LOCUS       sequence-440694-       22823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expression of the N. nambi HISPS gene under the 
            regulation of the Red light-regulated Etr4:mDFR promoter and TNOS in
            and the constitutve expression of CPH, LUZ, H3H, EGFP and p19..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22823)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 22823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4541
FEATURES             Location/Qualifiers
     source          1..22823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5453..5705
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        6711..7055
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     RBS             7183..7191
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      8073..8325
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        9331..9675
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(10045..10056)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      10576..10828
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        11672..12016
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      13382..13634
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        14478..14822
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(14896..14898,14899..14901,14902..15612)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(14896..14898,14899..14901,14902..15612)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(14941..14962)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(15202..15221)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     15549..15570
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      15648..15900
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    16142..16166
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             17467..18096
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             18525..19598
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      19664..19858
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(20094..20116)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    20202..20342
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     20257..20276
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(20357..20374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(20528..21116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(20608..20627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(21204..21995)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    22781..22805
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagaaaagc ttcgaagttt aaacgattga atataaccga cgtgactgtt acatttaggg
       61 attgaatata accgacgtga ctgttacatt tagggattga atataaccga cgtgactgtt
      121 acatttaggg attgaatata accgacgtga ctgttacatt tagggatgct agctcccgtc
      181 ctaatacctc tacgtgcaag cagtataaaa acaaaataca taaacataac taaaatctca
      241 tatcgttgta attgtcatat actagtggta attaagtcca gttacactta aggttaaatt
      301 tgctgactct tttttacctt tcatttctga aaatgaactc aagtaaaaat cctccttcta
      361 ctctccttga tgttttcttg gataccgcaa gaaacttgga tacagcaagc agaaatgtgc
      421 tcgagtgtgg tgaacatcga tggagttaca gagaactgga tacagtgtcc tcagctctcg
      481 cccagcatct tcgatacacg gtggggctgt cccctactgt cgcagtgatc agcgagaatc
      541 acccgtacat attggccctc atgctcgctg tatggaaatt gggcggtacc ttcgctccga
      601 tagatgtcca cagccctgca gaactggttg ccgggatgct taacatagtc tcaccgtctt
      661 gcctggtcat cccgtcatca gacgtaacaa accagaccct tgcatgtgat cttaacattc
      721 cagtggttgc atttcatcct caccaaagca caatcccgga actgaacaaa aagtacctga
      781 cggacagtca aatcagccct gatctcccat tccccgatcc caaccgtccg gctctttacc
      841 tgttcacttc ttctgctacg agtagatcca acttgaagtg cgtgcccctg acccacacct
      901 tcatactgag aaatagcctg tcaaagaggg cctggtgcaa aagaatgagg ccagaaacag
      961 atttcgatgg tataagggtt ttaggatggg caccatggag tcatgttctg gctcacatgc
     1021 aagatatagg gccccttaca ctcctgaatg ctggatgtta cgtctttgca acgacaccga
     1081 gcacatatcc aaccgagttg aaggacgata gggatgtcat ttcttgcgcc gcaaatgcag
     1141 ttatgtacaa gggggttaag tccttcgcct gtctcccgtt tgtgctggga ggccttaagg
     1201 ccctgtgtga gtccgagccg tccgtcaaag cacaattaca ggtcgaggag cgagcacagt
     1261 tgttaaagag cttacaacac atgaagattc ttgaatgtgg tggcgctatg ctcgaagttt
     1321 cagtggcttc ttgggctatc gagaatcgta ttcccattag tataggaatc ggtatgaccg
     1381 aaactggcgg agccttgttc gctggacccg ttcaagcaat ccaaacggga tttagttcag
     1441 aggacaagtt tattgaggat gccacatatc tccttgtcaa agacgattac gagtcacacg
     1501 cagaagagga catcaatgaa ggcgaactgg ttgttaaaag ccgaatgctg ccaaggggtt
     1561 atctggggta taacgatcca tccttctcag tggacgatgc agggtgggtt accttcaaaa
     1621 caggcgacag gtactccgtg accccggatg gtaagttttc ttggttagga aggaatacag
     1681 actttataca gatgacaagc ggtgagactc tcgatcccag gcccatcgaa agtttgcttt
     1741 gcgagtcctc ccttatatca agagcctgtg taataggcga taagttcctg aatgggcccg
     1801 ccacagcagt gtgtgccata atagaattgg agccgactac ggtagagaaa ggtcaggccc
