LOCUS       sequence-440940-       13549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CNOT2 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 13549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:CNOT_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..13549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gap             1161..1260
                     /estimated_length=100
     polyA_signal    7944..8025
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(8327..9040,9041..9043,9044..9046))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8330..9040,9041..9043,9044..9046))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8372..8393)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8721..8740
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8980..9001
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10227..10243)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10227..10243)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(10298..10316)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10326..10343)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10326..10342)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10335..10357)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(10483..10938)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10620..10641)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10832..10851
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10965..11069
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11070..11138,11139..11930)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11288..11307)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12101..12689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12590..12609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12843..12860
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12977..12998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13013..13018,13019..13036,13037..13043)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13051..13067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13056..13078
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13075..13091
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13075..13091
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13110..13130
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13112..13130
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcactgttct tcaggcactc gtggttacgg gtttttgttt tgcactcttg cacacacatc
       61 tctcggcctc caaaacaaac acacaactgc acgagggctc gaagccccca ccgaaaaaca
      121 aaaacattat gctaatgagg aaggcacagc tgttcccttc ttcactctct ctctcgttct
      181 ttcaacaaca gtcgcctaat ttctctcgtt cctttaaaga gccgccaagc agaaacagtt
      241 aatgcaaata taagcctgcg ggaaataagg agaattttta caaaatccga gcttcccctc
      301 ctgccaaaat gtgatgtgtg actcacgcat tcgatatttg gtgtaatgag agtgaatcat
      361 tctgctccac accgaacccg atgccacaga ccactcagat gcacaccgag gagactattt
      421 taacagcaca ttacatccag actgtcacag atgcattacc aagagtgtga tttcagtgtc
      481 gaatgcaggt cttaaaggaa aatctgttgc gattgcatgt gcgctaacta gacacgacca
      541 acgaatggga acaatctgtg aaatattata caatcacagg caattaaaag atgtttaaag
      601 ttcaatttag ttttaattaa tatgttatat aaaactaatc tgttttaaat gtatatttat
      661 gattttagct ttagctagcc tcttgaccag cagctgaagt gtccggcaag tacctaaaag
      721 ggagaccaca tgggaaagct aggttgctcc cggaagaggt gttagtaaac cagcaggtct
      781 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct
      841 gtgtgggtcc taatgcccca gtataaacca gcaggtctac ctgtggtctg tgtgggtcct
      901 aatgccccag tataaaccag caggtcaacc tgtagtctgt gtgggtccta atgccccagt
      961 ataaaccagc aggtctacct gtggtctgtg tgggtcctaa tgccccagta taaaccaaca
     1021 ggtcaacctg tgatctgtgt gggtcctaat gccccagtat aaaccagcag gtcaacctgt
