LOCUS       sequence-440852-       10953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the gRNA targeting the FOXA-binding site in the human ZEB2 
            locus for the CRISPRd experiment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10953)
  AUTHORS   Matsui S, Granitto M, Buckley M, Ludwig K, Koigi S, Shiley J, 
            Zacharias WJ, Mayhew CN, Lim HW, Iwafuchi M
  TITLE     Pioneer and PRDM transcription factors coordinate bivalent 
            epigenetic states to safeguard cell fate.
  JOURNAL   Mol Cell. 2024 Jan 4:S1097-2765(23)01026-2. doi: 
            10.1016/j.molcel.2023.12.007.
  PUBMED    38211589
REFERENCE   2  (bases 1 to 10953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Jan 4:S1097-2765(23)01026-2. doi: 
            10.1016/j.molcel.2023.12.007."
COMMENT     Sequence Label: FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd
FEATURES             Location/Qualifiers
     source          1..10953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    88..213
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             260..440
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(458..656)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(483..503)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1209..1228
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1303..1407
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1408..1476,1477..2268)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1626..1645)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2439..3027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2928..2947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3181..3198
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3315..3336
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3351..3356,3357..3374,3375..3381)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3389..3405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3394..3416
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3413..3429
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3413..3429
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3478..3599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3489..3508)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3543..3562
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3729..4103)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(4122..4169)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(4217..4546)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4231..4366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4285..4304)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     4531..4551
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4560..4988)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4670..4691)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4882..4901
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5034..5258
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5247..5264
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             5290..5470
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5980..6568
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6086..6097
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6495..6515
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(6608..7321,7322..7324,7325..7327))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6611..7321,7322..7324,7325..7327))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6653..6674)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7002..7021
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7261..7282
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(7328..7381)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(7391..8011)
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     promoter        complement(8049..9260)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(8193..8212)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_RNA        complement(9296..9371)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    9398..9416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(9461..9480)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_feature    9671..9788
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(10086..10130)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10315..10548
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
       61 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
      121 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
      181 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
      241 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
      301 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
      361 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
      421 tggtctaacc agagagaccc agtacaggca aaacgcgctg cttatataga cctcccaccg
      481 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
      541 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
      601 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
      661 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
      721 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
      781 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
      841 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
      901 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
      961 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     1021 agtcaataat caatgtcaac gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg
     1081 cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta
     1141 aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca gatactggct
     1201 taactatgcg gcatcagagc agattgtact gagagtgcac cataggggat cgggagatct
     1261 cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1321 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1381 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1441 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1501 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1561 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1621 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     1681 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1741 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1801 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1861 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1921 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     1981 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2041 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2101 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     2161 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2221 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2281 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2341 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2401 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2461 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2521 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2581 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2641 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2701 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2761 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2821 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2881 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2941 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3001 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3061 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3121 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3181 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3241 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3301 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     3361 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     3421 agctatgacc atgattacgc caagctctag ctagaggtcg acggtataca gacatgataa
     3481 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     3541 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttg
     3601 gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc
     3661 cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt
     3721 agcacgtgtc agtcctgctc ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc
     3781 agggcgaact cccgccccca cggctgctcg ccgatctcgg tcatggccgg cccggaggcg
