LOCUS       sequence-440688-       15019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expressino of the N. nambi HISPS gene under the PNOS 
            promoter and the constitutive expression of EGFP and p19..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15019)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 15019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4807
FEATURES             Location/Qualifiers
     source          1..15019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..262
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      5417..5669
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        6674..7018
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     6962..6982
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(7092..7094,7095..7097,7098..7808)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7092..7094,7095..7097,7098..7808)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7137..7158)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7398..7417)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7745..7766
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      7844..8096
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8338..8362
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9663..10292
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10721..11794
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11860..12054
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12290..12312)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12398..12538
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12453..12472
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12553..12570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12724..13312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12804..12823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13400..14191)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    14977..15001
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagatacat gagaattaag ggagtcacgg tatgaccccc gccgatgacg cgggacaagc
       61 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactgag ccgcgggttt
      121 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
      181 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
      241 aattcccctc ggtatccaat tagagtctca tattcactct caactcgatc gaggcaatga
      301 actcaagtaa aaatcctcct tctactctcc ttgatgtttt cttggatacc gcaagaaact
      361 tggatacagc aagcagaaat gtgctcgagt gtggtgaaca tcgatggagt tacagagaac
      421 tggatacagt gtcctcagct ctcgcccagc atcttcgata cacggtgggg ctgtccccta
      481 ctgtcgcagt gatcagcgag aatcacccgt acatattggc cctcatgctc gctgtatgga
      541 aattgggcgg taccttcgct ccgatagatg tccacagccc tgcagaactg gttgccggga
      601 tgcttaacat agtctcaccg tcttgcctgg tcatcccgtc atcagacgta acaaaccaga
      661 cccttgcatg tgatcttaac attccagtgg ttgcatttca tcctcaccaa agcacaatcc
      721 cggaactgaa caaaaagtac ctgacggaca gtcaaatcag ccctgatctc ccattccccg
      781 atcccaaccg tccggctctt tacctgttca cttcttctgc tacgagtaga tccaacttga
      841 agtgcgtgcc cctgacccac accttcatac tgagaaatag cctgtcaaag agggcctggt
      901 gcaaaagaat gaggccagaa acagatttcg atggtataag ggttttagga tgggcaccat
      961 ggagtcatgt tctggctcac atgcaagata tagggcccct tacactcctg aatgctggat
     1021 gttacgtctt tgcaacgaca ccgagcacat atccaaccga gttgaaggac gatagggatg
     1081 tcatttcttg cgccgcaaat gcagttatgt acaagggggt taagtccttc gcctgtctcc
     1141 cgtttgtgct gggaggcctt aaggccctgt gtgagtccga gccgtccgtc aaagcacaat
     1201 tacaggtcga ggagcgagca cagttgttaa agagcttaca acacatgaag attcttgaat
     1261 gtggtggcgc tatgctcgaa gtttcagtgg cttcttgggc tatcgagaat cgtattccca
     1321 ttagtatagg aatcggtatg accgaaactg gcggagcctt gttcgctgga cccgttcaag
     1381 caatccaaac gggatttagt tcagaggaca agtttattga ggatgccaca tatctccttg
     1441 tcaaagacga ttacgagtca cacgcagaag aggacatcaa tgaaggcgaa ctggttgtta
     1501 aaagccgaat gctgccaagg ggttatctgg ggtataacga tccatccttc tcagtggacg
     1561 atgcagggtg ggttaccttc aaaacaggcg acaggtactc cgtgaccccg gatggtaagt
     1621 tttcttggtt aggaaggaat acagacttta tacagatgac aagcggtgag actctcgatc
     1681 ccaggcccat cgaaagtttg ctttgcgagt cctcccttat atcaagagcc tgtgtaatag
     1741 gcgataagtt cctgaatggg cccgccacag cagtgtgtgc cataatagaa ttggagccga
     1801 ctacggtaga gaaaggtcag gcccatagca gggacatagc ccgaattttt gcccccataa
     1861 accgtgattt gcctcctcct ttaagaatcg cctggagcca cgtcctcgtg ctccaaccca
     1921 gtgaaaaaat accgatgacc aaaaaaggaa cgatcttccg taagaagatc gaacaagtct
     1981 ttggtagtgc acttggtggg tcctcaggtg acaattccca agcaaccact gatgcatcag
     2041 tcgtccgtcg agatgaactg tcaaacactg ttaagcatat catatcacgt gttctgggcg
     2101 tgagcgacga cgagttgctg tggactctct catttgccga acttggaatg acctccgccc
     2161 ttgcaacgag aattgccaat gaattgaatg aggttttagt aggggtcaat ctgcctatca
     2221 atgcatgtta tatacacgtt gacttaccat ccctgtcaaa tgcagtatat gccaaactgg
