LOCUS       sequence-440869-       14733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BBM-VP16 and WUS in plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14733)
  AUTHORS   Sato Y, Minamikawa MF, Pratama BB, Koyama S, Kojima M, Takebayashi 
            Y, Sakakibara H, Igawa T
  TITLE     Autonomous differentiation of transgenic cells requiring no external
            hormone application: the endogenous gene expression and phytohormone
            behaviors.
  JOURNAL   Front Plant Sci. 2024 Apr 3;15:1308417. doi: 
            10.3389/fpls.2024.1308417. eCollection 2024.
  PUBMED    38633452
REFERENCE   2  (bases 1 to 14733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2024-04-3"; volume: "15:1308417. doi"; pages: " 
            10.3389/fpls.2024.1308417. eCollection 2024"
COMMENT     Sequence Label: pKI_Omega-BBMVP16&WUS
FEATURES             Location/Qualifiers
     source          1..14733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    350..374
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(467..719)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(917..1942)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(1978..2157)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2458..2803
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4629..4862
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     terminator      4879..5127
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     terminator      complement(6717..6969)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7083..7428
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      8254..8506
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8521..8545
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9876..10505
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10934..12007
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12073..12267
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12503..12525)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12611..12751
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12666..12685
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12766..12783)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12937..13525)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13017..13036)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13771..14562)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
       61 ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgcccgaa ttgatcacag
      121 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
      181 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
      241 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
      301 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
      361 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
      421 tgtactgaat taacgccgaa ttgaattatc agcttgcatg ccggtcgatc tagtaacata
      481 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
      541 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
      601 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
      661 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
      721 gcttgactct agggaattaa ttcctgaatc actgcgaccg gccctcccgc gacccagccg
      781 agcgagctta gcgaactgtg gacgagaact gtgccaccaa gcgtaaggcc gttctctcgc
      841 atttgccttg ctaggctcgc gcgagttgct ggctgaggcg ttctcgaaat cagctcttgt
      901 tcggtcggca tctactctat tcctttgccc tcggacgagt gctggggcgt cggtttccac
      961 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
     1021 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
     1081 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
     1141 ggagcatata cgcccggagc cttggcgatc ctgcaagctc cggatgcctc cgctcgaagt
     1201 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
     1261 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
     1321 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
     1381 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
     1441 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
     1501 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
     1561 taagatcggc cgcagcgata gcatccatag cctccgcgac cggctgaaga acagcgggca
     1621 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
     1681 tcaggctctc gctgaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
     1741 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
     1801 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
     1861 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
     1921 cggtgagttc aggctttttc atagggggga ttcgagttga gagtgaatat gagactctaa
     1981 ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc
     2041 tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc
     2101 ttagctcatt aaactccaga aacccgcggc tcagtggctc cttcaacgtt gcggttctgt
     2161 cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc
     2221 ttaattctcc gctcatgatc gataattcgc ggtacccggg gatcaatcaa aggccatgga
     2281 gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg aacagttcat
     2341 acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga
     2401 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
     2461 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
     2521 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
     2581 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     2641 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     2701 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     2761 cccttcctct atataaggaa gttcatttca tttggagaga acacggggga ctctagagga
     2821 tccacaatta ccaacaacaa caaacaacaa acaacattac aattacagat cccatgaact
     2881 cgatgaataa ctggttaggc ttctctctct ctcctcatga tcaaaatcat caccgtacgg
     2941 atgttgactc ctccaccacc agaaccgccg tagatgttgc cggagggtac tgttttgatc
     3001 tggccgctcc ctccgatgaa tcttctgccg ttcaaacatc ttttctttct cctttcggtg
     3061 tcaccctcga agctttcacc agagacaata atagtcactc ccgagattgg gacatcaatg
     3121 gtggtgcatg caataacatt aacaataacg aacaaaatgg accaaagctt gagaatttcc
     3181 tcggccgcac caccacgatt tacaatacca acgagaccgt tgtagatgga aatggcgatt
     3241 gtggaggagg agacggtggt ggtggcggct cactaggcct ttcgatgata aaaacatggc
     3301 tgagtaatca ttcggttgct aatgctaatc atcaagacaa tggtaacggt gcacgaggct
     3361 tgtccctctc tatgaattca tctactagtg atagcaacaa ctacaacaac aatgatgatg
     3421 tcgtccaaga gaagactatt gttgatgtcg tagaaactac accgaagaaa actattgaga
     3481 gttttggaca aaggacgtct atataccgcg gtgttacaag gcatcggtgg acaggtagat