     1861 atagcaggga catagcccga atttttgccc ccataaaccg tgatttgcct cctcctttaa
     1921 gaatcgcctg gagccacgtc ctcgtgctcc aacccagtga aaaaataccg atgaccaaaa
     1981 aaggaacgat cttccgtaag aagatcgaac aagtctttgg tagtgcactt ggtgggtcct
     2041 caggtgacaa ttcccaagca accactgatg catcagtcgt ccgtcgagat gaactgtcaa
     2101 acactgttaa gcatatcata tcacgtgttc tgggcgtgag cgacgacgag ttgctgtgga
     2161 ctctctcatt tgccgaactt ggaatgacct ccgcccttgc aacgagaatt gccaatgaat
     2221 tgaatgaggt tttagtaggg gtcaatctgc ctatcaatgc atgttatata cacgttgact
     2281 taccatccct gtcaaatgca gtatatgcca aactggctca cttgaaactc cctgacagaa
     2341 caccagagcc gcgtaaggca ccggttgaga atcctggcgg aaaagagatc gtaatagtgg
     2401 gacaagcatt tagacttcca ggctctatta atgacgtggc tagtctccgt gacgcttttc
     2461 tggcccgtca agcaagttca atcattacag aaataccacc agacagatgg gatcacgcat
     2521 ccttctaccc gaaggacata cgtttcaaca aggccggctt agtggacatt gctaactacg
     2581 accacagttt cttcggcttg acggctacag aagctcttta cttatctccg accatgagac
     2641 tcgccctgga agttagtttt gaggcccttg agaacgcaaa cattccggtt tctcagctga
     2701 aggggtcaca aacagctgtc tacgttgcta ccacggatga cgggttcgag actctcttaa
     2761 atgcagaggc tgggtacgac gcttacaccc gattctacgg tacggggcgt gcagcttcca
     2821 cggcatccgg caggatctca tacctgcttg acgttcatgg acccagtata actgtggaca
     2881 cggcatgtag tggaggggct gtttgcattg atcaagctat agactacctg caatccagtt
     2941 cagccgctga taccgccatc atttgtgctt ctaatactca ttgctggcca ggatcattca
     3001 tgtttctgag cgcccagggt atggtaagct ccggcggacg atgcgctact ttcacgaccg
     3061 atgccgacgg ctacgtacct agcgagggtg cagtcgcctt cattcttaaa acacgtgaag
     3121 cagctatgag ggacaaagac acaattctgg ctaccatcaa agcaacacaa atttctcata
     3181 atggtaggtc ccagggatta gtcgccccca atgtaaattc ccaagccgac ttgcatagaa
     3241 gcctgctcca aaaagccggg ctttcaccgg ccgacattca tttcatcgag gcacacggaa
     3301 ctggaacctc cctcggagat ctgtccgaga tccaagctat aaatgatgca tatacgagct
     3361 cacaacctcg aacggccggc ccgctgatcg tttctgcaag caagacagtg ataggacaca
     3421 ctgaaccagc cggtccgttg gtggggatgc tgtcagtact gaactccttt aaagaggggg
     3481 ccgtccctgg tttggcacac ttgaccgccg ataacctgaa tcccgctctt gattgcagca
     3541 gtgttccgtt actcatacct taccagcccg ttcacctggc agcccccaag cctcatcgtg
     3601 ctgctgtaat gtcatatgga ttctctggca ccctcggtgg gatcgtgctg gaggcaccag
     3661 atgaggaaag actcgaagag gaacctccta atgacaaacc tatgttgttt gtagttagtg
     3721 caaaaacaca tactgcatta atagaatatc tcggccgata ccttgaattc ctcttgcagg
     3781 caaatccaca agacttctgc gatatttgtt atacttcatg cgttgggcgt gaacattatc
     3841 gatacagatt cgcatgcgta gccaacgaca tggaggattt aataggacaa ttgcagaagc
     3901 gtttaggctc taaggtccca ccgaaacctt cctacaaaag aggggccctt gcattcgcct
     3961 tcagcggtca gggcacacag ttccgaggga tggctaccga gctcgcaaaa gcctattccg
     4021 gattccgaaa aatagtctct gatctcgcta agagggcaag tgaactctca ggtcacgcta
     4081 ttgatcgatt tttgttagct tacgatattg gggctgaaaa cgtcgccccg gatagcgagg
     4141 ctgatcaaat atgtatcttc gtatatcaat gtagcgtgct tcgttggctt cagacaatgg
     4201 gcattagacc atctgctgtt atagggcact ccctcggtga gattagcgct tctgtcgccg
     4261 ccggtgcatt gtctctggac tctgcactgg accttgttat cagtcgagct cgactgttaa
     4321 ggagctctac caatgcaccc gccggtatgg cagccatgtc agcatcacag gacgaagttg
     4381 ttgagttgat tgggaaatta gaccttgata aagcaaattc actctctgtt tctgttatca
     4441 atggtcctca gaatacggta gtaagcggtt cttcagcagc cattgaaagt atagtcgcac
     4501 tggccaaagg gcgaaagata aaagcaagtg ctctcaacat caaccaagcc ttccactctc
     4561 cctacgtaga ttctgcagta ccaggtttgc gtgcttggtc agagaagcat atttcctctg
     4621 ctagaccatt acagatcccc ctgtactcta cactccttgg cgcccaagta agtgagggac
     4681 aaatgctgaa tccggatcat tgggtagatc acgcaagaaa acctgtccaa ttcgctcagg
     4741 ccgccaccat tatgaaagaa agctttaccg gagtaatcat tgacataggt cctcaagtag
     4801 ttgcttggag tttgctgctc tcaaacggac tgacatcagt taccgctctc gccgcaaagc
     4861 gaggacgatc acagcaggtt gccttcctta gtgctcttgc agatttatac caagactatg
     4921 gtgtcgtgcc tgactttgtg ggattatacg ctcaacagga ggacgccagt cgtttaaaga
     4981 aaaccgatat tctgacctat cctttccaaa gggtgagaag gtatccatct ttcatcccga
     5041 gtcgtagggc acctactcat gcccatgtcc aagatgagga aacattgtca tctggatctt
     5101 ccacacccac attggagaat acagatctcg actccggtaa agagtctttg atgggaccca
     5161 cacgtggcct tcttcgagta gacgatctga gagacagtat cgtttcttct gtaaaggatg
     5221 tactggagct gaaatcaaat gaggacttgg acttgtccga gtccttgaac gcattgggaa
     5281 tggacagtat tatgttcgct caattacgta aaaggatagg ggagggactc gggttatccg
     5341 tcccgatggt attcctgtct gacgccttct caatcggcga gatggttagt aaccttgtag
     5401 aacaggcaga ggctagtgag gacaactgag cttggaatgg atcttcgatc ccgatcgttc
     5461 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgacgattat
     5521 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     5581 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     5641 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     5701 agatcgggaa ttgccaagct aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     5761 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     5821 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5881 tgagacaata accctgataa atgcttcaat aatgggaccg actcgcgctg tcaggagtct
