LOCUS       sequence-440942-       13018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express BEST3 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13018)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 13018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:BEST3_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..13018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    7205..7339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7360..7494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7796..8509,8510..8512,8513..8515))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7799..8509,8510..8512,8513..8515))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7841..7862)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8190..8209
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8449..8470
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9696..9712)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9696..9712)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(9767..9785)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9795..9812)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9795..9811)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9804..9826)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9952..10407)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10089..10110)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10301..10320
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10434..10538
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10539..10607,10608..11399)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10757..10776)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11570..12158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12059..12078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12312..12329
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12446..12467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12482..12487,12488..12505,12506..12512)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12520..12536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12525..12547
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12544..12560
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12544..12560
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12579..12599
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12581..12599
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcactgttct tcaggcactc gtggttacgg gtttttgttt tgcactcttg cacacacatc
       61 tctcggcctc caaaacaaac acacaactgc acgagggctc gaagccccca ccgaaaaaca
      121 aaaacattat gctaatgagg aaggcacagc tgttcccttc ttcactctct ctctcgttct
      181 ttcaacaaca gtcgcctaat ttctctcgtt cctttaaaga gccgccaagc agaaacagtt
      241 aatgcaaata taagcctgcg ggaaataagg agaattttta caaaatccga gcttcccctc
      301 ctgccaaaat gtgatgtgtg actcacgcat tcgatatttg gtgtaatgag agtgaatcat
      361 tctgctccac accgaacccg atgccacaga ccactcagat gcacaccgag gagactattt
      421 taacagcaca ttacatccag actgtcacag atgcattacc aagagtgtga tttcagtgtc
      481 gaatgcaggt cttaaaggaa aatctgttgc gattgcatgt gcgctaacta gacacgacca
      541 acgaatggga acaatctgtg aaatattata caatcacagg caattaaaag atgtttaaag
      601 ttcaatttag ttttaattaa tatgttatat aaaactaatc tgttttaaat gtatatttat
      661 gattttagct ttagctagcc tcttgaccag cagctgaagt gtccggcaag tacctaaaag
      721 ggagaccaca tgggaaagct aggttgctcc cggaagaggt gttagtaaac cagcaggtct
      781 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct
      841 gtgtgggtcc taatgcccca gtataaacca gcaggtctac ctgtggtctg tgtgggtcct
      901 aatgccccag tataaaccag caggtcaacc tgtggtctgt gtgggtccta atgccccagt
      961 atattgatgg agactctata ctgctcggtg agctccatct ttcggataag atgttaaaac
     1021 aaggtcccga ctctctgtgg tggtaaaaaa ttcccaggat gtccttccta ccctggcatc
     1081 ctggacaaat ttgctcactg tcctctgtcc atcatggcct cctaaccatc cccatatcat
     1141 aattggcttt atcactctag ctggggccga aatcgcatac ttccatttta tgaggtacgc
     1201 taaaatcagt atgcgagcca agtaatatgt ccgaatactt ccggcgagat tctagagtgt
     1261 gcattcgatg cacgctacgc tattccatga tgccccgtga gagaattctt gaatcggaga
     1321 gaagcaacgc aactcatgcg ggtaggttac gtgacgatga catattggcg gatgtagtac
     1381 gtccaagttc cattcatact gctcacattc atattttata gaatgttctt ttcccacagc
     1441 caagtagtaa gtttaaattc aaatacagta cctactaagt agtagtagac ggtttcggac
     1501 gcagcccctg tctcctctcc accaatatac tggtgtgtag tgtgcggtct ggtgcaatat
     1561 ggctgccatc gcgtcatcca ggtggatgct gcacactggt ggttgatgag gagattttgc