     3541 acgaggcaca tttatgggac aatagttgca aaagagaagg ccagactcgc aaaggaagac
     3601 aagtttatct gggaggttat gacaaagaag aaaaagcagc tagggcttac gatttagccg
     3661 cactaaagta ttggggaacc accactacta ctaacttccc cttgagtgaa tatgagaaag
     3721 aggtagaaga gatgaagcac atgacgaggc aagagtatgt tgcctctctg cgcaggaaaa
     3781 gtagtggttt ctctcgtggt gcatcgattt atcgaggagt aacaaggcat caccaacatg
     3841 gaaggtggca agctaggatc ggaagagtcg ccggtaacaa agacctctac ttgggaactt
     3901 tcggcacaca ggaagaggct gctgaggctt atgacattgc agccattaaa ttcagaggat
     3961 taagcgcagt gactaacttc gacatgaaca gatacaatgt taaagcaatc ctcgagagcc
     4021 cgagtctacc tattggtagt tctgcgaaac gtctcaagga cgttaataat ccggttccag
     4081 ctatgatgat tagtaataac gtttcagaga gtgcaaataa tgttagcggt tggcaaaaca
     4141 ctgcgtttca gcatcatcag ggaatggatt tgagcttatt gcagcaacag caggagaggt
     4201 acgttggtta ttacaatgga ggaaacttgt ctaccgagag tactagggtt tgtttcaaac
     4261 aagaggagga acaacaacac ttcttgagaa actcgccgag tcacatgact aatgttgatc
     4321 atcatagctc gacctctgat gattctgtta ccgtttgtgg aaatgttgtt agttatggtg
     4381 gttatcaagg attcgcaatc cctgttggaa catcggttaa ttacgatccc tttactgctg
     4441 ctgagattgc ttacaacgca agaaatcatt attactatgc tcagcatcag caacaacagc
     4501 agattcagca gtcgccggga ggagattttc cggtggcgat ttcgaataac catagctcta
     4561 acatgtactt tcacggggaa ggtggtggag aaggggctcc aacgttttca gtttggaacg
     4621 acactggggc ccccccgacc gatgtcagcc tgggggacga gctccactta gacggcgagg
     4681 acgtggcgat ggcgcatgcc gacgcgctag acgatttcga tctggacatg ttgggggacg
     4741 gggattcccc agggccggga tttacccccc acgactccgc cccctacggc gctctggata
     4801 tggccgactt cgagtttgag cagatgttta ccgatgccct tggaattgac gagtacggtg
     4861 ggtaggtcga cgagctcata tgaagatgaa gatgaaatat ttggtgtgtc aaataaaaag
     4921 cttgtgtgct taagtttgtg tttttttctt ggcttgttgt gttatgaatt tgtggctttt
     4981 tctaatatta aatgaatgta agatctcatt ataatgaata aacaaatgtt tctataatcc
     5041 attgtgaatg ttttgttgga tctcttctgc agcatataac tactgtatgt gctatggtat
     5101 ggactatgga atatgattaa agataaggaa ttcgcggcct ttcaatttca tggtgaggat
     5161 atgcagtttt ctttgtatat cattcttctt cttctttgta gcttggagtc aaaatcggtt
     5221 ccttcatgta catacatcaa ggatatgtcc ttctgaattt ttatatcttg caataaaaat
     5281 gcttgtacca attgaaacac cagctttttg agttctatga tcactgactt ggttctaacc
     5341 aaaaaaaaaa aaatgtttaa tttacatatc taaaagtagg tttagggaaa cctaaacagt
     5401 aaaatatttg tatattattc gaatttcact catcataaaa acttaaattg caccataaaa
     5461 ttttgtttta ctattaatga tgtaatttgt gtaacttaag ataaaaataa tattccgtaa
     5521 gttaaccggc taaaaccacg tataaaccag ggaacctgtt aaaccggttc tttactggat
     5581 aaagaaatga aagcccatgt agacagctcc attagagccc aaaccctaaa tttctcatct
     5641 atataaaagg agtgacatta gggtttttgt tcgtcctctt aaagcttctc gttttctctg
     5701 ccgtctctct cattcgcgcg acgcaaacga tcttcaggtg atcttctttc tccaaatcct
     5761 ctctcataac tctgatttcg tacttgtgta tttgagctca cgctctgttt ctctcaccac
     5821 agccaatgga gccgccacag catcagcatc atcatcatca agccgaccaa gaaagcggca
     5881 acaacaacaa caacaagtcc ggctctggtg gttacacgtg tcgccagacc agcacgaggt
     5941 ggacaccgac gacggagcaa atcaaaatcc tcaaagaact ttactacaac aatgcaatcc
     6001 ggtcaccaac agccgatcag atccagaaga tcactgcaag gctgagacag ttcggaaaga
     6061 ttgagggcaa gaacgtcttt tactggttcc agaaccataa ggctcgtgag cgtcagaaga
     6121 agagattcaa cggaacaaac atgaccacac catcttcatc acccaactcg gttatgatgg
     6181 cggctaacga tcattatcat cctctacttc accatcatca cggtgttccc atgcagagac
     6241 ctgctaattc cgtcaacgtt aaacttaacc aagaccatca tctctatcat cataacaagc
     6301 catatcccag cttcaataac gggaatttaa atcatgcaag ctcaggtact gaatgtggtg
     6361 ttgttaatgc ttctaatggc tacatgagta gccatgtcta tggatctatg gaacaagact
     6421 gttctatgaa ttacaacaac gtaggtggag gatgggcaaa catggatcat cattactcat
     6481 ctgcacctta caacttcttc gatagagcaa agcctctgtt tggtctagaa ggtcatcaag
     6541 aagaagaaga atgtggtggc gatgcttatc tggaacatcg acgtacgctt cctctcttcc
     6601 ctatgcacgg tgaagatcac atcaacggtg gtagtggtgc catctggaag tatggccaat
     6661 cggaagttcg cccttgcgct tctcttgagc tacgtctgaa ctagtgcatg ccggtcgatc
     6721 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
     6781 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
     6841 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
     6901 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
     6961 tgaacgatct gcttgactct agggaatttc gaccatatgg gagagctcaa gcttagcttg
     7021 agcttggatc agattgtcgt ttcccgcctt cagtttacag tctcagaaga ccaaagggca
     7081 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
     7141 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
     7201 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
     7261 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     7321 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     7381 caagaccctt cctctatata aggaagttca tttcatttgg agagaacacg ggggactctt
     7441 gaccatggaa gcttgaattc gcgaggatcc ataatggtta tttcatctgt atggtcggga
     7501 tcgtcttcga gagtgagaaa aggttcatgg tccgaagaag aagaccaact tttgagggag
     7561 tgcattcaga aatatggtga aggaaaatgg catctaattc cccttagagc tggattgaat
     7621 aggtgcagaa aaagttgtag attaagatgg ttgaattatc tccgtcccga tataaagaga
     7681 ggcgaattta gtcccgatga aattgatctc attctgcgcc tccataggct cttaggcaac
     7741 aggtggtcgc ttattgctgg aagaattccg ggaagaacag caaacgatgt gaagaattta
     7801 tggaacaccc atcttcagaa gaaggtgtct gccatggctt cttcaaggca agataattat
     7861 tggaagggca aagccccaga aatcacggaa aacaccgtcg ttaggcctcg acctcggaga
     7921 ttcttaaagg cctcatcatc tccgacgact ctattgaccg gaaatgctac catggttgcc
     7981 tatgatggtc aactccaaga acatatgacg acacaaccgg aaacaacgtc ggacttgcta
     8041 atggaaaatg tccaacaaaa aaacttaaca accactttgc cttcagcact agaaacaacg
     8101 ccacacgaca atgtgaagtg gtgggaagat gtactctccg acaaggaact caatgaggaa
     8161 ggacaaatct gttggagtga gtttccaact gatatagacc tactgtcaga actgttaagc
     8221 tctgcagatc tggtgaccag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
     8281 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     8341 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     8401 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     8461 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcaaac tatcagtgtt
     8521 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
     8581 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
     8641 gggttcccct cgggatcaaa gtactttaaa gtactttaaa gtactttaaa gtactttgat
     8701 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
