LOCUS       sequence-440598-       22848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expression of the N. nambi HISPS gene under the 
            regulation of the copper inducible CBS4x:mDFR promoter and TNOS and 
            the constitutve expression of CPH, LUZ, H3H, EGFP and p19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22848)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 22848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4542
FEATURES             Location/Qualifiers
     source          1..22848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5478..5730
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        6736..7080
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     RBS             7208..7216
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      8098..8350
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        9356..9700
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(10070..10081)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      10601..10853
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        11697..12041
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      13407..13659
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        14503..14847
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(14921..14923,14924..14926,14927..15637)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(14921..14923,14924..14926,14927..15637)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(14966..14987)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(15227..15246)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     15574..15595
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      15673..15925
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    16167..16191
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             17492..18121
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             18550..19623
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      19689..19883
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(20119..20141)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    20227..20367
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     20282..20301
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(20382..20399)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(20553..21141)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(20633..20652)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(21229..22020)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    22806..22830
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagagtggt cttgggagag cgatgcgtct tttccgatga accgttccag caaaaaagac
       61 taagcgatgc gtcttttccg ctgaaccgtt ccagcaaaaa agactaagcg atgcgtcttt
      121 tccgctgaac cgttccagca aaaaagacta ggagagcgat gcgtcttttc cgctgaaccg
      181 ttccagcaaa aaagactatc ccgtcctaat acctctacgt gcaagcagta taaaaacaaa
      241 atacataaac ataactaaaa tctcatatcg ttgtaattgt catatactag tggtaattaa
      301 gtccagttac acttaaggtt aaatttgctg actctttttt acctttcatt tctgaaaatg
      361 aactcaagta aaaatcctcc ttctactctc cttgatgttt tcttggatac cgcaagaaac
      421 ttggatacag caagcagaaa tgtgctcgag tgtggtgaac atcgatggag ttacagagaa
      481 ctggatacag tgtcctcagc tctcgcccag catcttcgat acacggtggg gctgtcccct
      541 actgtcgcag tgatcagcga gaatcacccg tacatattgg ccctcatgct cgctgtatgg
      601 aaattgggcg gtaccttcgc tccgatagat gtccacagcc ctgcagaact ggttgccggg
      661 atgcttaaca tagtctcacc gtcttgcctg gtcatcccgt catcagacgt aacaaaccag
      721 acccttgcat gtgatcttaa cattccagtg gttgcatttc atcctcacca aagcacaatc
      781 ccggaactga acaaaaagta cctgacggac agtcaaatca gccctgatct cccattcccc
      841 gatcccaacc gtccggctct ttacctgttc acttcttctg ctacgagtag atccaacttg
      901 aagtgcgtgc ccctgaccca caccttcata ctgagaaata gcctgtcaaa gagggcctgg
      961 tgcaaaagaa tgaggccaga aacagatttc gatggtataa gggttttagg atgggcacca
     1021 tggagtcatg ttctggctca catgcaagat atagggcccc ttacactcct gaatgctgga
     1081 tgttacgtct ttgcaacgac accgagcaca tatccaaccg agttgaagga cgatagggat
     1141 gtcatttctt gcgccgcaaa tgcagttatg tacaaggggg ttaagtcctt cgcctgtctc
     1201 ccgtttgtgc tgggaggcct taaggccctg tgtgagtccg agccgtccgt caaagcacaa
     1261 ttacaggtcg aggagcgagc acagttgtta aagagcttac aacacatgaa gattcttgaa
     1321 tgtggtggcg ctatgctcga agtttcagtg gcttcttggg ctatcgagaa tcgtattccc
     1381 attagtatag gaatcggtat gaccgaaact ggcggagcct tgttcgctgg acccgttcaa
     1441 gcaatccaaa cgggatttag ttcagaggac aagtttattg aggatgccac atatctcctt
     1501 gtcaaagacg attacgagtc acacgcagaa gaggacatca atgaaggcga actggttgtt
     1561 aaaagccgaa tgctgccaag gggttatctg gggtataacg atccatcctt ctcagtggac
     1621 gatgcagggt gggttacctt caaaacaggc gacaggtact ccgtgacccc ggatggtaag
     1681 ttttcttggt taggaaggaa tacagacttt atacagatga caagcggtga gactctcgat
     1741 cccaggccca tcgaaagttt gctttgcgag tcctccctta tatcaagagc ctgtgtaata
     1801 ggcgataagt tcctgaatgg gcccgccaca gcagtgtgtg ccataataga attggagccg
