LOCUS       sequence-440859-       13786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EF1-alpha-driven expression of miniTurbo fused to dCasRx with GFP 
            (Adapted from plasmid #153209).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13786)
  TITLE     LncRNA CISTR-ACT regulates cell size in human and mouse
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: miniTurbo-dCasRx
FEATURES             Location/Qualifiers
     source          1..13786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    134..251
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(549..593)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    778..1011
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1504..1629
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1676..1856
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1874..2072)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1899..1919)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2074..2453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2625..2644
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2719..2823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2824..2892,2893..3684)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3855..4443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4344..4363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4597..4614
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4731..4752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4767..4772,4773..4790,4791..4797)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4805..4821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4810..4832
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4829..4845
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4829..4845
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4894..5015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4905..4924)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4959..4978
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5145..5519)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(5538..5585)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5633..5962)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5647..5782
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5701..5720)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5947..5967
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(5976..6404)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6086..6107)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6298..6317
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    6450..6674
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6663..6680
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             6706..6886
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     7391..7407
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7392..7408
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7411..7999
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7517..7528
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7926..7946
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(8024..8737,8738..8740))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(8027..8737,8738..8740))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8069..8090)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8418..8437
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(8741..8794)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(8810..8836)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(8846..8866)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8876..11773)
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             complement(11783..11803)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(11822..12592)
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     regulatory      12595..12604
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(12617..13786,1..9))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          12626..13564
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(12649..12669)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 accggagcca attcccactc ctttcaagac ctagaaggtc cattagctgc aaagattcct
       61 ctctgtttaa aactttatcc atctttgcat taattaacca aactggatct ctgctgtccc
      121 tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta
      181 tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca atcccccctt
      241 ttcttttaaa attgtggatg aatactgcca tttgtctgca gaattggcgc acgcagtgcc
      301 gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt
      361 ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa
      421 tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct taaacctacc
      481 aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac
      541 caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc
      601 tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg
      661 tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta
      721 gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg
