LOCUS       sequence-440690-       23653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the copper and PhiC31 regulated expression of HispS and 
            the constitutive expression of CPH, LUZ, H3H and eGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 23653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4655
FEATURES             Location/Qualifiers
     source          1..23653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      362..1068
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1077..1110
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     terminator      6283..6535
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7541..7885
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     RBS             8013..8021
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      8903..9155
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        10161..10505
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(10875..10886)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      11406..11658
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        12502..12846
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      14212..14464
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        15308..15652
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(15726..15728,15729..15731,15732..16442)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(15726..15728,15729..15731,15732..16442)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(15771..15792)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(16032..16051)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     16379..16400
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      16478..16730
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    16972..16996
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             18297..18926
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             19355..20428
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      20494..20688
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(20924..20946)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    21032..21172
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     21087..21106
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(21187..21204)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(21358..21946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(21438..21457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(22034..22825)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    23611..23635
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagagtggt cttgggagag cgatgcgtct tttccgatga accgttccag caaaaaagac
       61 taagcgatgc gtcttttccg ctgaaccgtt ccagcaaaaa agactaagcg atgcgtcttt
      121 tccgctgaac cgttccagca aaaaagacta ggagagcgat gcgtcttttc cgctgaaccg
      181 ttccagcaaa aaagactatc ccgtcctaat acctctacgt gcaagcagta taaaaacaaa
      241 atacataaac ataactaaaa tctcatatcg ttgtaattgt catatactag tggtaattaa
      301 gtccagttac acttgtgccc caactggggt aacctttgag ttctctcagt tgggggcgta
      361 gctgctttaa tgagatatgc gagaagccta tgatcgcatg atatttgctt tcaattctgt
      421 tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt
      481 cattctaatg aatatatcac cgttactatc gtatttttat gaataatatt ctccgttcaa
      541 tttactgatt gtaccctact acttatatgt acaatattaa aatgaaaaca atatattgtg
      601 ctgaataggt ttatagcgac atctatgata gagcgccaca ataacaaaca attgcgtttt
      661 attattacaa atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt
      721 acataaatct tattcaaatt tcaaaagtgc cccaggggct agtatctacg acacaccgag
      781 cggcgaacta ataacgctca ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga
      841 gattccttga agttgagtat tggccgtccg ctctaccgaa agttacgggc accattcaac
      901 ccggtccagc acggcggccg ggtaaccgac ttgctgcccc gagaattatg cagcattttt
      961 ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt
     1021 tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg ctcagcagcg gtgcgggtgc
     1081 cagggcgtgc ccttgggctc cccgggcgcg tactccacaa ggttaaattt gctgactctt
     1141 ttttaccttt catttctgaa aaatgaactc aagtaaaaat cctccttcta ctctccttga
     1201 tgttttcttg gataccgcaa gaaacttgga tacagcaagc agaaatgtgc tcgagtgtgg
     1261 tgaacatcga tggagttaca gagaactgga tacagtgtcc tcagctctcg cccagcatct
     1321 tcgatacacg gtggggctgt cccctactgt cgcagtgatc agcgagaatc acccgtacat
