LOCUS       sequence-440842-       12647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human KIF1Bß in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12647)
  TITLE     Characterizing Human KIF1Bß Motor Activity by Single-Molecule 
            Motility Assays and Caenorhabtidis elegans Genetics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSN721
FEATURES             Location/Qualifiers
     source          1..12647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..38
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    155..176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(191..196,197..214,215..221)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    229..245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     234..256
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     253..269
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     253..269
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(3237..3251)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(10481..10503)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10603..10622
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10704..10722)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10790..10894
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10895..10963,10964..11755)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11113..11132)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11926..12514
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12415..12434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
       61 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      121 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      181 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      241 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca tgcggtttta
      301 gaagaaattt cgatgggagt tgatgacatt tgtgattttt aaacttaaat tttaacttat
      361 cgcttagtga tctttaatca taaccgactt tagcaagttc atgaaaaaag aaactttcga
      421 ccggcattcg atcataattt ttagacatat tatagagcat tctcgtgtct ccccatagtt
      481 gtaaatatgc aaccagatgt ccgttggtgc aatttagttg ctcatcaagt tctctatgtc
      541 catttctatt ctgttcaacc acttcatttc cccttttgac atttttctat ctgtgagagc
      601 ttcatctccc atcgtttcct cttaacgtct tcagaaagac aaatcgttgc caaattcatc
      661 attttcgagt gccaggcatc caataaccga aagaatggga gcaaagagtg cttttcaacg
      721 gaaattagaa ccatttttct gaaagaagtc tgcgcgagat aagcaaagtt ttaaattttc
      781 atgaaaacca caagcgcgta ttttgagttt ttcacaactt tagaaaaatt caaaaaacgg
      841 atgccaacgt tacaggaaaa tgccgaaaat tcgagaaaca ataatttcca tgaacattta
      901 aaaataattt tttcacgacc catttgtaat agtgaattag taagggtatt taatctgata
      961 catttccaaa aaataatgca atgtttgccg aaaacttgac gatttgccgt tcgcctgaaa
     1021 atactgtatg atgactgaaa aatttcccgt ttgcgaaatc tgtgattcct ataaaattag
     1081 acttctaaat aaattggcat ttaaaatcat gattagaaga aaactaaacc acaaagaata
     1141 tgaaaacaca cttctataat taatttttac aattagatat atgatttcat aaagctgcag
     1201 tgctgttatt ttgatctcaa tctatttaca aaaataacac tgaaaagtac tttatgcctt
     1261 gcaagaaatg ctcaatttct gtgtattcta cgcttaagca ccttctattc agcacctact
     1321 cgggaattct ccaccaaatc cattctcatt ttcttctttt ctctctcaac tcgtcttcaa
     1381 tctcttcacc tcgtccacta tttcatcccc ggagacttgt tccccagacg tcgtgcctcc
     1441 aatcgatcca tttgtggagc ccgcgtcgtc cacagagctt ctgccgtttc atctctgcgt