      781 agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg
      841 ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc
      901 tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct
      961 gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc tattcccact
     1021 gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac tcctaatggt
     1081 tcaattttta ctactttata tttatataat tcacttctcc aattgtccct catatctcct
     1141 cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc
     1201 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
     1261 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
     1321 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt
     1381 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
     1441 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
     1501 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
     1561 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
     1621 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
     1681 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     1741 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     1801 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     1861 caggcaaaac gcgctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     1921 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     1981 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     2041 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     2101 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     2161 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     2221 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     2281 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     2341 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     2401 aactaatgac cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt
     2461 atatctggcc cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat
     2521 gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc
     2581 gacctccaac acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat
     2641 tgtactgaga gtgcaccata ggggatcggg agatctcccg atccgtcgac gtcaggtggc
     2701 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     2761 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     2821 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     2881 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     2941 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     3001 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     3061 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     3121 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     3181 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     3241 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     3301 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     3361 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     3421 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     3481 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     3541 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     3601 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     3661 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     3721 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     3781 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3841 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3901 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3961 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     4021 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4081 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4141 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4201 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     4261 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4321 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4381 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4441 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4501 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4561 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4621 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4681 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     4741 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     4801 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     4861 ctctagctag aggtcgacgg tatacagaca tgataagata cattgatgag tttggacaaa
     4921 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     4981 tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa ctccagcatg
     5041 agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac
     5101 ctttcataga aggcggcggt ggaatcgaaa tctcgtagca cgtgtcagtc ctgctcctcg
     5161 gccacgaagt gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg cccccacggc
     5221 tgctcgccga tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc
     5281 tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg
     5341 tccggcacca cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca
     5401 ccggcgaagt cgtcctccac gaagtcccgg gagaacccga gccggtcggt ccagaactcg