     1861 actacggtag agaaaggtca ggcccatagc agggacatag cccgaatttt tgcccccata
     1921 aaccgtgatt tgcctcctcc tttaagaatc gcctggagcc acgtcctcgt gctccaaccc
     1981 agtgaaaaaa taccgatgac caaaaaagga acgatcttcc gtaagaagat cgaacaagtc
     2041 tttggtagtg cacttggtgg gtcctcaggt gacaattccc aagcaaccac tgatgcatca
     2101 gtcgtccgtc gagatgaact gtcaaacact gttaagcata tcatatcacg tgttctgggc
     2161 gtgagcgacg acgagttgct gtggactctc tcatttgccg aacttggaat gacctccgcc
     2221 cttgcaacga gaattgccaa tgaattgaat gaggttttag taggggtcaa tctgcctatc
     2281 aatgcatgtt atatacacgt tgacttacca tccctgtcaa atgcagtata tgccaaactg
     2341 gctcacttga aactccctga cagaacacca gagccgcgta aggcaccggt tgagaatcct
     2401 ggcggaaaag agatcgtaat agtgggacaa gcatttagac ttccaggctc tattaatgac
     2461 gtggctagtc tccgtgacgc ttttctggcc cgtcaagcaa gttcaatcat tacagaaata
     2521 ccaccagaca gatgggatca cgcatccttc tacccgaagg acatacgttt caacaaggcc
     2581 ggcttagtgg acattgctaa ctacgaccac agtttcttcg gcttgacggc tacagaagct
     2641 ctttacttat ctccgaccat gagactcgcc ctggaagtta gttttgaggc ccttgagaac
     2701 gcaaacattc cggtttctca gctgaagggg tcacaaacag ctgtctacgt tgctaccacg
     2761 gatgacgggt tcgagactct cttaaatgca gaggctgggt acgacgctta cacccgattc
     2821 tacggtacgg ggcgtgcagc ttccacggca tccggcagga tctcatacct gcttgacgtt
     2881 catggaccca gtataactgt ggacacggca tgtagtggag gggctgtttg cattgatcaa
     2941 gctatagact acctgcaatc cagttcagcc gctgataccg ccatcatttg tgcttctaat
     3001 actcattgct ggccaggatc attcatgttt ctgagcgccc agggtatggt aagctccggc
     3061 ggacgatgcg ctactttcac gaccgatgcc gacggctacg tacctagcga gggtgcagtc
     3121 gccttcattc ttaaaacacg tgaagcagct atgagggaca aagacacaat tctggctacc
     3181 atcaaagcaa cacaaatttc tcataatggt aggtcccagg gattagtcgc ccccaatgta
     3241 aattcccaag ccgacttgca tagaagcctg ctccaaaaag ccgggctttc accggccgac
     3301 attcatttca tcgaggcaca cggaactgga acctccctcg gagatctgtc cgagatccaa
     3361 gctataaatg atgcatatac gagctcacaa cctcgaacgg ccggcccgct gatcgtttct
     3421 gcaagcaaga cagtgatagg acacactgaa ccagccggtc cgttggtggg gatgctgtca
     3481 gtactgaact cctttaaaga gggggccgtc cctggtttgg cacacttgac cgccgataac
     3541 ctgaatcccg ctcttgattg cagcagtgtt ccgttactca taccttacca gcccgttcac
     3601 ctggcagccc ccaagcctca tcgtgctgct gtaatgtcat atggattctc tggcaccctc
     3661 ggtgggatcg tgctggaggc accagatgag gaaagactcg aagaggaacc tcctaatgac
     3721 aaacctatgt tgtttgtagt tagtgcaaaa acacatactg cattaataga atatctcggc
     3781 cgataccttg aattcctctt gcaggcaaat ccacaagact tctgcgatat ttgttatact
     3841 tcatgcgttg ggcgtgaaca ttatcgatac agattcgcat gcgtagccaa cgacatggag
     3901 gatttaatag gacaattgca gaagcgttta ggctctaagg tcccaccgaa accttcctac
     3961 aaaagagggg cccttgcatt cgccttcagc ggtcagggca cacagttccg agggatggct
     4021 accgagctcg caaaagccta ttccggattc cgaaaaatag tctctgatct cgctaagagg
     4081 gcaagtgaac tctcaggtca cgctattgat cgatttttgt tagcttacga tattggggct
     4141 gaaaacgtcg ccccggatag cgaggctgat caaatatgta tcttcgtata tcaatgtagc
     4201 gtgcttcgtt ggcttcagac aatgggcatt agaccatctg ctgttatagg gcactccctc
     4261 ggtgagatta gcgcttctgt cgccgccggt gcattgtctc tggactctgc actggacctt
     4321 gttatcagtc gagctcgact gttaaggagc tctaccaatg cacccgccgg tatggcagcc
     4381 atgtcagcat cacaggacga agttgttgag ttgattggga aattagacct tgataaagca
     4441 aattcactct ctgtttctgt tatcaatggt cctcagaata cggtagtaag cggttcttca
     4501 gcagccattg aaagtatagt cgcactggcc aaagggcgaa agataaaagc aagtgctctc
     4561 aacatcaacc aagccttcca ctctccctac gtagattctg cagtaccagg tttgcgtgct
     4621 tggtcagaga agcatatttc ctctgctaga ccattacaga tccccctgta ctctacactc
     4681 cttggcgccc aagtaagtga gggacaaatg ctgaatccgg atcattgggt agatcacgca
     4741 agaaaacctg tccaattcgc tcaggccgcc accattatga aagaaagctt taccggagta
     4801 atcattgaca taggtcctca agtagttgct tggagtttgc tgctctcaaa cggactgaca
     4861 tcagttaccg ctctcgccgc aaagcgagga cgatcacagc aggttgcctt ccttagtgct
     4921 cttgcagatt tataccaaga ctatggtgtc gtgcctgact ttgtgggatt atacgctcaa
     4981 caggaggacg ccagtcgttt aaagaaaacc gatattctga cctatccttt ccaaagggtg
     5041 agaaggtatc catctttcat cccgagtcgt agggcaccta ctcatgccca tgtccaagat
     5101 gaggaaacat tgtcatctgg atcttccaca cccacattgg agaatacaga tctcgactcc
     5161 ggtaaagagt ctttgatggg acccacacgt ggccttcttc gagtagacga tctgagagac
     5221 agtatcgttt cttctgtaaa ggatgtactg gagctgaaat caaatgagga cttggacttg
     5281 tccgagtcct tgaacgcatt gggaatggac agtattatgt tcgctcaatt acgtaaaagg
     5341 ataggggagg gactcgggtt atccgtcccg atggtattcc tgtctgacgc cttctcaatc
     5401 ggcgagatgg ttagtaacct tgtagaacag gcagaggcta gtgaggacaa ctgagcttgg
     5461 aatggatctt cgatcccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     5521 ctgttgccgg tcttgcgacg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     5581 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     5641 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     5701 cgcgcgcggt gtcatctatg ttactagatc gggaattgcc aagctaattc ttgaagacga
