LOCUS       sequence-440610-        5842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter plasmid for Veillonella.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Goetting-Minesky MP, Kim J, White DT, Hayashi M, Rickard AH, Fenno 
            JC
  TITLE     Development of a small shuttle plasmid for use in oral Veillonella 
            and initial appraisal of potential for fluorescence-based 
            applications.
  JOURNAL   Lett Appl Microbiol. 2024 Aug 5;77(8):ovae069. doi: 
            10.1093/lambio/ovae069.
  PUBMED    39020263
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Lett Appl 
            Microbiol."; date: "2024-08-5"; volume: "77(8):ovae069. doi"; pages:
            " 10.1093/lambio/ovae069"
COMMENT     Sequence Label: pCF1148
FEATURES             Location/Qualifiers
     source          1..5842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      91..679
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     580..599
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    967..988
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1003..1008,1009..1026,1027..1033)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1041..1057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1046..1068
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1065..1081
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1065..1081
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1100..1120
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1102..1120
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4687..4706)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4688..4706)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4716..4733)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4716..4732)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4725..4747)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
       61 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
      121 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      181 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
      241 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
      301 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
      361 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
      421 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      481 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      541 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      601 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      661 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      721 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      781 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      841 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      901 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      961 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgctccc
     1021 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     1081 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tgggtaccat
     1141 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
     1201 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
     1261 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
     1321 agttattata aactacaagt ggatattgtg tcctgggatc cctgcagtta ttctaataat
     1381 ttttcatatt ctttttgttt tgtaatatca ttttgaattc ctacaaaata tgttttatct
     1441 tcaatttcca taggatcaat atttaattca ttccaaaaca ttgttccatc ttttttataa
     1501 ttttgaattt gtactgttac aggttcttta ttttgtaatg ctgttctaat attatctact
     1561 tcagcaggat ctgtatgttt tccttgtaaa aatcttgcat tttttcctaa aatttcttct
     1621 gtttcatatc ctgtcatttg tacaaatcct tgatttacat atacaatagg attatcttct
     1681 aatgcaggat ctgtaattac tactcctact cttacatgat ctaatgcttt tttaattact
     1741 tctaattgtc caggaattcc aaatgattga aatgatgctg atcccatcgt taaaacctct
     1801 tttcagaaaa tatgtacctg atatagctac ttcacattag ataaccatga attcaaaatg
     1861 atttcttgtg agttctatat cctctcaaat gacactgtca gtatagcaca gaaacaagtg
     1921 agattgaata gtcttacagt ctgaacgaca ttactaaata cgcatacgta ccggtgattt
     1981 ttgtcacata atctatacga cgctattaaa atgtctgatt atatctaaaa ctatatggtt
     2041 atgactttta gtaatgattg catgattttt ttgaagaatt ttggtatttt tggatccgat
     2101 tgttgcctag aagtgtctat caatccattt gcaaatatag taagcggcta tacttgccat
     2161 gatagacagt aagaaagata atagtaaatt caaaattagc acccccttcc tgttaccagt
     2221 ctagggtagg caacgattaa attatagcat atatgtaaat tagaaaatcg ctttggtatg
