LOCUS       sequence-440963-       11613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Xla.Tubb2 promoter driving expression of human Arrestin-TEV protease
            fusion protein for use in trans-Tango..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Coomer CE, Naumova D, Talay M, Zolyomi B, Snell NJ, Sorkac A, 
            Chanchu JM, Cheng J, Roman I, Li J, Robson D, McLean DL, Barnea G, 
            Halpern ME
  TITLE     Transsynaptic labeling and transcriptional control of zebrafish 
            neural circuits.
  JOURNAL   Nat Neurosci. 2025 Jan;28(1):189-200. doi: 
            10.1038/s41593-024-01815-z. Epub 2024 Dec 19.
  PUBMED    39702668
REFERENCE   2  (bases 1 to 11613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-024-01815-z"; journalName: "Nat Neurosci"; date: 
            "2025-01"; volume: "28"; issue: "1"; pages: "189-200"
COMMENT     Sequence Label: Xla.Tubb2:hArr-TEV;he1.1:eYFP
FEATURES             Location/Qualifiers
     source          1..11613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(96..107,108..270)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     643..659
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    679..699
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4186..4210
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5439..6146
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GSSLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTSQEAQQWVSGWRLNADSVLWGGHKVFMSKPE
                     EPFQPVKEATQLMN"
     polyA_signal    6198..6332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6394..6418)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(6903..6905,6906..6908,6909..7622)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6948..6969)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7209..7228)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7556..7577
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7860..7877)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7866..8091
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(8092..8112)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8120..8136)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    8152..8286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8725..8912,8913..8924)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8958..8974)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8958..8974)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9011..9031)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9011..9029)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9050..9066)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9063..9085)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9074..9090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9098..9104,9105..9122,9123..9128))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9143..9164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9281..9298)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9452..10040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9532..9551)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10211..11002,11003..11071))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10834..10853
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11072..11176)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11339..11360)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatgccctct gatctgcaaa agacgtgaat atctgttcag acacccatat ccactctgtt
       61 ccacacaggt cagaggtttg tccaggagtt cttgacagag gtgtaaaaag tactcaaaaa
      121 ttttactcaa gtgaaagtac aagtacttag ggaaaatttt actcaattaa aagtaaaagt
      181 atctggctag aatcttactt gagtaaaagt aaaaaagtac tccattaaaa ttgtacttga
      241 gtattaagga agtaaaagta aaagcaagaa agaaaactag agattcttgt ttaagctttt
      301 aatctcaaaa aacattaaat gaaatgcata caaggtttta tcctgcttta gaactgtttg
      361 tatttaatta tcaaactata agacagacaa tctaatgcca gtacacgcta ctcaaagttg
      421 taaaacctca gatttaactt cagtagaagc tgattctcaa aattgttagt gtcaagccta
      481 gctcttttgg ggctgaaaag caatcctgca gtgctgaaaa gcctctcaca ggcagccgat
      541 gcgggaagag gtgtattagt cttgatagag aggctgcaaa tagcaggaaa cgtgagcaga
