LOCUS       sequence-440857-        9407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 
            safe harbour locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Batty P, Langer CC, Takacs Z, Tang W, Blaukopf C, Peters JM, Gerlich
            DW
  TITLE     Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2
            phase.
  JOURNAL   EMBO J. 2023 Aug 15;42(16):e113475. doi: 10.15252/embj.2023113475. 
            Epub 2023 Jun 26.
  PUBMED    37357575
REFERENCE   2  (bases 1 to 9407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-08-15"; volume: "42(16):e113475. doi"; pages: " 
            10.15252/embj.2023113475. Epub 2023 Jun 26"
COMMENT     Sequence Label: RepairTemplate_AAVS1-OsTIR1-F74G-SNAP
FEATURES             Location/Qualifiers
     source          1..9407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(54..73)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      867..1455
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1356..1375
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1609..1626
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2171..3007
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     polyA_signal    3035..3116
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3060..3079
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3144..3542)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3555..4126
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3715..3734)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     3944..3961
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(4182..4201)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          4184..4413
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     CDS             complement(4506..5048)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             complement(5058..5087)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(5094..5123)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(5130..5159)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(5187..6911)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein, 
                     mutated for use in the AID2 system of inducible degradation
                     (Yesbolatova et al., 2020)"
                     /label=OsTIR1(F74G)
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHGADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     promoter        complement(6936..7147)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    7157..7248
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(7189..7208)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    7262..7310
                     /note="synthetic polyadenylation signal"
     misc_feature    7336..8139
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     rep_origin      complement(8656..9111)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8793..8814)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9005..9024
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9138..9242
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9243..9311,9312..9407,1..696)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
       61 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      121 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      181 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      241 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      301 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      361 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      421 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      481 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      541 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      601 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      661 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
      721 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
      781 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      841 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      901 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      961 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1021 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1081 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1141 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1201 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1261 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1321 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1381 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1441 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1501 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1561 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     1621 agcgaggaag cggaagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
