LOCUS       sequence-440845-       12647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CMT2A mutant version of human KIF1Bß in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12647)
  TITLE     Characterizing Human KIF1Bß Motor Activity by Single-Molecule 
            Motility Assays and Caenorhabtidis elegans Genetics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSN993
FEATURES             Location/Qualifiers
     source          1..12647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..44
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    161..182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(197..202,203..220,221..227)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    235..251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     240..262
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     259..275
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     259..275
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(3243..3257)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(10487..10509)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10609..10628
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10710..10728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10796..10900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10901..10969,10970..11761)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11119..11138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11932..12520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12421..12440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
       61 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      121 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      181 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      241 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatgcg
      301 gttttagaag aaatttcgat gggagttgat gacatttgtg atttttaaac ttaaatttta
      361 acttatcgct tagtgatctt taatcataac cgactttagc aagttcatga aaaaagaaac
      421 tttcgaccgg cattcgatca taatttttag acatattata gagcattctc gtgtctcccc
      481 atagttgtaa atatgcaacc agatgtccgt tggtgcaatt tagttgctca tcaagttctc
      541 tatgtccatt tctattctgt tcaaccactt catttcccct tttgacattt ttctatctgt
      601 gagagcttca tctcccatcg tttcctctta acgtcttcag aaagacaaat cgttgccaaa
      661 ttcatcattt tcgagtgcca ggcatccaat aaccgaaaga atgggagcaa agagtgcttt
      721 tcaacggaaa ttagaaccat ttttctgaaa gaagtctgcg cgagataagc aaagttttaa
      781 attttcatga aaaccacaag cgcgtatttt gagtttttca caactttaga aaaattcaaa
      841 aaacggatgc caacgttaca ggaaaatgcc gaaaattcga gaaacaataa tttccatgaa
      901 catttaaaaa taattttttc acgacccatt tgtaatagtg aattagtaag ggtatttaat
      961 ctgatacatt tccaaaaaat aatgcaatgt ttgccgaaaa cttgacgatt tgccgttcgc
     1021 ctgaaaatac tgtatgatga ctgaaaaatt tcccgtttgc gaaatctgtg attcctataa
     1081 aattagactt ctaaataaat tggcatttaa aatcatgatt agaagaaaac taaaccacaa
     1141 agaatatgaa aacacacttc tataattaat ttttacaatt agatatatga tttcataaag
     1201 ctgcagtgct gttattttga tctcaatcta tttacaaaaa taacactgaa aagtacttta
     1261 tgccttgcaa gaaatgctca atttctgtgt attctacgct taagcacctt ctattcagca
     1321 cctactcggg aattctccac caaatccatt ctcattttct tcttttctct ctcaactcgt
     1381 cttcaatctc ttcacctcgt ccactatttc atccccggag acttgttccc cagacgtcgt
     1441 gcctccaatc gatccatttg tggagcccgc gtcgtccaca gagcttctgc cgtttcatct
     1501 ctgcgtctcg ttcacccatt agacgttgtg cttcctccct gcctgaaaat agccccacac