     1501 ctcgttcacc cattagacgt tgtgcttcct ccctgcctga aaatagcccc acacaacata
     1561 gacttgctct tctttcttct actttttttc tcattctttt ttctccagat acgcctttat
     1621 tcaaatgacg tgtttctttt ttttgtctgt tcaaaaacca acaactcatg atcatcagat
     1681 gatgatggtc aaaaatccct tccggttttc cttttcttcc caaattttgg cttggacttt
     1741 ttttgaatta tgacaccaaa acatgcaacg acgtatatat atgcaaacgt agaatatcga
     1801 gtttcagaac cgaatcattg tcgagattaa aagggaggaa attgatatgt gatggaaata
     1861 gattgagggc tgaatttttg agattgaaaa aacaattgta taatgtaagt agataaaaca
     1921 aagctgtatt gaatttataa atatctaata cattttctaa tacaaaaata aatttgattt
     1981 tgtacttttc ttaaaaagtg atcagaattt ccactagatg tttttggcaa caatcacctg
     2041 ataattggat taaaatttga caaaactgtt taagtacaag cgaactagat gagaatttct
     2101 acctttttct ttcaattgaa tattttcgta ttccatccct ccgtgctttc attttccatt
     2161 gacgttttat ggttcaatat atatcctaaa agttttcctt accctttctc ctactagttt
     2221 catctcttct tctaaataat taacatttct ttatttaatt gatttctttc catttcacgt
     2281 catttcttca tttagcacaa cattcttctt ttattcccat ccaatcgcct tctccgttca
     2341 ttccggcccc tttttcagct tcaaattgaa caacaaaaac acattgcgtg acgataaaac
     2401 ttccagttcc cgtcttcaaa acgtcattcc gagagactat tcgcctggaa ccctcgcctt
     2461 tttttcatta tgtgctcatc cctaaaagag ccaagagctc tcaaaaactg ctggggatct
     2521 tcgtgaaaaa atgtttggtt gaaaattgtt gaagcatctt gaattaccat ttactaaagg
     2581 accaagtata cattttgaaa taggctccga aagattgaag catattttga actagataat
     2641 attaattaaa ttcattttgt tggtgttaac ttttatttta agttgcgagc atgttaccgt
     2701 aagaaccttt ttcatttttt attttaaata aaattttaca atttgtcttg agcaacttgt
     2761 tctataagtg atatcaactt caaattagtg ggcatataaa attaaaatga gaaaaaacaa
     2821 tagcgtggat agaaaaatca ccataggcca ggaaaatctg taagagattt tcccgtgtta
     2881 gctaaacttt tgaaattgtt tacattttta aaggttatgt tttaccatct gattctttac
     2941 agatcggttt tttttaacat atgacaatag ttttaacctt tcgtttttga acctttaaat
     3001 ttgaaaatca atagttttca ttaaatactc attgattaca gtagccatta gaactttatg
     3061 tctcctttcg ctgaccttag ggaacattgt gccaaaatca gttttgcttc caaaatcaga
     3121 ttctataatt tctacactgt gactcacaaa aaccaaccaa attcaatcat ttttctgtat
     3181 cctgccttct tcttttttcg aatgttcttt ttctccttgt ttctcttttt tccatgttta
     3241 tcatcgtcat ctgttgcttc ttctcggctt ctctaatttc agcccaaccc cttttttttc
     3301 ctcatctaat taaattcgcc tcgaggctcc gtcctcctcc atctctatct ttttctcttt
     3361 tctcttccat tccgaacttt ttctttccca atcgaccatc tattcgaaga ttcattgctg
     3421 cctttttttc agcactcttc ttcatttttt tttcgaatac acattcaata gaaaactgga
     3481 gaagaagagg aaaagcatcg aggagacgat aagtggggct caacgccatt ggatgattga
     3541 ctcgagctga gaagaaggac tcaagaggtg agccaaaatt gcaaaataga agaagaaaaa
     3601 atagaaaaac aagaggaaag ggaaacaaat gtcttgttat agatgttgat gtgtgaaaaa
     3661 aaacgatata gaaaaatgac atggctgaac ggagttatta tctcgacttg aaaggcggca
     3721 ttcgaaaaat tcagatttta cgttaaaaat tcccttttta actcaaagta cccaaaaatg
     3781 ggctaaaatt caaaaatacc acaatagaac acttaaaaga ttctgtaagt ttgtaggact
     3841 gcactttaat cctaaaaata tggtttcaaa aaaattaatt catgttctga aatgttacta
     3901 aggttttgga aagatcgcaa tgagctttaa cttttcaaga tttttcttca agagttggta
     3961 aaatttttga catcagtaaa cttgtagttt taaaaaactt ttaaaacccc ttttcaattt
     4021 gtttcaaaat ttcaaaatgt tcaaatctta atgcttaaaa ttgaaattca atgaaaatag
     4081 atcacaattc acgtcatttg gcatttgctt cttttccctg cctgagccaa cattttctaa
     4141 tcgaatcaac cactaatctg ttttatttcg tcaatttctt ttttccttct ttccttatta
