LOCUS       sequence-440974-        9508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  eforRED visually marked spectinomycin-resistant pVS1 plasmid 
            backbone containing the sacB eviction marker and an excisable 
            ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 5045 to 5079)
  AUTHORS   Pennetti VJ, LaFayette PR, Parrott WA
  TITLE     Single component CRISPR-mediated base-editors for Agrobacterium and 
            their use to develop an improved suite of strains
  JOURNAL   Biodes Res. 2025 March, 7:100001. doi: 10.1016/j.bidere.2025.100001
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes Res.
            2025 March, 7:100001. doi: 10.1016/j.bidere.2025.100001"
COMMENT     Sequence Label: pVP073
FEATURES             Location/Qualifiers
     source          1..9508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1007..1594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1495..1514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1748..1765
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1780..1920
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1846..1865)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2006..2028
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2264..2458
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2524..3597)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4026..4655)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     promoter        5045..5079
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             5091..5102
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      5809..5855
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(5871..6176)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5973..5992)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(6518..7177)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFLVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7092..7111
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(7231..7237,7238..7255,7256..7261))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        7296..7741
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(7742..7828,7829..9163)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     CDS             join(9478..9508,1..761)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
       61 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
      121 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
      181 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
      241 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
      301 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
      361 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
      421 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
      481 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
      541 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
      601 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
      661 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
      721 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
      781 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
      841 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
      901 aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt
      961 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1021 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaccaccg ctaccagcgg tggtttgttt
     1081 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1141 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1201 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1261 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1321 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1381 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1441 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1501 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1561 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1621 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1681 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     1741 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     1801 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     1861 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     1921 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     1981 gcttacagac aagctgtgac cggctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2041 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2101 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2161 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2221 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2281 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2341 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2401 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2461 aaagagagct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2521 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2581 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2641 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     2701 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     2761 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     2821 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     2881 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     2941 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3001 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     3061 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3121 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3181 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3241 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3301 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3361 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3421 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3481 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3541 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
     3601 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt
     3661 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     3721 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     3781 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     3841 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     3901 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     3961 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     4021 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     4081 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
