LOCUS       sequence-440648-        1464 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of TNRC6A-silencing domain (SD) with 
            S1332A/S1346A/S1616A/S1691A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Munakata F, Suzawa M, Ui-Tei K
  TITLE     Identification of Phosphorylated Amino Acids in Human TNRC6A 
            C-Terminal Region and Their Effects on the Interaction with the 
            CCR4-NOT Complex.
  JOURNAL   Genes (Basel). 2021 Feb 13;12(2):271. doi: 10.3390/genes12020271.
  PUBMED    33668648
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2021-02-13"; volume: "12(2):271. doi"; pages: " 
            10.3390/genes12020271"
COMMENT     Sequence Label: 
            pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A
FEATURES             Location/Qualifiers
     source          1..1464
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    549..560
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 atgccactcc atcagccagc catgaagtct ttccttgaca atgtcatgcc ccacactaca
       61 cctgagctgc aaaaagggcc atcaccaata aatgctttca gcaacttccc tataggcttg
      121 aactcaaact tgaatgtaaa tatggatatg aacagtatta aagagccaca gtcaagacta
      181 aggaagtgga cgacagtgga cagcatttct gtgaacacat ctttggatca aaactccagc
      241 aaacatggtg ctatttcaag tggtttcagg ctggaagagt ctccatttgt tccctatgac
      301 tttatgaaca gcagtacttc accagccgct cctccaggtt caataggaga tggctggcca
      361 cgtgccaaag ctcctaacgg ctctagcagt gttaattggc caccagaatt tcgtcctggt
      421 gagccatgga aaggttatcc aaacattgac cctgaaactg acccttacgt cactcctggc
      481 agtgtcataa acaatctttc aattaatact gtgcgggaag ttgaccacct cagggacagg
      541 aacagtgggt catcctcatc cttgaacacc acgctgcctt caactagtgc ctggtcatcc
      601 attcgtgcct ccaactacaa cgttcccctc agcagtacag cacaaagcac ttcagccaga
      661 aatagtgatt ccaaattgac atggtctcct ggttcagtta caaacacctc tctggctcat
      721 gagctgtgga aggtcccttt gccacctaaa aacatcactg ctccgtcccg cccacctccg
      781 ggactgactg gtcagaagcc acccttgtct acgtgggata attctcccct tcgtataggt
      841 ggaggatggg gaaattctga cgccagatat accccaggtt ccagctgggg tgagagcagc
      901 tcagggagaa taacaaattg gcttgttcta aaaaacctta cacctcagat cgatggctca
      961 actctgcgca ctctgtgcat gcagcacggc ccgctgatca cattccacct gaacctccct
     1021 cacggaaatg ctctggtccg ctacagttca aaagaagagg tagtgaaggc acaaaagtct
     1081 ctgcacatgt gtgtactggg gaacactact attcttgctg agtttgccag tgaagaggag
     1141 atcagtcgtt tctttgcaca aagccagtct ctgacccctg ctcccggctg gcagtctctc
     1201 gggtccagcc agagccggct gggctccctc gactgttccc actcattctc cagccggacc
     1261 gatctcaatc actggaatgg tgctgggctg tcgggaacta actgtggaga ccttcacggc
     1321 acttcactct gggggacccc gcattattcc acaagcctgt ggggtccccc aagcagcagc
     1381 gacccccgag gaattagcag cccagctccc attaacgctt ttctttctgt tgaccacctg
     1441 ggtgggggtg gagagtccat gtaa
//