     4201 gcatatatcg atcttacttc aattgtttaa ataaatctca ataaacaagt tcaaaataac
     4261 atgtgaattc caatttctac gaacgaattc ttgaattaat taaaacaatt tcagggcgcg
     4321 ccatgtcggg agcctcagtg aaggtggctg tccgggtaag gcccttcaat tctcgagaga
     4381 ccagcaagga atccaaatgc atcattcaga tgcaaggcaa ctcgaccagt attattaacc
     4441 caaagaatcc aaaggaagct ccaaagtcct tcagcttcga ctattcctac tggtctcata
     4501 cctcacccga agatccctgt tttgcatctc aaaaccgtgt gtacaatgac attggcaagg
     4561 aaatgctctt acacgccttt gagggatata atgtctgtat ttttgcctat gggcagactg
     4621 gtgctggaaa atcttataca atgatgggta aacaagaaga aagccaggct ggcatcattc
     4681 cacagttatg tgaagaactt tttgagaaaa tcaatgacaa ctgtaatgaa gaaatgtctt
     4741 actctgtaga ggtgagctac atggaaattt actgtgaaag agtacgagat ttgctgaatc
     4801 caaaaaacaa gggtaatttg cgtgtgcgtg aacacccact tcttggaccc tatgtggagg
     4861 atctgtccaa gttggcagtt acttcctaca cagacattgc tgacctcatg gatgctggga
     4921 acaaagccag gacagtggca gctacaaaca tgaatgaaac aagtagccgt tcccacgctg
     4981 tgtttacgat tgttttcacc cagaagaaac acgataatga gaccaacctt tccactgaga
     5041 aggtcagtaa aatcagcttg gtggatctag caggaagtga acgagctgat tcaactggtg
     5101 ccaaagggac tcgattaaag gaaggagcaa atattaataa gtctcttaca actttgggca
     5161 aagtcatttc agccttggcc gaggtgagta aaaagaagaa gaaaacagat tttattccct
     5221 acagggattc tgtacttact tggctccttc gagaaaattt aggtggcaat tctcggactg
     5281 caatggttgc tgctctgagc cccgcggata tcaactacga tgagactttg agcactctga
     5341 gatatgcaga tcgtgcaaaa caaattaaat gcaatgctgt tatcaatgag gaccccaatg
     5401 ccaaactggt tcgtgaatta aaggaggagg tgacacggct gaaggacctt cttcgtgctc
     5461 agggcctggg agatattatt gatacatcca tggggtccct cacttcatcc ccatcttcct
     5521 gctcactcag tagtcaggtg ggcttgacgt ctgtgaccag tattcaagag aggatcatgt
     5581 ctacacctgg aggagaggaa gctattgaac gtttaaagga atcagagaag atcattgctg
     5641 agttgaatga aacttgggaa gagaagcttc gtaaaacaga ggccatcaga atggagagag
     5701 aggctttgtt ggctgagatg ggagttgcca ttcgggaaga tggaggaacc ctaggggttt
     5761 tctcacctaa aaagacccca catcttgtta acctcaatga agacccacta atgtctgagt
     5821 gcctacttta ttacatcaaa gatggaatta caagggttgg ccaagcagat gctgagcggc
     5881 gccaggacat agtgctgagc ggggctcaca ttaaagaaga gcattgtatc ttccggagtg
     5941 agagaagcaa cagcggggaa gttatcgtga ccttagagcc ctgtgagcgc tcagaaacct
     6001 acgtaaatgg caagagggtg tcccagcctg ttcagctgcg ctcaggaaac cgtatcatca
     6061 tgggtaaaaa ccatgttttc cgctttaacc acccggaaca agcacgagct gagcgagaga
     6121 agactccttc tgctgagacc ccctctgagc ctgtggactg gacatttgcc cagagggagc
     6181 ttctggaaaa acaaggaatt gatatgaaac aagagatgga gaaaaggcta caggaaatgg
     6241 agatcttata caaaaaggag aaggaagaag cagatcttct tttggagcag cagagactgg
     6301 actatgagag taaattgcag gccttgcaga agcaggttga aacccgatct ctggctgcag
     6361 aaacaactga agaggaggaa gaagaggaag aagttccttg gacacagcat gaatttgagt
     6421 tggcccaatg ggccttccgg aaatggaagt ctcatcagtt tacttcatta cgggacttac
     6481 tctggggcaa tgccgtgtac ctaaaggagg ccaatgccat cagtgtggaa ctgaaaaaga
     6541 aggtgcagtt tcagtttgtt ctgctgactg acacactgta ctcccctttg cctcctgaat
     6601 tacttcccac tgagatggaa aaaactcatg aggacaggcc tttccctcgc acagtggtag
     6661 cagtagaagt ccaggatttg aagaatggag caacacacta ttggtctttg gagaaactca
     6721 agcagaggct ggatttgatg cgagagatgt atgatagggc aggggagatg gcctccagtg
     6781 cccaagacga aagcgaaacc actgtgactg gcagcgatcc cttctatgat cggttccact
     6841 ggttcaaact tgtggggagc tcccccattt tccacggctg tgtgaacgag cgccttgccg
