LOCUS       sequence-440967-        9701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AeBlue visually marked kanamycin-resistant pVS1 plasmid backbone 
            containing the sacB eviction marker and an excisable ccdb/CmR 
            cassette flanked by EcoRV-HindIII restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 8960 to 8994)
  AUTHORS   Pennetti VJ, LaFayette PR, Parrott WA
  TITLE     Single component CRISPR-mediated base-editors for Agrobacterium and 
            their use to develop an improved suite of strains
  JOURNAL   Biodes Res. 2025 March, 7:100001. doi: 10.1016/j.bidere.2025.100001
REFERENCE   2  (bases 1 to 9701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes Res.
            2025 March, 7:100001. doi: 10.1016/j.bidere.2025.100001"
COMMENT     Sequence Label: pVP075
FEATURES             Location/Qualifiers
     source          1..9701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      38..84
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(100..405)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(202..221)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(747..1406)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFLVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1321..1340
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(1460..1466,1467..1484,1485..1490))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        1525..1970
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(1971..2057,2058..3392)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     complement(3499..3518)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3510..4325
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3510..4325
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3583..3602)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4921..5509
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5410..5429
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5663..5680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5695..5835
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5761..5780)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5921..5943
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6179..6373
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6439..7512)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7941..8570)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     promoter        8960..8994
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             9006..9017
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 taaattaggg catcactaat aatagagctc aggacatcaa ataaaacgaa aggctcagtc
       61 gaaagactgg gcctttcgtt ttatctgttg tttaagcttt tatattcccc agaacatcag
      121 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat
      181 aacggaaacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat
      241 atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag
      301 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag
      361 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt
      421 tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct
      481 gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc
      541 ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc
      601 aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggtatac
      661 atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata ctgttatctg
      721 gcttttagta agccggatcc acgcgtttac gccccgccct gccactcatc gcagtactgt
      781 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
      841 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
      901 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
      961 ggattggctg agactaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     1021 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     1081 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     1141 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga
     1201 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     1261 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     1321 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     1381 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga
     1441 tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1501 cctaatgcgg ccgatatcca ggtgcacata tacctgccgt tcactattat ttagtgaaat
     1561 gagatattat gatattttct gaattgtgat taaaaaggca actttatgcc catgcaacag
     1621 aaactataaa aaatacagag aatgaaaaga aacagataga ttttttagtt ctttaggccc
     1681 gtagtctgca aatcctttta tgattttcta tcaaacaaaa gaggaaaata gaccagttgc
     1741 aatccaaacg agagtctaat agaatgaggt cgaaaagtaa atcgcgcggg tttgttactg
     1801 ataaagcagg caagacctaa aatgtgtaaa gggcaaagtg tatactttgg cgtcacccct
     1861 tacatatttt aggtcttttt ttattgtgcg taactaactt gccatcttca aacaggaggg
     1921 ctggaagaag cagaccgcta acacagtaca taaaaaagga gacatgaacg atgaacatca
     1981 aaaagtttgc aaaacaagca acagtattaa cctttactac cgcactgctg gcaggaggcg
     2041 caactcaagc gtttgcgaaa gaaacgaacc aaaagccata taaggaaaca tacggcattt
     2101 cccatattac acgccatgat atgctgcaaa tccctgaaca gcaaaaaaat gaaaaatatc
     2161 aagttcctga gttcgattcg tccacaatta aaaatatctc ttctgcaaaa ggcctggacg
     2221 tttgggacag ctggccatta caaaacgctg acggcactgt cgcaaactat cacggctacc
     2281 acatcgtctt tgcattagcc ggagatccta aaaatgcgga tgacacatcg atttacatgt
     2341 tctatcaaaa agtcggcgaa acttctattg acagctggaa aaacgctggc cgcgtcttta
     2401 aagacagcga caaattcgat gcaaatgatt ctatcctaaa agaccaaaca caagaatggt
     2461 caggttcagc cacatttaca tctgacggaa aaatccgttt attctacact gatttctccg
     2521 gtaaacatta cggcaaacaa acactgacaa ctgcacaagt taacgtatca gcatcagaca
     2581 gctctttgaa catcaacggt gtagaggatt ataaatcaat ctttgacggt gacggaaaaa
     2641 cgtatcaaaa tgtacagcag ttcatcgatg aaggcaacta cagctcaggc gacaaccata
     2701 cgctgagaga tcctcactac gtagaagata aaggccacaa atacttagta tttgaagcaa
     2761 acactggaac tgaagatggc taccaaggcg aagaatcttt atttaacaaa gcatactatg
     2821 gcaaaagcac atcattcttc cgtcaagaaa gtcaaaaact tctgcaaagc gataaaaaac
     2881 gcacggctga gttagcaaac ggcgctctcg gtatgattga gctaaacgat gattacacac
     2941 tgaaaaaagt gatgaaaccg ctgattgcat ctaacacagt aacagatgaa attgaacgcg
     3001 cgaacgtctt taaaatgaac ggcaaatggt acctgttcac tgactcccgc ggatcaaaaa
     3061 tgacgattga cggcattacg tctaacgata tttacatgct tggttatgtt tctaattctt
     3121 taactggccc atacaagccg ctgaacaaaa ctggccttgt gttaaaaatg gatcttgatc
     3181 ctaacgatgt aacctttact tactcacact tcgctgtacc tcaagcgaaa ggaaacaatg
     3241 tcgtgattac aagctatatg acaaacagag gattctacgc agacaaacaa tcaacgtttg
     3301 cgccgagctt cctgctgaac atcaaaggca agaaaacatc tgttgtcaaa gacagcatcc
     3361 ttgaacaagg acaattaaca gttaacaaat aacacctgca gctgtatcta gaatcgcgta
     3421 tgcgagtgtg gcacgaacct tgctgcctgt gatccagatc atgaacaata aaactgtctg
