LOCUS       sequence-440960-        7323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  10xUAS promoter driving expression of human glucagon-truncated 
            ICAM-zfNrxn1b fusion protein for use in trans-Tango..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Coomer CE, Naumova D, Talay M, Zolyomi B, Snell NJ, Sorkac A, 
            Chanchu JM, Cheng J, Roman I, Li J, Robson D, McLean DL, Barnea G, 
            Halpern ME
  TITLE     Transsynaptic labeling and transcriptional control of zebrafish 
            neural circuits.
  JOURNAL   Nat Neurosci. 2025 Jan;28(1):189-200. doi: 
            10.1038/s41593-024-01815-z. Epub 2024 Dec 19.
  PUBMED    39702668
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-024-01815-z"; journalName: "Nat Neurosci"; date: 
            "2025-01"; volume: "28"; issue: "1"; pages: "189-200"
COMMENT     Sequence Label: UAS:hGCG-ICAM(1235)-  Nrxn1b;cryaa:mCherry
FEATURES             Location/Qualifiers
     source          1..7323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(24..35,36..198)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    714..738
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     742..761
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        742..760
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     785..801
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     786..802
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1340..1369
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    3056..3190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      3941..3950
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3947..4657
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(4092..4110)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4360..4379
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4554..4577
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    4667..4788
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(5180..5367,5368..5379)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5405..5422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5576..6164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5656..5675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6335..7126,7127..7195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6958..6977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7196..7300)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtatctgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt
       61 gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat ctggctagaa
      121 tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt attaaggaag
      181 taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa tctcaaaaaa
      241 cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta tttaattatc
      301 aaactataag acagacaatc taatgccagt acacgctact caaagttgta aaacctcaga
      361 tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc tcttttgggg
      421 ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc gggaagaggt
      481 gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga ctccctggtg
      541 tctgaaacac aggccagatg ggccattcgt acgcttaatt tcgaatatcg atatcaaggg
      601 cccatctcga gtatccggac acctaggtag catgcatggc gccttgtcga ctagctagca
      661 tcccgggaag aattcctcga cggatccacc ggtgtggaat tctgcagata tcaacaagtt
      721 tgtacaaaaa agcaggctta gtaatacgac tcactatagg gcgaattggg taccgggccc
      781 cccctcgagg tcgacggtat cgataagctt aggcctccaa ggcggagtac tgtcctccgg
      841 gctggcggag tactgtcctc cggcaaggtc ggagtactgt cctccgacac tagaggtcgg
      901 agtactgtcc tccgacgcaa ggcggagtac tgtcctccgg gctgcggagt actgtcctcc
      961 ggcaaggtcg gagtactgtc ctccgacact agaggtcgga gtactgtcct ccgacgcaag
     1021 gcggagtact gtcctccggg ctggcggagt actgtcctcc ggcaagggtc gactctagag
     1081 ggtatataat ggatcccatc gcgtctcagc ctcactttga gctcctccac acgaattcct
     1141 gcagcccggg ggatccgcgg ccgccaccat gagtgcactt ctgatcctag cccttgtggg
     1201 agctgctgtt gctcattcac agggcacatt caccagtgac tacagcaagt atctggactc
     1261 caagaaggcc caagagtttg tgcagtggtt gatgaataca ggtaccggca acggaaatgg
     1321 gaacggtaat ggcaccggtg agcaaaagct catttctgaa gaggacttgg ggaacggaaa
     1381 tggcaatggt aacgggaatg gaaacggcaa tggtaacggg aatactagtc agacatctgt
     1441 gtccccctca aaagtcatcc tgccccgggg aggctccgtg ctggtgacat gcagcacctc
     1501 ctgtgaccag cccaagttgt tgggcataga gaccccgttg cctaaaaagg agttgctcct
     1561 gcctgggaac aaccggaagg tgtatgaact gagcaatgtg caagaagata gccaaccaat
     1621 gtgctattca aactgccctg atgggcagtc aacagctaaa accttcctca ccgtgtactg
     1681 gactccagaa cgggtggaac tggcacccct cccctcttgg cagccagtgg gcaagaacct
     1741 taccctacgc tgccaggtgg agggtggggc accccgggcc aacctcaccg tggtgctgct
     1801 ccgtggggag aaggagctga aacgggagcc agctgtgggg gagcccgctg aggtcacgac
     1861 cacggtgctg gtgaggagag atcaccatgg agccaatttc tcgtgccgca ctgaactgga
     1921 cctgcggccc caagggctgg agctgtttga gaacacctcg gccccctacc agctccagac
     1981 ctttgtcctg ccagcgactc ccccacaact tgtcagcccc cgggtcctag aggtggacac
     2041 gcaggggacc gtggtctgtt ccctggacgg gctgttccca gtctcggagg cccaggtcca
     2101 cctggcactg ggggaccaga ggttgaaccc cacagtcacc tatggcaacg actccttctc
     2161 ggccaaggcc tcagtcagtg tgaccgcaga ggacgagggc acccagcggc tgacgtgtgc
     2221 agtaatactg gggaaccaga gccaggagac actgcagaca gtgaccatct acagctttcc
     2281 ggcgcccaac gtgattctga cgaagccaga ggtctcagaa gggaccgagg tgacagtgaa
     2341 gtgtgaggcc caccctagag ccaaggtgac gctgaatggg gttccagccc agccactggg