     5461 accgctccgg cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa cttggccatg
     5521 gtttagttcc tcaccttgtc gtattatact atgccgatat actatgccga tgattaattg
     5581 tcaacacgtg ctgatcagat ccgaaaatgg atatacaagc tcccgggagc tttttgcaaa
     5641 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gcagaggcgg
     5701 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     5761 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     5821 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     5881 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     5941 ttccacaccc taactgacac acattccaca gaattaattc gcgttaaatt tttgttaaat
     6001 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     6061 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     6121 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     6181 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     6241 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     6301 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     6361 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat accccctaga
     6421 gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc cagcatgcct
     6481 gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga
     6541 atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg
     6601 agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca
     6661 actagaaggc acagtcgagg ctgatcagcg ggtttaaacg ggccctgcta gagattttcc
     6721 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     6781 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     6841 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta cagtccggat
     6901 gcagctctcg ggccatgtga tgaaatgcta ggcggctgtc aaacctccac tctaatactt
     6961 ctctctccgg gtcatccatc ccatgcaggc tcacagggtg taacaagcgg gtgttctctc
     7021 cttcattggc ttcttctacc ttctcttgct caactggtac tagcttgtag caccatccaa
     7081 aggtcagtgg atatctgatc cctggccctg gtgtgtagtt ctgccaatca gggaagtagc
     7141 cttgtgtgtg gtagatccac agatcaagga tatcttgtct tcgttgggag tgaattagcc
     7201 cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca
     7261 ttggtcttaa aggtacctga ggtgtgactg gaaaacccac ctcctcctcc tcttgtgctt
     7321 ctagccaggc acaatcagca ttggtagctg ctgtattgct acttgtgatt gctccatgtt
     7381 tttctaggtc tcgaggtcga cggtatcgat gcggggaggc ggcccaaagg gagatccgac
     7441 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg
     7501 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
     7561 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca
     7621 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
     7681 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
     7741 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
     7801 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
     7861 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
     7921 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
     7981 tgtaatccag aggttgatta tcgataagct tgatatcgaa ttcttacttg tacagctcgt
     8041 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     8101 tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca
     8161 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     8221 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     8281 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     8341 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     8401 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     8461 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     8521 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     8581 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     8641 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     8701 tgggcaccac cccggtgaac agctcctcgc ccttgctcac tgggccagga ttctcctcga
     8761 cgtcaccgca tgttagcaga cttcctctgc cctctccact gccgctagca gcgtaatctg
     8821 gaacatcgta tgggtaagcg gccgccacct tcctcttttt cttaggtccg gatccggaat
     8881 tgccggacac cttctttttc tccttgtcga acttggcggc ctcgttccta tcgaacaggg
     8941 cctcgatgct caggttctta aacctgggga tacagtagcc gaaaggcaca cacagcagtt
     9001 tcaggagcct atcgttgtac ttcttctcgt cattcacagc gtcgaagtac tcggacacct
     9061 ttccgctgct tttctcgtac ctctcattca tgataattct ctgcatgatg taatggtaca
     9121 gttggaagta ggaattgacc tcggcaatgt cgttgatata ggcgtggaca tacctggcca
     9181 cttccagggc gacggccttg tttgcgaaca gggtacatgt cttgttgtca attctcagga
     9241 ggtcctccct gatgatcaca ttccacttgg tgttcctcag gtaggcgttc agggcggttt
     9301 tggccttctc cctgttaatc tgcctggtga actcctcggc tttcttctcg tcgctgtatt
     9361 tgatgtaatt ggcatacagc ttggggttgg cgctctcgag gctgtcaatg ctctccttgg
     9421 ctctttcagc catctccttt tccacgtcct ttctcttatc gggggcagtt tcatcaatgc
     9481 cagcgcagag cttggtgacg gagctgaatc ccttctcttc cagtttcttg agattgatgt
     9541 cgtagccttt ctccttgtac agttgagcat cacgctcgac gcaatggaat ccgatgacgt
     9601 acctggcgtt gatattgaca atattcttga ggatgtggta gatcacggtg aggtacaggc
     9661 tgatgatctt tttaaacttc tccctctcgg cgttttccct gccggtgtcc tcaatgacgc
     9721 tccttttctt gtcgaattgg tcgtagttca ttccggtgat gatctttgtg agagcgtcca
