LOCUS       sequence-440950-       12570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CCT2 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12570)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 12570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:CCT2_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..12570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    6757..6891
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6912..7046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7348..8061,8062..8064,8065..8067))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7351..8061,8062..8064,8065..8067))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7393..7414)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7742..7761
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8001..8022
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9248..9264)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9248..9264)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(9319..9337)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9347..9364)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9347..9363)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9356..9378)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9504..9959)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9641..9662)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9853..9872
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9986..10090
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10091..10159,10160..10951)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10309..10328)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11122..11710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11611..11630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11864..11881
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11998..12019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12034..12039,12040..12057,12058..12064)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12072..12088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12077..12099
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12096..12112
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12096..12112
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12131..12151
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12133..12151
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcactgttct tcaggcactc gtggttacgg gtttttgttt tgcactcttg cacacacatc
       61 tctcggcctc caaaacaaac acacaactgc acgagggctc gaagccccca ccgaaaaaca
      121 aaaacattat gctaatgagg aaggcacagc tgttcccttc ttcactctct ctctcgttct
      181 ttcaacaaca gtcgcctaat ttctctcgtt cctttaaaga gccgccaagc agaaacagtt
      241 aatgcaaata taagcctgcg ggaaataagg agaattttta caaaatccga gcttcccctc
      301 ctgccaaaat gtgatgtgtg actcacgcat tcgatatttg gtgtaatgag agtgaatcat
      361 tctgctccac accgaacccg atgccacaga ccactcagat gcacaccgag gagactattt
      421 taacagcaca ttacatccag actgtcacag atgcattacc aagagtgtga tttcagtgtc
      481 gaatgcaggt cttaaaggaa aatctgttgc gattgcatgt gcgctaacta gacacgacca
      541 acgaatggga acaatctgtg aaatattata caatcacagg caattaaaag atgtttaaag
      601 ttcaatttag ttttaattaa tatgttatat aaaactaatc tgttttaaat gtatatttat
      661 gattttagct ttagctagcc tcttgaccag cagctgaagt gtccggcaag tacctaaaag
      721 ggagaccaca tgggaaagct aggttgctcc cggaagaggt gttagtaaac cagcaggtct
      781 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct
      841 gtgtgggtcc taatgcccca gtataaacca gcaggtcaac ctgtggtctg tgtgggtcct
      901 aatgccccag tatattgatg gagactctat actgctcggt gagctccatc tttcggataa
      961 gatgttaaaa caaggtcccg actctctgtg gtggtaaaaa attcccagga tgtccttcct
     1021 accctggcat cctggacaaa tttgctcact gtcctctgtc catcatggcc tcctaaccat
     1081 ccccatatca taattggctt tatcactcta gctggggccg aaatcgcata cttccatttt
     1141 atgaggtacg ctaaaatcag tatgcgagcc aagtaatatg tccgaatact tccggcgaga
     1201 ttctagagtg tgcattcgat gcacgctacg ctattccatg atgccccgtg agagaattct
     1261 tgaatcggag agaagcaacg caactcatgc gggtaggtta cgtgacgatg acatattggc
     1321 ggatgtagta cgtccaagtt ccattcatac tgctcacatt catattttat agaatgttct
     1381 tttcccacag ccaagtagta agtttaaatt caaatacagt acctactaag tagtagtaga
     1441 cggtttcgga cgcagcccct gtctcctctc caccaatata ctggtgtgta gtgtgcggtc
     1501 tggtgcaata tggctgccat cgcgtcatcc aggtggatgc tgcacactgg tggttgatga