     1621 ccaaaaaagt gttttgagtt tccagaaaag cgcgatataa atgtaaggaa ttatcattag
     1681 cttattataa tggaactttc tggataactt gtgccagagc taggggccat taaaactttg
     1741 ccctggggta cctcagggag ctgcaaggaa gtgctatagg gctaatgaaa tgtttggggg
     1801 aaaaaaaaaa attttataag taaaaataaa actaaaataa ttaataattt cattgttgaa
     1861 aattatagta aaattgataa acaagataag cagcattagt ataaaaaact aaaaacaaac
     1921 gtaatttaaa ataattaaat tgttttaaat aaattataca aaataatgtt tacaacgaca
     1981 cggtggtgca gtaggtagtg ctgtcgccag caaagcaaga aggtcgctgg ttcgagcctc
     2041 agctggatca cttgatgttt ctgtgtggag cattcgcgtg cgtttaaccc cagattaata
     2101 aagagactaa gccgaaaaga aaataaatgt atgaaaatgt ttacgtaaaa tgtaaataaa
     2161 tgcatgatta ataaaatata tgaattttaa agattgaggt agaattgttt tattattcac
     2221 acatttggtt atttttaatc agtgacaact tgtgatgctc tttatattgt ttattacgct
     2281 actttgaata ttcagtctca aattgcatgt aaatacgact tcaaagcaga attagtttaa
     2341 tttaattgcc tgtaaacagt gttgtacttt gatggtgatt gtctgataat aatatgattt
     2401 cactatttaa aatttgaaaa aaaaaaatct gaaaaatact caccggccac tttattaggt
     2461 acacctgtct aactgcttgt taacgtaaat ttctaatcag ccaatcacca ggcagcaact
     2521 cagtgcattt aggcatgtag acatggtcaa gacgatctgc tgcagttcaa agcgagcatc
     2581 agaatggaga agcaaggtga tttaagtgac tttaaacgtg acgtgtttgt tggtgccaga
     2641 cgggctggtc tgagtatttc agaaactgct gatttactgg ggttttcaca cacaaacatc
     2701 tctggggttt acagagaatg gtccaaaaaa agataatatc cagtgagcgg cagttctgtg
     2761 ggggcaaatg tcttgttgat gccagaggtc agaggagaat ggcctgactg gttccagctg
     2821 atagaaaggc aacagtaatt caaataacca ctcgttagaa ctgaggcatg cagaagagca
     2881 cctttgaaca caacatgtcg aaccttgagg cgggtgggct acagcagctc aagaccacat
     2941 cgggtgccac tcctgtcagc taagaacagg aaactgaggc tacaattcgc acaggctcaa
     3001 cataagtaga caatagaaga ttgaacatta tgttgcctgg tcagatggat acattctacc
     3061 ttgtatcaac agttcaggct ggtgatggtg gtgtaatggt gtgggggata tattcttggc
     3121 acactttggg cccattagta ccaattaacc atcgtgtcaa cgccacagcc taactgagta
     3181 ttgttgctga ccatgttcat ttctttttga ccacagtgta cccatcttct gatggctact
     3241 tcctgcagga taatgcacca tgtcataaag cgtgaatgat ctcagactgg tttcttgaac
     3301 atgacaatga gttcactgtg ctcgaatgac ctccacagtc accagatctc aatccaatag
     3361 agcgcctttg gggtgtggta gaatggaaga ttcgcatcct ggatgtgcag ccgacaaatc
     3421 tgcatgatgc tagcatgtca atatggacca aaatctctga ggaattttcc agtacctggt
     3481 tgaaatgcaa aaggaaggga atctatgcta aggcaaaaag aggtccaacc cagtactagt
     3541 ggggtgtaca taataaagtg gccggtgagt gtatattgtt aaggatatct agataatcca
     3601 aataatttac agcactattt aaaaaaaaaa ctttatgtca ccaagtctgc atttatttga
     3661 tcacaattac agtcgaaaaa gcaatatcgg gatacattgt ttccatttaa aataactata
     3721 ttatatttta aatatttttg acaatgttat ttctttaatg ccaaagctaa attgtattca
     3781 atctgaattt tagtgtcaca tgatccttca gaaaatatta acacattgct aaaaatgtat
     3841 attattatca atttggattt tttttttttt tttgtatatt aacctatgct attggttact
     3901 atacaaaagt attactgtga atatatgcat atacagaacc gtatcaagcg cggggggtgg
     3961 ttgggttatg tccccatttg gaaagacctg gaaacattca taatgactaa aattgcacgg
     4021 aaaaatataa tatctatgag tattgctgat cagcagcaca aaaccagaac tccatattaa
     4081 taaacagcca atgatgcagt gagtttgtgc atgttttcat tcaaactcgt gtgattgtgc
     4141 tcaaacacct gttgtgagca gcagttgcaa acagtaaacc tcaagaggag ccagtcaacc
     4201 ttcacaaaca ctcaacctct ggctcactca ctaaacaatt cgcgttaata tctaaaataa
     4261 aaatttccag caccaaacag ctctcagatt tcggagtaca ctgaccaaga ggtaagaagt
     4321 aacgttacat tactaggacg actattgtaa gactagatat gtaatatttg gaagcgtacg
     4381 tgttttaagt gacatataga gtagaggaac tgatggaaat acgatttgca taatgtaaaa
     4441 tgatatatat taaaatatag gctacagtag gtaacgtggt ttacatataa cgttactgtg
     4501 ttaatgtagt gctcagttac tcgtgttgac agttattatt agtgcttgct aatttgtttg
     4561 ttaatgcttc tcttgcgtat ttgattacct tcaggcgaat ctaaaacata tcagatgtag
     4621 aaacggcaga aaaatgttga agaaaatacg agaatagtag cttttggagc acatagaaaa
     4681 gtacatcaga taaagcctac cgtttaaacg cagacgccgc tctcgcactg aatgctgggt
     4741 actgtagtcc ttatattgta tgcttaatgt gcgtctcaac gggaaactag agttaggaaa
     4801 actacaattt cctgtggaaa atgtttctgt tttcggggaa acagcgaaag aaagatatgg
     4861 ttccgcgttt ttttcttact ttgaataata aaataagacc taactacaca cttcgcttac
     4921 tcttatgcta atttgaagca ctagcattct ttgatacaat gataatcatt taaacatcac
     4981 agttatacta caaatactat ggttaaacta tgtctagtgt agtgtaacac tatggttgcc
     5041 attgttatct ttaagtttaa gtattatctg tggtaaagcc atggttggtt tttataagag
     5101 gtaataaaat taacattgta caattaaatg actataaatt aatgtttttt tttatttttt
     5161 ttacacagct accttcaaaa agggatccat gaccgtgacg tatagctcta aagtcgccaa