     1381 attggccctc atgctcgctg tatggaaatt gggcggtacc ttcgctccga tagatgtcca
     1441 cagccctgca gaactggttg ccgggatgct taacatagtc tcaccgtctt gcctggtcat
     1501 cccgtcatca gacgtaacaa accagaccct tgcatgtgat cttaacattc cagtggttgc
     1561 atttcatcct caccaaagca caatcccgga actgaacaaa aagtacctga cggacagtca
     1621 aatcagccct gatctcccat tccccgatcc caaccgtccg gctctttacc tgttcacttc
     1681 ttctgctacg agtagatcca acttgaagtg cgtgcccctg acccacacct tcatactgag
     1741 aaatagcctg tcaaagaggg cctggtgcaa aagaatgagg ccagaaacag atttcgatgg
     1801 tataagggtt ttaggatggg caccatggag tcatgttctg gctcacatgc aagatatagg
     1861 gccccttaca ctcctgaatg ctggatgtta cgtctttgca acgacaccga gcacatatcc
     1921 aaccgagttg aaggacgata gggatgtcat ttcttgcgcc gcaaatgcag ttatgtacaa
     1981 gggggttaag tccttcgcct gtctcccgtt tgtgctggga ggccttaagg ccctgtgtga
     2041 gtccgagccg tccgtcaaag cacaattaca ggtcgaggag cgagcacagt tgttaaagag
     2101 cttacaacac atgaagattc ttgaatgtgg tggcgctatg ctcgaagttt cagtggcttc
     2161 ttgggctatc gagaatcgta ttcccattag tataggaatc ggtatgaccg aaactggcgg
     2221 agccttgttc gctggacccg ttcaagcaat ccaaacggga tttagttcag aggacaagtt
     2281 tattgaggat gccacatatc tccttgtcaa agacgattac gagtcacacg cagaagagga
     2341 catcaatgaa ggcgaactgg ttgttaaaag ccgaatgctg ccaaggggtt atctggggta
     2401 taacgatcca tccttctcag tggacgatgc agggtgggtt accttcaaaa caggcgacag
     2461 gtactccgtg accccggatg gtaagttttc ttggttagga aggaatacag actttataca
     2521 gatgacaagc ggtgagactc tcgatcccag gcccatcgaa agtttgcttt gcgagtcctc
     2581 ccttatatca agagcctgtg taataggcga taagttcctg aatgggcccg ccacagcagt
     2641 gtgtgccata atagaattgg agccgactac ggtagagaaa ggtcaggccc atagcaggga
     2701 catagcccga atttttgccc ccataaaccg tgatttgcct cctcctttaa gaatcgcctg
     2761 gagccacgtc ctcgtgctcc aacccagtga aaaaataccg atgaccaaaa aaggaacgat
     2821 cttccgtaag aagatcgaac aagtctttgg tagtgcactt ggtgggtcct caggtgacaa
     2881 ttcccaagca accactgatg catcagtcgt ccgtcgagat gaactgtcaa acactgttaa
     2941 gcatatcata tcacgtgttc tgggcgtgag cgacgacgag ttgctgtgga ctctctcatt
     3001 tgccgaactt ggaatgacct ccgcccttgc aacgagaatt gccaatgaat tgaatgaggt
     3061 tttagtaggg gtcaatctgc ctatcaatgc atgttatata cacgttgact taccatccct
     3121 gtcaaatgca gtatatgcca aactggctca cttgaaactc cctgacagaa caccagagcc
     3181 gcgtaaggca ccggttgaga atcctggcgg aaaagagatc gtaatagtgg gacaagcatt
     3241 tagacttcca ggctctatta atgacgtggc tagtctccgt gacgcttttc tggcccgtca
     3301 agcaagttca atcattacag aaataccacc agacagatgg gatcacgcat ccttctaccc
     3361 gaaggacata cgtttcaaca aggccggctt agtggacatt gctaactacg accacagttt
     3421 cttcggcttg acggctacag aagctcttta cttatctccg accatgagac tcgccctgga
     3481 agttagtttt gaggcccttg agaacgcaaa cattccggtt tctcagctga aggggtcaca
     3541 aacagctgtc tacgttgcta ccacggatga cgggttcgag actctcttaa atgcagaggc
     3601 tgggtacgac gcttacaccc gattctacgg tacggggcgt gcagcttcca cggcatccgg
     3661 caggatctca tacctgcttg acgttcatgg acccagtata actgtggaca cggcatgtag
     3721 tggaggggct gtttgcattg atcaagctat agactacctg caatccagtt cagccgctga
     3781 taccgccatc atttgtgctt ctaatactca ttgctggcca ggatcattca tgtttctgag
     3841 cgcccagggt atggtaagct ccggcggacg atgcgctact ttcacgaccg atgccgacgg
     3901 ctacgtacct agcgagggtg cagtcgcctt cattcttaaa acacgtgaag cagctatgag
     3961 ggacaaagac acaattctgg ctaccatcaa agcaacacaa atttctcata atggtaggtc
     4021 ccagggatta gtcgccccca atgtaaattc ccaagccgac ttgcatagaa gcctgctcca
     4081 aaaagccggg ctttcaccgg ccgacattca tttcatcgag gcacacggaa ctggaacctc
     4141 cctcggagat ctgtccgaga tccaagctat aaatgatgca tatacgagct cacaacctcg
     4201 aacggccggc ccgctgatcg tttctgcaag caagacagtg ataggacaca ctgaaccagc
     4261 cggtccgttg gtggggatgc tgtcagtact gaactccttt aaagaggggg ccgtccctgg
     4321 tttggcacac ttgaccgccg ataacctgaa tcccgctctt gattgcagca gtgttccgtt
     4381 actcatacct taccagcccg ttcacctggc agcccccaag cctcatcgtg ctgctgtaat
     4441 gtcatatgga ttctctggca ccctcggtgg gatcgtgctg gaggcaccag atgaggaaag
     4501 actcgaagag gaacctccta atgacaaacc tatgttgttt gtagttagtg caaaaacaca
     4561 tactgcatta atagaatatc tcggccgata ccttgaattc ctcttgcagg caaatccaca
     4621 agacttctgc gatatttgtt atacttcatg cgttgggcgt gaacattatc gatacagatt
     4681 cgcatgcgta gccaacgaca tggaggattt aataggacaa ttgcagaagc gtttaggctc
     4741 taaggtccca ccgaaacctt cctacaaaag aggggccctt gcattcgcct tcagcggtca
     4801 gggcacacag ttccgaggga tggctaccga gctcgcaaaa gcctattccg gattccgaaa