     1561 ggagattttg cccaaaaaag tgttttgagt ttccagaaaa gcgcgatata aatgtaagga
     1621 attatcatta gcttattata atggaacttt ctggataact tgtgccagag ctaggggcca
     1681 ttaaaacttt gccctggggt acctcaggga gctgcaagga agtgctatag ggctaatgaa
     1741 atgtttgggg gaaaaaaaaa aattttataa gtaaaaataa aactaaaata attaataatt
     1801 tcattgttga aaattatagt aaaattgata aacaagataa gcagcattag tataaaaaac
     1861 taaaaacaaa cgtaatttaa aataattaaa ttgttttaaa taaattatac aaaataatgt
     1921 ttacaacgac acggtggtgc agtaggtagt gctgtcgcca gcaaagcaag aaggtcgctg
     1981 gttcgagcct cagctggatc acttgatgtt tctgtgtgga gcattcgcgt gcgtttaacc
     2041 ccagattaat aaagagacta agccgaaaag aaaataaatg tatgaaaatg tttacgtaaa
     2101 atgtaaataa atgcatgatt aataaaatat atgaatttta aagattgagg tagaattgtt
     2161 ttattattca cacatttggt tatttttaat cagtgacaac ttgtgatgct ctttatattg
     2221 tttattacgc tactttgaat attcagtctc aaattgcatg taaatacgac ttcaaagcag
     2281 aattagttta atttaattgc ctgtaaacag tgttgtactt tgatggtgat tgtctgataa
     2341 taatatgatt tcactattta aaatttgaaa aaaaaaaatc tgaaaaatac tcaccggcca
     2401 ctttattagg tacacctgtc taactgcttg ttaacgtaaa tttctaatca gccaatcacc
     2461 aggcagcaac tcagtgcatt taggcatgta gacatggtca agacgatctg ctgcagttca
     2521 aagcgagcat cagaatggag aagcaaggtg atttaagtga ctttaaacgt gacgtgtttg
     2581 ttggtgccag acgggctggt ctgagtattt cagaaactgc tgatttactg gggttttcac
     2641 acacaaacat ctctggggtt tacagagaat ggtccaaaaa aagataatat ccagtgagcg
     2701 gcagttctgt gggggcaaat gtcttgttga tgccagaggt cagaggagaa tggcctgact
     2761 ggttccagct gatagaaagg caacagtaat tcaaataacc actcgttaga actgaggcat
     2821 gcagaagagc acctttgaac acaacatgtc gaaccttgag gcgggtgggc tacagcagct
     2881 caagaccaca tcgggtgcca ctcctgtcag ctaagaacag gaaactgagg ctacaattcg
     2941 cacaggctca acataagtag acaatagaag attgaacatt atgttgcctg gtcagatgga
     3001 tacattctac cttgtatcaa cagttcaggc tggtgatggt ggtgtaatgg tgtgggggat
     3061 atattcttgg cacactttgg gcccattagt accaattaac catcgtgtca acgccacagc
     3121 ctaactgagt attgttgctg accatgttca tttctttttg accacagtgt acccatcttc
     3181 tgatggctac ttcctgcagg ataatgcacc atgtcataaa gcgtgaatga tctcagactg
     3241 gtttcttgaa catgacaatg agttcactgt gctcgaatga cctccacagt caccagatct
     3301 caatccaata gagcgccttt ggggtgtggt agaatggaag attcgcatcc tggatgtgca
     3361 gccgacaaat ctgcatgatg ctagcatgtc aatatggacc aaaatctctg aggaattttc
     3421 cagtacctgg ttgaaatgca aaaggaaggg aatctatgct aaggcaaaaa gaggtccaac
     3481 ccagtactag tggggtgtac ataataaagt ggccggtgag tgtatattgt taaggatatc
     3541 tagataatcc aaataattta cagcactatt taaaaaaaaa actttatgtc accaagtctg
     3601 catttatttg atcacaatta cagtcgaaaa agcaatatcg ggatacattg tttccattta
     3661 aaataactat attatatttt aaatattttt gacaatgtta tttctttaat gccaaagcta
     3721 aattgtattc aatctgaatt ttagtgtcac atgatccttc agaaaatatt aacacattgc
     3781 taaaaatgta tattattatc aatttggatt tttttttttt ttttgtatat taacctatgc
     3841 tattggttac tatacaaaag tattactgtg aatatatgca tatacagaac cgtatcaagc
     3901 gcggggggtg gttgggttat gtccccattt ggaaagacct ggaaacattc ataatgacta
     3961 aaattgcacg gaaaaatata atatctatga gtattgctga tcagcagcac aaaaccagaa
     4021 ctccatatta ataaacagcc aatgatgcag tgagtttgtg catgttttca ttcaaactcg
     4081 tgtgattgtg ctcaaacacc tgttgtgagc agcagttgca aacagtaaac ctcaagagga
     4141 gccagtcaac cttcacaaac actcaacctc tggctcactc actaaacaat tcgcgttaat
     4201 atctaaaata aaaatttcca gcaccaaaca gctctcagat ttcggagtac actgaccaag
     4261 aggtaagaag taacgttaca ttactaggac gactattgta agactagata tgtaatattt
     4321 ggaagcgtac gtgttttaag tgacatatag agtagaggaa ctgatggaaa tacgatttgc
     4381 ataatgtaaa atgatatata ttaaaatata ggctacagta ggtaacgtgg tttacatata
     4441 acgttactgt gttaatgtag tgctcagtta ctcgtgttga cagttattat tagtgcttgc
     4501 taatttgttt gttaatgctt ctcttgcgta tttgattacc ttcaggcgaa tctaaaacat
     4561 atcagatgta gaaacggcag aaaaatgttg aagaaaatac gagaatagta gcttttggag
     4621 cacatagaaa agtacatcag ataaagccta ccgtttaaac gcagacgccg ctctcgcact
     4681 gaatgctggg tactgtagtc cttatattgt atgcttaatg tgcgtctcaa cgggaaacta
     4741 gagttaggaa aactacaatt tcctgtggaa aatgtttctg ttttcgggga aacagcgaaa
     4801 gaaagatatg gttccgcgtt tttttcttac tttgaataat aaaataagac ctaactacac
     4861 acttcgctta ctcttatgct aatttgaagc actagcattc tttgatacaa tgataatcat
     4921 ttaaacatca cagttatact acaaatacta tggttaaact atgtctagtg tagtgtaaca
     4981 ctatggttgc cattgttatc tttaagttta agtattatct gtggtaaagc catggttggt
