LOCUS       sequence-440613-       17153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for hA3A-nCas9 expression in Aspergillus oryzae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17153)
  AUTHORS   Zhao F, Sun C, Liu Z, Cabrera A, Escobar M, Huang S, Yuan Q, Nie Q, 
            Luo KL, Lin A, Vanegas JA, Zhu T, Hilton IB, Gao X
  TITLE     Multiplex Base-Editing Enables Combinatorial Epigenetic Regulation 
            for Genome Mining of Fungal Natural Products.
  JOURNAL   J Am Chem Soc. 2023 Jan 11;145(1):413-421. doi: 
            10.1021/jacs.2c10211. Epub 2022 Dec 21.
  PUBMED    36542862
REFERENCE   2  (bases 1 to 17153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.2c10211"; journalName: "J Am Chem Soc"; date: 
            "2023-01-11- 11"; volume: "145"; issue: "1"; pages: "413-421"
COMMENT     Sequence Label: paoB-CBE
FEATURES             Location/Qualifiers
     source          1..17153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(2324..2912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2404..2423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3083..3874,3875..3943))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3706..3725
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3944..4048)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6955..6976
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             8417..8437
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8447..8695
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8726..9319
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="EASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTSV
                     KMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSWG
                     CAGEVRAFLQENTHVRLRIFAARIFDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWDT
                     FVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             11132..13516
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             13520..13540
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     14163..14180
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14297..14318
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14333..14338,14339..14356,14357..14363)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14371..14387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14376..14398
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14395..14411
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14395..14411
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      14428..15770
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(15391..15438)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     15811..15829
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(15867..15889)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     15989..16008
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        16032..16247
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             16248..17051
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 attccgcggt tttttggggt agtcggaaga taattcgaat ggaggcgcag gactcgagtc
       61 ggaagattgt ctttcgtcga tcggtgacga aggacgcgaa gagggcctgg accccttgtt
      121 cagtaaaggc ctgcttgcct cttcagccat gatggctggg ggggccggat aagtggggga
      181 gatggggaga gggatgtgga ggagataaca cgatagcaat acgttatggg tagataagag
      241 agataaaaga tgatggcgtt cgtagattga gaaaagaaag ctcccggtgg tgatgagttc