     3841 tcccggaagt tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc
     3901 acccacaccc aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac
     3961 agggtcacgt cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac
     4021 ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga
     4081 acggcactgg tcaacttggc catggtttag ttcctcacct tgtcgtatta tactatgccg
     4141 atatactatg ccgatgatta attgtcaaca cgtgctgatc agatccgaaa atggatatac
     4201 aagctcccgg gagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     4261 ggaatagctc agaggcagag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     4321 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     4381 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     4441 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     4501 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagaatta
     4561 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4621 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4681 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4741 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4801 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4861 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4921 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4981 gggcgcgtgg ggataccccc tagagcccca gctggttctt tccgcctcag aagccataga
     5041 gcccaccgca tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg
     5101 ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc
     5161 attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga
     5221 ggggcaaaca acagatggct ggcaactaga aggcacagtc gaggctgatc agcgggttta
     5281 aacgggccct gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     5341 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     5401 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     5461 agagagaccc agtacagtcc ggatgcagct ctcgggccat gtgatgaaat gctaggcggc
     5521 tgtcaaacct ccactctaat acttctctct ccgggtcatc catcccatgc aggctcacag
     5581 ggtgtaacaa gcgggtgttc tctccttcat tggcttcttc taccttctct tgctcaactg
     5641 gtactagctt gtagcaccat ccaaaggtca gtggatatct gatccctggc cctggtgtgt
     5701 agttctgcca atcagggaag tagccttgtg tgtggtagat ccacagatca aggatatctt
     5761 gtcttcgttg ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa
     5821 gatctacagc tgccttgtaa gtcattggtc ttaaaggtac ctgaggtgtg actggaaaac
     5881 ccacctcctc ctcctcttgt gcttctagcc aggcacaatc agcattggta gctgctgtat
     5941 tgctacttgt gattgctcca tgtttttcta ggtctcgatg cggggaggcg gcccaaaggg
     6001 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     6061 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     6121 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     6181 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     6241 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     6301 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     6361 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     6421 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     6481 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     6541 cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtagatcgc
     6601 ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     6661 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
     6721 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     6781 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     6841 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     6901 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     6961 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     7021 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     7081 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     7141 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     7201 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     7261 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     7321 tcaccattgg gccaggattc tcctcgacgt caccgcatgt tagcagactt cctctgccct
     7381 caccggaccc agagccagac tcacatttca gctgcttttc caggccacag atgatcagtt
     7441 ccaggccaaa aaggaatgca ggctcagccc cttggtggtc aaacaactca atagcttgcc
     7501 gcagcagtgg tggcatggag tcagtggtgg gggtctccct ctcctccttg gcaacttggt
     7561 gctcctggtc ctccagcaca cagcccaagg tgaagtggcc cacagcagag agggcataca
     7621 aggcattttc cagggagaag ccttgttggc acaggaatgc cagctggttc tccagggtct
     7681 catactgctt ctctgtaggc ctggtgccca agtgaacttt ggcaccatcc cggtgggaga
     7741 gcaaggcaca tctgaagctc ttggcattgt tcctcaggaa gtcctgccag gactcccctt
     7801 ccagagggca gaagtgggtg tggtgcctgt ccaacatctc aatggccagg gcatcaagca
     7861 gagccctctt gttcttcaca tgccagtaca gggtaggctg ctccacaccc agcttctggg
     7921 ccagcttcct ggtggtcaag ccctcaatgc caacttcatt caggagctcc aaggcagagt
     7981 tgatgacttt gctcttgtcc agtctggaca tggtggatcc tctagagtcg acctgcagcc
     8041 caagcttcgt ctaacaaaaa agccaaaaac ggccagaatt tagcggacaa tttactagtc
     8101 taacactgaa aattacatat tgacccaaat gattacattt caaaaggtgc ctaaaaaact
     8161 tcacaaaaca cactcgccaa ccccgagcgc atagttcaaa accggagctt cagctactta
     8221 agaagatagg tacataaaac cgaccaaaga aactgacgcc tcacttatcc ctcccctcac
     8281 cagaggtccg gcgcctgtcg attcaggaga gcctacccta ggcccgaacc ctgcgtcctg
     8341 cgacggagaa aagcctaccg cacacctacc ggcaggtggc cccaccctgc attataagcc
     8401 aacagaacgg gtgacgtcac gacacgacga gggcgcgcgc tcccaaaggt acgggtgcac
     8461 tgcccaacgg caccgccata actgccgccc ccgcaacaga cgacaaaccg agttctccag
     8521 tcagtgacaa acttcacgtc agggtcccca gatggtgccc cagcccatct cacccgaata
     8581 agagctttcc cgcattagcg aaggcctcaa gaccttgggt tcttgccgcc caccatgccc
     8641 cccaccttgt ttcaacgacc tcacagcccg cctcacaagc gtcttccatt caagactcgg
     8701 gaacagccgc cattttgctg cgctcccccc aacccccagt tcagggcaac cttgctcgcg
     8761 gacccagact acagcccttg gcggtctctc cacacgcttc cgtcccaccg agcggcccgg
     8821 cggccacgaa agccccggcc agcccagcag cccgctactc accaagtgac gatcacagcg
     8881 atccacaaac aagaaccgcg acccaaatcc cggctgcgac ggaactagct gtgccacacc
     8941 cggcgcgtcc ttatataatc atcggcgttc accgccccac ggagatccct ccgcagaatc
     9001 gccgagaagg gactactttt cctcgcctgt tccgctctct ggaaagaaaa ccagtgccct
     9061 agagtcaccc aagtcccgtc ctaaaatgtc cttctgctga tactggggtt ctaaggccga
     9121 gtcttatgag cagcgggccg ctgtcctgag cgtccgggcg gaaggatcag gacgctcgct
     9181 gcgcccttcg tctgacgtgg cagcgctcgc cgtgaggagg ggggcgcccg cgggaggcgc
     9241 caaaacccgg cgcggaggcc agatctggag ccgacacggg ttaattaaaa aaaaagcacc
     9301 gactcggtgc cactttttca agttgataac ggactagcct tattttaact tgctatttct
     9361 agctctaaaa cctcagggga tgtttactgg gcgggaatct ctatcactga tagggactta
     9421 taagattccc aaatccaaag acatttcacg tttatggtga tttcccagaa cacatagcga
     9481 catgcaaata ttgcagggcg ccactcccct gtccctcaca gccatcttcc tgccagggcg
     9541 cacgcgcgct gggtgttccc gcctagtgac actgggcccg cgattccttg gagcgggttg
     9601 atgacgtcag cgttcgaatt ccatggttaa ttaaccaaac tggatctctg ctgtccctgt
     9661 aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt
     9721 ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc
     9781 ttttaaaatt gtggatgaat actgccattt gtctgcagaa ttggcgcacg cagtgccgat
     9841 ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct
     9901 tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
     9961 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
    10021 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
    10081 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
    10141 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
    10201 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
    10261 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
    10321 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
    10381 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
    10441 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg
    10501 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
    10561 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
    10621 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
    10681 ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc
    10741 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata
    10801 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg
    10861 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac
    10921 tgcgaatcgt tctagctccc tgcttgccca tac
//