     5041 ttttataaga ggtaataaaa ttaacattgt acaattaaat gactataaat taatgttttt
     5101 ttttattttt tttacacagc taccttcaaa aagggatcca tggcgtccct ttcccttgca
     5161 cctgttaaca tctttaaggc aggagctgat gaagagagag cagagacagc tcgtctgact
     5221 tcttttattg gtgccatcgc cattggagac ttggtaaaga gcaccttggg acccaaaggc
     5281 atggacaaaa ttcttctaag cagtggacga gatgcctctc ttatggtaac caatgatggt
     5341 gccactattc taaaaaacat tggtgttgac aatccagcag ctaaagtttt agttgatatg
     5401 tcaagggttc aagatgatga agttggtgat ggcactacct ctgttaccgt tttagcagca
     5461 gaattattaa gggaagcaga atctttaatt gcaaaaaaga ttcatccaca gaccatcata
     5521 gcgggttgga gagaagccac gaaggctgca agagaggcgc tgttgagttc tgcagttgat
     5581 catggttccg atgaagttaa attccgtcaa gatttaatga atattgcggg cacaacatta
     5641 tcctcaaaac ttcttactca tcacaaagac cactttacaa agttagctgt agaagcagtt
     5701 ctcagactga aaggctctgg caacctggag gcaattcata ttatcaagaa gctaggagga
     5761 agtttggcag attcctattt agatgaaggc ttcctgttgg ataaaaaaat tggagtaaat
     5821 caaccaaaac gaattgaaaa tgctaaaatt cttattgcaa atactggtat ggatacagac
     5881 aaaataaaga tatttggttc ccgggtaaga gttgactcta cagcaaaggt tgcagaaata
     5941 gaacatgcgg aaaaggaaaa aatgaaggag aaagttgaac gtattcttaa gcatggaata
     6001 aattgcttta ttaacaggca attaatttat aattatcctg aacagctctt tggtgctgct
     6061 ggtgtcatgg ctattgagca tgcagatttt gcaggtgtgg aacgcctagc tcttgtcaca
     6121 ggtggtgaaa ttgcctctac ctttgatcac ccagaactgg tgaagcttgg aagttgcaaa
     6181 cttatcgagg aagtcatgat tggagaagac aaactcattc acttttctgg ggttgccctt
     6241 ggtgaggctt gtaccattgt tttgcgtggt gccactcaac aaattttaga tgaagcagaa
     6301 agatcattgc atgatgctct ttgtgttctt gcgcaaactg taaaggactc tagaacagtt
     6361 tatggaggag gctgttctga gatgttgatg gctcatgctg tgacacagct tgccaataga
     6421 acaccaggca aagaagctgt tgcaatggag tcttatgcta aagcactgag aatgttgcca
     6481 accatcatag ctgacaatgc aggctatgac agtgcagacc tggtggcaca gctcagggct
     6541 gctcacagtg aaggcaatac cactgctgga ttggatatga gggaaggcac cattggagat
     6601 atggctatcc tgggtataac agaaagtttt caagtgaagc gacaggttct tctgagtgca
     6661 gctgaagcag cagaggtgat tctgcgtgtg gacaacatca tcaaagcggc acccaggaaa
     6721 cgtgtccctg atcaccaccc ctgttaaatc gatgatgatc cagacatgat aagatacatt
     6781 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     6841 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     6901 aattcgatga tgatccagac atgataagat acattgatga gtttggacaa accacaacta
     6961 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     7021 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     7081 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     7141 ctgattatga tcctctagat cagatctctt gtttattgca gcttataatg gttacaaata
     7201 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7261 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tacgtaatac gactcactat
     7321 agttctagag gctcgagagg ggccgcttta cttgtacagc tcgtccatgc cgagagtgat
     7381 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
     7441 gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
     7501 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
     7561 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
     7621 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
     7681 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
     7741 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
     7801 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg
     7861 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
     7921 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
     7981 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
     8041 gaacagctct tcgcccttgc tcaccatggt cactgtctgc tttgctgttg gtctgggctc
     8101 ctgggtcact ggcttactaa tggagtcttt atgtatgagg actcttatca attgttcttc
     8161 tataaaggtc tgcagtgttt ctgttcgtcc cctacatgga cacccagagc ctcctaaata
     8221 caggagccct gataactgca caagtgctca gattccagca gggtggaaat gagataaagt
     8281 gtgcagatgg ggagggggac gtgaatgaga gatttgaggg atgaaaagga tggatgaacg
     8341 cattgaaaat agcccctttc acaaaataat accagtaaat tgtcatataa ttaatgacca
     8401 gacctttacc tgtaaatgat gtgatgttca tatacagaag aatgccaaca atttacagat
     8461 gattttacaa attcttaatt ttccttttct aaatggattt gttcacacat tatctcttca
     8521 aagcaattga attttctgga aaagattggc tgtgtgtgta aaaggtgcta ctgaggaatt
     8581 taatgtgaca tggaatgaag caaacagcag cctaagacag gatggcagga aaaatgtcac