      301 ggtagaaggg gacgtaaggc taagaattaa aatatttagc ttgcgtatgg acaacgcacg
      361 caggatgaat tggccagacc tggaagtata gcctatcagt ttgaatcacc gtgatgatcg
      421 gcaatcatac cacgctaacc cgaactggac aaggcgtggt gtggagacgg aaaaagcgga
      481 tcacattgga ggagtcacac ttaacgcttt caagctaact gactgactcg acggtcgagc
      541 taaggtacgt actatttctt ttttcttttc tctttgtctc ttgttgctgc tgttgactct
      601 ctctcttcta gtccgataag aactgagtca tttcaatcct ctggttctta ctctatatat
      661 tccccctccc ctactaccca attgcttctc cgcatactac cggaaattat ctcatcatga
      721 cttgcggtct gaagctggcc gccgctcgtt acggcaacca taccttgcgc cagaggattc
      781 cactcaacgc tgtgcgccga tacacttctc acactgctac ttctacgact ccccccacct
      841 cgccattcgc tcctcgccat tttctttcca tcgctgatct tacctcaacg gagttcgcaa
      901 ccctcgtccg taatgcatct tcacacaaac gaaccatcaa gtcgggatca atccctcaga
      961 acctgcttgg ttctatgacc ggacagactg tagctatgct gttcagcaaa cgcagtacca
     1021 gaaccaggat atctactgaa ggggctgtgg tgcgcttggg aggacatcca atgtttctgg
     1081 gcaaggatga tattcaactc ggtgtcaatg agtccttgta tgactctgcg gttgtaatct
     1141 cctccatggt ctcttgtatt gtagcccgag tcggcaagca cgctgaggtt gcagaccttg
     1201 ccaagcattc aaccgtgcct gttatcaacg ctctctgtga ctccttccac cctctccagg
     1261 ccattgccga ttttcagacc atctacgaaa catttacacc taaggcccat cgctctgaca
     1321 gtttgggtct ggagggcctc aagattgcct gggtcggtga tgcaaacaac gttctgttcg
     1381 acatggcaat tgctgctacc aagatgggta ttgatatcgc cgttgcgact ccgaagggct
     1441 atgagattcc tgccccgatg ttggagctca tcaagcaggc cagcaacggt gtctcaaagc
     1501 caggaaagat tattgagacc aacgttcctg aggaggcggt taagggtgcg gatatcctgg
     1561 taactgacac ctgggtttcc atgggacagg aggcagagtc gatcaagcgg gtgaaggact
     1621 tcgagggctt ccagatcact tcggaacttg ccaagcgcgg tggtgcgaac gaaggctgga
     1681 agttcatgca ctgcctgcct cgtcaccctg aggaagtcag cgatgaagtt ttctacagcc
     1741 cacggtcctt ggtcttcccc gaggcggaga atcggttgtg ggctgcgatc tcggccatgg
     1801 agggctttgt tgtcaataag ggaaggattg agtaagcctt aaaaaagaaa taaatcataa
     1861 aaatctatat agtccaaagc tcaattctga atggattaat ggaccgttgt ttgactttcc
     1921 ataccgaagt atgtagtata gaataccaca aacatgtaaa cacgtctata tatgtagaag
     1981 taaaaccgca cttactacgg aggtataaaa aaataaagcc gttggtgccc gcattcacat
     2041 gtcacgggcg gagttgccga caaaacaagg ccaacaaccc tccaaaatct catgagctgc
     2101 tgcatccatc ataccattaa tgagcttgcc actctcttag tgccctcttc ctatatttat
     2161 tgccctccgt ggccccaggc aacctagatg gaggtatgta tttccctgct gacgggtaat
     2221 tcttctgagg tttcgcaatg gctgtaggtc tctctacata tgacatgtga gcaaaaggcc
     2281 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2341 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2401 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2461 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2521 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2581 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2641 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2701 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2761 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2821 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2881 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2941 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3001 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3061 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3121 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3181 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaacca cgctcaccgg