     1681 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
     1741 gaaggctctc aagggcatcg gtcgagccca gagacagtga ccaaccatcc ctgttttcct
     1801 aggactgagg gtttcagcgc taaaactagg ctgtcctggg caaacagcat aagctggtca
     1861 ccccacaccc agacctgacc caaacccagc tcccctgctt cttggccacg taacctgaga
     1921 agggaatccc tcctctctga accccagccc accccaatgc tccaggcctc ctgggatacc
     1981 ccgaagagtg agtttgccaa gcagtcaccc cacagttgga ggagaatcca cccaaaaggc
     2041 agcctggtag acagggctgg ggtggcctct cgtggggtcc aggccaagta ggtggcctgg
     2101 ggcctctggg ggatgcaggg gaagggggat gcaggggaac ggggatgcag gggaacgggg
     2161 ctcagtctga agagcagagc caggaacccc tgtagggaag gggcaggaga gccaggggca
     2221 tgagatggtg gacgaggaag ggggacaggg aagcctgagc gcctctcctg ggcttgccaa
     2281 ggactcaaac ccagaagccc agagcagggc cttagggaag cgggaccctg ctctgggcgg
     2341 aggaatatgt cccagatagc actggggact ctttaaggaa agaaggatgg agaaagagaa
     2401 agggagtaga ggcggccacg acctggtgaa cacctaggac gcaccattct cacaaaggga
     2461 gttttccaca cggacacccc cctcctcacc acagccctgc caggacgggg ctggctactg
     2521 gccttatctc acaggtaaaa ctgacgcacg gaggaacaat ataaattggg gactagaaag
     2581 gtgaagagcc aaagttagaa ctcaggacca acttattctg attttgtttt tccaaactgc
     2641 ttctcctctt gggaagtgta aggaagctgc agcaccagga tcagtgaaac gcaccagaca
     2701 gccgcgtcag agcagctcag gttctgggag agggtagcgc agggtggcca ctgagaaccg
     2761 ggcaggtcac gcatcccccc cttccctccc accccctgcc aagctctccc tcccaggatc
     2821 ctctctggct ccatcgtaag caaaccttag aggttctggc aaggagagag atggctccag
     2881 gaaatggggg tgtgtcacca gataaggaat ctgcctaaca ggaggtgggg gttagaccca
     2941 atatcaggag actaggaagg aggaggccta aggatggggc ttttctgtca ccaatcctgt
     3001 ccctagtgat gcatggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     3061 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     3121 ccgataagct tgatatcgaa ttcttagccc tcccacacat aaccagaggg cagcaattca
     3181 cgaatcccaa ctgccgtcgg ctgtccatca ctgtccttca ctatggcttt gatcccagga
     3241 tgcagatcga gaagcacctg tcggcaccgt ccgcaggggc tcaagatgcc cctgttctca
     3301 tttccgatcg cgacgataca agtcaggttg ccagctgccg cagcagcagc agtgcccagc
     3361 accacgagtt ctgcacaagg tcccccagta aaatgatata cattgacacc agtgaagatg
     3421 cggccgtcgc tagagagagc tgcgctggcg acgctgtagt cttcagagat ggggatgctg
     3481 ttgattgtag ccgttgctct ttcaatgagg gtggattctt cttgagacaa aggcttggcc
     3541 atggaaggtc gtctttatca tcgtgttttt caaaggaaaa ccacgtcccc gtggttcggg
     3601 gggcctagac gtttttttaa cctcgactaa acacatgtaa agcatgtgca ccgaggcccc
     3661 agatcagatc ccatacaatg gggtaccttc tgggcatcct tcagcccctt gttgaatacg
     3721 cttgaggaga gccatttgac tctttccaca actatccaac tcacaacgtg gcactggggt
     3781 tgtgccgcct ttgcaggtgt atcttataca cgtggctttt ggccgcagag gcacctgtcg
     3841 ccaggtgggg ggttccgctg cctgcaaagg gtcgctacag acgttgtttg tcttcaagaa
     3901 gcttccagag gaactgcttc cttcacgaca ttcaacagac cttgcattcc tttggcgaga
     3961 ggggaaagac ccctaggaat gctcgtcaag aagacagggc caggtttccg ggccctcaca
     4021 ttgccaaaag acggcaatat ggtggaaaat aacatataga caaacgcaca ccggccttat
     4081 tccaagcggc ttcggccagt aacgttaggg ggggggggga gaggggcgga attggccgcc
     4141 ctagatgcat gctcgacctg cagttggacc tgggagtgga cacctgtgga gagaaaggca
     4201 aagtggatgt cattgtcact caagtgtatg gccagatctc aagcctgcca cacctcaagc
     4261 ttgacaacaa aaagattgtc ttttctgacc agatggacgc ggccaccctc aaaggcatca
     4321 ccgcgggcca ggtgaatatc aaatcctcct cgtttttgga aactgacaat cttagcgcag
     4381 aagtcatgcc cgcttttgag agggagtact caccccaaca gctggccctc gcagacagcg
     4441 aattaattcg gcggccggtt aacctaatca agtttgaccc gtttggctgc ggctctagaa
     4501 agcttaccca gcccaggctt gcccagtctg tggccctcgt gggccagcag ccactctttc
     4561 acggcgagcc cgccctcgta gccccccacg tccaggtcgc cctgcaccac ccggtggcag
     4621 gggatcagaa tgggcacggg atttccgctc agggcggttt tcacggcggc ggtggcggcg
     4681 ggattgccgg ccagggcggc caggtggctg tagctgatga cctctccgaa cttcaccact
     4741 ttcagcagtt tccacagcac ctggcgggta aagctctcct gctggaacac tgggtggtgc
     4801 agggctggca cagggaactc ctcgatggcc tcaggctggt gaaagtaggc gttgagccag
     4861 gcggtggcct gcatcagtgg ctctggtccg cccagcacgg cggctggggc aggcacttcc
     4921 acggcgtcgg cggcagatgt tcctttgccc aggaagatga tacggtgcag gccctgttcg
     4981 cacccagaca gttccagctt gcccagaggg ctatccaggg tggtgcgctt catttcgcag
     5041 tctttgtcgg atccgttcaa gtcttcttct gagattaatt tttgttcacc gttcaagtct
     5101 tcctcggaga ttagcttttg ttcaccgttc aaatcttctt cagaaatcaa cttttgttca
     5161 ccactagcag cagaaccgga gtcgactagg attttaacaa aatttggtgc atcatccctc
     5221 gccccagcag ttgtgcggta cacatataat ttctccactt taggcagatc tccatggttt
     5281 tcctccattt cattgctacc atcccgctca tttatgacct caacattgag catcggcatc
     5341 tttgacgcaa ggacttggca cccctttaac gtgacattgc aagatgacat ccaaagggat
     5401 cgcattgtct cgtacctagc aaaattcccc aagagtgcag catcaccaaa cgggctatct
     5461 cttatctcca gtttccttaa attcttgcat ccattcataa catgcatcat acccttatca
