LOCUS       sequence-440995-        9509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  myr-AKT2 gene expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: pdest myr-AKT2 Neo
FEATURES             Location/Qualifiers
     source          1..9509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    229..250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(265..270,271..288,289..295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     327..343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     327..343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     362..382
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        364..382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        410..636
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             637..817
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    864..989
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1482..1715
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1900..1944
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2211..2327
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2350..2653
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2654..2857
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2808..2828
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2854..2878
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2962..2986
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2992..3033
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             3049..3075
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(4523..4547)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4578..5166
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4631..4651)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5049..5060)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5180..5679
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5215..5238)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5612..5631
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5700..6299
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5700..5719)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6196..6216
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6437..6670
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6742..6863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6779..6798)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6833..6852
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6903..7038
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7058..7077)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7059..7077)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7087..7104)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7087..7103)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7096..7118)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7245..7700
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7332..7351)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7542..7563
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7726..7830
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7831..7899,7900..8691)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8049..8068)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8862..9450
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9351..9370
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
      361 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
      421 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
      481 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
      541 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
      601 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
      661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      781 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      841 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      901 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      961 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1021 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1081 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1141 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1201 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1261 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1321 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1381 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1441 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1501 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1561 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1621 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1681 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1741 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1801 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1861 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1921 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1981 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2041 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2101 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2161 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga
     2221 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2281 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2341 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2401 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2461 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2521 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2581 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2641 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2701 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2761 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2821 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2881 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agtccagtgt
     2941 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggcttcac catggggagc
     3001 agcaagagca agcccaagga ccccagccag cgcgggggag aattcatgta cccttatgat
     3061 gtgccagatt atgcctctaa tgaggtatct gtcatcaaag aaggctggct ccacaaacgt
     3121 ggtgaataca tcaagacctg gaggccacgg tacttccttc tgaagagtga tggatctttc
     3181 attgggtata aggagaggcc cgaggcccct gaccagacct taccccccct gaacaatttc
     3241 tctgtagcag aatgccagct gatgaagact gagaggccac gacccaacac ctttgtcata
     3301 cgctgcctgc agtggaccac agtcatcgag aggaccttcc atgtagactc tccagatgag
     3361 agggaagagt ggatgcgggc tatccagatg gtcgccaaca gtctgaagca gcggggccca
     3421 ggtgaggacg ccatggatta caagtgtggc tcccccagtg actcttccac atctgagatg
     3481 atggaggtag ctgtcaacaa ggcacgggcc aaagtgacca tgaatgactt cgattatctc
     3541 aaactcctcg gcaagggcac cttcggcaag gtcattctgg ttcgagagaa ggccactggc
     3601 cgctattatg ccatgaagat cctgcgcaag gaggtcatca ttgcaaagga tgaagtcgcc
     3661 cacacagtca cagagagccg ggttctgcag aataccaggc accccttcct tacagccctc
     3721 aagtatgcct tccagaccca tgaccgccta tgctttgtga tggagtatgc caacgggggt
     3781 gagctgtttt tccacctctc tcgggagcga gtcttcacgg aggatcgggc gcgcttttat
     3841 ggagcagaga ttgtgtcagc tctggagtat ttgcactcga gagatgtggt gtaccgtgac
     3901 atcaagctgg aaaaccttat gttggacaaa gatggccaca tcaagatcac tgactttggc
     3961 ttgtgcaaag agggcatcag tgatggagcc accatgaaaa ccttctgtgg taccccggag
     4021 tacttggcgc ctgaggtgct agaggacaat gactatgggc gagcagtgga ctggtggggg
     4081 ctgggtgtgg tcatgtatga gatgatgtgt ggccgcctgc cattctacaa ccaggaccac
     4141 gagcgcctct ttgagctcat tcttatggag gagatccgct tcccgcgcac actcgggcca
     4201 gaggccaagt ccctgctggc tggactgctg aagaaggacc caaagcagag gctcggcgga
     4261 ggtcccagtg atgcgaagga ggtcatggag catagattct tcctcagcat caactggcag
     4321 gacgtggtac agaaaaagct cctgccaccc ttcaaacctc aggtcacttc agaagtggac
     4381 acaaggtact ttgatgacga gttcaccgcc cagtccatca caatcacacc cccagaccga
     4441 tatgacagcc tggacccgct ggaactggac cagcggacgc acttccccca gttctcctac
     4501 tcagccagca tccgagagta acacccagct ttcttgtaca aagtggttga tatccagcac
     4561 agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4621 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4681 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4741 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4801 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4861 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4921 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     4981 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5041 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5101 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5161 ccccgcctgg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     5221 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     5281 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     5341 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     5401 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     5461 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     5521 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     5581 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     5641 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     5701 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     5761 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     5821 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     5881 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     5941 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     6001 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6061 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     6121 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     6181 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     6241 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     6301 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatctcgag
     6361 ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6421 aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt
     6481 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6541 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6601 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6661 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     6721 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     6781 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6841 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     6901 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6961 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     7021 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     7081 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7141 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     7201 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     7261 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7321 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     7381 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     7441 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     7501 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     7561 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     7621 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     7681 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7741 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7801 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     7861 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     7921 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7981 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8041 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     8101 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8161 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8221 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8281 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8341 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     8401 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8461 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8521 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     8581 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     8641 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     8701 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     8761 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     8821 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8881 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8941 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9001 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9061 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9121 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9181 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9241 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9301 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9361 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9421 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9481 ctggcctttt gctggccttt tgctcacat
//
