LOCUS       sequence-440677-        1252 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression GST fusion CD28 CAR hinge (I114-P152) in Escherichia 
            coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Folimonova V, Chen X, Negi H, Schwieters CD, Li J, Byrd RA, Taylor 
            N, Youkharibache P, Walters KJ
  TITLE     CD28 hinge used in chimeric antigen receptor (CAR) T-cells exhibits 
            local structure and conformational exchange amidst global disorder.
  JOURNAL   Commun Biol. 2024 Aug 31;7(1):1072. doi: 10.1038/s42003-024-06770-w.
  PUBMED    39217198
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-08-31"; volume: "7(1):1072. doi"; pages: " 
            10.1038/s42003-024-06770-w"
COMMENT     Sequence Label: pGEX6P1-CD28 CAR hinge (I114 – P152)
FEATURES             Location/Qualifiers
     source          1..1252
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(223..245)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(390..408)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        476..580
                     /gene="bla"
                     /label=AmpR promoter
     CDS             765..1144
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(799..818)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cggatctgga gttctgttcc aggggcccct gggatccata gaagtaatgt accccccacc
       61 ttatctagac aacgagaaaa gcaatggcac cattatccat gttaagggta agcacctgtg
      121 cccgtccccg ttgttcccgg gtccaagcaa accgtaactc gagcggccgc atcgtgactg
      181 actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
      241 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
      301 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
      361 cggagtgtat aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
      421 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
      481 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      541 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      601 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      661 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      721 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      781 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
      841 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      901 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
      961 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     1021 cgcttttttg cacaacatgg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1081 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     1141 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1201 actggatgga ggcggataaa gttgcaggac ccttctgcgc ccggcccttc cg
//