     3241 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3301 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3361 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3421 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3481 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3541 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3601 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3661 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3721 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3781 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3841 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3901 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3961 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4021 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     4081 ctagctgtac aaaaaagcag gctttaaagg aaccaattca gtcgactgga tccggtacca
     4141 aggtcgggca ggaactgcag attttgacct agttaacaag gcacatcatg ctatcacttc
     4201 caacactctt gctgagcgcg ttctgccttg cctttctggt tctacgaagt atccagtccc
     4261 gcctgcaacg ccgcagaaac gccaaaaggt ggggttgtca accgccagct agagctccat
     4321 cgggattctt tggcattccg ccgttcctcg gtggtcgcag ataactccct aaattccctg
     4381 agctaactct aagtcgacca tgccgtttat ggttagcgcc tcccaaaaag gaatggccga
     4441 cttagagtta cctcttgacc gactttttct ttcctccccc ttacatttcg ttaccacaac
     4501 acattcctat atccaaactc caggtacata actagtcgaa atctctttta aatctagtca
     4561 agaactagat acttaacctt catactgctt agagatagcc tttgaagctc ttatttcgct
     4621 ctttgtctca ctctcacctt cctcctcctc ctccaatcct ttttgctcgg gactagtcca
     4681 agactagtca acgattagta tgccgagcat tcgcgataac gatctccgaa agtccccaga
     4741 gtactgtcac tatctcgagg cagttaagga cggggagctt acgctgccgg atttcaagat
     4801 agtaagccga cccgactagc tttaagccta gttactgact agtccacagg acgacaatgg
     4861 cgtgcctgat atccatccat atgaagtcta ctgccgagtg aagggatgcc tcaagcgtac
     4921 agtgagtcta ctgctatact agtttctgac tagttattaa ctagttgaca ggttccctct
     4981 gccaacagaa acatattggt caagcacttg aaggacaaga actcccacgg catggagttt
     5041 acattgcaca atggacctcc cactatgaag gaactgatgg aggccaaagg caagtcctat
     5101 ctagattact tggtgactag tccctgacta gtcaagtagc atggtatgaa ggcttgtttg
     5161 aaggcactgt tctcccaacc ccgactccta ccaagaagcg caagcgagct gcgtaagttt
     5221 ctgtgagtct aactagtgta ttagctaata tatagcagca ccaagtccaa ggaccacaat
     5281 actaagggtg tcgagaattc gtgagtttct tctcccattt caactagtcc ttgactagtc
     5341 actaactact ttgcagcaac gagggtgaag ctggaaatga tcaggacaat ggcgagggcc
     5401 cgtaagtaca gccattcaat gcagactagt tgctaactaa tctgtgacta gttcaagtgg
     5461 tccgtacgcc gtgcataccc ctgtgactgg taggaatttg agcaagcctg tcttgccgcg
     5521 cgatgagaaa ggaaaggcaa gttacattca gccccgtacc taggatcagt gctaatttat
     5581 aacctctagc cactctttat gcagatccgc cgtgagggta gcaaggcagc taaatcagct
     5641 ggtgagaaag gaaccatacc ctgcaagacc tgtcgcaacg caaagggcaa aggtaagcta
     5701 tccaagctag tttgggacta gattctaact agtctcagca ccgtgtggtt caaagccata
     5761 ttgcgagttt tggcgctttt tctcatcgat tgacgaggca aagggagcga gtatgcaacc
     5821 tcgtaagtca gactcagaca aagaccaact agttattgac cagtcttcta gaaggctctg
     5881 ttgtggatct tgaggccctg gagagttcct ccaacaatcc ggagacaagc aagtcctcgt
     5941 cggactagtc actaactaga ctctaactag ttgcagacat ggataatgca aaagagacaa
     6001 gcaatgaaga aagtggtaag acatttctcc tttgtggttc tggactagtc tttgactagt