     1081 ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg
     1141 gtcctaatgc cccagtataa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 ctacctgtgg tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt
     1321 ctgtgtgggt cctaatgccc cagtataaac cagcaggtct acctgtggtc tgtgtgggtc
     1381 ctaatgcacc agtataaacc agcaggtcaa cctgtggtct gtgtgggttc taatgcccca
     1441 gtataaacca gcaggtcaac ctgtggtctg tgtgggtcca aatgccccag tacaaaccag
     1501 caggtcaacc tgtggtctgt gtgggtccta atgccccagt ataaaccagc aggtcaacct
     1561 gtggtctgtg tgggtcctaa tgccccggta caaaccagca tgtcaacctg tggtctgtgt
     1621 gggtcctaat gccccggtat aaaccagcag atcaacctgt ggtctgtgtg ggtcctaatg
     1681 ccccagtata aaccagcaga tcaacctgtg gtctgtgtgg gtcctaaagt cccaggataa
     1741 accagcatgt caacctgtgg tctgtgtggg tcctaatgcc ccggtataaa ccagcagatc
     1801 aacctgtggt ctgtgtgggt cctaatgccc caggataaac cagcaggtca acctgtggtc
     1861 tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct gtgtgggtcc
     1921 taatgcccca gtatattgat ggagactcta tactgctcgg tgagctccat ctttcggata
     1981 agatgttaaa acaaggtccc gactctctgt ggtggtaaaa aattcccagg atgtccttcc
     2041 taccctggca tcctggacaa atttgctcac tgtcctctgt ccatcatggc ctcctaacca
     2101 tccccatatc ataattggct ttatcactct agctggggcc gaaatcgcat acttccattt
     2161 tatgaggtac gctaaaatca gtatgcgagc caagtaatat gtccgaatac ttccggcgag
     2221 attctagagt gtgcattcga tgcacgctac gctattccat gatgccccgt gagagaattc
     2281 ttgaatcgga gagaagcaac gcaactcatg cgggtaggtt acgtgacgat gacatattgg
     2341 cggatgtagt acgtccaagt tccattcata ctgctcacat tcatatttta tagaatgttc
     2401 ttttcccaca gccaagtagt aagtttaaat tcaaatacag tacctactaa gtagtagtag
     2461 acggtttcgg acgcagcccc tgtctcctct ccaccaatat actggtgtgt agtgtgcggt
     2521 ctggtgcaat atggctgcca tcgcgtcatc caggtggatg ctgcacactg gtggttgatg
     2581 aggagatttt gcccaaaaaa gtgttttgag tttccagaaa agcgcgatat aaatgtaagg
     2641 aattatcatt agcttattat aatggaactt tctggataac ttgtgccaga gctaggggcc
     2701 attaaaactt tgccctgggg tacctcaggg agctgcaagg aagtgctata gggctaatga
     2761 aatgtttggg ggaaaaaaaa aaattttata agtaaaaata aaactaaaat aattaataat
     2821 ttcattgttg aaaattatag taaaattgat aaacaagata agcagcatta gtataaaaaa
     2881 ctaaaaacaa acgtaattta aaataattaa attgttttaa ataaattata caaaataatg
     2941 tttacaacga cacggtggtg cagtaggtag tgctgtcgcc agcaaagcaa gaaggtcgct
     3001 ggttcgagcc tcagctggat cacttgatgt ttctgtgtgg agcattcgcg tgcgtttaac
     3061 cccagattaa taaagagact aagccgaaaa gaaaataaat gtatgaaaat gtttacgtaa
     3121 aatgtaaata aatgcatgat taataaaata tatgaatttt aaagattgag gtagaattgt
     3181 tttattattc acacatttgg ttatttttaa tcagtgacaa cttgtgatgc tctttatatt
     3241 gtttattacg ctactttgaa tattcagtct caaattgcat gtaaatacga cttcaaagca
     3301 gaattagttt aatttaattg cctgtaaaca gtgttgtact ttgatggtga ttgtctgata
     3361 ataatatgat ttcactattt aaaatttgaa aaaaaaaaat ctgaaaaata ctcaccggcc
     3421 actttattag gtacacctgt ctaactgctt gttaacgtaa atttctaatc agccaatcac
     3481 caggcagcaa ctcagtgcat ttaggcatgt agacatggtc aagacgatct gctgcagttc
     3541 aaagcgagca tcagaatgga gaagcaaggt gatttaagtg actttaaacg tgacgtgttt
     3601 gttggtgcca gacgggctgg tctgagtatt tcagaaactg ctgatttact ggggttttca
     3661 cacacaaaca tctctggggt ttacagagaa tggtccaaaa aaagataata tccagtgagc
     3721 ggcagttctg tgggggcaaa tgtcttgttg atgccagagg tcagaggaga atggcctgac
     3781 tggttccagc tgatagaaag gcaacagtaa ttcaaataac cactcgttag aactgaggca
     3841 tgcagaagag cacctttgaa cacaacatgt cgaaccttga ggcgggtggg ctacagcagc
     3901 tcaagaccac atcgggtgcc actcctgtca gctaagaaca ggaaactgag gctacaattc
     3961 gcacaggctc aacataagta gacaatagaa gattgaacat tatgttgcct ggtcagatgg
     4021 atacattcta ccttgtatca acagttcagg ctggtgatgg tggtgtaatg gtgtggggga