      601 gactccctgg tgtctgaaac acaggccaga tgggccctcg agcaggaaac agctatgacc
      661 atgattacgc caagctatca actttgtata gaaaagttgt ctagaccctg tctgttcctg
      721 attacatcta gtttgctgcc tgtccggatt attgcctgtt ttttgactac tctcttgtat
      781 cctgtggtgt actttctatt ttggtgtgtt tgactcggcc tgtgacccca accatttatt
      841 tgtctcctgt aactgtaccg cattgactga ttgttactga cccggcctgg cttttaacta
      901 caattcttgt ttccaattct acaaccccac acttggttcc cttgcttggt cagaactctc
      961 tccttgggcc tctcacgtta agacctggcg tcatctgagt agcagagggc tagtcccgat
     1021 gccaaaggcg gctgttatag gcagaagggt gagccgcgac tgggtccttg gtgtctgttc
     1081 tgattttggg acaccctgcc ttacagcccc cctcaagtct ctgattattg ccttgtaacc
     1141 ttgtaaccaa tcagtggaaa tcaagagacc tgcaaagcag gaagtagtgt tctacttatg
     1201 tcactccagt ctttatacat tacatttttg gctaactaac tatattagaa acatttttta
     1261 ttttgcatag ccaatctaca gtatttaccc agtttttatt tttaaactga ataattattt
     1321 taaggataat atctgcctgc agaataaata attcgatata tgccatggat aagcaatatg
     1381 gtgtcttgac aagacagaat ataatttgat attttagaag catggctttt atggacttat
     1441 acacatgtag gtatgatgtg agaacctggt taatggtgtc catttgctgt catgagtttg
     1501 cttggttact gagcacagac tattttatga gctttgccag gtcctgcttt tctcatcctt
     1561 tcaaaacaat gtgttccctt cactccacac agagtaatgt ccatgttcct ggtgtgtgag
     1621 tctcctcttc caaatgcagc atcttctccc cccccatgcc atgtgacact ttcattagca
     1681 tctcctcatg ctttgtgtga attctgctct gccctacccc tccccttatg cagcacaagg
     1741 aagaagggag aaagctgtgt ctgcatatct gcaaaaggct gcctgactga cagtaagaat
     1801 gtctgtctcg aggacctctt cccacacagc tgtaccactg aaatccatat ttattggtag
     1861 tacatctgtt actaacattt taacattaac aacagtgatt acattacctt cagaacattg
     1921 cagtaaggaa cagactcctg tatgctcata gtgtaatacc aggagaatat attgtaaatc
     1981 ttgggtgttg cccactttca gtgatatcag ctttgctttc tgtatttttt aatcatttaa
     2041 tgctccttca tagtaatctt gaaatgcaaa cattttcctt aaggtgggca tagccaaaca
     2101 atattcaaca tcaacacact gggccaaatc aggatgatct gatcaacagg ctaacaatca
     2161 gatcatattt gggagccatt tgccaatgtg gtcctcttcc aacatgatta tccaaactgt
     2221 ctgatagata tatctgcttg atagtcaaat attggtcagg caggccattg agaaggcctc
     2281 atgcatgggc caataagctg ctgattgggt tgcctacttg tggatatttt actagcctgg
     2341 ccagaaaaaa tgttgcttga tgccaatgtc aataattgga aaaaaacatt aaaatataag
     2401 aaggctgtta cttttttccc agaaaaggtg gtaaccataa ctgccaacta attctggaag
     2461 agctgagttg gcagcttata cttggccatt tatcaatcac cacctataag gatgtaccga
     2521 gtctgcgtgc ttattaggta tatgtgagta tgacacaatt ttaaacacta ttatgtctgc
     2581 aagagctgag tcagcagtta tacttggcca tgtattgcca cccataagga tgtactgatt
     2641 caatcatttt tctccaaacc cctaaattca tatgatgtct gccagcttat taggtacagt
     2701 atatgtgagt atgataaaaa tatttgtaat atttaatatt ggttgtgttt tctctctata
     2761 gaaataatac acaatccaaa caaaagtggc ataaatgtga tatattgtaa tgacatgata
     2821 aggaaattac actgatttat tggctattaa tatttctcat ttgcaatcac tatgctgctc
     2881 tggcagcttt tatcagcaca tcagtgctac agaaagaatg gaaccctggc tgtcatgctg
     2941 attctaatgc tcagcagcat tttacacatt ttgctgcatt ttctagctat cagctgatta
     3001 ctcccaggat cccccttgtg ttccactgca gatgtattta ctgatgctta gcttctacca
     3061 acgggcactt acaagtattt ctatgtgggt gtaactagaa agaagatgga ctatgtaaag
     3121 catatgggcc tctagtttgg gcataacctt aatgtacatt tggatgggag gttccagggt
     3181 aaaggttaat ccaatagcac tggtttcccc tagctttgcc acttttaata ggcaaggttt
     3241 cagccctgtc tactcatatg taaattgtga ttgtgatttt atttcacatt tttgttctgt
     3301 atgtttggac agatgtcagt aaaaccattt aactgccccc gtttgttcaa cctcctcgtt
     3361 cttctgctac atccactggt ctttacggaa gcaattaaat aaattaaggg ccatatgatt
     3421 tcctgccctg taaaactggt gtgtgtttgc ggtctgaggc agatgtgctg cttccatttg
     3481 aatacagggg ggatattgaa acactgactc gggaatcccc tggagagtga tttgaggcag
     3541 aaatggatat ggagcagtat catgtgataa aatgaaattg tgaggcagct gcatagggtg
     3601 gcacccatcc ccactgcacc ttccttcccc ctacagctcc cagtgatgga cgagttgtgt
     3661 gggggatggt acctgtcact aatacctttg gagtcatcat taggctaata ttatcaataa
     3721 caaggttaat gtttctatag gcttaatcag cttctcattc gtgttttaat tagaattttt
     3781 tttcattatt tcagtataat attcctttgt aataagcaat aagcacattc ccagcatctt
     3841 tttcaccttc ttggtggtat ctgcctgatg gtgagacaat gcagcatcca tttccattcc