     4141 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
     4201 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
     4261 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
     4321 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
     4381 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
     4441 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
     4501 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
     4561 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
     4621 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
     4681 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
     4741 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
     4801 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gatacgtgcg
     4861 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
     4921 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
     4981 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
     5041 cctgttgacg gctagctcag tcctaggtac agtgctagct actagagatt aaagaggaga
     5101 aagagtccca tggccgtcat caagcagatc atgaagacca agctgcacct ggaaggcacc
     5161 gtcaacggcc acgacttcac catcgaaggc aagggcgaag gcaagccgta cgaaggcctg
     5221 cagcacatga agatgaccgt caccaagggc gccccgctgc cgttctccgt ccacatcctg
     5281 accccgtccc acatgtacgg ctccaagccg ttcaacaagt acccggccga catcccggac
     5341 taccacaagc agtccttccc ggaaggcatg tcctgggaac gctccatgat cttcgaagac
     5401 ggcggcgtct gcaccgcctc caaccactcc tccatcaacc tgcaggaaaa ctgcttcatc
     5461 tacgacgtca agttccacgg cgtcaacctg ccgccggacg gcccggtcat gcagaagacc
     5521 atcgccggct gggaaccgtc cgtcgaaacc ctgtacgtcc gcgacggcat gctgaagtcc
     5581 gacaccgcca tggtcttcaa gctgaagggc ggcggccacc accgcgtcga cttcaagacc
     5641 acctacaagg ccaagaagcc ggtcaagctg ccggaattcc acttcgtcga acaccgcctg
     5701 gaactgacca agcacgacaa ggacttcacc acctgggacc agcaggaagc cgccgaaggc
     5761 cacttctccc cgctgccgaa ggccctgccg taatagagct caggacatca aataaaacga
     5821 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttaagctt ttatattccc
     5881 cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact
     5941 tcttccccga taacggaaac cggcacactg gccatatcgg tggtcatcat gcgccagctt
     6001 tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt
     6061 gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca
     6121 tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca
     6181 ccagcccctg ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc
     6241 catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg
     6301 catggttgtg cttaccagac cggagatatt gacatcatat atgccttgag caactgatag
     6361 ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac
     6421 ttcgggtata catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat
     6481 actgttatct ggcttttagt aagccggatc cacgcgttta cgccccgccc tgccactcat
     6541 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     6601 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     6661 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     6721 aactcaccca gggattggct gagactaaaa acatattctc aataaaccct ttagggaaat
     6781 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     6841 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     6901 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga
     6961 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     7021 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     7081 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     7141 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     7201 atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa
     7261 agcctggggt gcctaatgcg gccgatatcc aggtgcacat atacctgccg ttcactatta
     7321 tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc aactttatgc
     7381 ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag attttttagt
     7441 tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa agaggaaaat
     7501 agaccagttg caatccaaac gagagtctaa tagaatgagg tcgaaaagta aatcgcgcgg
     7561 gtttgttact gataaagcag gcaagaccta aaatgtgtaa agggcaaagt gtatactttg
     7621 gcgtcacccc ttacatattt taggtctttt tttattgtgc gtaactaact tgccatcttc
     7681 aaacaggagg gctggaagaa gcagaccgct aacacagtac ataaaaaagg agacatgaac
     7741 gatgaacatc aaaaagtttg caaaacaagc aacagtatta acctttacta ccgcactgct
     7801 ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac caaaagccat ataaggaaac
     7861 atacggcatt tcccatatta cacgccatga tatgctgcaa atccctgaac agcaaaaaaa
     7921 tgaaaaatat caagttcctg agttcgattc gtccacaatt aaaaatatct cttctgcaaa
     7981 aggcctggac gtttgggaca gctggccatt acaaaacgct gacggcactg tcgcaaacta
     8041 tcacggctac cacatcgtct ttgcattagc cggagatcct aaaaatgcgg atgacacatc
     8101 gatttacatg ttctatcaaa aagtcggcga aacttctatt gacagctgga aaaacgctgg
     8161 ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat tctatcctaa aagaccaaac
     8221 acaagaatgg tcaggttcag ccacatttac atctgacgga aaaatccgtt tattctacac
     8281 tgatttctcc ggtaaacatt acggcaaaca aacactgaca actgcacaag ttaacgtatc
     8341 agcatcagac agctctttga acatcaacgg tgtagaggat tataaatcaa tctttgacgg
     8401 tgacggaaaa acgtatcaaa atgtacagca gttcatcgat gaaggcaact acagctcagg
     8461 cgacaaccat acgctgagag atcctcacta cgtagaagat aaaggccaca aatacttagt
     8521 atttgaagca aacactggaa ctgaagatgg ctaccaaggc gaagaatctt tatttaacaa
     8581 agcatactat ggcaaaagca catcattctt ccgtcaagaa agtcaaaaac ttctgcaaag
     8641 cgataaaaaa cgcacggctg agttagcaaa cggcgctctc ggtatgattg agctaaacga
     8701 tgattacaca ctgaaaaaag tgatgaaacc gctgattgca tctaacacag taacagatga
     8761 aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg tacctgttca ctgactcccg
     8821 cggatcaaaa atgacgattg acggcattac gtctaacgat atttacatgc ttggttatgt
     8881 ttctaattct ttaactggcc catacaagcc gctgaacaaa actggccttg tgttaaaaat
     8941 ggatcttgat cctaacgatg taacctttac ttactcacac ttcgctgtac ctcaagcgaa
     9001 aggaaacaat gtcgtgatta caagctatat gacaaacaga ggattctacg cagacaaaca
     9061 atcaacgttt gcgccgagct tcctgctgaa catcaaaggc aagaaaacat ctgttgtcaa
     9121 agacagcatc cttgaacaag gacaattaac agttaacaaa taacacctgc agctgtatct
     9181 agaatcgcgt atgcgagtgt ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     9241 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     9301 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     9361 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     9421 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     9481 ggggaagcgg tgatcgccga agtatcga
//
