LOCUS       sequence-440969-        9686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  eforRED visually marked kanamycin-resistant pVS1 plasmid backbone 
            containing the sacB eviction marker and an excisable ccdb/CmR 
            cassette flanked by EcoRV-HindIII restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 5323 to 5357)
  AUTHORS   Pennetti VJ, LaFayette PR, Parrott WA
  TITLE     Single component CRISPR-mediated base-editors for Agrobacterium and 
            their use to develop an improved suite of strains
  JOURNAL   Biodes Res. 2025 March, 7:100001. doi: 10.1016/j.bidere.2025.100001
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes Res.
            2025 March, 7:100001. doi: 10.1016/j.bidere.2025.100001"
COMMENT     Sequence Label: pVP074
FEATURES             Location/Qualifiers
     source          1..9686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1284..1872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1773..1792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2026..2043
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2058..2198
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2124..2143)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2284..2306
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2542..2736
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2802..3875)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4304..4933)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     promoter        5323..5357
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             5369..5380
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      6087..6133
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(6149..6454)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(6251..6270)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(6796..7455)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFLVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7370..7389
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(7509..7515,7516..7533,7534..7539))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        7574..8019
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(8020..8106,8107..9441)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     complement(9548..9567)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(9559..9686,1..688)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             join(9559..9686,1..688)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(9632..9651)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
       61 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
      121 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
      181 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
      241 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
      301 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
      361 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
      421 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
      481 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
      541 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
      601 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
      661 tcatttgatg ctcgatgagt ttttctaaca gcggcggagg aactctttga tccggttcct
      721 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
      781 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
      841 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
      901 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
      961 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     1021 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc
     1081 gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     1141 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     1201 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1261 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1321 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1381 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     1441 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1501 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1561 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1621 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1681 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1741 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1801 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1861 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1921 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1981 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2041 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     2101 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     2161 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     2221 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     2281 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
     2341 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
     2401 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
     2461 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
     2521 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
     2581 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
     2641 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
     2701 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagagcttt tcgacctttt
     2761 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
     2821 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
     2881 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
     2941 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
     3001 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
     3061 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
     3121 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
     3181 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
     3241 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
     3301 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
     3361 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
     3421 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
     3481 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
     3541 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
     3601 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
     3661 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
     3721 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
     3781 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
     3841 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
     3901 cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc
     3961 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