     6061 cacagtctta aacaaggaaa atgagcatga aaatgaggag gaaaaggctg ctgagcccga
     6121 ggaagtgcag ggtgatggca gacatggtag gttaatacct tgttagttat tgctagtcac
     6181 tgactagtca ataactagtc tctgaacacc ttgcaatcac tccgtttgcg cagctgaaca
     6241 gtggtgagga taatagtagt aagttattct agcttcagag ttataggaga ctagatacta
     6301 actagtatta gttgcaacta acctggatct cagagacttt ggcctcaatc tagaatctat
     6361 ctagttgtca actagactgt ggtatcattg tcttttattt tcctagtcct ggaactagct
     6421 tctaactagt ctccctaata tgtggctgtc ttgttttttt tttgtttccc tacccggata
     6481 tctagtcccc ttctaggttc tgttaacctc tcgggctctg atttagttta acgcaaacct
     6541 gagattagtt tctaactagt ctctaggttt tctatccacc tttaattgta ataataaata
     6601 caagcaacgt ttatacgtca aaagcattta taaactttta ccctaaagta gcttgcttgt
     6661 gtgtttagtt tataattagt ctcttattaa tttgatgtag gtaagcccgc cacaaatata
     6721 tatttttaac aagataccgt ggaaaaactt cgtgctatca caaaacagta tacaaaaaat
     6781 aagctggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6841 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     6901 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6961 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
     7021 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7081 cgaattttaa caaaatgggg tgacgatgag ccgctcttgc atctttgttt gtattatact
     7141 gtctttcttg ttacacataa ttattctaga atgccccacc gttacatacg ggacacagcc
     7201 atttacatat gcatgtggat tacgagctaa cgagttcatt caaatctcag aactatcaca
     7261 taatcatcat tcccctatcg tcaaagaccg taagacaaat ccggttcatg cactgaaccc
     7321 attcgggtag tgagtcattt actcagcaca ctcgcgctga cgctcgtcga acaccttcaa
     7381 tgcctcctcg gcagccttga caccactgag aaccatggca ccgaaggtag ggcccatgcg
     7441 gttaaagcca tcaatttcag acagctccat accgccgatt atcaagccct tagtaacctc
     7501 gcgggtgttc ttgacgatgg catcctcgag acagcagaat caccgcccaa gttaagcctt
     7561 tgtgctgatc atgctctcga acgggccaag ttcgggaaaa gcaaaggagc gtttagtgag
     7621 gggcaatttg actcacctcc caggcaacag atgagggggg caaaaagaaa gaaattttcg
     7681 tgagtcaata tggattccga gcatcatttt cttgcggtct atcttgctac gtatgttgat
     7741 cttgacgctg tggatcaagc aacgccactc gctcgctcca tcgcaggctg gtcgcagaca
     7801 aattaaaagg cggcaaactc gtacagccgc ggggttgtcc gctgcaaagt acagagtgat
     7861 aaaagccgcc atgcgaccat caacgcgttg atgcccagct ttttcgatcc gagaatccac
     7921 cgtagaggcg atagcaagta aagaaaagct aaacaaaaaa aaatttctgc ccctaagcca
     7981 tgaaaacgag atggggtgga gcagaaccaa ggaaagagtc gcgctgggct gccgttccgg
     8041 aaggtgttgt aaaggctcga cgcccaaggt gggagtctag gagaagaatt tgcatcggga
     8101 gtggggcggg ttacccctcc atatccaatg acagatatct accagccaag ggtttgagcc
     8161 cgcccgctta gtcgtcgtcc tcgcttgccc ctccataaaa ggatttcccc tccccctccc
     8221 acaaaatttt ctttcccttc ctctccttgt ccgcttcagt acgtatatct tcccttccct
     8281 cgcttctctc ctccatcctt ctttcatcca tctcctgcta acttctctgc tcagcacctc
     8341 tacgcattac tagccgtagt atctgagcac ttctcccttt tatattccac aaaacataac
     8401 acgcggccgc atgggaccta agaaaaagag gaaggtggcg gccgctacta atctgagcga
     8461 catcattgag aaggagactg ggaaacagct ggtcattcag gagtccatcc tgatgctgcc
     8521 tgaggaggtg gaggaagtga tcggcaacaa gccagagtct gacatcctgg tgcacaccgc
     8581 ctacgacgag tccacagatg agaatgtgat gctgctgacc tctgacgccc ccgagtataa
     8641 gccttgggcc ctggtcatcc aggattctaa cggcgagaat aagatcaaga tgctgagcgg
     8701 aggatccgga ggatctggag gcagcgaagc cagcccagca tccgggccca gacacttgat
     8761 ggatccacac atattcactt ccaactttaa caatggcatt ggaaggcata agacctacct
