LOCUS       sequence-441013-        8610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression vector of myc-Flag tagged MMS22L siRNA 
            resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   O'Donnell L, Panier S, Wildenhain J, Tkach JM, Al-Hakim A, Landry 
            MC, Escribano-Diaz C, Szilard RK, Young JT, Munro M, Canny MD, Kolas
            NK, Zhang W, Harding SM, Ylanko J, Mendez M, Mullin M, Sun T, 
            Habermann B, Datti A, Bristow RG, Gingras AC, Tyers MD, Brown GW, 
            Durocher D
  TITLE     The MMS22L-TONSL complex mediates recovery from replication stress 
            and homologous recombination.
  JOURNAL   Mol Cell. 2010 Nov 24;40(4):619-31. doi: 
            10.1016/j.molcel.2010.10.024. Epub 2010 Nov 4.
  PUBMED    21055983
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2010.10.024"; journalName: "Mol Cell"; date: 
            "2010-11-24- 24"; volume: "40"; issue: "4"; pages: "619-31"
COMMENT     Sequence Label: pMMS22L-siR3
FEATURES             Location/Qualifiers
     source          1..8610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1103..1111
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(2260..2271)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     CDS             4776..4805
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4824..4847
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4867..4883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4867..4883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4894..5516
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5093..5112)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      complement(5665..6253)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5745..5764)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6582..6629
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6654..6673
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6861..7655)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6861..7655)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6972..6991)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7582..7601
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7690..8047)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7704..7839
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        7856..8047
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8004..8024
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(8049..8153)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8180..8608
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8267..8286)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8477..8498
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagaactgt tctgctgcat cgacgttcct gactgacagc ttagagctgg
     1081 agctggggac ggaatggtgc aaacctcctt acttttcttg tgctgttgac aacagaggag
     1141 gaggaaaaca tttttctgga gaatcctacc tctgcagcgg agcccttaag cgattgattt
     1201 tgaatcttga ccctttacca actaattttg aagaagatac cttggaaata tttggcattc
     1261 agtgggttac tgaaacagca ttagtgaatt catctagaga actctttcat ttattcaggc
     1321 aacaactgta caacttggaa accttgttac agtccagttg tgattttggg aaggtatcaa
     1381 ctctacactg caaagcagac aatattaggc agcagtgtgt actatttctc cattatgtta
     1441 aagttttcat cttcaggtat ctgaaagtac agaatgctga gagtcatgtt cctgtccatc
     1501 cttatgaggc tttggaggct cagcttccct cagtgttgat tgatgagctt catggattac
     1561 tcttgtatat tggacaccta tctgaacttc ccagtgttaa tataggagca tttgtaaatc
     1621 aaaaccagat taagcttttt ccaccgtcat ggcatttatt acatctccac ttggatatac
     1681 attggctggt gctagaaatt ctttacatgc tgggtgaaaa attgaaacaa gttgtatatg
     1741 gtcatcagtt tatgaatctg gcaagtgaca atttaaccaa catcagccta tttgaagaac
     1801 attgtgaaac tctcctttgt gatttaataa gcctgtcact caacaggtac gacaaggtta
     1861 ggtcttctga atcattaatg agtgaccagt gtccatgttt atgcattaaa gaattatggg
     1921 ttctacttat tcatcttcta gaccacagaa gtaaatggtt tgtctcggaa tcattttgga
     1981 actggttgaa taaactactt aaaacactgc ttgaaaaatc aagtgaccga agaagatcct
     2041 ctatgcctgt aatccagtcc agggatccat taggttttag ttggtggatt attactcatg
     2101 tagcatcatt ttacaagttt gatcgccatg gagtaccaga tgaaatgaga aaagtggaat
     2161 caaattggaa ctttgtagaa gaactgctga aaaagtccat cagtgttcag ggtgtcattc
     2221 tagaagaaca attacgaatg tatcttcact gttgtttgac actttgtgat ttctgggagc
     2281 caaacattgc aattgttacc attttatggg aatattatag taagaacctg aatagttcct
     2341 tcagtatttc ttggcttcct tttaaaggcc ttgctaatac catgaagtca cccttgtcta
     2401 tgcttgaaat ggtgaaaacg tgttgctgtg acaaacaaga tcaggaacta tataaatcca
     2461 gcagtagtta tactattttt ctttgtattc tggcaaaagt tgttaaaaaa gcaatgaaga
     2521 gcaatggccc tcatccttgg aaacaagtca aaggaagaat atattcaaaa ttccatcaaa
     2581 aaagaatgga agaactaact gaagttggtc tacagaactt ttttagcctt tttctactgt
     2641 tagcagctgt tgcagaggta gaagatgttg caagtcatgt tttagacctc ctgaatttcc
     2701 tcaagcctgc ttttgtaacg tctcagagag ccctcatttg gaagggtcac atggccttcc
     2761 tcttgatgta tgcccagaaa aatctggaca ttggtgtttt ggctgagaaa ttttcatgtg
     2821 ctttccggga gaaagcaaag gaattcttgg tgtctaagaa tgaggaaatg gtacagagac
     2881 agactatctg gacccttctt tccatataca ttgatggtgt tcaagaagtg tttgagacca
     2941 gctattgctt gtatccttcc catgaaaaac tgcttaatga tggatttagt atgcttctgc
     3001 gagcatgtcg agaatctgaa cttaggacag tattgagctt cctacaagct gttctggcca
     3061 gaatcaggag tatgcatcaa caattgtgtc aggaacttca aagggacaat gtggacctat
     3121 ttgtacagtc ttcattatcg gctaaagagc gccaccttgc tgcagttgcc agtgcactgt
     3181 ggagacattt cttttcattt ttgaagagtc agagaatgtc acaggtagtg cctttctcac
     3241 aacttgcgga tgcagctgca gactttactt tgctagcaat ggacatgcca agcacagctc
     3301 catcagattt tcagcctcag ccagttatat caattattca actttttggt tgggatgata
     3361 tcatctgccc tcaagttgta gcaagatatt taagtcatgt cctacaaaat agcacattat