     4021 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
     4081 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
     4141 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
     4201 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
     4261 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
     4321 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
     4381 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
     4441 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
     4501 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
     4561 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
     4621 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
     4681 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
     4741 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
     4801 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
     4861 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
     4921 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
     4981 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
     5041 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
     5101 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
     5161 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
     5221 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
     5281 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tgttgacggc tagctcagtc
     5341 ctaggtacag tgctagctac tagagattaa agaggagaaa gagtcccatg gccgtcatca
     5401 agcagatcat gaagaccaag ctgcacctgg aaggcaccgt caacggccac gacttcacca
     5461 tcgaaggcaa gggcgaaggc aagccgtacg aaggcctgca gcacatgaag atgaccgtca
     5521 ccaagggcgc cccgctgccg ttctccgtcc acatcctgac cccgtcccac atgtacggct
     5581 ccaagccgtt caacaagtac ccggccgaca tcccggacta ccacaagcag tccttcccgg
     5641 aaggcatgtc ctgggaacgc tccatgatct tcgaagacgg cggcgtctgc accgcctcca
     5701 accactcctc catcaacctg caggaaaact gcttcatcta cgacgtcaag ttccacggcg
     5761 tcaacctgcc gccggacggc ccggtcatgc agaagaccat cgccggctgg gaaccgtccg
     5821 tcgaaaccct gtacgtccgc gacggcatgc tgaagtccga caccgccatg gtcttcaagc
     5881 tgaagggcgg cggccaccac cgcgtcgact tcaagaccac ctacaaggcc aagaagccgg
     5941 tcaagctgcc ggaattccac ttcgtcgaac accgcctgga actgaccaag cacgacaagg
     6001 acttcaccac ctgggaccag caggaagccg ccgaaggcca cttctccccg ctgccgaagg
     6061 ccctgccgta atagagctca ggacatcaaa taaaacgaaa ggctcagtcg aaagactggg
     6121 cctttcgttt tatctgttgt ttaagctttt atattcccca gaacatcagg ttaatggcgt
     6181 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggaaaccg
     6241 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg
     6301 ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca
     6361 tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc
     6421 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca
     6481 aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct
     6541 ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg
     6601 gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg
     6661 taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata
     6721 tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa
     6781 gccggatcca cgcgtttacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     6841 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     6901 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     6961 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     7021 gactaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     7081 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     7141 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     7201 ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag
     7261 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     7321 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     7381 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     7441 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgacggat cctaactcaa
     7501 aatccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgcggc
     7561 cgatatccag gtgcacatat acctgccgtt cactattatt tagtgaaatg agatattatg
     7621 atattttctg aattgtgatt aaaaaggcaa ctttatgccc atgcaacaga aactataaaa
     7681 aatacagaga atgaaaagaa acagatagat tttttagttc tttaggcccg tagtctgcaa
     7741 atccttttat gattttctat caaacaaaag aggaaaatag accagttgca atccaaacga
     7801 gagtctaata gaatgaggtc gaaaagtaaa tcgcgcgggt ttgttactga taaagcaggc
     7861 aagacctaaa atgtgtaaag ggcaaagtgt atactttggc gtcacccctt acatatttta
     7921 ggtctttttt tattgtgcgt aactaacttg ccatcttcaa acaggagggc tggaagaagc
     7981 agaccgctaa cacagtacat aaaaaaggag acatgaacga tgaacatcaa aaagtttgca
     8041 aaacaagcaa cagtattaac ctttactacc gcactgctgg caggaggcgc aactcaagcg
     8101 tttgcgaaag aaacgaacca aaagccatat aaggaaacat acggcatttc ccatattaca
     8161 cgccatgata tgctgcaaat ccctgaacag caaaaaaatg aaaaatatca agttcctgag
     8221 ttcgattcgt ccacaattaa aaatatctct tctgcaaaag gcctggacgt ttgggacagc
     8281 tggccattac aaaacgctga cggcactgtc gcaaactatc acggctacca catcgtcttt
     8341 gcattagccg gagatcctaa aaatgcggat gacacatcga tttacatgtt ctatcaaaaa
     8401 gtcggcgaaa cttctattga cagctggaaa aacgctggcc gcgtctttaa agacagcgac
     8461 aaattcgatg caaatgattc tatcctaaaa gaccaaacac aagaatggtc aggttcagcc
     8521 acatttacat ctgacggaaa aatccgttta ttctacactg atttctccgg taaacattac
     8581 ggcaaacaaa cactgacaac tgcacaagtt aacgtatcag catcagacag ctctttgaac
     8641 atcaacggtg tagaggatta taaatcaatc tttgacggtg acggaaaaac gtatcaaaat
     8701 gtacagcagt tcatcgatga aggcaactac agctcaggcg acaaccatac gctgagagat
     8761 cctcactacg tagaagataa aggccacaaa tacttagtat ttgaagcaaa cactggaact
     8821 gaagatggct accaaggcga agaatcttta tttaacaaag catactatgg caaaagcaca
     8881 tcattcttcc gtcaagaaag tcaaaaactt ctgcaaagcg ataaaaaacg cacggctgag
     8941 ttagcaaacg gcgctctcgg tatgattgag ctaaacgatg attacacact gaaaaaagtg
     9001 atgaaaccgc tgattgcatc taacacagta acagatgaaa ttgaacgcgc gaacgtcttt
     9061 aaaatgaacg gcaaatggta cctgttcact gactcccgcg gatcaaaaat gacgattgac
     9121 ggcattacgt ctaacgatat ttacatgctt ggttatgttt ctaattcttt aactggccca
     9181 tacaagccgc tgaacaaaac tggccttgtg ttaaaaatgg atcttgatcc taacgatgta
     9241 acctttactt actcacactt cgctgtacct caagcgaaag gaaacaatgt cgtgattaca
     9301 agctatatga caaacagagg attctacgca gacaaacaat caacgtttgc gccgagcttc
     9361 ctgctgaaca tcaaaggcaa gaaaacatct gttgtcaaag acagcatcct tgaacaagga
     9421 caattaacag ttaacaaata acacctgcag ctgtatctag aatcgcgtat gcgagtgtgg
     9481 cacgaacctt gctgcctgtg atccagatca tgaacaataa aactgtctgc ttacataaac
     9541 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc taggccgcga
     9601 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     9661 caatcaggtg cgacaatcta tcgatt
//