     5221 cgccacattc ttcggcttcc acagactcct tttgaaatgg cggggtagta tttataagct
     5281 gctctatcgg gagttcatcg tattcgccgt cctctacact gctattagcc tggtgtatcg
     5341 gctcctgctg accggcgtgc agaagcggta tttcgagaag ttgagtatct attgcgatag
     5401 gtacgcagag cagatcccgg ttactttcgt cctgggtttc tacgtgaccc ttgtcgttaa
     5461 tcgttggtgg aatcaattcg tcaacttacc gtggcccgat cggctgatgt tcttgattag
     5521 tagttccgtg catggctcag atgaacatgg acggctcttg cgccggactc tcatgagata
     5581 tgtgaacctg actagcctgc tgatattccg gtctgtttcc acagccgtct ataagcggtt
     5641 cccgaccatg gatcatgtcg tagaggcggg cttcatgacc accgacgaga gaaagctgtt
     5701 taatcatttg aaatccccac acttaaagta ctgggtgccc tttatttggt tcggcaacct
     5761 ggctaccaag gctcgtaacg agggccgaat acgcgatagc gtcgacttac agtctctgat
     5821 gacggagatg aacagatata ggtcctggtg ttctctgctg tttgggtacg attgggtcgg
     5881 tatccctttg gtatatacac aagtggtgac attagcagtg tacacattct tcttcgcttg
     5941 tttgatcggc aggcaattcc tggacccaac aaaggggtat gccggccacg atttagacct
     6001 gtatatacct atatttacgc tgctgcagtt cttcttttac gccggctggc tgaaagttgc
     6061 tgaacaactc attaatccct tcggcgagga cgacgacgac ttcgagacca attggtgtat
     6121 cgaccgcaac ctgcaagtgt cactgctcgc ggttgatgag atgcatatga gtctccctaa
     6181 aatgaagaaa gatatctatt gggatgactc cgccgcaaga ccaccctata ccctggccgc
     6241 cgccgattat tgtatccctt ctttcttagg ttctaccgtt caaatgggtt tgtccggatc
     6301 agatttccca gatgaagaat ggctctggga ctacgaaaag cacgggcacc gccactctat
     6361 gatcaggcgt gtgaaacgct ttctctccgc acatgagcat ccaagctcac ctcgccggcg
     6421 gagttatcgc cggcaaacct cagattcatc tatgttcttg cctagagacg atttaagtcc
     6481 ggctcgggat ctgctcgacg tcccatccag gaatccacca cgcgcttccc ctacatggaa
     6541 gaagtcttgt tttcctgagg ggtcaccgac actccatttc tctatgggtg aattaagcac
     6601 tatacgcgaa acttctcaaa cctctaccct gcaaagttta acaccccaaa gcagcgtacg
     6661 gacaagccct attaagatgc ctcttgtgcc agaagtgctc attactgccg cagaggctcc
     6721 cgtaccaacc agcggcggtt atcatcacga cagtgcaacg tcaatactct cttccgaatt
     6781 caccggtgtg caaccatcca aaaccgaaca acaacaaggt cctatgggta gcattctcag
     6841 tccttctgag aaagaaactc cacccggcgg gccgagccca caaaccgtga gtgcgtccgc
     6901 agaagagaac atctttaatt gcgaggaaga tccaggggac acattcctca agcggtggag
     6961 cctgcccggg tttctgggaa gcagtcatac atcattaggg aatctgtccc ccgatcctat
     7021 gagttcacaa cccgccttgt tgatcgacac tgagaccagt tccgaaatat ccggaattaa
     7081 tatcgttgca ggaagtcgtg ttagtagcga catgctttac ctcatggaga atcttgatac
     7141 taaagagact gacattatcg aattgaataa agagaccgaa gagagtccta agtagatcga
     7201 tgatgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     7261 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     7321 aagctgcaat aaacaagtta acaacaacaa ttcgatgatg atccagacat gataagatac
     7381 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     7441 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     7501 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     7561 aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatca gatctcttgt
     7621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7741 tctggatcta cgtaatacga ctcactatag ttctagaggc tcgagagggg ccgctttact
     7801 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     7861 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt
     7921 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     7981 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     8041 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     8101 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     8161 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     8221 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     8281 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
     8341 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     8401 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     8461 gctcgaccag gatgggcacc accccggtga acagctcttc gcccttgctc accatggtca
     8521 ctgtctgctt tgctgttggt ctgggctcct gggtcactgg cttactaatg gagtctttat
     8581 gtatgaggac tcttatcaat tgttcttcta taaaggtctg cagtgtttct gttcgtcccc
     8641 tacatggaca cccagagcct cctaaataca ggagccctga taactgcaca agtgctcaga
     8701 ttccagcagg gtggaaatga gataaagtgt gcagatgggg agggggacgt gaatgagaga
     8761 tttgagggat gaaaaggatg gatgaacgca ttgaaaatag cccctttcac aaaataatac
     8821 cagtaaattg tcatataatt aatgaccaga cctttacctg taaatgatgt gatgttcata
     8881 tacagaagaa tgccaacaat ttacagatga ttttacaaat tcttaatttt ccttttctaa
     8941 atggatttgt tcacacatta tctcttcaaa gcaattgaat tttctggaaa agattggctg