     4861 aatagtctct gatctcgcta agagggcaag tgaactctca ggtcacgcta ttgatcgatt
     4921 tttgttagct tacgatattg gggctgaaaa cgtcgccccg gatagcgagg ctgatcaaat
     4981 atgtatcttc gtatatcaat gtagcgtgct tcgttggctt cagacaatgg gcattagacc
     5041 atctgctgtt atagggcact ccctcggtga gattagcgct tctgtcgccg ccggtgcatt
     5101 gtctctggac tctgcactgg accttgttat cagtcgagct cgactgttaa ggagctctac
     5161 caatgcaccc gccggtatgg cagccatgtc agcatcacag gacgaagttg ttgagttgat
     5221 tgggaaatta gaccttgata aagcaaattc actctctgtt tctgttatca atggtcctca
     5281 gaatacggta gtaagcggtt cttcagcagc cattgaaagt atagtcgcac tggccaaagg
     5341 gcgaaagata aaagcaagtg ctctcaacat caaccaagcc ttccactctc cctacgtaga
     5401 ttctgcagta ccaggtttgc gtgcttggtc agagaagcat atttcctctg ctagaccatt
     5461 acagatcccc ctgtactcta cactccttgg cgcccaagta agtgagggac aaatgctgaa
     5521 tccggatcat tgggtagatc acgcaagaaa acctgtccaa ttcgctcagg ccgccaccat
     5581 tatgaaagaa agctttaccg gagtaatcat tgacataggt cctcaagtag ttgcttggag
     5641 tttgctgctc tcaaacggac tgacatcagt taccgctctc gccgcaaagc gaggacgatc
     5701 acagcaggtt gccttcctta gtgctcttgc agatttatac caagactatg gtgtcgtgcc
     5761 tgactttgtg ggattatacg ctcaacagga ggacgccagt cgtttaaaga aaaccgatat
     5821 tctgacctat cctttccaaa gggtgagaag gtatccatct ttcatcccga gtcgtagggc
     5881 acctactcat gcccatgtcc aagatgagga aacattgtca tctggatctt ccacacccac
     5941 attggagaat acagatctcg actccggtaa agagtctttg atgggaccca cacgtggcct
     6001 tcttcgagta gacgatctga gagacagtat cgtttcttct gtaaaggatg tactggagct
     6061 gaaatcaaat gaggacttgg acttgtccga gtccttgaac gcattgggaa tggacagtat
     6121 tatgttcgct caattacgta aaaggatagg ggagggactc gggttatccg tcccgatggt
     6181 attcctgtct gacgccttct caatcggcga gatggttagt aaccttgtag aacaggcaga
     6241 ggctagtgag gacaactgag cttggaatgg atcttcgatc ccgatcgttc aaacatttgg
     6301 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgacgattat catataattt
     6361 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     6421 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     6481 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     6541 ttgccaagct aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     6601 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     6661 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6721 accctgataa atgcttcaat aatgggaccg actcgcgctg tcaggagtct caatggtaac
     6781 tttactcttt atttaaccat acattttttt ttattttttt cactttgttc ttcatccact
     6841 attgttcttt gttcatcttg aacaaaagct ccctccttct ttgttcttca tccaccattg
     6901 ttcttcatca atcatttcgc tgtcaggaga ctagagccaa gctgatctcc tttgccccgg
     6961 agatcaccat ggacgacttt ctctatctct acgatctagg aagaaagttc gacggagaag
     7021 gtgacgatac catgttcacc accgataatg agaagattag cctcttcaat ttcagaaaga
     7081 atgctgaccc acagatggtt agagaggcct acgcggcagg tctgatcaag acgatctacc
     7141 cgagtaataa tctccaggag atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa
     7201 gattcaggac taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta
     7261 ctattccagt atggacgatt caaggcttgc ttcataaacc aaggcaagta atagagattg
     7321 gagtctctaa gaaagtagtt cctactgaat caaaggccat ggagtcaaaa attcagatcg
     7381 aggatctaac agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac
     7441 tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctc gtctactcca
     7501 agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
     7561 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga
     7621 cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg
     7681 ttcaagatgc ccctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     7741 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
     7801 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
     7861 gaagttcatt tcatttggag aggactccgg tatttttaca acaattacca caacaaaaca
     7921 aacaacaaac aacattacaa tttactattc tagtcgaaat ggccccaata agttccacgt
     7981 ggtctaggct cataaggttt gtggctgtgg aaacctcctt agttcacatc ggcgagccta
     8041 ttgatgcaac aatggacgtg ggcttagcac gtagggaagg gaagacaata caagcctatg
     8101 agatcatcgg cagtggctct gctttagatc tgtctgccca agtgtcaaag aacgtactta
     8161 cggtccgtga gctcttaatg cccttgagcc gtgaggagat taaaacggta agatgccttg
     8221 gccttaatta tcccgtccac gccacagaag ccaacgtggc cgtgccgaaa ttcccgaacc
     8281 ttttttacaa acccgtaacc tccctcatcg gaccaggtgg gttgataacc atcccttcag
     8341 ttgtccaacc acctaaagag caccagagcg attacgaagc cgagttggtt atcgtcatcg