     9781 ccttttcgct cacgctcttg cccttatcct ttccgataca agtttcgtag tacctgtcga
     9841 tctggttctt gccgttctgg ccctgttttt tctggatgtc agcgatcctg ccgagcacga
     9901 acttcaccac ggcctcgttt ttggcgatct catggaggtg ggcaggatca ccgtatctga
     9961 tcaggtagtg gaacctttta ttgctgatca cgttattaat aatgaaattt ctcatgccgt
    10021 gcttgccttt cttgagcttg tttccgttct cgtccaggga aaaggtgtcg gcgagggcct
    10081 tgagctcatc ataggacagg ttggttccta aaatacggat ggcgtcgata tacatggccc
    10141 tcctggcatc ggcaataggt tctcccattc tagcgaagga cttgatcagc ctcagctcat
    10201 cggcgatctt ggcggagtct ttgaaaaagg cgtattcctc cacgaactta gcgttgactc
    10261 cgatgagagg catcaccttc aggaagctct ggatgttatc gaatttatta atcagggtgg
    10321 tcaggaggtc gttgatctcc ttgccatcca ggaacatggt cagggcatac atgagtttgc
    10381 tgaaggcgga aacatcacgg ccagcgggca ggattctggg cagtctaggg gcgtccttct
    10441 tcttatactc tcttgtcttg tttcccctaa attccttgat gttgtgcatg atattttcga
    10501 gctttctcca aattctatta gcttcatcgt agtagagggc atccttctgg tcgtcgttga
    10561 aggagcccct caggttaatc acaaagatat ccttctcgct cagggacttc tcattatcgg
    10621 ggagggactt attggcggca gccaccttgg catcctcttc gatgtaatac ctataaatca
    10681 caaagtccat catggtgtag accttggtcc tgatggagtc gaacacctta tgatttttcc
    10741 tgatctcgga catatccttc ctgtccagca tcacttccct gagcttggtg atattgaatc
    10801 cgaggttttt ctgctctttc ataatgctga atctgaaata ttgttcggcg aattcggcag
    10861 ggttgattcc cagagtttcg gcaatatagt tcacgttggc ggcggagttc ttggagaagg
    10921 agttggtcag ctcattggtg atcctgtcgt agaggtagtt gagggtggag atgtattcgt
    10981 tgtcgaggtt cttatcgagg ttgtagagcc aggtcctgga gatcctggac tcttcttcgt
    11041 tgttagcgac cacccagtgc gccagtccgc tcaggagggc cagaatgtca tagcattcgt
    11101 tgccgtaatt gatgatgtaa tttctgccct ccttgctgaa aaaggcctgg ccgaaatagc
    11161 cgagtctggg gttatcgagg aagttgtcga actcgtcata ctgggccttg atggcgttga
    11221 tgagcttatc gttattgttg aaagcggccc tatggtgctc ggggtctttg aattcgtcgt
    11281 aggtatacac tgtggagaac ttgccgaatc caataatgtc cttatccagg ccggagatat
    11341 tgttgacggc gtaggcggcg ttggtaatgt attcggcgag gattttttca atgtccagga
    11401 tgttatggat cacctggata caaatattgt cattgccatc agcgctctcg ccgaagtacc
    11461 tcttttccag agtttccttc aggccgagca tatcctgctg gacgggtcct gtatacagag
    11521 ggttgttagc caccacggcg tagcccttag gatggctgaa tttggcgttg ccgatcttat
    11581 agccggcgtt tttatcggcc atttcagcgc tgaaggcctc gccctcattc acgctcctga
    11641 tgctgtcgcc ctccacgatc ttttccagcc tggcgtcgct gccttcggcg aaggttgtca
    11701 tgtacacttt ggagccggac acgagtgtgg acttcacgcc catgcccttg gcgaaggact
    11761 tttttttttc gatgctggcc tccacctttc tcttcttctt ggggctcatg gtggccgtac
    11821 gcttttcggc agaccgcaga ctgatttctc cgcccatcca gggtttgata actccgtcct
    11881 gttccagcag cagggctccc tgtttatcaa tcccccggct aatcccgaaa atctctttgt
    11941 ccccaatgat cagcttcacg ggtctattga tgaagttatc cagcttctcc caccgtgaca
    12001 gatatggagc caggccttcc tgctcgaaca gttccagggc tgcccgcagc tctcggatca
    12061 gcatagcggc cagagtattc ctgtccaggt taatccctgc ttcctgcagt gtgatccagc
    12121 cctgattgac cacgctttcc tccacgcgcc tcatagccac gttaatcccg gcgccaatga
    12181 cgatctgtgc agcatcgcct gttattccgg ccagctccac caggatgcct gccagctttc
    12241 tatcctgcag atacaggtca ttgggccatt tgactcgcac cttgtctgct cccagctttc
    12301 gcagcgcttc tgccatgaca attccgatga ccgggcccag gccgattgct gctggtcccc
    12361 gcttcaggcg ccaaaacata ctcaggtaca ggttagctcc aaaaggagag aaccatttcc
    12421 tccctctgct tcctctccct gcctgctgat attctgcaat gcaagcatcc ccactcttca
    12481 gctcgccgat tcgatccagc aggtactgat tggtggagtc gaccacaggc aggactgcca
    12541 cgctcccgcc gtccagctgt cccagaatct gtttagcgtt cagcagcggg atcgccatgg
    12601 tggcggtggc cgtacgtcac gacacctgaa atggaagaaa aaaactttga accactgtct
    12661 gaggcttgag aatgaaccaa gatccaaact caaaaagggc aaattccaag gagaattaca
    12721 tcaagtgcca agctggccta acttcagtct ccacccactc agtgtgggga aactccatcg
    12781 cataaaaccc ctccccccaa cctaaagacg acgtactcca aaagctcgag aactaatcga
    12841 ggtgcctgga cggcgcccgg tactccgtgg agtcacatga agcgacggct gaggacggaa
    12901 aggccctttt cctttgtgtg ggtgactcac ccgcccgctc tcccgagcgc cgcgtcctcc
    12961 attttgagct ccctgcagca gggccgggaa gcggccatct ttccgctcac gcaactggtg
    13021 ccgaccgggc cagccttgcc gcccagggcg gggcgataca cggcggcgcg aggccaggca
    13081 ccagagcagg ccggccagct tgagactacc cccgtccgat tctcggtggc cgcgctcgca
    13141 ggccccgcct cgccgaacat gtgcgctggg acgcacgggc cccgtcgccg cccgcggccc
    13201 caaaaaccga aataccagtg tgcagatctt ggcccgcatt tacaagacta tcttgccaga
    13261 aaaaaagcgt cgcagcaggt catcaaaaat tttaaatggc tagagactta tcgaaagcag
    13321 cgagacaggc gcgaaggtgc caccagattc gcacgcggcg gccccagcgc ccaggccagg
    13381 cctcaactca agcacgaggc gaaggggctc cttaagcgca aggcctcgaa ctctcccacc
    13441 cacttccaac ccgaagctcg ggatcaagaa tcacgtactg cagccaggtg gaagtaattc
    13501 aaggcacgca agggccataa cccgtaaaga ggccaggccc gcgggaacca cacacggcac
    13561 ttacctgtgt tctggcggca aacccgttgc gaaaaagaac gttcacggcg actactgcac
    13621 ttatatacgg ttctccccca ccctcgggaa aaaggcggag ccagtacacg acatcacttt
    13681 cccagtttac cccgcgccac cttctctagg caccggttca attgccgacc cctcccccca
    13741 acttctcggg gactgtgggc gatgtgcgct ctgcccactg acgggc
//