     2401 cccgagggcc cagctcctgc tgaaggccac cccagaggac aacgggcgca gcttctcctg
     2461 ctctgcaacc ctggaggtgg ccggccagct tatacacaag aaccagaccc gggagcttcg
     2521 tgtcctgtat ggcccccgac tggacgagag ggattgtccg ggaaactgga cgtggccaga
     2581 aaattcccag cagactccaa tgtgccaggc ttgggggaac ccattgcccg agctcaagtg
     2641 tctaaaggat ggcactttcc cactgcccat cggggaatca gtgactgtca ctcgagatct
     2701 tgagggcacc tacctctgtc gggccaggag cactcaaggg gaggtcaccc gcgaggtgac
     2761 cgtgaatgtg ctctcccccc ggtatgaggg gatggtggtg ggcatcgtgg cgggagcggc
     2821 gctctgcatc ctcatcctgc tctacgccat gtacaaatac cgcaaccgag acgaggggtc
     2881 gtaccatgtg gacgagagca gaaactacat ctgcaactcc aacggagcgg cgctgaagga
     2941 gaaaaacact gcagcagagg acagcgggag caagagcaag aagaacaaaa acaaggagta
     3001 ttacgtgtga ttaattaagc caccgcggtg gagctcgaat taattcatcg atgatgatcc
     3061 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     3121 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     3181 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     3241 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     3301 agatcagatc taatctgatc attcaggcgc gccatacaat acatttccta caataatgct
     3361 ctcttttcta ttaatagtgt gcattcagtg caggatgtta tgtatttatt ttctgtagtg
     3421 caaacttttt caaagaattt tccagtctaa gcttttcagt caagaaaaaa aaaagcgatt
     3481 gattatctgt gatatttaaa aaaattagct cttaaatctg gtgaaccaag tggccacaac
     3541 acattaaata ctgacgttct acagccatgt agcttcatct ggtctggttt gttttggcag
     3601 gcacttattt tccggtgata gttggccaca agagtattgt ctgagccatt cagtgctaga
     3661 ctgtcattct caggtcagag tccaccccgc tgattctgct gacaacactg ttcccaccat
     3721 gagataatgc cattccagag agatccattt gtaagcccct ctttctgcag cacaggtata
     3781 taaccagggg tctgcctcca ctaaggccgg cacacatcat ttggggatct ttgtactgta
     3841 ctagcctcta ttagcctcct acttgcgttc agttcacatc ttttgttcat caggtgcaat
     3901 aagttacagt aaggagttac caggtctgac attaccggtc gccaccatgg tgagcaaggg
     3961 cgaggatgac aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     4021 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     4081 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     4141 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     4201 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     4261 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     4321 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     4381 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     4441 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgccgagg tcaagaccac
     4501 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagctgga
     4561 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgcg ccgagggcca
     4621 ccactccacc ggcggcatgg acgagctgta caagtaacag acatgataag atacattgat
     4681 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     4741 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     4801 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggttttttg tttaaacgat
     4861 ctgcgaagat acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc
     4921 gcagctgctg atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt
     4981 tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact
     5041 tccaaaggac caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag
     5101 gcagaataaa taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat
     5161 agcaagggaa aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa
     5221 aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa
     5281 gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac
     5341 agtaatcaag taaaattact caagtacttt acacctctgg ttcttgaggt acctcttccg
     5401 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5461 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5521 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5581 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5641 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5701 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5761 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5821 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5881 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5941 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6001 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6061 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6121 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6181 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6241 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6301 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6361 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6421 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6481 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6541 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6601 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6661 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6721 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6781 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6841 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6901 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6961 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7021 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7081 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7141 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7201 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7261 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaggtaccg
     7321 atc
//