     8401 gtaaagccgc caaaaatgtc agtgaagatg aagctctgga ctacgtgttg ggctacaccg
     8461 cagccaatga catctcattt aggaaacatc agttggccgt ttctcaatgg tcattctcca
     8521 agggttttga tggcacgaat cccctgggac cgtgcctggt ttctgcttcc tctattccag
     8581 atccacagga tattccgatc caatgtaaac tcaacggtgg ggtggtgcaa aatggaaaca
     8641 ctcgtgatca gatttttaat gtcaagaaaa caatctcttt tttaagccaa gggactacgt
     8701 tggagcctgg ttccataatc ctgacgggga cgcccgacgg agtgggcttc gtaagaaacc
     8761 caccgcttta tttgaaggac ggtgatgaag taatgacttg gatcggatca ggtataggaa
     8821 ccttagccaa cactgtccga gaggagcaga cttgtttcgc ttcaggaggg cacgagtaag
     8881 cttggaatgg atcttcgatc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     8941 gaatcctgtt gccggtcttg cgacgattat catataattt ctgttgaatt acgttaagca
     9001 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     9061 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     9121 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttgccaagct aattcttgaa
     9181 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     9241 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     9301 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9361 aatgggaccg actcgcgctg tcaggagtct caatggtaac tttactcttt atttaaccat
     9421 acattttttt ttattttttt cactttgttc ttcatccact attgttcttt gttcatcttg
     9481 aacaaaagct ccctccttct ttgttcttca tccaccattg ttcttcatca atcatttcgc
     9541 tgtcaggaga ctagagccaa gctgatctcc tttgccccgg agatcaccat ggacgacttt
     9601 ctctatctct acgatctagg aagaaagttc gacggagaag gtgacgatac catgttcacc
     9661 accgataatg agaagattag cctcttcaat ttcagaaaga atgctgaccc acagatggtt
     9721 agagaggcct acgcggcagg tctgatcaag acgatctacc cgagtaataa tctccaggag
     9781 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
     9841 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
     9901 caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa gaaagtagtt
     9961 cctactgaat caaaggccat ggagtcaaaa attcagatcg aggatctaac agaactcgcc
    10021 gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa gaagaaaatc
    10081 ttcgtcaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc
    10141 tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc
    10201 ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc
    10261 acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ccctgccgac
    10321 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
    10381 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
    10441 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
    10501 aggactccgg tatttttaca acaattacca caacaaaaca aacaacaaac aacattacaa
    10561 tttactattc tagtcgaaat gagaattaac atctcccttt catcattatt tgaacgtctg
    10621 tcaaagttat ccagcagatc cattgcaatc acctgtggtg tcgtcttggc ctccgcaatc
    10681 gcttttccga taatccgtag ggactatcag acttttttgg aagtcgggcc gagttacgct
    10741 ccgcagaatt tcagaggtta catcatagta tgcgtgcttt cactctttag gcaggaacaa
    10801 aaagggttgg ctatctatga tcgactgccc gaaaagcgaa ggtggttggc agatctccca
    10861 ttccgagaag gtacgcgtcc ttctatcaca agtcatatca tccaaagaca gaggactcag
    10921 ctggtagacc aagaatttgc aacccgagaa ctcattgata aagttatacc cagagttcag
    10981 gctcgacaca ccgataagac gtttctgtca acctcaaagt tcgaatttca tgccaaagca
    11041 atttttttgc tgcccagtat tcccatcaat gatcccttga atataccttc acatgatact
    11101 gtcagacgaa caaaaaggga gatcgctcac atgcacgatt accacgactg caccctgcac
    11161 ttggctctcg cagcccagga cggtaaagaa gtacttaaaa aagggtgggg ccagagacat
    11221 cccctcgctg gccccggcgt gccgggacct ccaaccgaat ggacgttttt atatgcaccg
    11281 cgtaacgagg aggaagccag agtggtcgag atgatagtgg aggccagtat tggttacatg
    11341 acaaatgacc ccgctggtaa aattgtagag aacgctaagt gagcttggaa tggatcttcg
    11401 atcccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
    11461 ttgcgacgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
    11521 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
    11581 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
    11641 catctatgtt actagatcgg gaattgccaa gctaattctt gaagacgaaa gggcctcgtg
    11701 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    11761 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    11821 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatggga ccgactcgcg
    11881 ctgtcaggag actagagcca agctgatctc ctttgccccg gagatcacca tggacgactt
    11941 tctctatctc tacgatctag gaagaaagtt cgacggagaa ggtgacgata ccatgttcac