     5941 caatggtaac tttactcttt atttaaccat acattttttt ttattttttt cactttgttc
     6001 ttcatccact attgttcttt gttcatcttg aacaaaagct ccctccttct ttgttcttca
     6061 tccaccattg ttcttcatca atcatttcgc tgtcaggaga ctagagccaa gctgatctcc
     6121 tttgccccgg agatcaccat ggacgacttt ctctatctct acgatctagg aagaaagttc
     6181 gacggagaag gtgacgatac catgttcacc accgataatg agaagattag cctcttcaat
     6241 ttcagaaaga atgctgaccc acagatggtt agagaggcct acgcggcagg tctgatcaag
     6301 acgatctacc cgagtaataa tctccaggag atcaaatacc ttcccaagaa ggttaaagat
     6361 gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat atttctcaag
     6421 atcagaagta ctattccagt atggacgatt caaggcttgc ttcataaacc aaggcaagta
     6481 atagagattg gagtctctaa gaaagtagtt cctactgaat caaaggccat ggagtcaaaa
     6541 attcagatcg aggatctaac agaactcgcc gtgaagactg gcgaacagtt catacagagt
     6601 cttttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctc
     6661 gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt
     6721 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     6781 atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga
     6841 aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc cccacccacg
     6901 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     6961 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     7021 tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca acaattacca
     7081 caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgaaat ggccccaata
     7141 agttccacgt ggtctaggct cataaggttt gtggctgtgg aaacctcctt agttcacatc
     7201 ggcgagccta ttgatgcaac aatggacgtg ggcttagcac gtagggaagg gaagacaata
     7261 caagcctatg agatcatcgg cagtggctct gctttagatc tgtctgccca agtgtcaaag
     7321 aacgtactta cggtccgtga gctcttaatg cccttgagcc gtgaggagat taaaacggta
     7381 agatgccttg gccttaatta tcccgtccac gccacagaag ccaacgtggc cgtgccgaaa
     7441 ttcccgaacc ttttttacaa acccgtaacc tccctcatcg gaccaggtgg gttgataacc
     7501 atcccttcag ttgtccaacc acctaaagag caccagagcg attacgaagc cgagttggtt
     7561 atcgtcatcg gtaaagccgc caaaaatgtc agtgaagatg aagctctgga ctacgtgttg
     7621 ggctacaccg cagccaatga catctcattt aggaaacatc agttggccgt ttctcaatgg
     7681 tcattctcca agggttttga tggcacgaat cccctgggac cgtgcctggt ttctgcttcc
     7741 tctattccag atccacagga tattccgatc caatgtaaac tcaacggtgg ggtggtgcaa
     7801 aatggaaaca ctcgtgatca gatttttaat gtcaagaaaa caatctcttt tttaagccaa
     7861 gggactacgt tggagcctgg ttccataatc ctgacgggga cgcccgacgg agtgggcttc
     7921 gtaagaaacc caccgcttta tttgaaggac ggtgatgaag taatgacttg gatcggatca
     7981 ggtataggaa ccttagccaa cactgtccga gaggagcaga cttgtttcgc ttcaggaggg
     8041 cacgagtaag cttggaatgg atcttcgatc ccgatcgttc aaacatttgg caataaagtt
     8101 tcttaagatt gaatcctgtt gccggtcttg cgacgattat catataattt ctgttgaatt
     8161 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     8221 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     8281 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttgccaagct
     8341 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     8401 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8461 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8521 atgcttcaat aatgggaccg actcgcgctg tcaggagtct caatggtaac tttactcttt
     8581 atttaaccat acattttttt ttattttttt cactttgttc ttcatccact attgttcttt
     8641 gttcatcttg aacaaaagct ccctccttct ttgttcttca tccaccattg ttcttcatca
     8701 atcatttcgc tgtcaggaga ctagagccaa gctgatctcc tttgccccgg agatcaccat
     8761 ggacgacttt ctctatctct acgatctagg aagaaagttc gacggagaag gtgacgatac
     8821 catgttcacc accgataatg agaagattag cctcttcaat ttcagaaaga atgctgaccc
     8881 acagatggtt agagaggcct acgcggcagg tctgatcaag acgatctacc cgagtaataa
     8941 tctccaggag atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac
     9001 taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt
     9061 atggacgatt caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa
     9121 gaaagtagtt cctactgaat caaaggccat ggagtcaaaa attcagatcg aggatctaac
     9181 agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa
     9241 gaagaaaatc ttcgtcaaca tggtggagca cgacactctc gtctactcca agaatatcaa
     9301 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg
     9361 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
     9421 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
     9481 ccctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     9541 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     9601 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     9661 tcatttggag aggactccgg tatttttaca acaattacca caacaaaaca aacaacaaac
     9721 aacattacaa tttactattc tagtcgaaat gagaattaac atctcccttt catcattatt
     9781 tgaacgtctg tcaaagttat ccagcagatc cattgcaatc acctgtggtg tcgtcttggc