     2281 ctcacttgaa actccctgac agaacaccag agccgcgtaa ggcaccggtt gagaatcctg
     2341 gcggaaaaga gatcgtaata gtgggacaag catttagact tccaggctct attaatgacg
     2401 tggctagtct ccgtgacgct tttctggccc gtcaagcaag ttcaatcatt acagaaatac
     2461 caccagacag atgggatcac gcatccttct acccgaagga catacgtttc aacaaggccg
     2521 gcttagtgga cattgctaac tacgaccaca gtttcttcgg cttgacggct acagaagctc
     2581 tttacttatc tccgaccatg agactcgccc tggaagttag ttttgaggcc cttgagaacg
     2641 caaacattcc ggtttctcag ctgaaggggt cacaaacagc tgtctacgtt gctaccacgg
     2701 atgacgggtt cgagactctc ttaaatgcag aggctgggta cgacgcttac acccgattct
     2761 acggtacggg gcgtgcagct tccacggcat ccggcaggat ctcatacctg cttgacgttc
     2821 atggacccag tataactgtg gacacggcat gtagtggagg ggctgtttgc attgatcaag
     2881 ctatagacta cctgcaatcc agttcagccg ctgataccgc catcatttgt gcttctaata
     2941 ctcattgctg gccaggatca ttcatgtttc tgagcgccca gggtatggta agctccggcg
     3001 gacgatgcgc tactttcacg accgatgccg acggctacgt acctagcgag ggtgcagtcg
     3061 ccttcattct taaaacacgt gaagcagcta tgagggacaa agacacaatt ctggctacca
     3121 tcaaagcaac acaaatttct cataatggta ggtcccaggg attagtcgcc cccaatgtaa
     3181 attcccaagc cgacttgcat agaagcctgc tccaaaaagc cgggctttca ccggccgaca
     3241 ttcatttcat cgaggcacac ggaactggaa cctccctcgg agatctgtcc gagatccaag
     3301 ctataaatga tgcatatacg agctcacaac ctcgaacggc cggcccgctg atcgtttctg
     3361 caagcaagac agtgatagga cacactgaac cagccggtcc gttggtgggg atgctgtcag
     3421 tactgaactc ctttaaagag ggggccgtcc ctggtttggc acacttgacc gccgataacc
     3481 tgaatcccgc tcttgattgc agcagtgttc cgttactcat accttaccag cccgttcacc
     3541 tggcagcccc caagcctcat cgtgctgctg taatgtcata tggattctct ggcaccctcg
     3601 gtgggatcgt gctggaggca ccagatgagg aaagactcga agaggaacct cctaatgaca
     3661 aacctatgtt gtttgtagtt agtgcaaaaa cacatactgc attaatagaa tatctcggcc
     3721 gataccttga attcctcttg caggcaaatc cacaagactt ctgcgatatt tgttatactt
     3781 catgcgttgg gcgtgaacat tatcgataca gattcgcatg cgtagccaac gacatggagg
     3841 atttaatagg acaattgcag aagcgtttag gctctaaggt cccaccgaaa ccttcctaca
     3901 aaagaggggc ccttgcattc gccttcagcg gtcagggcac acagttccga gggatggcta
     3961 ccgagctcgc aaaagcctat tccggattcc gaaaaatagt ctctgatctc gctaagaggg
     4021 caagtgaact ctcaggtcac gctattgatc gatttttgtt agcttacgat attggggctg
     4081 aaaacgtcgc cccggatagc gaggctgatc aaatatgtat cttcgtatat caatgtagcg
     4141 tgcttcgttg gcttcagaca atgggcatta gaccatctgc tgttataggg cactccctcg
     4201 gtgagattag cgcttctgtc gccgccggtg cattgtctct ggactctgca ctggaccttg
     4261 ttatcagtcg agctcgactg ttaaggagct ctaccaatgc acccgccggt atggcagcca
     4321 tgtcagcatc acaggacgaa gttgttgagt tgattgggaa attagacctt gataaagcaa
     4381 attcactctc tgtttctgtt atcaatggtc ctcagaatac ggtagtaagc ggttcttcag
     4441 cagccattga aagtatagtc gcactggcca aagggcgaaa gataaaagca agtgctctca
     4501 acatcaacca agccttccac tctccctacg tagattctgc agtaccaggt ttgcgtgctt
     4561 ggtcagagaa gcatatttcc tctgctagac cattacagat ccccctgtac tctacactcc
     4621 ttggcgccca agtaagtgag ggacaaatgc tgaatccgga tcattgggta gatcacgcaa
     4681 gaaaacctgt ccaattcgct caggccgcca ccattatgaa agaaagcttt accggagtaa
     4741 tcattgacat aggtcctcaa gtagttgctt ggagtttgct gctctcaaac ggactgacat
     4801 cagttaccgc tctcgccgca aagcgaggac gatcacagca ggttgccttc cttagtgctc
     4861 ttgcagattt ataccaagac tatggtgtcg tgcctgactt tgtgggatta tacgctcaac
     4921 aggaggacgc cagtcgttta aagaaaaccg atattctgac ctatcctttc caaagggtga
     4981 gaaggtatcc atctttcatc ccgagtcgta gggcacctac tcatgcccat gtccaagatg
     5041 aggaaacatt gtcatctgga tcttccacac ccacattgga gaatacagat ctcgactccg
     5101 gtaaagagtc tttgatggga cccacacgtg gccttcttcg agtagacgat ctgagagaca
     5161 gtatcgtttc ttctgtaaag gatgtactgg agctgaaatc aaatgaggac ttggacttgt
     5221 ccgagtcctt gaacgcattg ggaatggaca gtattatgtt cgctcaatta cgtaaaagga
     5281 taggggaggg actcgggtta tccgtcccga tggtattcct gtctgacgcc ttctcaatcg
     5341 gcgagatggt tagtaacctt gtagaacagg cagaggctag tgaggacaac tgagcttgga
     5401 atggatcttc gatcccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     5461 tgttgccggt cttgcgacga ttatcatata atttctgttg aattacgtta agcatgtaat
     5521 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     5581 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     5641 gcgcgcggtg tcatctatgt tactagatcg ggaattgcca agctaattct tgaagacgaa
     5701 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5761 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5821 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatggg
     5881 accgactcgc gctgtcagga gctcaatggt aactttactc tttatttaac catacatttt
     5941 tttttatttt tttcactttg ttcttcatcc actattgttc tttgttcatc ttgaacaaaa
     6001 gctccctcct tctttgttct tcatccacca ttgttcttca tcaatcattt cgctgtcagg
     6061 agactagagc caagctgatc tcctttgccc cggagatcac catggacgac tttctctatc
     6121 tctacgatct aggaagaaag ttcgacggag aaggtgacga taccatgttc accaccgata
     6181 atgagaagat tagcctcttc aatttcagaa agaatgctga cccacagatg gttagagagg