     1561 aacatagact tgctcttctt tcttctactt tttttctcat tcttttttct ccagatacgc
     1621 ctttattcaa atgacgtgtt tctttttttt gtctgttcaa aaaccaacaa ctcatgatca
     1681 tcagatgatg atggtcaaaa atcccttccg gttttccttt tcttcccaaa ttttggcttg
     1741 gacttttttt gaattatgac accaaaacat gcaacgacgt atatatatgc aaacgtagaa
     1801 tatcgagttt cagaaccgaa tcattgtcga gattaaaagg gaggaaattg atatgtgatg
     1861 gaaatagatt gagggctgaa tttttgagat tgaaaaaaca attgtataat gtaagtagat
     1921 aaaacaaagc tgtattgaat ttataaatat ctaatacatt ttctaataca aaaataaatt
     1981 tgattttgta cttttcttaa aaagtgatca gaatttccac tagatgtttt tggcaacaat
     2041 cacctgataa ttggattaaa atttgacaaa actgtttaag tacaagcgaa ctagatgaga
     2101 atttctacct ttttctttca attgaatatt ttcgtattcc atccctccgt gctttcattt
     2161 tccattgacg ttttatggtt caatatatat cctaaaagtt ttccttaccc tttctcctac
     2221 tagtttcatc tcttcttcta aataattaac atttctttat ttaattgatt tctttccatt
     2281 tcacgtcatt tcttcattta gcacaacatt cttcttttat tcccatccaa tcgccttctc
     2341 cgttcattcc ggcccctttt tcagcttcaa attgaacaac aaaaacacat tgcgtgacga
     2401 taaaacttcc agttcccgtc ttcaaaacgt cattccgaga gactattcgc ctggaaccct
     2461 cgcctttttt tcattatgtg ctcatcccta aaagagccaa gagctctcaa aaactgctgg
     2521 ggatcttcgt gaaaaaatgt ttggttgaaa attgttgaag catcttgaat taccatttac
     2581 taaaggacca agtatacatt ttgaaatagg ctccgaaaga ttgaagcata ttttgaacta
     2641 gataatatta attaaattca ttttgttggt gttaactttt attttaagtt gcgagcatgt
     2701 taccgtaaga acctttttca ttttttattt taaataaaat tttacaattt gtcttgagca
     2761 acttgttcta taagtgatat caacttcaaa ttagtgggca tataaaatta aaatgagaaa
     2821 aaacaatagc gtggatagaa aaatcaccat aggccaggaa aatctgtaag agattttccc
     2881 gtgttagcta aacttttgaa attgtttaca tttttaaagg ttatgtttta ccatctgatt
     2941 ctttacagat cggttttttt taacatatga caatagtttt aacctttcgt ttttgaacct
     3001 ttaaatttga aaatcaatag ttttcattaa atactcattg attacagtag ccattagaac
     3061 tttatgtctc ctttcgctga ccttagggaa cattgtgcca aaatcagttt tgcttccaaa
     3121 atcagattct ataatttcta cactgtgact cacaaaaacc aaccaaattc aatcattttt
     3181 ctgtatcctg ccttcttctt ttttcgaatg ttctttttct ccttgtttct cttttttcca
     3241 tgtttatcat cgtcatctgt tgcttcttct cggcttctct aatttcagcc caaccccttt
     3301 tttttcctca tctaattaaa ttcgcctcga ggctccgtcc tcctccatct ctatcttttt
     3361 ctcttttctc ttccattccg aactttttct ttcccaatcg accatctatt cgaagattca
     3421 ttgctgcctt tttttcagca ctcttcttca tttttttttc gaatacacat tcaatagaaa
     3481 actggagaag aagaggaaaa gcatcgagga gacgataagt ggggctcaac gccattggat
     3541 gattgactcg agctgagaag aaggactcaa gaggtgagcc aaaattgcaa aatagaagaa
     3601 gaaaaaatag aaaaacaaga ggaaagggaa acaaatgtct tgttatagat gttgatgtgt
     3661 gaaaaaaaac gatatagaaa aatgacatgg ctgaacggag ttattatctc gacttgaaag
     3721 gcggcattcg aaaaattcag attttacgtt aaaaattccc tttttaactc aaagtaccca
     3781 aaaatgggct aaaattcaaa aataccacaa tagaacactt aaaagattct gtaagtttgt
     3841 aggactgcac tttaatccta aaaatatggt ttcaaaaaaa ttaattcatg ttctgaaatg
     3901 ttactaaggt tttggaaaga tcgcaatgag ctttaacttt tcaagatttt tcttcaagag
     3961 ttggtaaaat ttttgacatc agtaaacttg tagttttaaa aaacttttaa aacccctttt
     4021 caatttgttt caaaatttca aaatgttcaa atcttaatgc ttaaaattga aattcaatga
     4081 aaatagatca caattcacgt catttggcat ttgcttcttt tccctgcctg agccaacatt
     4141 ttctaatcga atcaaccact aatctgtttt atttcgtcaa tttctttttt ccttctttcc