     9841 ctccgcaatc gcttttccga taatccgtag ggactatcag acttttttgg aagtcgggcc
     9901 gagttacgct ccgcagaatt tcagaggtta catcatagta tgcgtgcttt cactctttag
     9961 gcaggaacaa aaagggttgg ctatctatga tcgactgccc gaaaagcgaa ggtggttggc
    10021 agatctccca ttccgagaag gtacgcgtcc ttctatcaca agtcatatca tccaaagaca
    10081 gaggactcag ctggtagacc aagaatttgc aacccgagaa ctcattgata aagttatacc
    10141 cagagttcag gctcgacaca ccgataagac gtttctgtca acctcaaagt tcgaatttca
    10201 tgccaaagca atttttttgc tgcccagtat tcccatcaat gatcccttga atataccttc
    10261 acatgatact gtcagacgaa caaaaaggga gatcgctcac atgcacgatt accacgactg
    10321 caccctgcac ttggctctcg cagcccagga cggtaaagaa gtacttaaaa aagggtgggg
    10381 ccagagacat cccctcgctg gccccggcgt gccgggacct ccaaccgaat ggacgttttt
    10441 atatgcaccg cgtaacgagg aggaagccag agtggtcgag atgatagtgg aggccagtat
    10501 tggttacatg acaaatgacc ccgctggtaa aattgtagag aacgctaagt gagcttggaa
    10561 tggatcttcg atcccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
    10621 gttgccggtc ttgcgacgat tatcatataa tttctgttga attacgttaa gcatgtaata
    10681 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
    10741 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
    10801 cgcgcggtgt catctatgtt actagatcgg gaattgccaa gctaattctt gaagacgaaa
    10861 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
    10921 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    10981 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatggga
    11041 ccgactcgcg ctgtcaggag actagagcca agctgatctc ctttgccccg gagatcacca
    11101 tggacgactt tctctatctc tacgatctag gaagaaagtt cgacggagaa ggtgacgata
    11161 ccatgttcac caccgataat gagaagatta gcctcttcaa tttcagaaag aatgctgacc
    11221 cacagatggt tagagaggcc tacgcggcag gtctgatcaa gacgatctac ccgagtaata
    11281 atctccagga gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
    11341 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
    11401 tatggacgat tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta
    11461 agaaagtagt tcctactgaa tcaaaggcca tggagtcaaa aattcagatc gaggatctaa
    11521 cagaactcgc cgtgaagact ggcgaacagt tcatacagag tcttttacga ctcaatgaca
    11581 agaagaaaat cttcgtcaac atggtggagc acgacactct cgtctactcc aagaatatca
    11641 aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg
    11701 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
    11761 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
    11821 cccctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
    11881 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
    11941 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
    12001 ttcatttgga gaggactccg gtatttttac aacaattacc acaacaaaac aaacaacaaa
    12061 caacattaca atttactatt ctagtcgaaa tggcatcctt tgaaaatagt ctgtccgttt
    12121 tgatcgtcgg ggctggcctt gggggattag cagccgcaat agctcttagg agacagggcc
    12181 atgtagtaaa gatctatgat agttcctcat tcaaagctga actgggtgct ggccttgctg
    12241 tacctccgaa tacgcttaga agccttcaac agttaggttg taatacagag aatttgaacg
    12301 gagtcgacaa tctctgcttc acggccatgg gttacgacgg ctccgtaggt atgatgaata
    12361 acatgacaga ctacagggag gcttatggca catcctggat tatggttcat cgagtggatt
    12421 tacataatga acttatgcgt gtcgcacttg atccgggggg gctgggacca ccagcaacat
    12481 tacatttaaa tcacagagtg accttctgcg acgttgacgc atgtacggta acttttacca
    12541 acgggacgac acaaagtgcc gacttgatcg taggggctga cggcatacga agcaccatac
    12601 gtagattcgt cttggaggaa gatgtaactg tgcctgccag cgggatcgtc ggtttcagat
    12661 ggctggtgca agccgatgcc ttagaccctt accctgagtt agattggatt gttaagaagc
    12721 ctccactcgg tgctcgtctg atctctacgc cacagaatcc acagtccggc gtggggttgg
    12781 ctgaccgtcg tacaattatc atctacgctt gtaggggtgg cacgatggtt aatgtgttgg
    12841 ctgtccacga cgacgaaaga gatcagaata cggcagattg gtcagtgccg gcttccaaag
    12901 atgatctctt ccgtgtattc catgactacc acccaagatt cagacgtttg ctcgaacttg
    12961 ctcaagatat caatctctgg caaatgaggg tagttcccgt gcttaaaaaa tgggtgaaca
    13021 agcgtgtctg tttattagga gatgccgccc atgcttctct ccccacgctg ggtcaagggt
    13081 tcggaatggg actggaggac gccgtggcac tcggcacatt gttacccaaa ggaactacgg
    13141 cttcacagat cgaaacgaga ctggccgttt acgagcaatt acgaaaggat agggctgagt
    13201 tcgtagcagc agaatcctac gaggaacagt atgtccctga gatgcgtggg ctctatcttc
    13261 gttccaaaga actccgagat cgagtgatgg gatatgatat taaagtggag tcagaaaagg
    13321 ttttggagac tctgcttcga tcctctaatt ctgcatgagc ttggaatgga tcttcgatcc
    13381 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
    13441 gacgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
    13501 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
    13561 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
    13621 tatgttacta gatcgggaat tgccaagcta attcttgaag acgaaagggc ctcgtgatac
    13681 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
    13741 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    13801 atccgctcat gagacaataa ccctgataaa tgcttcaata atgggaccga ctcgcgctgt
    13861 caggagacta gagccaagct gatctccttt gccccggaga tcaccatgga cgactttctc
