagcaa tccaaacggg atttagttca gaggacaagt ttattgagga
     2161 tgccacatat ctccttgtca aagacgatta cgagtcacac gcagaagagg acatcaatga
     2221 aggcgaactg gttgttaaaa gccgaatgct gccaaggggt tatctggggt ataacgatcc
     2281 atccttctca gtggacgatg cagggtgggt taccttcaaa acaggcgaca ggtactccgt
     2341 gaccccggat ggtaagtttt cttggttagg aaggaataca gactttatac agatgacaag
     2401 cggtgagact ctcgatccca ggcccatcga aagtttgctt tgcgagtcct cccttatatc
     2461 aagagcctgt gtaataggcg ataagttcct gaatgggccc gccacagcag tgtgtgccat
     2521 aatagaattg gagccgacta cggtagagaa aggtcaggcc catagcaggg acatagcccg
     2581 aatttttgcc cccataaacc gtgatttgcc tcctccttta agaatcgcct ggagccacgt
     2641 cctcgtgctc caacccagtg aaaaaatacc gatgaccaaa aaaggaacga tcttccgtaa
     2701 gaagatcgaa caagtctttg gtagtgcact tggtgggtcc tcaggtgaca attcccaagc
     2761 aaccactgat gcatcagtcg tccgtcgaga tgaactgtca aacactgtta agcatatcat
     2821 atcacgtgtt ctgggcgtga gcgacgacga gttgctgtgg actctctcat ttgccgaact
     2881 tggaatgacc tccgcccttg caacgagaat tgccaatgaa ttgaatgagg ttttagtagg
     2941 ggtcaatctg cctatcaatg catgttatat acacgttgac ttaccatccc tgtcaaatgc
     3001 agtatatgcc aaactggctc acttgaaact ccctgacaga acaccagagc cgcgtaaggc
     3061 accggttgag aatcctggcg gaaaagagat cgtaatagtg ggacaagcat ttagacttcc
     3121 aggctctatt aatgacgtgg ctagtctccg tgacgctttt ctggcccgtc aagcaagttc
     3181 aatcattaca gaaataccac cagacagatg ggatcacgca tccttctacc cgaaggacat
     3241 acgtttcaac aaggccggct tagtggacat tgctaactac gaccacagtt tcttcggctt
     3301 gacggctaca gaagctcttt acttatctcc gaccatgaga ctcgccctgg aagttagttt
     3361 tgaggccctt gagaacgcaa acattccggt ttctcagctg aaggggtcac aaacagctgt
     3421 ctacgttgct accacggatg acgggttcga gactctctta aatgcagagg ctgggtacga
     3481 cgcttacacc cgattctacg gtacggggcg tgcagcttcc acggcatccg gcaggatctc
     3541 atacctgctt gacgttcatg gacccagtat aactgtggac acggcatgta gtggaggggc
     3601 tgtttgcatt gatcaagcta tagactacct gcaatccagt tcagccgctg ataccgccat
     3661 catttgtgct tctaatactc attgctggcc aggatcattc atgtttctga gcgcccaggg
     3721 tatggtaagc tccggcggac gatgcgctac tttcacgacc gatgccgacg gctacgtacc
     3781 tagcgagggt gcagtcgcct tcattcttaa aacacgtgaa gcagctatga gggacaaaga
     3841 cacaattctg gctaccatca aagcaacaca aatttctcat aatggtaggt cccagggatt
     3901 agtcgccccc aatgtaaatt cccaagccga cttgcataga agcctgctcc aaaaagccgg
     3961 gctttcaccg gccgacattc atttcatcga ggcacacgga actggaacct ccctcggaga
     4021 tctgtccgag atccaagcta taaatgatgc atatacgagc tcacaacctc gaacggccgg
     4081 cccgctgatc gtttctgcaa gcaagacagt gataggacac actgaaccag ccggtccgtt
     4141 ggtggggatg ctgtcagtac tgaactcctt taaagagggg gccgtccctg gtttggcaca
     4201 cttgaccgcc gataacctga atcccgctct tgattgcagc agtgttccgt tactcatacc
     4261 ttaccagccc gttcacctgg cagcccccaa gcctcatcgt gctgctgtaa tgtcatatgg
     4321 attctctggc accctcggtg ggatcgtgct ggaggcacca gatgaggaaa gactcgaaga
     4381 ggaacctcct aatgacaaac ctatgttgtt tgtagttagt gcaaaaacac atactgcatt
     4441 aatagaatat ctcggccgat accttgaatt cctcttgcag gcaaatccac aagacttctg
     4501 cgatatttgt tatacttcat gcgttgggcg tgaacattat cgatacagat tcgcatgcgt
     4561 agccaacgac atggaggatt taataggaca attgcagaag cgtttaggct ctaaggtccc
     4621 accgaaacct tcctacaaaa gaggggccct tgcattcgcc ttcagcggtc agggcacaca
     4681 gttccgaggg atggctaccg agctcgcaaa agcctattcc ggattccgaa aaatagtctc
     4741 tgatctcgct aagagggcaa gtgaactctc aggtcacgct attgatcgat ttttgttagc
     4801 ttacgatatt ggggctgaaa acgtcgcccc ggatagcgag gctgatcaaa tatgtatctt
     4861 cgtatatcaa tgtagcgtgc ttcgttggct tcagacaatg ggcattagac catctgctgt
     4921 tatagggcac tccctcggtg agattagcgc ttctgtcgcc gccggtgcat tgtctctgga
     4981 ctctgcactg gaccttgtta tcagtcgagc tcgactgtta aggagctcta ccaatgcacc
     5041 cgccggtatg gcagccatgt cagcatcaca ggacgaagtt gttgagttga ttgggaaatt
     5101 agaccttgat aaagcaaatt cactctctgt ttctgttatc aatggtcctc agaatacggt
     5161 agtaagcggt tcttcagcag ccattgaaag tatagtcgca ctggccaaag ggcgaaagat
     5221 aaaagcaagt gctctcaaca tcaaccaagc cttccactct ccctacgtag attctgcagt
     5281 accaggtttg cgtgcttggt cagagaagca tatttcctct gctagaccat tacagatccc
     5341 cctgtactct acactccttg gcgcccaagt aagtgaggga caaatgctga atccggatca
     5401 ttgggtagat cacgcaagaa aacctgtcca attcgctcag gccgccacca ttatgaaaga
     5461 aagctttacc ggagtaatca ttgacatagg tcctcaagta gttgcttgga gtttgctgct
     5521 ctcaaacgga ctgacatcag ttaccgctct cgccgcaaag cgaggacgat cacagcaggt
     5581 tgccttcctt agtgctcttg cagatttata ccaagactat ggtgtcgtgc ctgactttgt
     5641 gggattatac gctcaacagg aggacgccag tcgtttaaag aaaaccgata ttctgaccta
     5701 tcctttccaa agggtgagaa ggtatccatc tttcatcccg agtcgtaggg cacctactca
     5761 tgcccatgtc caagatgagg aaacattgtc atctggatct tccacaccca cattggagaa
     5821 tacagatctc gactccggta aagagtcttt gatgggaccc acacgtggcc ttcttcgagt
     5881 agacgatctg agagacagta tcgtttcttc tgtaaaggat gtactggagc tgaaatcaaa
     5941 tgaggacttg gacttgtccg agtccttgaa cgcattggga atggacagta ttatgttcgc
     6001 tcaattacgt aaaaggatag gggagggact cgggttatcc gtcccgatgg tattcctgtc
     6061 tgacgccttc tcaatcggcg agatggttag taaccttgta gaacaggcag aggctagtga
     6121 ggacaactga gcttggaatg gatcttcgat cccgatcgtt caaacatttg gcaataaagt
     6181 ttcttaagat tgaatcctgt tgccggtctt gcgacgatta tcatataatt tctgttgaat