     8821 gtgctacgaa gtggagcgcc tggacaatgg cacctcggtc aagatggacc agcacagggg
     8881 ctttctacac aaccaggcta agaatcttct ctgtggcttt tacggccgcc atgcggagct
     8941 gcgcttcttg gacctggttc cttctttgca gttggacccg gcccagatct acagggtcac
     9001 ttggttcatc tcctggagcc cctgcttctc ctggggctgt gccggggaag tgcgtgcgtt
     9061 ccttcaggag aacacacacg tgagactgcg tatcttcgct gcccgcatct tcgattacga
     9121 ccccctatat aaggaggcac tgcaaatgct gcgggatgct ggggcccaag tctccatcat
     9181 gacctacgat gaatttaagc actgctggga cacctttgtg gaccaccagg gatgtccctt
     9241 ccagccctgg gatggactag atgagcacag ccaagccctg agtgggaggc tgcgggccat
     9301 tctccagaat cagggaaact ctggaggatc tagcggagga tcctctggca gcgagacacc
     9361 aggaacaagc gagtcagcaa caccagagag cagtggcggc agcagcggcg gcagcgacaa
     9421 gaagtatagc atcgggctgg ccattggaac gaactcggtt ggttgggctg tgattacgga
     9481 cgaatacaag gtgccatcca agaagtttaa ggtcctggga aacaccgacc gtcactcaat
     9541 caagaagaat ctcattggag ccctgctctt cgatagtggg gagaccgccg aagctactcg
     9601 actgaagcga acggctcgcc ggcgttatac acgacgcaag aatcgcatct gctacctcca
     9661 ggagattttc agcaacgaaa tggataaggt tgatgactca ttctttcatc gactcgaaga
     9721 aagtttcttg gtcgaggagg ataagaagca cgagcgccat ccgatctttg gtaacattgt
     9781 ggatgaggtt gcctatcacg aaaagtaccc aactatctat catcttcgta agaagctggt
     9841 cgatagcacg gacaaggctg atttgcgact tatctacctg gcactcgcgc acatgattaa
     9901 gttccgcggc cattttctta tcgagggtga cctgaacccc gataattctg acgttgataa
     9961 gctcttcatc cagttggtcc aaacctacaa tcagctgttt gaggaaaacc ctattaatgc
    10021 atctggcgtg gacgccaagg ctatcctttc ggcgcgcctg tctaagtcgc ggcgtttgga
    10081 gaaccttatc gcacaactcc ccggcgaaaa gaagaacggc ctcttcggta atttgattgc
    10141 gttgtcactt ggtctgactc ctaacttcaa gagtaatttt gacctggcag aggatgcgaa
    10201 gctccagttg tctaaggata cgtatgatga cgatctcgac aacttgcttg cccaaatcgg
    10261 tgaccagtac gctgatcttt tcctggccgc taagaatctc tcagatgcaa tcctgctcag
    10321 tgacattttg cgggtcaaca ccgagattac taaggccccc ctgtcagcta gtatgatcaa
    10381 gcggtatgat gagcaccatc aggacctcac cttgcttaag gccctcgtgc gtcagcaatt
    10441 gcctgagaag tataaggaaa tcttctttga ccaatccaag aacggatacg cagggtatat
    10501 tgatggcggt gcgagccagg aggaattcta caagtttatc aagccgattt tggagaagat
    10561 ggacggcact gaggaactgc tcgtcaagct gaatcgcgaa gatttgcttc gtaagcaacg
    10621 aacgttcgac aacggctcca tcccgcacca gattcatctg ggcgagctcc acgccatcct
    10681 tcgacgccag gaagatttct acccatttct gaaggacaac cgtgagaaga tcgaaaagat
    10741 tcttacattc cgaatcccct actatgtggg acctttggcc cgtgggaatt cccgatttgc
    10801 ttggatgacc cgaaagagcg aggaaaccat cactccgtgg aacttcgagg aagtcgtgga
    10861 caagggtgca tccgcgcaga gcttcattga gcggatgacc aattttgata agaaccttcc
    10921 gaatgaaaag gtcctgccaa agcattcgct gctctacgag tatttcaccg tgtataacga
    10981 actgactaag gtcaagtacg tgacggaggg aatgcggaag ccagccttcc tctcagggga
    11041 acaaaagaag gctatcgtcg atttgctttt taagaccaat cgtaaagtga ctgttaagca
    11101 gctgaaggag gattatttca agaagattga atgtttcgac tccgtcgaga tcagcggcgt
    11161 ggaagatcgc tttaacgctt ccctcggtac ctaccacgac ctgctcaaga tcattaagga
    11221 caaggatttc ctcgataacg aggaaaatga ggacatcttg gaagatattg tcctcacgtt
    11281 gacacttttt gaggaccgcg aaatgatcga ggaacggctc aagacatatg cccatttgtt
    11341 cgacgataag gtgatgaagc agctgaagcg gcgtcgatac accggatggg gtcgccttag
    11401 ccggaagctg atcaacggca ttcgagataa gcaatctggt aagactatct tggatttcct
    11461 taagtcggac ggcttcgcca accgcaattt tatgcagctt attcacgacg attccctgac