     5761 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5821 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5881 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatgg
     5941 gaccgactcg cgctgtcagg agtctcaatg gtaactttac tctttattta accatacatt
     6001 tttttttatt tttttcactt tgttcttcat ccactattgt tctttgttca tcttgaacaa
     6061 aagctccctc cttctttgtt cttcatccac cattgttctt catcaatcat ttcgctgtca
     6121 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
     6181 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
     6241 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
     6301 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
     6361 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
     6421 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
     6481 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
     6541 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
     6601 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
     6661 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
     6721 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
     6781 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
     6841 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcccctg ccgacagtgg
     6901 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
     6961 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
     7021 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
     7081 tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat tacaatttac
     7141 tattctagtc gaaatggccc caataagttc cacgtggtct aggctcataa ggtttgtggc
     7201 tgtggaaacc tccttagttc acatcggcga gcctattgat gcaacaatgg acgtgggctt
     7261 agcacgtagg gaagggaaga caatacaagc ctatgagatc atcggcagtg gctctgcttt
     7321 agatctgtct gcccaagtgt caaagaacgt acttacggtc cgtgagctct taatgccctt
     7381 gagccgtgag gagattaaaa cggtaagatg ccttggcctt aattatcccg tccacgccac
     7441 agaagccaac gtggccgtgc cgaaattccc gaaccttttt tacaaacccg taacctccct
     7501 catcggacca ggtgggttga taaccatccc ttcagttgtc caaccaccta aagagcacca
     7561 gagcgattac gaagccgagt tggttatcgt catcggtaaa gccgccaaaa atgtcagtga
     7621 agatgaagct ctggactacg tgttgggcta caccgcagcc aatgacatct catttaggaa
     7681 acatcagttg gccgtttctc aatggtcatt ctccaagggt tttgatggca cgaatcccct
     7741 gggaccgtgc ctggtttctg cttcctctat tccagatcca caggatattc cgatccaatg
     7801 taaactcaac ggtggggtgg tgcaaaatgg aaacactcgt gatcagattt ttaatgtcaa
     7861 gaaaacaatc tcttttttaa gccaagggac tacgttggag cctggttcca taatcctgac
     7921 ggggacgccc gacggagtgg gcttcgtaag aaacccaccg ctttatttga aggacggtga
     7981 tgaagtaatg acttggatcg gatcaggtat aggaacctta gccaacactg tccgagagga
     8041 gcagacttgt ttcgcttcag gagggcacga gtaagcttgg aatggatctt cgatcccgat
     8101 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgacg
     8161 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     8221 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     8281 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     8341 ttactagatc gggaattgcc aagctaattc ttgaagacga aagggcctcg tgatacgcct
     8401 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     8461 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8521 gctcatgaga caataaccct gataaatgct tcaataatgg gaccgactcg cgctgtcagg
     8581 agtctcaatg gtaactttac tctttattta accatacatt tttttttatt tttttcactt
     8641 tgttcttcat ccactattgt tctttgttca tcttgaacaa aagctccctc cttctttgtt
     8701 cttcatccac cattgttctt catcaatcat ttcgctgtca ggagactaga gccaagctga
     8761 tctcctttgc cccggagatc accatggacg actttctcta tctctacgat ctaggaagaa
     8821 agttcgacgg agaaggtgac gataccatgt tcaccaccga taatgagaag attagcctct
     8881 tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgcg gcaggtctga
     8941 tcaagacgat ctacccgagt aataatctcc aggagatcaa ataccttccc aagaaggtta
     9001 aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc
     9061 tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat aaaccaaggc
     9121 aagtaataga gattggagtc tctaagaaag tagttcctac tgaatcaaag gccatggagt
     9181 caaaaattca gatcgaggat ctaacagaac tcgccgtgaa gactggcgaa cagttcatac
     9241 agagtctttt acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca
     9301 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga
     9361 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     9421 acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata
     9481 aaggaaaggc tatcgttcaa gatgcccctg ccgacagtgg tcccaaagat ggacccccac
     9541 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     9601 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     9661 cttcctctat ataaggaagt tcatttcatt tggagaggac tccggtattt ttacaacaat
     9721 taccacaaca aaacaaacaa caaacaacat tacaatttac tattctagtc gaaatgagaa
     9781 ttaacatctc cctttcatca ttatttgaac gtctgtcaaa gttatccagc agatccattg
     9841 caatcacctg tggtgtcgtc ttggcctccg caatcgcttt tccgataatc cgtagggact