     4081 tatattcttg gcacactttg ggcccattag taccaattaa ccatcgtgtc aacgccacag
     4141 cctaactgag tattgttgct gaccatgttc atttcttttt gaccacagtg tacccatctt
     4201 ctgatggcta cttcctgcag gataatgcac catgtcataa agcgtgaatg atctcagact
     4261 ggtttcttga acatgacaat gagttcactg tgctcgaatg acctccacag tcaccagatc
     4321 tcaatccaat agagcgcctt tggggtgtgg tagaatggaa gattcgcatc ctggatgtgc
     4381 agccgacaaa tctgcatgat gctagcatgt caatatggac caaaatctct gaggaatttt
     4441 ccagtacctg gttgaaatgc aaaaggaagg gaatctatgc taaggcaaaa agaggtccaa
     4501 cccagtacta gtggggtgta cataataaag tggccggtga gtgtatattg ttaaggatat
     4561 ctagataatc caaataattt acagcactat ttaaaaaaaa aactttatgt caccaagtct
     4621 gcatttattt gatcacaatt acagtcgaaa aagcaatatc gggatacatt gtttccattt
     4681 aaaataacta tattatattt taaatatttt tgacaatgtt atttctttaa tgccaaagct
     4741 aaattgtatt caatctgaat tttagtgtca catgatcctt cagaaaatat taacacattg
     4801 ctaaaaatgt atattattat caatttggat tttttttttt tttttgtata ttaacctatg
     4861 ctattggtta ctatacaaaa gtattactgt gaatatatgc atatacagaa ccgtatcaag
     4921 cgcggggggt ggttgggtta tgtccccatt tggaaagacc tggaaacatt cataatgact
     4981 aaaattgcac ggaaaaatat aatatctatg agtattgctg atcagcagca caaaaccaga
     5041 actccatatt aataaacagc caatgatgca gtgagtttgt gcatgttttc attcaaactc
     5101 gtgtgattgt gctcaaacac ctgttgtgag cagcagttgc aaacagtaaa cctcaagagg
     5161 agccagtcaa ccttcacaaa cactcaacct ctggctcact cactaaacaa ttcgcgttaa
     5221 tatctaaaat aaaaatttcc agcaccaaac agctctcaga tttcggagta cactgaccaa
     5281 gaggtaagaa gtaacgttac attactagga cgactattgt aagactagat atgtaatatt
     5341 tggaagcgta cgtgttttaa gtgacatata gagtagagga actgatggaa atacgatttg
     5401 cataatgtaa aatgatatat attaaaatat aggctacagt aggtaacgtg gtttacatat
     5461 aacgttactg tgttaatgta gtgctcagtt actcgtgttg acagttatta ttagtgcttg
     5521 ctaatttgtt tgttaatgct tctcttgcgt atttgattac cttcaggcga atctaaaaca
     5581 tatcagatgt agaaacggca gaaaaatgtt gaagaaaata cgagaatagt agcttttgga
     5641 gcacatagaa aagtacatca gataaagcct accgtttaaa cgcagacgcc gctctcgcac
     5701 tgaatgctgg gtactgtagt ccttatattg tatgcttaat gtgcgtctca acgggaaact
     5761 agagttagga aaactacaat ttcctgtgga aaatgtttct gttttcgggg aaacagcgaa
     5821 agaaagatat ggttccgcgt ttttttctta ctttgaataa taaaataaga cctaactaca
     5881 cacttcgctt actcttatgc taatttgaag cactagcatt ctttgataca atgataatca
     5941 tttaaacatc acagttatac tacaaatact atggttaaac tatgtctagt gtagtgtaac
     6001 actatggttg ccattgttat ctttaagttt aagtattatc tgtggtaaag ccatggttgg
     6061 tttttataag aggtaataaa attaacattg tacaattaaa tgactataaa ttaatgtttt
     6121 tttttatttt ttttacacag ctaccttcaa aaagggatca atggtgagga ctgatggaca
     6181 tacattatct gagaaaagaa actaccaggt gacaaacagc atgtttggtg cttcaagaaa
     6241 gaagtttgta gagggggtcg acagtgacta ccatgacgaa aacatgtact acagccagtc
     6301 ttctatgttt ccacatcggt cagaaaaaga tatgctggca tcaccatcta catcaggtca
     6361 gctgtctcag tttggggcaa gtttatacgg gcaacaaagt gcactaggcc ttccaatgag
     6421 ggggatgagc aacaataccc ctcagttaaa tcgcagctta tcacaaggca ctcagttacc
     6481 gagccacgtc acgccaacaa caggggtacc aacaatgtca cttcacacgc ctccatctcc
     6541 aagcaggggt attttgccta tgaatcctag gaatatgatg aaccactccc aggttggtca
     6601 gggcattgga attcctagca ggacaaatag catgagcagt tcagggttag gtagccccaa
     6661 cagaagctcg ccaagcataa tatgtatgcc aaagcagcag ccttctcgac agccttttac
     6721 tgtgaacagt atgtctggat ttggaatgaa caggaatcag gcatttggaa tgaataactc
     6781 cttatcaagt aacattttta atggaacaga cggaagtgaa aatgtgacag gattggacct
     6841 ttcagatttc ccagcattag cagaccgaaa caggagggaa ggaagtggta acccaactcc
     6901 attaataaac cccttggctg gaagagctcc ttatgttgga atggtaacaa aaccagcaaa
     6961 tgaacaatcc caggacttct caatacacaa tgaagatttt ccagcattac caggctccag