    11521 gttcaaggag gacatccaga aggcacaagt ctcaggacaa ggggattccc tgcacgagca
    11581 tatcgccaac ctggctggat ccccggcgat caagaagggg attcttcaga ccgtcaaggt
    11641 tgtcgacgag ctggtcaagg tgatgggccg tcataagcca gaaaacatcg tgattgagat
    11701 ggcccgagaa aatcagacca ctcaaaaggg tcagaagaac agccgcgagc ggatgaagcg
    11761 gatcgaggaa ggcattaagg aacttggttc tcagatcctg aaggagcacc ctgttgaaaa
    11821 cacacagctc caaaatgaga agctgtatct ctactatttg caaaatggac gcgacatgta
    11881 cgtcgatcag gagctcgaca ttaaccggtt gtcggactac gatgttgacc atatcgtccc
    11941 gcaatccttc cttaaggacg atagcattga taacaaggtg ctgactcgct cagataagaa
    12001 ccggggcaag tccgacaatg ttccaagcga ggaagtggtt aagaagatga agaactactg
    12061 gcgccaattg cttaatgcca agctcatcac acagcgcaag tttgacaact tgaccaaggc
    12121 cgagcgggga gggctgagtg aactcgataa ggctggcttc atcaagcgtc aactcgtgga
    12181 gacgcgacag atcacaaagc acgttgctca gattctggac tcccggatga acacaaagta
    12241 cgacgagaat gataagctca tccgtgaagt taaggtcatt accctcaagt ctaagttggt
    12301 gtcggatttc cgcaaggact tccaatttta taaggttcgg gagatcaaca attatcacca
    12361 tgcacatgat gcgtacctca acgcagtcgt gggaactgcg ctcatcaaga agtatcccaa
    12421 gttggagtcc gaattcgtct acggggatta taaggtttac gacgtccgca agatgatcgc
    12481 caagagtgag caggaaattg gcaaggccac ggctaagtat ttcttttact ccaacatcat
    12541 gaatttcttt aagacggaga tcacactcgc caatggagaa atccgtaagc gacctttgat
    12601 tgagaccaac ggcgagactg gtgaaatcgt ttgggataag gggcgcgact tcgctaccgt
    12661 gcggaaggtt ctgagcatgc cgcaagtcaa tatcgtcaag aaaaccgagg tgcagacagg
    12721 cggtttctct aaggaatcga ttcttccaaa gcgtaactct gacaagctga tcgctcgaaa
    12781 gaaggattgg gaccccaaga agtatggagg gttcgattct cctacagtgg catactcggt
    12841 tctcgttgtc gcgaaggttg agaagggaaa gtctaagaag ctgaagtcgg tcaaggaact
    12901 gctcgggatc accattatgg agcgctccag cttcgaaaag aatcccatcg actttctcga
    12961 ggccaagggc tataaggaag tcaagaagga tcttatcatt aagctgccta agtactcttt
    13021 gttcgagctt gaaaacggtc gaaagcgaat gctcgcatcg gcaggagagt tgcagaaggg
    13081 gaatgaattg gcacttccct caaagtacgt gaacttcctg tatctcgcgt cccactacga
    13141 gaagctgaag ggtagccctg aggacaacga acagaagcaa ctttttgttg agcaacacaa
    13201 gcattatctg gatgagatca ttgaacagat ttcagagttc agtaagcgcg tcatcctcgc
    13261 cgatgctaat ctcgacaagg tgttgtcggc ctacaacaag caccgtgaca agccgatccg
    13321 agagcaggct gaaaatatca ttcatctgtt caccctcact aacttgggag caccagcagc
    13381 gttcaagtat tttgatacga caatcgaccg taagcgatac acgtccacaa aggaggtgct
    13441 tgatgcgacc ctgattcatc aatccatcac tgggctctat gaaacccgta tcgaccttag
    13501 tcaactgggg ggcgaccctc ccaagaagaa gcgcaaggtc tgagcggaca ttcgatttat
    13561 gccgttatga cttccttaaa aaagccttta cgaatgaaag aaatggaatt agacttgtta
    13621 tgtagttgat tctacaatgg attatgattc ctgaacttca aatccgctgt tcattattaa
    13681 tctcagctct tcccgtaaag ccaatgttga aactattcgt aaatgtacct cgttttgcgt
    13741 gtaccttgct tatcacgtga tattacatga cctggacaga gttctgcgcg aaagtcataa
    13801 cgtaaatccc gggcggtagg tgcgtcccgg gcggaaggta gttttctcgt ccaccccaac
    13861 gcgtttatca acctcaactt tcaacaacca tcatgccacc aaaagcgcgt aaaacaaagc
    13921 gagatttgat tgagcaagag ggcaggatcc aatgcgcgat tcaagacatt aaaaatggaa
    13981 aatttcaaaa aattgcgccc gcagcgcgtg catacaaaat tcatttattt aaatggttcc
    14041 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    14101 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    14161 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    14221 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    14281 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    14341 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    14401 acagctatga ccatgattac gccactgaac gaagcatctg tgcttcattt tgtagaacaa