     4201 ttattagcat atatcgatct tacttcaatt gtttaaataa atctcaataa acaagttcaa
     4261 aataacatgt gaattccaat ttctacgaac gaattcttga attaattaaa acaatttcag
     4321 ggcgcgccat gtcgggagcc tcagtgaagg tggctgtccg ggtaaggccc ttcaattctc
     4381 gagagaccag caaggaatcc aaatgcatca ttcagatgca aggcaactcg accagtatta
     4441 ttaacccaaa gaatccaaag gaagctccaa agtccttcag cttcgactat tcctactggt
     4501 ctcatacctc acccgaagat ccctgttttg catctcaaaa ccgtgtgtac aatgacattg
     4561 gcaaggaaat gctcttacac gcctttgagg gatataatgt ctgtattttt gcctatgggc
     4621 tgactggtgc tggaaaatct tatacaatga tgggtaaaca agaagaaagc caggctggca
     4681 tcattccaca gttatgtgaa gaactttttg agaaaatcaa tgacaactgt aatgaagaaa
     4741 tgtcttactc tgtagaggtg agctacatgg aaatttactg tgaaagagta cgagatttgc
     4801 tgaatccaaa aaacaagggt aatttgcgtg tgcgtgaaca cccacttctt ggaccctatg
     4861 tggaggatct gtccaagttg gcagttactt cctacacaga cattgctgac ctcatggatg
     4921 ctgggaacaa agccaggaca gtggcagcta caaacatgaa tgaaacaagt agccgttccc
     4981 acgctgtgtt tacgattgtt ttcacccaga agaaacacga taatgagacc aacctttcca
     5041 ctgagaaggt cagtaaaatc agcttggtgg atctagcagg aagtgaacga gctgattcaa
     5101 ctggtgccaa agggactcga ttaaaggaag gagcaaatat taataagtct cttacaactt
     5161 tgggcaaagt catttcagcc ttggccgagg tgagtaaaaa gaagaagaaa acagatttta
     5221 ttccctacag ggattctgta cttacttggc tccttcgaga aaatttaggt ggcaattctc
     5281 ggactgcaat ggttgctgct ctgagccccg cggatatcaa ctacgatgag actttgagca
     5341 ctctgagata tgcagatcgt gcaaaacaaa ttaaatgcaa tgctgttatc aatgaggacc
     5401 ccaatgccaa actggttcgt gaattaaagg aggaggtgac acggctgaag gaccttcttc
     5461 gtgctcaggg cctgggagat attattgata catccatggg gtccctcact tcatccccat
     5521 cttcctgctc actcagtagt caggtgggct tgacgtctgt gaccagtatt caagagagga
     5581 tcatgtctac acctggagga gaggaagcta ttgaacgttt aaaggaatca gagaagatca
     5641 ttgctgagtt gaatgaaact tgggaagaga agcttcgtaa aacagaggcc atcagaatgg
     5701 agagagaggc tttgttggct gagatgggag ttgccattcg ggaagatgga ggaaccctag
     5761 gggttttctc acctaaaaag accccacatc ttgttaacct caatgaagac ccactaatgt
     5821 ctgagtgcct actttattac atcaaagatg gaattacaag ggttggccaa gcagatgctg
     5881 agcggcgcca ggacatagtg ctgagcgggg ctcacattaa agaagagcat tgtatcttcc
     5941 ggagtgagag aagcaacagc ggggaagtta tcgtgacctt agagccctgt gagcgctcag
     6001 aaacctacgt aaatggcaag agggtgtccc agcctgttca gctgcgctca ggaaaccgta
     6061 tcatcatggg taaaaaccat gttttccgct ttaaccaccc ggaacaagca cgagctgagc
     6121 gagagaagac tccttctgct gagaccccct ctgagcctgt ggactggaca tttgcccaga
     6181 gggagcttct ggaaaaacaa ggaattgata tgaaacaaga gatggagaaa aggctacagg
     6241 aaatggagat cttatacaaa aaggagaagg aagaagcaga tcttcttttg gagcagcaga
     6301 gactggacta tgagagtaaa ttgcaggcct tgcagaagca ggttgaaacc cgatctctgg
     6361 ctgcagaaac aactgaagag gaggaagaag aggaagaagt tccttggaca cagcatgaat
     6421 ttgagttggc ccaatgggcc ttccggaaat ggaagtctca tcagtttact tcattacggg
     6481 acttactctg gggcaatgcc gtgtacctaa aggaggccaa tgccatcagt gtggaactga
     6541 aaaagaaggt gcagtttcag tttgttctgc tgactgacac actgtactcc cctttgcctc
     6601 ctgaattact tcccactgag atggaaaaaa ctcatgagga caggcctttc cctcgcacag
     6661 tggtagcagt agaagtccag gatttgaaga atggagcaac acactattgg tctttggaga
     6721 aactcaagca gaggctggat ttgatgcgag agatgtatga tagggcaggg gagatggcct
     6781 ccagtgccca agacgaaagc gaaaccactg tgactggcag cgatcccttc tatgatcggt
     6841 tccactggtt caaacttgtg gggagctccc ccattttcca cggctgtgtg aacgagcgcc