     3901 ctcccttgtc atttgattgg ttcccatccc tacaatgctc agggccagtg gctccaccca
     3961 ccattacatc atctgagccc aggaccacag caagtgaata ggagagagca gcaccctccc
     4021 caaggcgtca caagtgtata aaaggcagca gaaggagcct ctgacagccc agattcttag
     4081 ccggctggaa accatcgggg tgttgcacaa agggcaagta aagcaacaag aaagggccac
     4141 accatccact gattacacca ctgtaagact caacccaatc aagcttcaag tttgtacaaa
     4201 aaagcaggct atgggggaga aacccgggac cagggtcttc aagaagtcga gccctaactg
     4261 caagctcacc gtgtacttgg gcaagcggga cttcgtagat cacctggaca aagtggaccc
     4321 tgtagatggc gtggtgcttg tggaccctga ctacctgaag gaccgcaaag tgtttgtgac
     4381 cctcacctgc gccttccgct atggccgtga agacctggat gtgctgggct tgtccttccg
     4441 caaagacctg ttcatcgcca cctaccaggc cttccccccg gtgccccacc cccccccgcc
     4501 cccccccccg cctgcaggac cggctgctga ggaagctggg ccagcatgcc caccccttct
     4561 tcttcaccat accccagaat cttccatgct ccgtcacact gcagccaggc ccagaggata
     4621 caggaaaggc ctgcggcgta gactttgaga ttcgagcctt ctgtgctaaa tcactagaag
     4681 agaaaagcca caaaaggaac tctgtgcggc tggtgatccg aaaggtgcag ttcgccccgg
     4741 agaaacccgg cccccagcct tcagccgaaa ccacacgcca cttcctcatg tctgaccggt
     4801 ccctgcacct cgaggcttcc ctggacaagg agctgtacta ccatggggag cccctcaatg
     4861 taaatgtcca cgtcaccaac aactccacca agaccgtcaa gaagatcaaa gtctctgtga
     4921 gacagtacgc cgacatctgc ctcttcagca ccgcccagta caagtgtcct gtggctcaac
     4981 tcgaacaaga tgaccaggta tctcccagct ccacattctg taaggtgtac accataaccc
     5041 cactgctcag tgacaaccgg gagaagcggg gtctcgccct ggatgggaaa ctcaagcacg
     5101 aggacaccaa cctggcttcc agcaccatcg tgaaggaggg tgccaacaag gaggtgctgg
     5161 gaatcctggt gtcctacagg gtcaaggtga agctggtggt gtctcgaggc ggggatgtct
     5221 ctgtggagct gccttttgtt cttatgcacc ccaagcccca cgaccacatc cccctcccca
     5281 gaccccagtc agccgctccg gagacagatg tccctgtgga caccaacctc attgaatttg
     5341 ataccaacta tgccacagat gatgacattg tgtttgagga ctttgcccgg cttcggctga
     5401 aggggatgaa ggatgacgac tatgatgatc aactctgcgg atctagcttg tttaagggac
     5461 cacgtgatta caacccgata tcgagcacca tttgtcattt gacgaatgaa tctgatgggc
     5521 acacaacatc gttgtatggt attggatttg gtcccttcat cattacaaac aagcacttgt
     5581 ttagaagaaa taatggaaca ctgttggtcc aatcactaca tggtgtattc aaggtcaaga
     5641 acaccacgac tttgcaacaa cacctcattg atgggaggga catgataatt attcgcatgc
     5701 ctaaggattt cccaccattt cctcaaaagc tgaaatttag agagccacaa agggaagagc
     5761 gcatatgtct tgtgacaacc aacttccaaa ctaagagcat gtctagcatg gtgtcagaca
     5821 ctagttgcac attcccttca tctgatggca tattctggaa gcattggatt caaaccaagg
     5881 atgggcagtg tggcagtcca ttagtatcaa ctagagatgg gttcattgtt ggtatacact
     5941 cagcatcgaa tttcaccaac acaaacaatt atttcacaag cgtgccgaaa aacttcatgg
     6001 aattgttgac aagtcaggag gcgcagcagt gggttagtgg ttggcgatta aatgctgact
     6061 cagtattgtg ggggggccat aaagttttca tgagcaaacc tgaagagcct tttcagccag
     6121 ttaaggaagc gactcaactc atgaatgaat tggtgtactc gtgaccgcgg tggagctcga
     6181 attaattcat cgatgatgat ccagacatga taagatacat tgatgagttt ggacaaacca
     6241 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     6301 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     6361 ttcaggttca gggggaggtg tgggaggttt tttacccagc tttcttgtac aaagtggtca
     6421 accactccag gcatagctag agccactgat gcatctatag agctgaagta aagatagggt
     6481 tgatgcaaat cgatttagaa ttttcaatgt agaaaaaaca aaataaaaag tctattcact
     6541 tgatggatag attagtcaaa caaacataga gtaggcactt cccttgactc tgtaaacttt
     6601 tggatttaac agattgtgca ctccttttag ttaaacacat tagtccctga aaactaagac
     6661 cctaaccata atcccattgt agacccacct actcgggtga atataaagct cgcacagatc
     6721 atctcacaga catcagacct tggcaaagga gcatcctgaa catctctaca cactgaggca
     6781 accctagggc cgccaccatg ctgtgttgta tcagaagaac aaagcctgtg gagaagaatg
     6841 aggaggctga ccaggaactg cagagcacgg tacctagagc tagagaccct cctgtggcta
     6901 caatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     6961 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     7021 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     7081 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