    14461 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
    14521 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa
    14581 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
    14641 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt
    14701 tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt
    14761 tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc
    14821 gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac
    14881 tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag
    14941 gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga
    15001 tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct
    15061 atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata
    15121 gttcttacta caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag
    15181 aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat
    15241 atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc
    15301 gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt
    15361 cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag agaataggaa
    15421 cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga
    15481 gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata
    15541 tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt
    15601 ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg
    15661 gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca
    15721 attggattag tctcatcctt caatgctatc atttcctttg atattggatc atactaagaa
    15781 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    15841 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    15901 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    15961 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
    16021 cataccacct tttcaattca tcattttttt tttattcttt tttttgattt cggtttcctt
    16081 gaaatttttt tgattcggta atctccgaac agaaggaaga acgaaggaag gagcacagac
    16141 ttagattggt atatatacgc atatgtagtg ttgaagaaac atgaaattgc ccagtattct
    16201 taacccaact gcacagaaca aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta
    16261 catataagga acgtgctgct actcatccta gtcctgttgc tgccaagcta tttaatatca
    16321 tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc aaggaattac
    16381 tggagttagt tgaagcatta ggtcccaaaa tttgtttact aaaaacacat gtggatatct
    16441 tgactgattt ttccatggag ggcacagtta agccgctaaa ggcattatcc gccaagtaca
    16501 attttttact cttcgaagac agaaaatttg ctgacattgg taatacagtc aaattgcagt
    16561 actctgcggg tgtatacaga atagcagaat gggcagacat tacgaatgca cacggtgtgg
    16621 tgggcccagg tattgttagc ggtttgaagc aggcggcaga agaagtaaca aaggaaccta
    16681 gaggcctttt gatgttagca gaattgtcat gcaagggctc cctatctact ggagaatata
    16741 ctaagggtac tgttgacatt gcgaagagcg acaaagattt tgttatcggc tttattgctc
    16801 aaagagacat gggtggaaga gatgaaggtt acgattggtt gattatgaca cccggtgtgg
    16861 gtttagatga caagggagac gcattgggtc aacagtatag aaccgtggat gatgtggtct
    16921 ctacaggatc tgacattatt attgttggaa gaggactatt tgcaaaggga agggatgcta
    16981 aggtagaggg tgaacgttac agaaaagcag gctgggaagc atatttgaga agatgcggcc
    17041 agcaaaacta aaaaactgta ttataagtaa atgcatgtat actaaactca caaattagag
    17101 cttcaattta attatatcag ttattaccct atgcggtgtg aaataattta aat
//