    12001 caccgataat gagaagatta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
    12061 tagagaggcc tacgcggcag gtctgatcaa gacgatctac ccgagtaata atctccagga
    12121 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat
    12181 caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat
    12241 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta agaaagtagt
    12301 tcctactgaa tcaaaggcca tggagtcaaa aattcagatc gaggatctaa cagaactcgc
    12361 cgtgaagact ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat
    12421 cttcgtcaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
    12481 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
    12541 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
    12601 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cccctgccga
    12661 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
    12721 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
    12781 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
    12841 gaggactccg gtatttttac aacaattacc acaacaaaac aaacaacaaa caacattaca
    12901 atttactatt ctagtcgaaa tggcatcctt tgaaaatagt ctgtccgttt tgatcgtcgg
    12961 ggctggcctt gggggattag cagccgcaat agctcttagg agacagggcc atgtagtaaa
    13021 gatctatgat agttcctcat tcaaagctga actgggtgct ggccttgctg tacctccgaa
    13081 tacgcttaga agccttcaac agttaggttg taatacagag aatttgaacg gagtcgacaa
    13141 tctctgcttc acggccatgg gttacgacgg ctccgtaggt atgatgaata acatgacaga
    13201 ctacagggag gcttatggca catcctggat tatggttcat cgagtggatt tacataatga
    13261 acttatgcgt gtcgcacttg atccgggggg gctgggacca ccagcaacat tacatttaaa
    13321 tcacagagtg accttctgcg acgttgacgc atgtacggta acttttacca acgggacgac
    13381 acaaagtgcc gacttgatcg taggggctga cggcatacga agcaccatac gtagattcgt
    13441 cttggaggaa gatgtaactg tgcctgccag cgggatcgtc ggtttcagat ggctggtgca
    13501 agccgatgcc ttagaccctt accctgagtt agattggatt gttaagaagc ctccactcgg
    13561 tgctcgtctg atctctacgc cacagaatcc acagtccggc gtggggttgg ctgaccgtcg
    13621 tacaattatc atctacgctt gtaggggtgg cacgatggtt aatgtgttgg ctgtccacga
    13681 cgacgaaaga gatcagaata cggcagattg gtcagtgccg gcttccaaag atgatctctt
    13741 ccgtgtattc catgactacc acccaagatt cagacgtttg ctcgaacttg ctcaagatat
    13801 caatctctgg caaatgaggg tagttcccgt gcttaaaaaa tgggtgaaca agcgtgtctg
    13861 tttattagga gatgccgccc atgcttctct ccccacgctg ggtcaagggt tcggaatggg
    13921 actggaggac gccgtggcac tcggcacatt gttacccaaa ggaactacgg cttcacagat
    13981 cgaaacgaga ctggccgttt acgagcaatt acgaaaggat agggctgagt tcgtagcagc
    14041 agaatcctac gaggaacagt atgtccctga gatgcgtggg ctctatcttc gttccaaaga
    14101 actccgagat cgagtgatgg gatatgatat taaagtggag tcagaaaagg ttttggagac
    14161 tctgcttcga tcctctaatt ctgcatgagc ttggaatgga tcttcgatcc cgatcgttca
    14221 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gacgattatc
    14281 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
    14341 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
    14401 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
    14461 gatcgggaat tgccaagcta attcttgaag acgaaagggc ctcgtgatac gcctattttt
    14521 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
    14581 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    14641 gagacaataa ccctgataaa tgcttcaata atgggaccga ctcgcgctgt caggagacta
    14701 gagccaagct gatctccttt gccccggaga tcaccatgga cgactttctc tatctctacg
    14761 atctaggaag aaagttcgac ggagaaggtg acgataccat gttcaccacc gataatgaga
    14821 agattagcct cttcaatttc agaaagaatg ctgacccaca gatggttaga gaggcctacg
    14881 cggcaggtct gatcaagacg atctacccga gtaataatct ccaggagatc aaataccttc
    14941 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
    15001 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
    15061 ataaaccaag gcaagtaata gagattggag tctctaagaa agtagttcct actgaatcaa
    15121 aggccatgga gtcaaaaatt cagatcgagg atctaacaga actcgccgtg aagactggcg
    15181 aacagttcat acagagtctt ttacgactca atgacaagaa gaaaatcttc gtcaacatgg
    15241 tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa
    15301 gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc
    15361 cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc
    15421 atcattgcga taaaggaaag gctatcgttc aagatgcccc tgccgacagt ggtcccaaag
    15481 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
    15541 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
    15601 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg actccggtat