     3481 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct
     3541 ctaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     3601 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag
     3661 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     3721 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     3781 ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc caggtattag
     3841 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     3901 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     3961 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     4021 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     4081 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     4141 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     4201 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     4261 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     4321 tctaacagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     4381 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     4441 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     4501 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     4561 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     4621 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     4681 agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat
     4741 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
     4801 taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc
     4861 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4921 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4981 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5041 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     5101 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5161 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5221 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5281 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5341 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5401 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5461 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5521 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5581 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     5641 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     5701 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     5761 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     5821 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5881 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     5941 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     6001 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     6061 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     6121 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     6181 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     6241 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     6301 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     6361 cgtgctatcc acaggaaaga gagcttttcg acctttttcc cctgctaggg caatttgccc
     6421 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     6481 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     6541 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     6601 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     6661 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     6721 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     6781 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     6841 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     6901 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     6961 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     7021 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     7081 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     7141 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     7201 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     7261 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     7321 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     7381 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     7441 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     7501 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     7561 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     7621 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     7681 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     7741 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     7801 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     7861 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     7921 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     7981 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     8041 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     8101 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     8161 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     8221 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     8281 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     8341 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     8401 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     8461 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     8521 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     8581 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     8641 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     8701 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     8761 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     8821 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     8881 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     8941 gaaggcaccg cgccgcctgt tgacggctag ctcagtccta ggtacagtgc tagctactag
     9001 agattaaaga ggagaaagag tcccatggct tcactggtta aaaaagacat gtgcatcaaa
     9061 atgacgatgg aaggaacagt aaacggtcac catttcaagt gtgtaggaga aggcgaaggc
     9121 aaaccatttg aagggaccca ggtggaaaag atacgcatca ctgaaggtgg gcccttacca
     9181 tttgcgtatg atattttggc cccttgttgc atgtatggca gtaaaacctt cattaagcat
     9241 gtgtcgggta ttccggatta ctttaaggag tcttttcctg agggctttac ctgggaaaga
     9301 acacaaatct tcgaggatgg cggctatctc accatacacc aggacacgag ccttcagggt
     9361 aataatttta ttttcaaagt taatgtcatc ggtgccaact tccctgcaaa cggtcccgtg
     9421 atgcagaaaa aaacagctgg atgggaaccg tgcgttgaga tgctttatcc gcgggacggc
     9481 gtcctgtgtg gtcagagcct gatggccctg aaatgcactg atggcaatca tctgacgtcc
     9541 cacctgcgca ctacctatcg ttctcgcaag ccatccaatg cagttaacat gccggaattt
     9601 cattttgggg atcatcgcat tgagattttg aaagctgaac aaggtaaatt ttatgaacaa
     9661 tacgagtcag cggtggcccg ttactgtgag gcggcaccga g
//