     6901 ttgccgaccg cacaccctcc cccacttttt ccacggccga ttccgacatc actgagctgg
     6961 ctgacgagca gcaagatgag atggaggatt ttgatgatga ggcattcgtg gatgacgccg
     7021 gctctgacgc agggacggag gagggatcag atctcttcag tgacgggcat gacccgtttt
     7081 acgaccgatc cccttggttc attttagtgg gaagggcatt tgtttacctg agcaatctgc
     7141 tgtatcccgt gcccctgatc cacagggtgg ccatcgtcag tgagaaaggt gaagtgcggg
     7201 gatttctgcg tgtggctgta caggccatcg cagcggatga agaagctcct gattatggct
     7261 ctggaattcg acagtcagga acagctaaaa tatcttttga taatgaatac tttaatcaga
     7321 gtgacttttc gtctgttgca atgactcgtt ctggtctgtc cttggaggag ttgaggattg
     7381 tggaaggaca gggtcagagt tctgaggtca tcactcctcc agaagaaatc agtcgaatta
     7441 atgacttgga tttgaagtca agcactttgc tggatggtaa gatggtaatg gaagggtttt
     7501 ctgaagagat tggcaaccac ctgaaactgg gcagtgcctt cactttccga gtaacagtgt
     7561 tgcaggccag tggaatcctc ccagagtatg cagatatctt ctgtcagttc aactttttgc
     7621 atcgccatga tgaagcattc tccacggagc ccctcaaaaa caatggcaga ggaagtcccc
     7681 tggcctttta tcatgtgcag aatattgcag tggagatcac tgaatcattt gtggattaca
     7741 tcaaaaccaa gcctattgta tttgaagtct ttgggcatta tcagcagcac ccacttcatc
     7801 tgcaaggaca ggagcttaac agtccgcctc agccgtgccg ccgattcttc cctccaccca
     7861 tgccactgtc caagccagtt ccagccacca agttaaacac gatgagcaaa accagccttg
     7921 gccagagcat gagcaagtat gacctcctgg tttggtttga gatcagtgaa ctggagccta
     7981 caggagagta tatcccagct gtggttgacc acacagcagg cttgccttgc caggggacat
     8041 ttttgcttca tcagggcatc cagcgaagga tcacagtgac cattatccat gagaagggga
     8101 gcgagctcca ttggaaagat gttcgtgaac tggtggtagg tcgtattcgg aataagcctg
     8161 aggtggatga agctgcagtt gatgccatcc tctccctaaa tattatttct gccaagtacc
     8221 tgaagtcttc ccacaactct agcaggacct tctaccgctt tgaggctgtg tgggatagct
     8281 ctctgcataa ctcccttctt ctgaaccgag tgacacccta tggagaaaag atctacatga
     8341 ccttgtcggc ctacctagag ctggatcatt gcatccagcc ggctgtcatc accaaggatg
     8401 tgtgcatggt cttctactcc cgagatgcca agatctcacc accacgctct ctgcgtagcc
     8461 tctttggcag cggctactca aagtcaccag attcgaatcg agtcactggc atttacgaac
     8521 tcagcttatg caaaatgtca gacacaggta gtccaggtat gcagagaagg agaagaaaaa
     8581 tcttagatac gtcagtggca tatgtgcggg gagaagagaa cttagcaggc tggcggcccc
     8641 gtggagacag cctcatcctt gagcaccagt gggagctgga gaagctggag ctcctacatg
     8701 aggtggaaaa aacccgccac tttttgctgc tgcgtgagag acttggtgac agcatcccca
     8761 aatccctgag cgactcgtta tcccccagcc tcagcagtgg gaccctcagc acctccacca
     8821 gtatctcctc tcagatctca accactacct ttgaaagcgc catcacacct agcgagagca
     8881 gtggctatga ttcaggagac atcgaaagcc tggtggaccg agagaaagag ctggctacca
     8941 agtgcctgca acttctcacc cacactttca acagagaatt cagccaggtg cacggcagcg
     9001 tcagtgactg taagttgtct gatatctctc caattggacg ggatccctct gagtccagtt
     9061 tcagcagtgc caccctcact ccctcctcca cctgtccctc tctggtagac tctaggagca
     9121 actctctgga tcagaagacc ccagaagcca attcccgggc ctctagtccc tgcccagaat
     9181 ttgaacagtt tcagattgtc ccagctgtgg aaacaccata tttggcccga gcaggaaaaa
     9241 acgaatttct caatcttgtt ccagatattg aagaaattag accaagctca gtggtctcta
     9301 agaaaggata ccttcatttc aaggagcctc tttacagtaa ctgggctaaa cattttgttg
     9361 tcgtccgtcg gccttatgtc ttcatctata acagtgacaa agaccctgtg gagcgtggaa
     9421 tcattaacct gtccacagca caggtggagt acagtgagga ccagcaggcc atggtgaaga
     9481 caccaaacac ctttgctgtc tgcacaaagc accgtggggt ccttttgcag gccctcaatg
     9541 acaaagacat gaacgactgg ttgtatgcct tcaacccact tctagctggc acaatacggt