     9001 tgtgtgtaaa aggtgctact gaggaattta atgtgacatg gaatgaagca aacagcagcc
     9061 taagacagga tggcaggaaa aatgtcacat gtttaaaata agagtgaaac caacgacctg
     9121 gacttataga gactgtgtgt ccttaggcaa agctcttgta caaacagtgc ttgtgactgt
     9181 gaaattcggg gtttgcctgg attgtgttaa atgtgtgctt ggatgtatca taaaaaagaa
     9241 tcggttctct gtatttgaca ttttctgtaa tttatctaaa gttacaactg gctatgcctg
     9301 atttttatca ctattgaatc aatcatttta aagaatgtta agtctgctga tttggagagc
     9361 tcatttacat ttattttgaa tgtctcttat ttaacacaac tgatttaagc tttagatctg
     9421 cgaagatacg gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca
     9481 gctgctgatg aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc
     9541 ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga ttgcattcat
     9601 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     9661 aaatgtggta tggctgatta tgatcctcta gatcagatcc actagttcta gagcggccat
     9721 taccctgtta tccctaggcc gccaccgcgg tggagctcca attcgcccta tagtgagtcg
     9781 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     9841 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     9901 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa
     9961 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
    10021 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
    10081 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
    10141 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
    10201 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
    10261 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
    10321 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
    10381 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
    10441 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    10501 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    10561 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    10621 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    10681 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    10741 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    10801 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    10861 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    10921 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    10981 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    11041 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    11101 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    11161 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    11221 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    11281 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    11341 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    11401 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    11461 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    11521 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    11581 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    11641 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    11701 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    11761 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    11821 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    11881 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    11941 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    12001 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    12061 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    12121 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    12181 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    12241 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    12301 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    12361 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    12421 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    12481 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    12541 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    12601 aacaaaagct gggtaccggg ccccccctcg aattaccctg ttatccctat cgagcaaaat
    12661 caatcctaat gtaaaatagt atacgttcat cattataatg caaacactgt agaactgttg
    12721 tgttgtgcct tgcatttcat tctcagtttc ttatcgtctg tctcttttta cgacagagct
    12781 agaaaaaact agccttcatt tgccatcgcc gtatgcaaat ccactccaga actcaacagg
    12841 tttgttctgc ggcgggtgta agtgtgaaca ccggcatgta agtgcgcttg agcaggagaa
    12901 taaagcgctc agagacaccg gaggaatcct ttattttgcg ttgcgcctca ggggattcgc
    12961 tgagccttct cttcatggcc ctgctcttca gacacaggtg ctccgctgtt tgtgtttt
//
