LOCUS       sequence-440867-        8517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes CRISPR sgRNA targeting human SLC3C1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8517)
  TITLE     Fucosylated glycoproteins and fucosylated glycolipids play opposing 
            roles in cholera intoxication
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: lentiGuideSLC35C1-neo
FEATURES             Location/Qualifiers
     source          1..8517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          6..944
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(29..49)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1314..1431
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(1487..1562)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(1583..1831)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(1641..1660)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(1811..1831)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(2009..2053)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2238..2471
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2964..3089
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3136..3316
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3317..3543)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(3571..3591)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3571..3589)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3610..3626)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3610..3626)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3623..3645)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3634..3650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3658..3664,3665..3682,3683..3688))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3703..3724
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3841..3858)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4012..4600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4092..4111)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4771..5562,5563..5631))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5394..5413
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5632..5736)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5762..6217)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5899..5920)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6111..6130
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6344..6366
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6358..6375
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6359..6375
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6385..6404
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6385..6403
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6424..6559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6599..6720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6610..6629)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6664..6683
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             6798..7031
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7103..7691
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7209..7220
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7618..7638
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7707..8501)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7818..7837)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8428..8447
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(8514..8517,1..1175))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gacacctgaa atggaagaaa aaaactttga accactgtct gaggcttgag aatgaaccaa
       61 gatccaaact caaaaagggc aaattccaag gagaattaca tcaagtgcca agctggccta
      121 acttcagtct ccacccactc agtgtgggga aactccatcg cataaaaccc ctccccccaa
      181 cctaaagacg acgtactcca aaagctcgag aactaatcga ggtgcctgga cggcgcccgg
      241 tactccgtgg agtcacatga agcgacggct gaggacggaa aggccctttt cctttgtgtg
      301 ggtgactcac ccgcccgctc tcccgagcgc cgcgtcctcc attttgagct ccctgcagca
      361 gggccgggaa gcggccatct ttccgctcac gcaactggtg ccgaccgggc cagccttgcc
      421 gcccagggcg gggcgataca cggcggcgcg aggccaggca ccagagcagg ccggccagct
      481 tgagactacc cccgtccgat tctcggtggc cgcgctcgca ggccccgcct cgccgaacat
      541 gtgcgctggg acgcacgggc cccgtcgccg cccgcggccc caaaaaccga aataccagtg
      601 tgcagatctt ggcccgcatt tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt
      661 catcaaaaat tttaaatggc tagagactta tcgaaagcag cgagacaggc gcgaaggtgc
      721 caccagattc gcacgcggcg gccccagcgc ccaggccagg cctcaactca agcacgaggc
      781 gaaggggctc cttaagcgca aggcctcgaa ctctcccacc cacttccaac ccgaagctcg
      841 ggatcaagaa tcacgtactg cagccaggtg gaagtaattc aaggcacgca agggccataa
      901 cccgtaaaga ggccaggccc gcgggaacca cacacggcac ttacctgtgt tctggcggca
      961 aacccgttgc gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca
     1021 ccctcgggaa aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac
     1081 cttctctagg caccggatca attgccgacc cctcccccca acttctcggg gactgtgggc
     1141 gatgtgcgct ctgcccactg acgggcaccg gagccaattc ccactccttt caagacctag
     1201 aaggtccatt agctgcaaag attcctctct gtttaaaact ttatccatct ttgcacccgg
     1261 gccccctcga gccggcgcca aagtggatct ctgctgtccc tgtaataaac ccgaaaattt
     1321 tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct
     1381 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg
     1441 aatactgcca tttgtctcaa gatctagtta cgccaagctt aaaaaagcac cgactcggtg
     1501 ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa
     1561 acccgggagc accttcgtgg tcggtgtttc gtcctttcca caagatatat aaagccaaga
     1621 aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta
     1681 aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct
     1741 ttgtgtttac agtcaaatta attctaatta tctctctaac agccttgtat cgtatatgca
     1801 aatatgaagg aatcatggga aataggccct ctctgggtcc cctcggggtt gggaggtggg
     1861 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
     1921 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
     1981 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
     2041 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
     2101 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
     2161 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
     2221 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
     2281 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
     2341 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
     2401 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
     2461 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
     2521 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
     2581 aattgtccct catatctcct cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg
     2641 gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
     2701 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
     2761 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat
     2821 gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata
     2881 tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat
     2941 ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg
     3001 ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct
     3061 tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc
     3121 ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg agggatctct
     3181 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     3241 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     3301 ctaaccagag agacccgttt atgtatcgag ctaggcactt aaatacaata tctctgcaat
     3361 gcggcaattc agtggttcgt ccaatccatg tcagacccgt ctgttgcctt cctaataagg
     3421 cacgatcgta ccaccttact tccaccaatc ggcatgcacg gtgctttttc tctccttgta
     3481 aggcatgttg ctaactcatc gttaccatgt tgcaagacta caagagtatt gcataagact
     3541 acattaagct tgcagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     3601 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     3661 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3721 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3781 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3841 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3901 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3961 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4021 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4081 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4141 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4201 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4261 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4321 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4381 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4441 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4501 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4561 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4621 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4681 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4741 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4801 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4861 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4921 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4981 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     5041 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     5101 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     5161 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     5221 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     5281 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     5341 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     5401 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5461 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5521 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5581 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5641 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5701 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5761 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     5821 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     5881 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     5941 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     6001 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     6061 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     6121 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     6181 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     6241 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     6301 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     6361 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtacgtcc
     6421 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     6481 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     6541 gcggagttag gggcgggatg ggcggagtta ggggcgggat agctagagcc agacatgata
     6601 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     6661 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     6721 cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa taagatgaca
     6781 tgaactacta tacgtactgc tagagatttt ccacactgac taaaagggtc tgagggatct
     6841 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
     6901 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
     6961 gtctaaccag agagacccag tacaagcaaa aagcagatct tgtcttcgtt gggagtgaat
     7021 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
     7081 agtcattggt cttaaagtcg acgcggggag gcggcccaaa gggagatccg actcgtctga
     7141 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     7201 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     7261 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
     7321 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     7381 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     7441 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     7501 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     7561 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     7621 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     7681 agaggttgat tgtcgactta acgcgttcag aagaactcgt caagaaggcg atagaaggcg
     7741 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     7801 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     7861 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     7921 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg
     7981 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     8041 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     8101 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     8161 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     8221 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     8281 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg
     8341 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     8401 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     8461 gcggccggag aacctgcgtg caatccatct tgttcaatca tggtggccgt acgtcac
//