     9901 atcagacttt tttggaagtc gggccgagtt acgctccgca gaatttcaga ggttacatca
     9961 tagtatgcgt gctttcactc tttaggcagg aacaaaaagg gttggctatc tatgatcgac
    10021 tgcccgaaaa gcgaaggtgg ttggcagatc tcccattccg agaaggtacg cgtccttcta
    10081 tcacaagtca tatcatccaa agacagagga ctcagctggt agaccaagaa tttgcaaccc
    10141 gagaactcat tgataaagtt atacccagag ttcaggctcg acacaccgat aagacgtttc
    10201 tgtcaacctc aaagttcgaa tttcatgcca aagcaatttt tttgctgccc agtattccca
    10261 tcaatgatcc cttgaatata ccttcacatg atactgtcag acgaacaaaa agggagatcg
    10321 ctcacatgca cgattaccac gactgcaccc tgcacttggc tctcgcagcc caggacggta
    10381 aagaagtact taaaaaaggg tggggccaga gacatcccct cgctggcccc ggcgtgccgg
    10441 gacctccaac cgaatggacg tttttatatg caccgcgtaa cgaggaggaa gccagagtgg
    10501 tcgagatgat agtggaggcc agtattggtt acatgacaaa tgaccccgct ggtaaaattg
    10561 tagagaacgc taagtgagct tggaatggat cttcgatccc gatcgttcaa acatttggca
    10621 ataaagtttc ttaagattga atcctgttgc cggtcttgcg acgattatca tataatttct
    10681 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
    10741 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
    10801 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
    10861 gccaagctaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
    10921 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
    10981 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    11041 cctgataaat gcttcaataa tgggaccgac tcgcgctgtc aggagactag agccaagctg
    11101 atctcctttg ccccggagat caccatggac gactttctct atctctacga tctaggaaga
    11161 aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa gattagcctc
    11221 ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc ggcaggtctg
    11281 atcaagacga tctacccgag taataatctc caggagatca aataccttcc caagaaggtt
    11341 aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt
    11401 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg
    11461 caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa ggccatggag
    11521 tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga acagttcata
    11581 cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac
    11641 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
    11701 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
    11761 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
    11821 aaaggaaagg ctatcgttca agatgcccct gccgacagtg gtcccaaaga tggaccccca
    11881 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
    11941 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
    12001 ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa
    12061 ttaccacaac aaaacaaaca acaaacaaca ttacaattta ctattctagt cgaaatggca
    12121 tcctttgaaa atagtctgtc cgttttgatc gtcggggctg gccttggggg attagcagcc
    12181 gcaatagctc ttaggagaca gggccatgta gtaaagatct atgatagttc ctcattcaaa
    12241 gctgaactgg gtgctggcct tgctgtacct ccgaatacgc ttagaagcct tcaacagtta
    12301 ggttgtaata cagagaattt gaacggagtc gacaatctct gcttcacggc catgggttac
    12361 gacggctccg taggtatgat gaataacatg acagactaca gggaggctta tggcacatcc
    12421 tggattatgg ttcatcgagt ggatttacat aatgaactta tgcgtgtcgc acttgatccg
    12481 ggggggctgg gaccaccagc aacattacat ttaaatcaca gagtgacctt ctgcgacgtt
    12541 gacgcatgta cggtaacttt taccaacggg acgacacaaa gtgccgactt gatcgtaggg
    12601 gctgacggca tacgaagcac catacgtaga ttcgtcttgg aggaagatgt aactgtgcct
    12661 gccagcggga tcgtcggttt cagatggctg gtgcaagccg atgccttaga cccttaccct
    12721 gagttagatt ggattgttaa gaagcctcca ctcggtgctc gtctgatctc tacgccacag
    12781 aatccacagt ccggcgtggg gttggctgac cgtcgtacaa ttatcatcta cgcttgtagg
    12841 ggtggcacga tggttaatgt gttggctgtc cacgacgacg aaagagatca gaatacggca
    12901 gattggtcag tgccggcttc caaagatgat ctcttccgtg tattccatga ctaccaccca
    12961 agattcagac gtttgctcga acttgctcaa gatatcaatc tctggcaaat gagggtagtt
    13021 cccgtgctta aaaaatgggt gaacaagcgt gtctgtttat taggagatgc cgcccatgct
    13081 tctctcccca cgctgggtca agggttcgga atgggactgg aggacgccgt ggcactcggc
    13141 acattgttac ccaaaggaac tacggcttca cagatcgaaa cgagactggc cgtttacgag
    13201 caattacgaa aggatagggc tgagttcgta gcagcagaat cctacgagga acagtatgtc
    13261 cctgagatgc gtgggctcta tcttcgttcc aaagaactcc gagatcgagt gatgggatat
    13321 gatattaaag tggagtcaga aaaggttttg gagactctgc ttcgatcctc taattctgca
    13381 tgagcttgga atggatcttc gatcccgatc gttcaaacat ttggcaataa agtttcttaa
    13441 gattgaatcc tgttgccggt cttgcgacga ttatcatata atttctgttg aattacgtta
    13501 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
    13561 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
    13621 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattgcca agctaattct
    13681 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
    13741 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    13801 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    13861 caataatggg accgactcgc gctgtcagga gactagagcc aagctgatct cctttgcccc
    13921 ggagatcacc atggacgact ttctctatct ctacgatcta ggaagaaagt tcgacggaga