     9601 caaagctttc ccgcagatgc ccgagccagt cgaaatactg agtcgacggt accatggtat
     9661 tgatatctga gctccgcatc ggccgctgtc atcagatcgc catctcgcgc ccgtgcctct
     9721 gacttctaag tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac
     9781 cccctatttt tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt
     9841 aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa
     9901 gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca
     9961 atgttctgtg tacacttctt atgtttttta cttctgataa atttttttga aacatcatag
    10021 aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt
    10081 tatttcttcg cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt
    10141 tggagtattt ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt
    10201 tgctttttgg ggtttcccct attgtttgtc aagatttcga ggacggcgtt tttcttgcta
    10261 aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg
    10321 ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct atggggtttt
    10381 tgccttaaat gacagaatac attcccaata taccaaacat aactgtttct actagtcggc
    10441 cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    10501 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    10561 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
    10621 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    10681 aataccgcat caggcggcct taattaaggg cctcgtgata cgcctatttt tataggttaa
    10741 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
    10801 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    10861 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    10921 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    10981 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    11041 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    11101 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
    11161 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    11221 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    11281 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    11341 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    11401 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    11461 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    11521 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    11581 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    11641 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    11701 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    11761 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    11821 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    11881 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    11941 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    12001 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    12061 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    12121 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    12181 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    12241 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    12301 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    12361 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    12421 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    12481 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    12541 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    12601 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccg
//