     3421 gtgaagcact ttctcattca ggctatgtat cttttcaagc cttaaccgta agatcatgga
     3481 ttcgttgtgt tttgcaaatg tatattaaaa acctctctgg gcctgatgat ttgctcatag
     3541 ataaaaatct ggaagaggca gttgaaaaag agtacatgaa acagttggtc aaactgacaa
     3601 gattactatt taatctctca gaagtaaaga gtattttctc aaaggcccaa gttgaatatt
     3661 tatccatctc agaagaccct aaaaaagcac ttgttcgatt ctttgaggct gttggtgtaa
     3721 cttacgggaa cgtccagaca ctttctgata aatctgccat ggtcacaaag tccttggaat
     3781 accttggtga agtattaaaa tatattaagc cttatttggg aaaaaaagtt ttcagtgcag
     3841 ggctgcagct gacttatgga atgatgggaa ttcttgtgaa atcatgggca caaatctttg
     3901 ccacttctaa agcccaaaaa ttactattcc ggatcataga ttgtttactg ctgccacatg
     3961 cagtattaca gcaagagaag gaactgcctg cacctatgtt gtcagcaatt cagaaaagtc
     4021 ttcctttgta tctccagggc atgtgtatcg tgtgttgtca atctcaaaat ccgaatgcct
     4081 atttgaatca attgctaggg aatgttattg agcagtatat tgggcgattt cttccagctt
     4141 caccatatgt ttcagatctt ggacaacatc ctgttttgct ggcattgaga aacacagcca
     4201 ctattccacc aatatcatct ctaaagaaat gcattgtgca agtcataagg aaatcctacc
     4261 ttgagtataa ggggtcctca cctcctcctc gcttagcatc cattctggcc ttcatcctcc
     4321 aactcttcaa ggaaactaac acagacattt atgaagttga actactcctc cctggcattt
     4381 taaaatgctt ggtgttagtc agtgaaccac aagttaaaag gctggccaca gagaacctgc
     4441 aatacatggt aaaagcctgc caagtggggt cagaagaaga accttcctcc cagctgactt
     4501 ctgtgtttag gcagtttatc caggattatg gtatgaggta ctattaccag gtttacagca
     4561 ttttagaaac agtagcaaca ttggaccagc aggttgtcat ccacttgatt tctaccctta
     4621 ctcagtctct gaaggattca gagcagaaat ggggccttgg caggaatata gcacaaaggg
     4681 aagcctatag caaacttttg tctcaccttg gacagatggg acaagatgag atgcagagac
     4741 tggaaaatga taatactacg cgtacgcggc cgctcgagca gaaactcatc tcagaagagg
     4801 atctggcagc aaatgatatc ctggattaca aggatgacga cgataaggtt taaacggccg
     4861 gccgcggtca tagctgtttc ctgaacagat cccgggtggc atccctgtga cccctcccca
     4921 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     4981 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     5041 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     5101 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     5161 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     5221 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     5281 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     5341 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag
     5401 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc
     5461 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt
     5521 acgcggccag cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5581 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5641 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5701 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5761 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5821 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5881 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5941 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6001 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6061 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6121 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6181 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6241 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6301 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6361 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat
     6421 ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     6481 gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     6541 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     6601 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
     6661 cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc
     6721 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     6781 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     6841 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     6901 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     6961 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcgatc cgccacaccc
     7021 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     7081 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     7141 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     7201 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     7261 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     7321 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     7381 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     7441 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     7501 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     7561 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     7621 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     7681 tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     7741 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     7801 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     7861 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     7921 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     7981 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc
     8041 gcctcaggac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8101 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8161 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8221 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     8281 ttcccttcct ttctcgccac gttcgccgac tttccccgtc aagctctaaa tcgggggctc
     8341 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     8401 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8461 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8521 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     8581 ctgatttaac aaaaatttaa cgcgaatttt
//