     7141 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     7201 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     7261 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     7321 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     7381 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     7441 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     7501 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     7561 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtataagt
     7621 gagaattctg caggaattgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
     7681 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
     7741 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
     7801 gggcaagttg ggaagacaac ctgtagggca cgaggggggg cccgaaaccc gctgatcagc
     7861 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     7921 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     7981 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     8041 ggattgggaa gacaatagca aggcatgctg gggatgcggt gggctctatg gcaactttat
     8101 tatacatagt tgataattca ctggccgtcg ttttacggta ccatcgatga tgatccagac
     8161 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     8221 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     8281 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     8341 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagat
     8401 cagatctgcg aagatacggc cacgggtgct cttgatcctg tggctgattt tggactgtgc
     8461 tgctcgcagc tgctgatgaa tcacatactt cctccatttt cttccactga ttgactgtta
     8521 taatttccct aatttccagg tcaaggtgct gtgcattgtg gtaatagatg tgacatgacg
     8581 tcacttccaa aggaccaatg aacatgtctg accaatttca tataatgtga aaacgatttt
     8641 cataggcaga ataaataaca tttaaattaa actgggcatc agcgcaattc aattggtttg
     8701 gtaatagcaa gggaaaatag aatgaagtga tctccaaaaa ataagtactt tttgactgta
     8761 aataaaattg taaggagtaa aaagtacttt tttttctaaa aaaatgtaat taagtaaaag
     8821 taaaagtatt gatttttaat tgtactcaag taaagtaaaa atccccaaaa ataatactta
     8881 agtacagtaa tcaagtaaaa ttactcaagt actttacacc tctggttctt gaccccctac
     8941 cttcagcaag cccagcagat ccactagttc tagagcggcc gccaccgcgg tggagctcca
     9001 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     9061 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9121 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9181 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9241 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9301 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9361 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     9901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10081 ggaacgaaaa ctcacgttaa ggcattttgg tcatgagatt atcaaaaagg atcttcacct
    10141 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10201 ggtctgacag ttaccaatgc ttaataagtg aggcacctat ctcagcgatc tgtctatttc
    10261 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10321 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10381 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10441 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10501 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10561 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10621 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10681 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10741 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10801 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10861 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10921 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10981 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    11041 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    11101 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11161 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
    11221 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
    11281 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
    11341 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
    11401 cgtctatcag ggcgatggcc cacgctggct aagaactcat cagcctcccc ggtccatcta
    11461 cccacgtacc aatgcaccaa ttggccacaa tgacggctac tacatggtgc cattccttcc
    11521 tctttatagg aatggagact acctcctgtc caacaatgct cttggatacg agtacgccta
    11581 cctgttggac ccaggtcatt gcacaacacc aga
//