     7021 ctataaagat ccaacatcaa gtaatgatga cagtaaatct aatttgaata catctggcaa
     7081 gacaacttca agtacagatg gacccaaatt ccctggagat aaaagttcaa caacacaaaa
     7141 taataaccag cagaaaaaag ggatccaggt gttacctgat ggtcgggtta ctaacattcc
     7201 tcaagggatg gtgacggacc aatttggaat gattggcctg ttaacattta tcagggcagc
     7261 agagacagac ccaggaatgg tacatcttgc attaggaagt gacttaacaa cattaggcct
     7321 caatctgaac tctcctgaaa atctctaccc caaatttgcg tcaccctggg catcttcacc
     7381 ttgtcgacct caagacatag acttccatgt tccatctgag tacttaacga acattcacat
     7441 tagggataag ctggctgcaa taaaacttgg ccgatatggt gaagaccttc tcttctatct
     7501 ctattacatg aatggaggag acgtattaca acttttagct gcagtggagc tttttaaccg
     7561 tgattggaga taccacaaag aagaacgagt atggattacc agggcaccag gcatggagcc
     7621 aacaatgaaa accaatacct atgagagggg aacatattac ttctttgact gtcttaactg
     7681 gaggaaagta gctaaggagt tccatctgga atatgacaaa ttagaagaac ggcctcacct
     7741 gccatccacc ttcaactaca accctgctca gcaagccttc taaatcgatg atgatccaga
     7801 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     7861 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gattgatgag
     7921 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     7981 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     8041 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     8101 ctctacaaat gtggtatggc tgattatgat cctctagatc agatctcttg tttattgcag
     8161 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8221 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     8281 acgtaatacg actcactata gttctagagg ctcgagaggg gccgctttac ttgtacagct
     8341 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
     8401 gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca
     8461 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
     8521 tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt
     8581 cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
     8641 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
     8701 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
     8761 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
     8821 ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca
     8881 cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc
     8941 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
     9001 ggatgggcac caccccggtg aacagctctt cgcccttgct caccatggtc actgtctgct
     9061 ttgctgttgg tctgggctcc tgggtcactg gcttactaat ggagtcttta tgtatgagga
     9121 ctcttatcaa ttgttcttct ataaaggtct gcagtgtttc tgttcgtccc ctacatggac
     9181 acccagagcc tcctaaatac aggagccctg ataactgcac aagtgctcag attccagcag
     9241 ggtggaaatg agataaagtg tgcagatggg gagggggacg tgaatgagag atttgaggga
     9301 tgaaaaggat ggatgaacgc attgaaaata gcccctttca caaaataata ccagtaaatt
     9361 gtcatataat taatgaccag acctttacct gtaaatgatg tgatgttcat atacagaaga
     9421 atgccaacaa tttacagatg attttacaaa ttcttaattt tccttttcta aatggatttg
     9481 ttcacacatt atctcttcaa agcaattgaa ttttctggaa aagattggct gtgtgtgtaa
     9541 aaggtgctac tgaggaattt aatgtgacat ggaatgaagc aaacagcagc ctaagacagg
     9601 atggcaggaa aaatgtcaca tgtttaaaat aagagtgaaa ccaacgacct ggacttatag
     9661 agactgtgtg tccttaggca aagctcttgt acaaacagtg cttgtgactg tgaaattcgg
     9721 ggtttgcctg gattgtgtta aatgtgtgct tggatgtatc ataaaaaaga atcggttctc
     9781 tgtatttgac attttctgta atttatctaa agttacaact ggctatgcct gatttttatc
     9841 actattgaat caatcatttt aaagaatgtt aagtctgctg atttggagag ctcatttaca
     9901 tttattttga atgtctctta tttaacacaa ctgatttaag ctttagatct gcgaagatac
     9961 ggccacgggt gctcttgatc ctgtggctga ttttggactg tgctgctcgc agctgctgat
    10021 gaatcacata cttcctccat tttcttccac tgattgactg ttataatttc cctaatttcc
    10081 aggtcaaggt gctgtgcatt gtggtaatag atgtgacatg attgcattca ttttatgttt
