LOCUS       sequence-440611-        5106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Veillonella shuttle plasmid with single selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Goetting-Minesky MP, Kim J, White DT, Hayashi M, Rickard AH, Fenno 
            JC
  TITLE     Development of a small shuttle plasmid for use in oral Veillonella 
            and initial appraisal of potential for fluorescence-based 
            applications.
  JOURNAL   Lett Appl Microbiol. 2024 Aug 5;77(8):ovae069. doi: 
            10.1093/lambio/ovae069.
  PUBMED    39020263
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Lett Appl 
            Microbiol."; date: "2024-08-5"; volume: "77(8):ovae069. doi"; pages:
            " 10.1093/lambio/ovae069"
COMMENT     Sequence Label: pCF1135
FEATURES             Location/Qualifiers
     source          1..5106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2985..3004)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2986..3004)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3014..3031)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3014..3030)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3023..3045)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4231..4819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4720..4739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc tcccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctgggta ccatgttact
      181 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
      241 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
      301 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
      361 tataaactac aagtggatat tgtgtcctgg gatccgattg ttgcctagaa gtgtctatca
      421 atccatttgc aaatatagta agcggctata cttgccatga tagacagtaa gaaagataat
      481 agtaaattca aaattagcac ccccttcctg ttaccagtct agggtaggca acgattaaat
      541 tatagcatat atgtaaatta gaaaatcgct ttggtatgtt aaagtgattt tctaattttg
      601 tcatgaaaaa tgtaaaaaaa acttattgat tatattgttg cttaagatag ggaaaaaatt
      661 cattgataat ataagtcccc tatgagccct gtaggggctc ctgaataata agtaaaaaat
      721 gatggtaaaa atagaccatt tttaatatac taaaaatgta caaaaaaaat tcattgataa
      781 tataagtccc ctatgagccc cttaggggct ctcagataat taagttaagt tagaggtatt
      841 tatgagtgat atttttttag taaaatgtac aaaaaaaatt cattgataat ataagtcccc
      901 tatgagcccc ttaggggctc tcagataatt aagttaagtt agaggtattt atgagtgata
      961 ttgtgatgac agatacagag ttatatgatg ctaaagttaa ttggtttaag gagcatgatt
     1021 atgtagaagt agaaccgtta gctttttata gagatttata tccaatagga tcatttcaag
     1081 ttaaaggaga ttatagtcgt aaatgtggta atgggatttt attgtatagg aatgaaggta
     1141 aatttaagca tagatatata tttgatgatt tagaggagat taagaattgg attggaaaag
     1201 aggatatatt tgttagaggt ggttcattta taggtaaacg agttaagaat gaaaatgcga
     1261 gtatgattta tgcattgaca tttgatcttg atggtcaagg aatgaatgaa ttagaaatgg
     1321 tcatgcaatt tatgcgtaat ggtactaata ttccaatggc aacatatgtt gtattaagtg
     1381 gtcatggact tcatttatat tatttattgg ataagcctat tcgagcgacg caagatacga
     1441 ttcgtcagtt aaataagtta aaagaaggta tgactaaatt gatttggaat cgatatacat
     1501 caactattga aaaaccacaa tatcagtact gtttgcaagg ttttagaatg gttggatctg
     1561 cgagtaaaat gggtgctaat tatcctgtac gagcttataa gactggtgat cgtatgacga
     1621 ttgaaaaatt agtatcttgg atacctagat taaaagattg ggacgaatat cgaattaatt
     1681 taactgaacg ttctactgtt tctttagcaa aagcgaaaga aatctggcca gattggtatg
     1741 aaagaaaaat agtgaagcaa gaagataata aatggcatat taatcgtgct ttatatgatt
     1801 ggtggttaaa taaaataaaa gaaggcgcaa cttatgggca taggtatttt tgtattatgt
     1861 gtttagcaat ttatgcgatt aaatgcaaca tatcagaggc agaattaaaa cgagatgctt
     1921 attctttact agataagatg aatgagttat ctaaaaaagg agatccatct agtcaattta
