LOCUS       sequence-440866-        8517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes CRISPR sgRNA targeting human B3GNT5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8517)
  TITLE     Fucosylated glycoproteins and fucosylated glycolipids play opposing 
            roles in cholera intoxication
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: lentiGuideB3GNT5-neo
FEATURES             Location/Qualifiers
     source          1..8517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             137..370
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    448..569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(485..504)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     539..558
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      609..744
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(764..783)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(765..783)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(793..810)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(793..809)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(802..824)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      951..1406
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1038..1057)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1248..1269
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1432..1536
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1537..1605,1606..2397)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1755..1774)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2568..3156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3057..3076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3310..3327
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3444..3465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3480..3485,3486..3503,3504..3510)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3518..3534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3523..3545
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3542..3558
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3542..3558
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3577..3597
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3579..3597
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3625..3851
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3852..4032
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4079..4204
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4697..4930
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5115..5159
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5337..5585
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5337..5357
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5508..5527
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5606..5681
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5737..5854
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5993..7171
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6224..7162
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7119..7139
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7184..7978
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7238..7257)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7848..7867
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    join(7994..8517,1..65)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8047..8067)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8465..8476)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
       61 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
      121 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
      181 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      241 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
      301 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
      361 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
      421 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
      481 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
      541 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
      601 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      661 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
      721 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
      781 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      841 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      901 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
      961 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     1021 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1081 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1141 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     1201 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     1261 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     1321 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     1381 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     1441 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1501 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1561 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1621 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1681 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1741 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     1801 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     1861 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     1921 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     1981 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     2041 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     2101 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     2161 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2221 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     2281 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2341 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2401 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2461 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2521 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2581 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2641 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2701 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2761 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2821 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2881 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2941 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3001 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3061 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3121 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3181 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3241 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3301 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3361 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3421 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     3481 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     3541 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     3601 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     3661 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     3721 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     3781 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     3841 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3901 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3961 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4021 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     4081 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     4141 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     4201 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     4261 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     4321 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     4381 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     4441 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     4501 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     4561 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     4621 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     4681 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     4741 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     4801 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     4861 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     4921 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     4981 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     5041 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     5101 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     5161 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     5221 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     5281 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccagagagg
     5341 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
     5401 ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
     5461 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
     5521 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     5581 acaccgggat tggtcgtgtt catcggtttt agagctagaa atagcaagtt aaaataaggc
     5641 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttaag cttggcgtaa
     5701 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     5761 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     5821 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     5881 tccactttgg cgccggctcg agggggcccg ggtgcaaaga tggataaagt tttaaacaga
     5941 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
     6001 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     6061 cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     6121 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     6181 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     6241 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     6301 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     6361 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     6421 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     6481 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     6541 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     6601 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     6661 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     6721 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     6781 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     6841 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     6901 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     6961 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     7021 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     7081 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     7141 aagttttttt cttccatttc aggtgtcgtg acgtacggcc accatgattg aacaagatgg
     7201 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     7261 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     7321 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     7381 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     7441 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     7501 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     7561 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     7621 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     7681 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     7741 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     7801 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     7861 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     7921 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaac
     7981 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     8041 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     8101 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     8161 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     8221 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     8281 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     8341 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     8401 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     8461 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcg
//