     6241 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     6301 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     6361 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attgccaagc
     6421 taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     6481 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6541 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6601 aatgcttcaa taatgggacc gactcgcgct gtcaggagct caatggtaac tttactcttt
     6661 atttaaccat acattttttt ttattttttt cactttgttc ttcatccact attgttcttt
     6721 gttcatcttg aacaaaagct ccctccttct ttgttcttca tccaccattg ttcttcatca
     6781 atcatttcgc tgtcaggaga ctagagccaa gctgatctcc tttgccccgg agatcaccat
     6841 ggacgacttt ctctatctct acgatctagg aagaaagttc gacggagaag gtgacgatac
     6901 catgttcacc accgataatg agaagattag cctcttcaat ttcagaaaga atgctgaccc
     6961 acagatggtt agagaggcct acgcggcagg tctgatcaag acgatctacc cgagtaataa
     7021 tctccaggag atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac
     7081 taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt
     7141 atggacgatt caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa
     7201 gaaagtagtt cctactgaat caaaggccat ggagtcaaaa attcagatcg aggatctaac
     7261 agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa
     7321 gaagaaaatc ttcgtcaaca tggtggagca cgacactctc gtctactcca agaatatcaa
     7381 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg
     7441 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
     7501 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
     7561 ccctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     7621 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     7681 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     7741 tcatttggag aggactccgg tatttttaca acaattacca caacaaaaca aacaacaaac
     7801 aacattacaa tttactattc tagtcgaaat ggtgagcaag ggcgaggagc tgttcaccgg
     7861 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     7921 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     7981 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     8041 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     8101 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     8161 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     8221 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     8281 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     8341 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     8401 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     8461 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     8521 tctcggcatg gacgagctgt acaaggacga gctgtaagct tggaatggat cttcgatccc
     8581 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     8641 acgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     8701 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     8761 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     8821 atgttactag atcgggaatt gccaagctaa ttcttgaaga cgaaagggcc tcgtgatacg
     8881 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     8941 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     9001 tccgctcatg agacaataac cctgataaat gcttcaataa tgggaccgac tcgcgctgtc
     9061 atgagaccgg atcctgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     9121 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     9181 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc
     9241 tatagtgcag tcggcttctg acgttcagtg cagccgtcat ctgaaaacga catgtcgcac
     9301 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
     9361 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
     9421 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
     9481 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
     9541 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
     9601 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
     9661 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
     9721 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
     9781 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc
     9841 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
     9901 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
     9961 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
    10021 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
    10081 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
    10141 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacc
    10201 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct
    10261 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
    10321 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
    10381 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
    10441 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
    10501 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
    10561 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
    10621 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
    10681 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
    10741 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
    10801 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
    10861 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