     5521 ctgtcaccag caaatgctat agaaagcatc tcaagttgtt ttgcatattt cccaatatac
     5581 atgaaaactt tgtctgtgag aagaccagat attgacaaac gttgtaatcc cttgcactca
     5641 cgaacaatag ctccaaagcc ttcatctaat ggttggcttg tcacaacatc aggcttccct
     5701 ggctcaagaa tacaaagtct gaatcgtgtg aaatttggac agttcttggc gacagtaact
     5761 agtgcagcat tagtcatttg gtgacagaag tacagtagtg agttcagttt tggacagccc
     5821 aaggatactg caacaagtcc ctcctctgtc actgcagaat aaccagctac gtagaaatct
     5881 gatggaaata ccctgagttc ttgcaagtct ttgcaactgg aggccaccac ttgcaagcct
     5941 ttgtccgaga tacaatccag tacccaaagg cgttggagct tcacacagcg gctaatcatt
     6001 tttgtaaggt cagaagcatc aagtgtgggt gcatagctca agttcaatcc tgtcagttga
     6061 gcacacaggg ggtagataaa tgacaggcaa acaggagaag catcccagaa tccagacaaa
     6121 ctcctcaaca tcttgcattt ctccagagca ctggtaagct taaagtagga ctcagtttgg
     6181 aagtcatctg tcaaattccc tgtccccaaa tcctccaagt taggggtacg cagtagtatc
     6241 tttgcaagtg tatctaccga tacagatcga ttcagcctca aactccgcag gtttggggac
     6301 ctgctaacaa gtctctccag tgaaccagca ttaacctccc ctttgatgca ggcaaaattc
     6361 aatgagacaa gtgatgtgca ggaatcaggg aagcaggaaa gccacctagg ccctcgatcc
     6421 tccacttcat tttcctgcaa atccaactcc ctcagaagct tgcaatggct cgcgacggcg
     6481 gctagcccgt cagtgctgaa cccctcgcag ctgataagaa caagagccct gaaccgcggg
     6541 aacgagcgag ccagcagttc gaggctctcg tcggacacca ccatccgctt catcctgagc
     6601 tcctccaggc cgtggcatcc cctcgcggcc gcctcgatcc acggccccgc gtagccgccc
     6661 cagtcggggg gcacgaggtt gaagtcggcg ccgtggggct tccccttcac cgtgagcgcc
     6721 cgcacgttgg ggaaccgcgc ggcgacgcgg ccggcgcgca cggcgtagca gttgcccacg
     6781 aagacgccgc ggcggctcag cctctcgatc tcgtaccaca ccttgcagac gagcgagacc
     6841 gtgttgcggt cgcgctgcgc cggcaggaag ctgaagatgt gctccaccac ctcctccggg
     6901 aagtacgtca tggtggaagc gctctagaac cggtcctgtg ttctggcggc aaacccgttg
     6961 cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc accctcggga
     7021 aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca ccttctctag
     7081 gcaccggatc aattgccgac ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc
     7141 tctgcccact actagtagag aaatgttctg gcacctgcac ttgcactggg gacagcctat
     7201 tttgctagtt tgttttgttt cgttttgttt tgatggagag cgtatgttag tactatcgat
     7261 tcacacaaaa aaccaacaca cagatgtaat gaaaataaag atattttatt ctcgaggtga
     7321 taattccacg catgcgcccc actgtggggt ggaggggaca gataaaagta cccagaacca
     7381 gagccacatt aaccggccct gggaatataa ggtggtccca gctcggggac acaggatccc
     7441 tggaggcagc aaacatgctg tcctgaagtg gacatagggg cccgggttgg aggaagaaga
     7501 ctagctgagc tctcggaccc ctggaagatg ccatgacagg gggctggaag agctagcaca
     7561 gactagagag gtaagggggg taggggagct gcccaaatga aaggagtgag aggtgacccg
     7621 aatccacagg agaacggggt gtccaggcaa agaaagcaag aggatggaga ggtggctaaa
     7681 gccagggaga cggggtactt tggggttgtc cagaaaaacg gtgatgatgc aggcctacaa
     7741 gaaggggagg cgggacgcaa gggagacatc cgtcggagaa ggccatccta agaaacgaga
     7801 gatggcacag gccccagaag gagaaggaaa agggaaccca gcgagtgaag acggcatggg
     7861 gttgggtgag ggaggagaga tgcccggaga ggacccagac acggggagga tccgctcaga
     7921 ggacatcacg tggtgcagcg ccgagaagga agtgctccgg aaagagcatc cttgggcagc
     7981 aacacagcag agagcaaggg gaagagggag tggaggaaga cggaacctga aggaggcggc
     8041 agggaaggat ctgggccagc cgtagaggtg acccaggcca caagctgcag acagaaagcg
     8101 gcacaggccc aggggagaga atgcaggtca gagaaagcag gacctgcctg ggaaggggaa
     8161 acagtgggcc agaggcggcg cagaagccag tagagctcaa agtggtccgg actcaggaga
     8221 gagacggcag cgttagaggg cagagttccg gcggcacagc aagggcactc gggggcgaga
     8281 ggagggcagc gcaaagtgac aatggccagg gccaggcaga tagaccagac tgagctatgg
     8341 gagctggctc aggttcagga gagggcaggg cagggaagga gacaaagtcc aggaccggct
     8401 ggaggggctc aacatcggaa gaggggaagt cgagggaggg atggtaagga ggactgcatg
     8461 ggtcagcaca ggctgccaaa gccagggcca gttaaagcga ctccaatgcg gaagagagta
     8521 ggtcgacggc cgctccaagt acctcccgta ccttaatatt acttacttat catggtagct
     8581 tgggctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     8641 tgaatggcga atggcaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     8701 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     8761 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     8821 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     8881 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     8941 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     9001 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     9061 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     9121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9181 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9241 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9301 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9361 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatcctt
//