    15661 ttttacaaca attaccacaa caaaacaaac aacaaacaac attacaattt actattctag
    15721 tcgaaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
    15781 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
    15841 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
    15901 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
    15961 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
    16021 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
    16081 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
    16141 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
    16201 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
    16261 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
    16321 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
    16381 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
    16441 aggacgagct gtaagcttgg aatggatctt cgatcccgat cgttcaaaca tttggcaata
    16501 aagtttctta agattgaatc ctgttgccgg tcttgcgacg attatcatat aatttctgtt
    16561 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
    16621 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
    16681 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattgcc
    16741 aagctaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
    16801 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
    16861 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    16921 gataaatgct tcaataatgg gaccgactcg cgctgtcatg agaccggatc ctgacaggat
    16981 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
    17041 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
    17101 tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
    17161 ttcagtgcag ccgtcatctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
    17221 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
    17281 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
    17341 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
    17401 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
    17461 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
    17521 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
    17581 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
    17641 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
    17701 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac
    17761 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
    17821 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
    17881 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
    17941 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa
    18001 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg
    18061 atcgcggccg ggtacgtgtt cgagccgccc gcgcacctct caaccgtgcg gctgcatgaa
    18121 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
    18181 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
    18241 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
    18301 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
    18361 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
    18421 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
    18481 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
    18541 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
    18601 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
    18661 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
    18721 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
    18781 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
    18841 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
    18901 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
    18961 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
    19021 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
    19081 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
    19141 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
    19201 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
    19261 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca
    19321 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc
    19381 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
    19441 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
    19501 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
    19561 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
    19621 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