    13921 tatctctacg atctaggaag aaagttcgac ggagaaggtg acgataccat gttcaccacc
    13981 gataatgaga agattagcct cttcaatttc agaaagaatg ctgacccaca gatggttaga
    14041 gaggcctacg cggcaggtct gatcaagacg atctacccga gtaataatct ccaggagatc
    14101 aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag
    14161 aacacagaga aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa
    14221 ggcttgcttc ataaaccaag gcaagtaata gagattggag tctctaagaa agtagttcct
    14281 actgaatcaa aggccatgga gtcaaaaatt cagatcgagg atctaacaga actcgccgtg
    14341 aagactggcg aacagttcat acagagtctt ttacgactca atgacaagaa gaaaatcttc
    14401 gtcaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
    14461 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
    14521 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
    14581 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcccc tgccgacagt
    14641 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
    14701 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
    14761 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
    14821 actccggtat ttttacaaca attaccacaa caaaacaaac aacaaacaac attacaattt
    14881 actattctag tcgaaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
    14941 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
    15001 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
    15061 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
    15121 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
    15181 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
    15241 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
    15301 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
    15361 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
    15421 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
    15481 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
    15541 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
    15601 gagctgtaca aggacgagct gtaagcttgg aatggatctt cgatcccgat cgttcaaaca
    15661 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgacg attatcatat
    15721 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
    15781 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
    15841 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
    15901 gggaattgcc aagctaattc ttgaagacga aagggcctcg tgatacgcct atttttatag
    15961 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
    16021 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
    16081 caataaccct gataaatgct tcaataatgg gaccgactcg cgctgtcatg agaccggatc
    16141 ctgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
    16201 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
    16261 agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg
    16321 gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat gtcgcacaag tcctaagtta
    16381 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
    16441 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
    16501 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
    16561 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
    16621 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
    16681 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
    16741 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
    16801 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
    16861 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
    16921 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
    16981 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
    17041 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
    17101 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
    17161 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
    17221 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacctct caaccgtgcg
    17281 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
    17341 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
    17401 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
    17461 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
    17521 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
    17581 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
    17641 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
    17701 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
    17761 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
    17821 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
    17881 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
    17941 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
    18001 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
    18061 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
    18121 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
    18181 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
    18241 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
    18301 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