    10921 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
    10981 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
    11041 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
    11101 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
    11161 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
    11221 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
    11281 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
    11341 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
    11401 ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
    11461 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
    11521 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
    11581 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
    11641 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
    11701 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
    11761 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
    11821 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
    11881 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
    11941 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
    12001 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
    12061 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
    12121 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
    12181 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
    12241 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
    12301 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
    12361 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
    12421 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
    12481 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
    12541 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggact ctttcctgtg
    12601 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
    12661 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
    12721 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
    12781 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
    12841 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
    12901 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
    12961 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
    13021 tgaaaacctc tgacacatgc agctcccggt gacggtcaca gcttgtctgt aagcggatgc
    13081 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
    13141 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
    13201 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    13261 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    13321 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    13381 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    13441 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    13501 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    13561 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    13621 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    13681 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    13741 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    13801 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    13861 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    13921 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    13981 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    14041 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    14101 tcacgttaag ggattttggt catgcattct aggtgattat ttgccgacta ccttggtgat
    14161 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
    14221 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
    14281 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
    14341 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcaccag cccagtcggg
    14401 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
    14461 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
    14521 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
    14581 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
    14641 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
    14701 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
    14761 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
    14821 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
    14881 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
    14941 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
    15001 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
    15061 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
    15121 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
    15181 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
    15241 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gaacagaact tattatttcc
    15301 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
    15361 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
    15421 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc
    15481 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
    15541 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
    15601 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
    15661 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
    15721 atatattgtg gtgtaaacat aacggatccg gtctca
//