     8641 atgtttaaaa taagagtgaa accaacgacc tggacttata gagactgtgt gtccttaggc
     8701 aaagctcttg tacaaacagt gcttgtgact gtgaaattcg gggtttgcct ggattgtgtt
     8761 aaatgtgtgc ttggatgtat cataaaaaag aatcggttct ctgtatttga cattttctgt
     8821 aatttatcta aagttacaac tggctatgcc tgatttttat cactattgaa tcaatcattt
     8881 taaagaatgt taagtctgct gatttggaga gctcatttac atttattttg aatgtctctt
     8941 atttaacaca actgatttaa gctttagatc tgcgaagata cggccacggg tgctcttgat
     9001 cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat acttcctcca
     9061 ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg tgctgtgcat
     9121 tgtggtaata gatgtgacat gattgcattc attttatgtt tcaggttcag ggggaggtgt
     9181 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc
     9241 tagatcagat ccactagttc tagagcggcc attaccctgt tatccctagg ccgccaccgc
     9301 ggtggagctc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     9361 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     9421 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     9481 tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat attttgttaa aattcgcgtt
     9541 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     9601 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     9661 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     9721 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     9781 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     9841 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     9901 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     9961 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
    10021 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
    10081 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
    10141 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
    10201 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
    10261 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
    10321 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
    10381 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
    10441 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
    10501 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
    10561 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
    10621 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
    10681 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    10741 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    10801 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    10861 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    10921 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    10981 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    11041 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    11101 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    11161 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    11221 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    11281 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    11341 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    11401 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    11461 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    11521 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    11581 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    11641 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    11701 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    11761 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    11821 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    11881 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    11941 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    12001 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    12061 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    12121 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctgggtaccg ggccccccct
    12181 cgaattaccc tgttatccct atcgagcaaa atcaatccta atgtaaaata gtatacgttc
    12241 atcattataa tgcaaacact gtagaactgt tgtgttgtgc cttgcatttc attctcagtt
    12301 tcttatcgtc tgtctctttt tacgacagag ctagaaaaaa ctagccttca tttgccatcg
    12361 ccgtatgcaa atccactcca gaactcaaca ggtttgttct gcggcgggtg taagtgtgaa
    12421 caccggcatg taagtgcgct tgagcaggag aataaagcgc tcagagacac cggaggaatc
    12481 ctttattttg cgttgcgcct caggggattc gctgagcctt ctcttcatgg ccctgctctt
    12541 cagacacagg tgctccgctg tttgtgtttt
//