    13981 aggtgacgat accatgttca ccaccgataa tgagaagatt agcctcttca atttcagaaa
    14041 gaatgctgac ccacagatgg ttagagaggc ctacgcggca ggtctgatca agacgatcta
    14101 cccgagtaat aatctccagg agatcaaata ccttcccaag aaggttaaag atgcagtcaa
    14161 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
    14221 tactattcca gtatggacga ttcaaggctt gcttcataaa ccaaggcaag taatagagat
    14281 tggagtctct aagaaagtag ttcctactga atcaaaggcc atggagtcaa aaattcagat
    14341 cgaggatcta acagaactcg ccgtgaagac tggcgaacag ttcatacaga gtcttttacg
    14401 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc tcgtctactc
    14461 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
    14521 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
    14581 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
    14641 cgttcaagat gcccctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
    14701 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
    14761 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
    14821 aggaagttca tttcatttgg agaggactcc ggtattttta caacaattac cacaacaaaa
    14881 caaacaacaa acaacattac aatttactat tctagtcgaa atggtgagca agggcgagga
    14941 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
    15001 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
    15061 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
    15121 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
    15181 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
    15241 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
    15301 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
    15361 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
    15421 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
    15481 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
    15541 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
    15601 cgccgggatc actctcggca tggacgagct gtacaaggac gagctgtaag cttggaatgg
    15661 atcttcgatc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
    15721 gccggtcttg cgacgattat catataattt ctgttgaatt acgttaagca tgtaataatt
    15781 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
    15841 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
    15901 gcggtgtcat ctatgttact agatcgggaa ttgccaagct aattcttgaa gacgaaaggg
    15961 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
    16021 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
    16081 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatgggaccg
    16141 actcgcgctg tcatgagacc ggatcctgac aggatatatt ggcgggtaaa cctaagagaa
    16201 aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt
    16261 ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa
    16321 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc atctgaaaac
    16381 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt
    16441 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat
    16501 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac
    16561 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt
    16621 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac
    16681 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc
    16741 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca
    16801 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc
    16861 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc
    16921 aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac
    16981 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc
    17041 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag
    17101 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc
    17161 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac
    17221 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc
    17281 cgcccgcgca cctctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca
    17341 agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa
    17401 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat
    17461 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa
    17521 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg
    17581 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt
    17641 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt
    17701 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt
    17761 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta
    17821 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga
    17881 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg
    17941 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca
    18001 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga
    18061 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg
    18121 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg
    18181 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag
    18241 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc
    18301 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg
    18361 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca
    18421 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc
    18481 aggcgtaagc ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga
    18541 ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt
    18601 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca
    18661 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg
    18721 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca
    18781 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac
    18841 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag
    18901 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat
    18961 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa
    19021 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc
    19081 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta
    19141 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg
    19201 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg
    19261 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag
    19321 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt
    19381 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag
    19441 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc
    19501 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct
    19561 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc
    19621 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaagga
    19681 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac
    19741 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat
    19801 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt
    19861 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa
    19921 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg
    19981 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa
    20041 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt
    20101 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gtgacggtca cagcttgtct
    20161 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    20221 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
    20281 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
    20341 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
    20401 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    20461 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    20521 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    20581 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    20641 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    20701 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    20761 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    20821 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    20881 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    20941 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
    21001 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    21061 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    21121 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    21181 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtgatt atttgccgac
    21241 taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga
    21301 ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg
    21361 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt
    21421 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcacc
    21481 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc
    21541 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg
    21601 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat
    21661 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata
    21721 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc
    21781 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt
    21841 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc
    21901 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
    21961 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat
    22021 aatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
    22081 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
    22141 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
    22201 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt
    22261 acgaaccgaa caggcttatg tccactgggt tcgtgccttc atccgtttcc acggtgtgcg
    22321 tcacccggca accttgggta gcagcgaagt cgaggcattt ctgtcctggc tggaacagaa
    22381 cttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa
    22441 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc
    22501 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa
    22561 accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg
    22621 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt
    22681 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat
    22741 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg
    22801 gctggtggca ggatatattg tggtgtaaac ataacggatc cggtctca
//