    19681 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
    19741 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
    19801 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
    19861 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
    19921 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
    19981 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
    20041 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
    20101 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
    20161 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
    20221 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
    20281 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
    20341 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
    20401 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
    20461 agcaggggaa aaaggtcgaa aaggactctt tcctgtggat agcacgtaca ttgggaaccc
    20521 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
    20581 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
    20641 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
    20701 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
    20761 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
    20821 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca
    20881 tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    20941 tcccggtgac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    21001 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
    21061 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
    21121 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
    21181 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    21241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    21301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    21361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    21421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    21481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    21541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    21601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    21661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    21721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    21781 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    21841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    21901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    21961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    22021 gcattctagg tgattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa
    22081 attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct
    22141 gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg
    22201 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
    22261 taagcactac atttcgctca tcaccagccc agtcgggcgg cgagttccat agcgttaagg
    22321 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
    22381 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
    22441 tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa
    22501 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg
    22561 tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt
    22621 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
    22681 caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
    22741 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
    22801 cttcggcgat caccgcttcc ctcataatgt ttaactttgt tttagggcga ctgccctgct
    22861 gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct
    22921 gcttggatgc ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa
    22981 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc
    23041 gcatacgcta cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg
    23101 ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggtagcagc gaagtcgagg
    23161 catttctgtc ctggctggaa cagaacttat tatttccttc ctcttttcta cagtatttaa
    23221 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
    23281 aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
    23341 tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc
    23401 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
    23461 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
    23521 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
    23581 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacataac
    23641 ggatccggtc tca
//