     6901 accgcacacc ctcccccact ttttccacgg ccgattccga catcactgag ctggctgacg
     6961 agcagcaaga tgagatggag gattttgatg atgaggcatt cgtggatgac gccggctctg
     7021 acgcagggac ggaggaggga tcagatctct tcagtgacgg gcatgacccg ttttacgacc
     7081 gatccccttg gttcatttta gtgggaaggg catttgttta cctgagcaat ctgctgtatc
     7141 ccgtgcccct gatccacagg gtggccatcg tcagtgagaa aggtgaagtg cggggatttc
     7201 tgcgtgtggc tgtacaggcc atcgcagcgg atgaagaagc tcctgattat ggctctggaa
     7261 ttcgacagtc aggaacagct aaaatatctt ttgataatga atactttaat cagagtgact
     7321 tttcgtctgt tgcaatgact cgttctggtc tgtccttgga ggagttgagg attgtggaag
     7381 gacagggtca gagttctgag gtcatcactc ctccagaaga aatcagtcga attaatgact
     7441 tggatttgaa gtcaagcact ttgctggatg gtaagatggt aatggaaggg ttttctgaag
     7501 agattggcaa ccacctgaaa ctgggcagtg ccttcacttt ccgagtaaca gtgttgcagg
     7561 ccagtggaat cctcccagag tatgcagata tcttctgtca gttcaacttt ttgcatcgcc
     7621 atgatgaagc attctccacg gagcccctca aaaacaatgg cagaggaagt cccctggcct
     7681 tttatcatgt gcagaatatt gcagtggaga tcactgaatc atttgtggat tacatcaaaa
     7741 ccaagcctat tgtatttgaa gtctttgggc attatcagca gcacccactt catctgcaag
     7801 gacaggagct taacagtccg cctcagccgt gccgccgatt cttccctcca cccatgccac
     7861 tgtccaagcc agttccagcc accaagttaa acacgatgag caaaaccagc cttggccaga
     7921 gcatgagcaa gtatgacctc ctggtttggt ttgagatcag tgaactggag cctacaggag
     7981 agtatatccc agctgtggtt gaccacacag caggcttgcc ttgccagggg acatttttgc
     8041 ttcatcaggg catccagcga aggatcacag tgaccattat ccatgagaag gggagcgagc
     8101 tccattggaa agatgttcgt gaactggtgg taggtcgtat tcggaataag cctgaggtgg
     8161 atgaagctgc agttgatgcc atcctctccc taaatattat ttctgccaag tacctgaagt
     8221 cttcccacaa ctctagcagg accttctacc gctttgaggc tgtgtgggat agctctctgc
     8281 ataactccct tcttctgaac cgagtgacac cctatggaga aaagatctac atgaccttgt
     8341 cggcctacct agagctggat cattgcatcc agccggctgt catcaccaag gatgtgtgca
     8401 tggtcttcta ctcccgagat gccaagatct caccaccacg ctctctgcgt agcctctttg
     8461 gcagcggcta ctcaaagtca ccagattcga atcgagtcac tggcatttac gaactcagct
     8521 tatgcaaaat gtcagacaca ggtagtccag gtatgcagag aaggagaaga aaaatcttag
     8581 atacgtcagt ggcatatgtg cggggagaag agaacttagc aggctggcgg ccccgtggag
     8641 acagcctcat ccttgagcac cagtgggagc tggagaagct ggagctccta catgaggtgg
     8701 aaaaaacccg ccactttttg ctgctgcgtg agagacttgg tgacagcatc cccaaatccc
     8761 tgagcgactc gttatccccc agcctcagca gtgggaccct cagcacctcc accagtatct
     8821 cctctcagat ctcaaccact acctttgaaa gcgccatcac acctagcgag agcagtggct
     8881 atgattcagg agacatcgaa agcctggtgg accgagagaa agagctggct accaagtgcc
     8941 tgcaacttct cacccacact ttcaacagag aattcagcca ggtgcacggc agcgtcagtg
     9001 actgtaagtt gtctgatatc tctccaattg gacgggatcc ctctgagtcc agtttcagca
     9061 gtgccaccct cactccctcc tccacctgtc cctctctggt agactctagg agcaactctc
     9121 tggatcagaa gaccccagaa gccaattccc gggcctctag tccctgccca gaatttgaac
     9181 agtttcagat tgtcccagct gtggaaacac catatttggc ccgagcagga aaaaacgaat
     9241 ttctcaatct tgttccagat attgaagaaa ttagaccaag ctcagtggtc tctaagaaag
     9301 gataccttca tttcaaggag cctctttaca gtaactgggc taaacatttt gttgtcgtcc
     9361 gtcggcctta tgtcttcatc tataacagtg acaaagaccc tgtggagcgt ggaatcatta
     9421 acctgtccac agcacaggtg gagtacagtg aggaccagca ggccatggtg aagacaccaa
     9481 acacctttgc tgtctgcaca aagcaccgtg gggtcctttt gcaggccctc aatgacaaag
     9541 acatgaacga ctggttgtat gccttcaacc cacttctagc tggcacaata cggtcaaagc
     9601 tttcccgcag atgcccgagc cagtcgaaat actgagtcga cggtaccatg gtattgatat
     9661 ctgagctccg catcggccgc tgtcatcaga tcgccatctc gcgcccgtgc ctctgacttc
     9721 taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
     9781 tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca
     9841 cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa
     9901 aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc
     9961 tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa
    10021 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc
    10081 ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt
    10141 atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt
    10201 ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca
    10261 caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt
    10321 ggaaggtgag tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt
    10381 aaatgacaga atacattccc aatataccaa acataactgt ttctactagt cggccgtacg
    10441 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    10501 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    10561 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    10621 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    10681 gcatcaggcg gccttaatta agggcctcgt gatacgccta tttttatagg ttaatgtcat
    10741 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    10801 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    10861 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    10921 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    10981 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    11041 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    11101 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    11161 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    11221 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    11281 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    11341 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    11401 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    11461 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    11521 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    11581 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    11641 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    11701 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    11761 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    11821 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    11881 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    11941 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    12001 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    12061 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    12121 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    12181 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    12241 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    12301 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    12361 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    12421 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    12481 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    12541 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    12601 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgcc
//