     1981 cgaagatgga tatagaggca gcacttcaag ggtataaaga acattttaaa acctggggaa
     2041 gggataaaat tgcattaata tcagctattc ctttacccat taataagcga aattatcgta
     2101 cacaagaaga gcatatgaaa gttattagtg ctattaggga tatagttaaa cctaattggc
     2161 gtgaaggcaa tggccgacca aaaggatgta aaaactccaa ttatcctaaa gctgagattg
     2221 taaagcaatg gtgtttagat catcctaatg gtaagaaaat tgattgtcat cgagatactg
     2281 gattatcacg aatgacgatt aataaatggt ggaatatatc gacggaagaa ttaaaacgac
     2341 aaaaaataat aaataagatt gagtttatca gaaataagac catacaatta aatgaaactc
     2401 ctgaagttct atatcctaat acttcagaag agattgcaaa aatgaggaag aatccagata
     2461 cagttttaga gctaaatcga ttagggataa gctttgagca actagaaaat caacatgaaa
     2521 tttatgtaga agatagtgtt acgtttcagg aactaataga aaaagaattt ggtgatgatt
     2581 ggattgatgc tttaaatctt tatttggact acaaaatgga gcgtaaataa gtgcagaata
     2641 tgtgcgacaa tttatagatg gtggttatgg attttatgga gatggtatat ggaacgatgg
     2701 gaaaatcaag aatactgtat aaattattaa agtaagcctt atatttttaa aaataaaaaa
     2761 aggatagtcc ttaattaatg aaaggactat ccttttttat tttgattaga ggttatcagt
     2821 agagctaatt ttttcttttt cttcattagc tattacttgg agtataatgc agtattttaa
     2881 taatctaaga tatttattct ctatgattat aatgtaatca ttttctttat ttgataaggt
     2941 taatggaata ttggcatctc tacccgcggt ggagctccaa ttcgccctat agtgagtcgt
     3001 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3061 cccaacttaa tcgccttgca ggaatttaac cttggttgtg ttgcttttcg gggcttaaat
     3121 caaggaaaaa tccttgtttt aagcctttca aaaagaaaca caaggtcttt gtactaacct
     3181 gtggttatgt ataaaattgt agattttagg gtaacaaaaa acaccgtatt tctacgatgt
     3241 ttttgcttaa atacttgttt ttagttacag acaaacctga agttaactat ttatcaattc
     3301 ctgcaattcg tttacaaaac ggcaaatgtg aaatccgtca catactgcgt gatgaacttg
     3361 aattgccaaa ggaagtataa ttttgttatc ttctttataa tatttcccca tagtaaaaat
     3421 aggaatcaaa taatcatatc ctttctgcaa attcagatta aagccatcga aggttgacca
     3481 cggtatcata gatacattaa aaatgttttc cggagcattt ggctttcctt ccattctatg
     3541 attgtttcca taccgttgcg tatcactttc ataatctgct aaaaatgatt taaagtcaga
     3601 cttacactca gtccaaaggc tggaaaatgt ttcagtatca ttgtgaaata ttgtatagct
     3661 tggtatcatc tcatcatata tccccaattc accatcttga ttgattgccg tcctaaactc
     3721 tgaatggcgg tttacaatca ttgcaatata ataaagcatt gcaggatata gtttcattcc
     3781 cttttccttt atttgtgtga tatccacttt aacggtcatg ctgtaggtac aaggtacact
     3841 tgcaaagtag tggtcaaaat actcttttct gttccaacta tttttatcaa ttttttcaaa
     3901 taccatctaa gttccctctc aaattcaagt ttatcgctct aatgaacaaa gatattatac
     3961 cacatttttg tgaatttttc aacttgccca cttcgactgc actcccgact taataacttc
     4021 ttgaacactt gccgaaaaag aaaaactgcc gggtacgtac ccggggatcg atccccggcc
     4081 gagcgctctt ccgcttcctc gctcactgac tcgctgcgct cgagggatct aggtgaagat
     4141 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4201 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4261 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4321 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4381 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4441 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4501 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4561 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4621 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4681 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4741 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4801 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4861 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4921 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4981 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     5041 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     5101 cgcaat
//