    18361 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
    18421 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc
    18481 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
    18541 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
    18601 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
    18661 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
    18721 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
    18781 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
    18841 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
    18901 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
    18961 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
    19021 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
    19081 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
    19141 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
    19201 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
    19261 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
    19321 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
    19381 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
    19441 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
    19501 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
    19561 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
    19621 aaattgccct agcaggggaa aaaggtcgaa aaggactctt tcctgtggat agcacgtaca
    19681 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
    19741 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
    19801 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
    19861 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
    19921 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
    19981 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
    20041 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
    20101 cacatgcagc tcccggtgac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    20161 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
    20221 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
    20281 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    20341 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    20401 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    20461 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    20521 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    20581 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    20641 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    20701 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    20761 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    20821 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    20881 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    20941 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    21001 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    21061 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    21121 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    21181 ttttggtcat gcattctagg tgattatttg ccgactacct tggtgatctc gcctttcacg
    21241 tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca
    21301 agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt
    21361 gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg
    21421 cgggacaacg taagcactac atttcgctca tcaccagccc agtcgggcgg cgagttccat
    21481 agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt
    21541 tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata
    21601 gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc
    21661 cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc
    21721 acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt
    21781 tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta
    21841 accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa
    21901 tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt
    21961 tgagtcgata cttcggcgat caccgcttcc ctcataatgt ttaactttgt tttagggcga
    22021 ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa
    22081 cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca gtcataacaa
    22141 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
    22201 ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc ttatgtccac
    22261 tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggtagcagc
    22321 gaagtcgagg catttctgtc ctggctggaa cagaacttat tatttccttc ctcttttcta
    22381 cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc
    22441 cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg
    22501 gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg
    22561 ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg
    22621 gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta
    22681 caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt
    22741 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg
    22801 taaacataac ggatccggtc tca
//