    10141 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
    10201 atggctgatt atgatcctct agatcagatc cactagttct agagcggcca ttaccctgtt
    10261 atccctaggc cgccaccgcg gtggagctcc aattcgccct atagtgagtc gtattacgcg
    10321 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    10381 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    10441 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta agcgttaata
    10501 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
    10561 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
    10621 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
    10681 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
    10741 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
    10801 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
    10861 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
    10921 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    10981 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    11041 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    11101 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    11161 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    11221 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    11281 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    11341 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    11401 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    11461 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    11521 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    11581 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    11641 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    11701 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    11761 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    11821 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    11881 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    11941 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    12001 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    12061 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    12121 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    12181 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    12241 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    12301 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    12361 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    12421 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    12481 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    12541 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    12601 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    12661 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    12721 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    12781 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    12841 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    12901 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    12961 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    13021 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    13081 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    13141 tgggtaccgg gccccccctc gaattaccct gttatcccta tcgagcaaaa tcaatcctaa
    13201 tgtaaaatag tatacgttca tcattataat gcaaacactg tagaactgtt gtgttgtgcc
    13261 ttgcatttca ttctcagttt cttatcgtct gtctcttttt acgacagagc tagaaaaaac
    13321 tagccttcat ttgccatcgc cgtatgcaaa tccactccag aactcaacag gtttgttctg
    13381 cggcgggtgt aagtgtgaac accggcatgt aagtgcgctt gagcaggaga ataaagcgct
    13441 cagagacacc ggaggaatcc tttattttgc gttgcgcctc aggggattcg ctgagccttc
    13501 tcttcatggc cctgctcttc agacacaggt gctccgctgt ttgtgtttt
//