     8761 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
     8821 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
     8881 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
     8941 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
     9001 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
     9061 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
     9121 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
     9181 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     9241 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     9301 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     9361 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     9421 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     9481 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     9541 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     9601 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     9661 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     9721 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     9781 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     9841 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     9901 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     9961 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
    10021 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
    10081 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
    10141 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
    10201 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
    10261 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
    10321 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
    10381 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
    10441 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
    10501 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
    10561 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
    10621 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
    10681 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
    10741 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
    10801 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
    10861 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
    10921 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
    10981 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
    11041 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
    11101 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca
    11161 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
    11221 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
    11281 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
    11341 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
    11401 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
    11461 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
    11521 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
    11581 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
    11641 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg
    11701 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
    11761 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
    11821 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
    11881 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
    11941 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
    12001 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
    12061 aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
    12121 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
    12181 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
    12241 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca
    12301 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
    12361 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
    12421 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
    12481 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    12541 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    12601 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
    12661 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    12721 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
    12781 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    12841 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    12901 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    12961 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    13021 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    13081 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    13141 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    13201 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    13261 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    13321 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    13381 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    13441 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    13501 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    13561 tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat ctcccaattt
    13621 gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg
    13681 ctgtgagcaa ttatgtgctt agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg
    13741 cgtcggcttg aacgaatttc tagctagaca ttatttgccg actaccttgg tgatctcgcc
    13801 tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc
    13861 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
    13921 ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta
    13981 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga
    14041 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc
    14101 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag
    14161 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt
    14221 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
    14281 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
    14341 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
    14401 caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc
    14461 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
    14521 tgatagttga gtcgatactt cggcgatcac cgcttccccc atgatgttta actttgtttt
    14581 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
    14641 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaacagtc
    14701 ataacaagcc atgaaaaccg ccactgcgcc gtt
//