     6241 cctacgcggc aggtctgatc aagacgatct acccgagtaa taatctccag gagatcaaat
     6301 accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca
     6361 cagagaaaga tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct
     6421 tgcttcataa accaaggcaa gtaatagaga ttggagtctc taagaaagta gttcctactg
     6481 aatcaaaggc catggagtca aaaattcaga tcgaggatct aacagaactc gccgtgaaga
     6541 ctggcgaaca gttcatacag agtcttttac gactcaatga caagaagaaa atcttcgtca
     6601 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     6661 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     6721 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca
     6781 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcccctgcc gacagtggtc
     6841 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     6901 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     6961 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggactc
     7021 cggtattttt acaacaatta ccacaacaaa acaaacaaca aacaacatta caatttacta
     7081 ttctagtcga aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     7141 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     7201 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     7261 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     7321 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     7381 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     7441 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     7501 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     7561 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     7621 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     7681 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     7741 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     7801 tgtacaagga cgagctgtaa gcttggaatg gatcttcgat cccgatcgtt caaacatttg
     7861 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgacgatta tcatataatt
     7921 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     7981 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     8041 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     8101 attgccaagc taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     8161 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     8221 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8281 aaccctgata aatgcttcaa taatgggacc gactcgcgct gtcatgagac cggatcctga
     8341 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
     8401 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     8461 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
     8521 ctgacgttca gtgcagccgt catctgaaaa cgacatgtcg cacaagtcct aagttacgcg
     8581 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
     8641 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
     8701 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
     8761 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
     8821 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac
     8881 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca
     8941 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc
     9001 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat
     9061 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg
     9121 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg
     9181 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact
     9241 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga
     9301 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc
     9361 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg
     9421 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acctctcaac cgtgcggctg
     9481 catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc
     9541 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg
     9601 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac
     9661 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa
     9721 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc
     9781 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg
     9841 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag
     9901 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact
     9961 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg
    10021 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt
    10081 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg
    10141 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg
    10201 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc
    10261 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa
    10321 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt
    10381 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga
    10441 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc
    10501 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt
    10561 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat
    10621 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc
    10681 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc
    10741 cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc
    10801 gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc
    10861 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat
    10921 taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt
    10981 gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga
    11041 ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc
    11101 agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca
    11161 tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt
    11221 ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa
    11281 agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac
    11341 gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg
    11401 ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga
    11461 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga
    11521 ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc
    11581 aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg
    11641 agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga
    11701 gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa
    11761 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat
    11821 tgccctagca ggggaaaaag gtcgaaaagg actctttcct gtggatagca cgtacattgg
    11881 gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat
    11941 tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc
    12001 ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc
    12061 tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct
    12121 acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct
    12181 acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg
    12241 cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    12301 tgcagctccc ggtgacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    12361 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
    12421 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
    12481 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    12541 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    12601 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    12661 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    12721 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    12781 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    12841 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    12901 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    12961 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    13021 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    13081 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    13141 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    13201 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    13261 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    13321 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    13381 ggtcatgcat tctaggtgat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
    13441 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
    13501 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
    13561 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
    13621 acaacgtaag cactacattt cgctcatcac cagcccagtc gggcggcgag ttccatagcg
    13681 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
    13741 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
    13801 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
    13861 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
    13921 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
    13981 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
    14041 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
    14101 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
    14161 tcgatacttc ggcgatcacc gcttccctca taatgtttaa ctttgtttta gggcgactgc
    14221 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
    14281 cttgctgctt ggatgcccga ggcatagact gtaccccaaa aaaacagtca taacaagcca
    14341 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
    14401 gtgagcgcat acgctacttg cattacagct tacgaaccga acaggcttat gtccactggg
    14461 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggt agcagcgaag
    14521 tcgaggcatt tctgtcctgg ctggaacaga acttattatt tccttcctct tttctacagt
    14581 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
    14641 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
    14701 ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct
    14761 gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag
    14821 cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac
    14881 ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt
    14941 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
    15001 cataacggat ccggtctca
//