     2281 ttaaagtgat tttctaattt tgtcatgaaa aatgtaaaaa aaacttattg attatattgt
     2341 tgcttaagat agggaaaaaa ttcattgata atataagtcc cctatgagcc ctgtaggggc
     2401 tcctgaataa taagtaaaaa atgatggtaa aaatagacca tttttaatat actaaaaatg
     2461 tacaaaaaaa attcattgat aatataagtc ccctatgagc cccttagggg ctctcagata
     2521 attaagttaa gttagaggta tttatgagtg atattttttt agtaaaatgt acaaaaaaaa
     2581 ttcattgata atataagtcc cctatgagcc ccttaggggc tctcagataa ttaagttaag
     2641 ttagaggtat ttatgagtga tattgtgatg acagatacag agttatatga tgctaaagtt
     2701 aattggttta aggagcatga ttatgtagaa gtagaaccgt tagcttttta tagagattta
     2761 tatccaatag gatcatttca agttaaagga gattatagtc gtaaatgtgg taatgggatt
     2821 ttattgtata ggaatgaagg taaatttaag catagatata tatttgatga tttagaggag
     2881 attaagaatt ggattggaaa agaggatata tttgttagag gtggttcatt tataggtaaa
     2941 cgagttaaga atgaaaatgc gagtatgatt tatgcattga catttgatct tgatggtcaa
     3001 ggaatgaatg aattagaaat ggtcatgcaa tttatgcgta atggtactaa tattccaatg
     3061 gcaacatatg ttgtattaag tggtcatgga cttcatttat attatttatt ggataagcct
     3121 attcgagcga cgcaagatac gattcgtcag ttaaataagt taaaagaagg tatgactaaa
     3181 ttgatttgga atcgatatac atcaactatt gaaaaaccac aatatcagta ctgtttgcaa
     3241 ggttttagaa tggttggatc tgcgagtaaa atgggtgcta attatcctgt acgagcttat
     3301 aagactggtg atcgtatgac gattgaaaaa ttagtatctt ggatacctag attaaaagat
     3361 tgggacgaat atcgaattaa tttaactgaa cgttctactg tttctttagc aaaagcgaaa
     3421 gaaatctggc cagattggta tgaaagaaaa atagtgaagc aagaagataa taaatggcat
     3481 attaatcgtg ctttatatga ttggtggtta aataaaataa aagaaggcgc aacttatggg
     3541 cataggtatt tttgtattat gtgtttagca atttatgcga ttaaatgcaa catatcagag
     3601 gcagaattaa aacgagatgc ttattcttta ctagataaga tgaatgagtt atctaaaaaa
     3661 ggagatccat ctagtcaatt tacgaagatg gatatagagg cagcacttca agggtataaa
     3721 gaacatttta aaacctgggg aagggataaa attgcattaa tatcagctat tcctttaccc
     3781 attaataagc gaaattatcg tacacaagaa gagcatatga aagttattag tgctattagg
     3841 gatatagtta aacctaattg gcgtgaaggc aatggccgac caaaaggatg taaaaactcc
     3901 aattatccta aagctgagat tgtaaagcaa tggtgtttag atcatcctaa tggtaagaaa
     3961 attgattgtc atcgagatac tggattatca cgaatgacga ttaataaatg gtggaatata
     4021 tcgacggaag aattaaaacg acaaaaaata ataaataaga ttgagtttat cagaaataag
     4081 accatacaat taaatgaaac tcctgaagtt ctatatccta atacttcaga agagattgca
     4141 aaaatgagga agaatccaga tacagtttta gagctaaatc gattagggat aagctttgag
     4201 caactagaaa atcaacatga aatttatgta gaagatagtg ttacgtttca ggaactaata
     4261 gaaaaagaat ttggtgatga ttggattgat gctttaaatc tttatttgga ctacaaaatg
     4321 gagcgtaaat aagtgcagaa tatgtgcgac aatttataga tggtggttat ggattttatg
     4381 gagatggtat atggaacgat gggaaaatca agaatactgt ataaattatt aaagtaagcc
     4441 ttatattttt aaaaataaaa aaaggatagt ccttaattaa tgaaaggact atcctttttt
     4501 attttgatta gaggttatca gtagagctaa ttttttcttt ttcttcatta gctattactt
     4561 ggagtataat gcagtatttt aataatctaa gatatttatt ctctatgatt ataatgtaat
     4621 cattttcttt atttgataag gttaatggaa tattggcatc tctacccgcg gtggagctcc
     4681 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     4741 gactgggaaa accctggcgt tacccaactt aatcgccttg caggaattta accttggttg
     4801 tgttgctttt cggggcttaa atcaaggaaa aatccttgtt ttaagccttt caaaaagaaa
     4861 cacaaggtct ttgtactaac ctgtggttat gtataaaatt gtagatttta gggtaacaaa
     4921 aaacaccgta tttctacgat gtttttgctt aaatacttgt ttttagttac agacaaacct
     4981 gaagttaact atttatcaat tcctgcaatt cgtttacaaa acggcaaatg tgaaatccgt
     5041 cacatactgc gtgatgaact tgaattgcca aaggaagtat aattttgtta tcttctttat
     5101 aatatttccc catagtaaaa ataggaatca aataatcata tcctttctgc aaattcagat
     5161 taaagccatc gaaggttgac cacggtatca tagatacatt aaaaatgttt tccggagcat
     5221 ttggctttcc ttccattcta tgattgtttc cataccgttg cgtatcactt tcataatctg
     5281 ctaaaaatga tttaaagtca gacttacact cagtccaaag gctggaaaat gtttcagtat
     5341 cattgtgaaa tattgtatag cttggtatca tctcatcata tatccccaat tcaccatctt
     5401 gattgattgc cgtcctaaac tctgaatggc ggtttacaat cattgcaata taataaagca
     5461 ttgcaggata tagtttcatt cccttttcct ttatttgtgt gatatccact ttaacggtca
     5521 tgctgtaggt acaaggtaca cttgcaaagt agtggtcaaa atactctttt ctgttccaac
     5581 tatttttatc aattttttca aataccatct aagttccctc tcaaattcaa gtttatcgct
     5641 ctaatgaaca aagatattat accacatttt tgtgaatttt tcaacttgcc cacttcgact
     5701 gcactcccga cttaataact tcttgaacac ttgccgaaaa agaaaaactg ccgggtacgt
     5761 acccggggat cgatccccgg ccgagcgctc ttccgcttcc tcgctcactg actcgctgcg
     5821 ctcgagggat ctaggtgaag at
//
