LOCUS       sequence-441014-       10334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized sRGN3.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Seo SY, Min S, Lee S, Seo JH, Park J, Kim HK, Song M, Baek D, Cho 
            SR, Kim HH
  TITLE     Massively parallel evaluation and computational prediction of the 
            activities and specificities of 17 small Cas9s.
  JOURNAL   Nat Methods. 2023 Jul;20(7):999-1009. doi: 
            10.1038/s41592-023-01875-2. Epub 2023 May 15.
  PUBMED    37188955
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-01875-2"; journalName: "Nat Methods"; date: 
            "2023-07"; volume: "20"; issue: "7"; pages: "999-1009"
COMMENT     Sequence Label: pLenti6.3-sRGN3.1-BlastR
FEATURES             Location/Qualifiers
     source          1..10334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1079..1312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1497..1541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1820..2123
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2124..2327
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2278..2298
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2324..2348
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2399..2417
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2425..2434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2437..2457
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5656..5676
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5683..5706
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5716..5772
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5773..6171
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6196..6784
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6249..6269)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6667..6678)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6855..7088
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7160..7281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7197..7216)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7251..7270
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7321..7456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7477..7495)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7505..7522)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7505..7521)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7514..7536)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7663..8118
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7750..7769)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7960..7981
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8144..8248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8249..8317,8318..9109)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8467..8486)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9280..9868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9769..9788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10022..10039
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10156..10177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10192..10197,10198..10215,10216..10222)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10230..10246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10235..10257
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10254..10270
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10254..10270
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10289..10309
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10291..10309
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
      961 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1021 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1081 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1141 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1201 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1261 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1321 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1381 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1441 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1501 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1561 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1621 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1681 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     1741 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     1801 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1861 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1921 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1981 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2041 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2101 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2161 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2221 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2281 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     2341 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactta
     2401 atacgactca ctatagggag agccgccacc atggcaccga agaagaagcg taaagtcgga
     2461 atccacggag ttcctgcggc aaatcaaaag ttcatcctgg gcctggatat cggcatcacc
     2521 agcgtgggct acggcctgat cgactacgag acaaagaaca tcattgatgc cggcgtgcgg
     2581 ctgtttccag aagccaacgt ggaaaacaac gagggcagac ggtccaagcg gggatctaga
     2641 agactgaagc gaagaagaat ccacagactg gaacgggtga agctgctgct gaccgagtac
     2701 gacctgatca acaaggagca gatccctaca agcaacaacc cttatcaaat ccgggtgaag
     2761 ggcctgagtg agatcctgag caaggacgag ctggccatcg ccctgctgca cctggctaaa
     2821 agaagaggca tccacaacgt cgatgtagcc gccgacaagg aagaaacagc cagcgactct
     2881 ctgagcacca aagatcagat caacaaaaac gctaagttcc tggaaagcag atacgtgtgt
     2941 gaactgcaga aggaacggct ggagaacgag ggccacgtgc ggggagttga aaacagattt
     3001 ctgactaagg acatcgtgcg agaggccaag aaaatcatcg acacccagat gcagtactat
     3061 ccagaaatcg acgagacatt caaggaaaag tacatcagcc tggtggaaac cagaagagag
     3121 tatttcgagg gtcctggcca gggcagccct ttcggctgga acggcgacct gaagaagtgg
     3181 tacgagatgc tgatgggcca ctgcacctac ttcccccagg agctccggtc cgtgaagtac
     3241 gcctacagcg ccgatctctt caacgccctg aacgacctga ataacctgat catccagaga
     3301 gataactctg agaagctgga ataccacgag aagtaccata tcatcgagaa cgtgttcaag
     3361 caaaagaaga agcccacact gaagcagatt gctaaagaaa tcggagtcaa ccctgaagat
     3421 atcaaaggct acaggatcac caagagcggc acccctgaat tcaccagctt caagctgttc
     3481 cacgacctga agaaggtcgt gaaggaccac gctatcctgg acgacattga cctgctgaat
     3541 cagatcgccg aaatcctgac catctatcag gataaggaca gcatcgtggc cgagctgggc
     3601 cagctggagt acctgatgag cgaagccgat aagcagagca tttctgagct gaccggttac
     3661 accggcaccc atagcctgtc tctgaagtgc atgaacatga tcattgacga gctgtggcac
     3721 agcagcatga atcagatgga agtgttcacc tacctgaaca tgagacctaa gaaatacgag
     3781 ctgaaaggct accaaagaat ccctacagac atgatcgatg acgccatcct gtccccagtg
     3841 gtgaagagaa catttatcca gtctatcaat gtgatcaaca aggtgatcga gaagtacggc
     3901 atacccgagg acatcatcat agagttggct cgggaaaaca actccgatga cagaaagaaa
     3961 tttatcaaca atcttcagaa gaagaacgag gcaacaagga agcgcatcaa cgagatcatc
     4021 ggccagaccg gaaaccagaa cgctaaaaga attgtcgaga agatcagact gcacgaccag
     4081 caggagggaa agtgcctgta cagcctggaa agcatccccc tggaggatct gctgaacaac
     4141 cctaaccact acgaggtgga ccacatcatc ccaagaagcg tgtcctttga caatagctac
     4201 cacaataagg tgctggtgaa gcaatctgaa aattctaaga agagcaacct aaccccttac
     4261 cagtatttta acagcggaaa aagcaagctg tcttacaacc agtttaagca gcacatcctg
     4321 aacctgtcca aaagccagga cagaatcagc aagaagaaga aggagtattt gctggaagag
     4381 cgggacatca acaagttcga ggtgcagaaa gaattcatca accggaacct cgtggatacc
     4441 agatacgcta caagagagct gaccaactac ctgaaggcct acttcagcgc caacaacatg
     4501 aacgtgaagg tgaaaaccat caacggctct tttacagatt acctgagaaa ggtgtggaag
     4561 tttaagaagg aaagaaatca cggctacaag caccacgccg aggacgctct gatcatcgcc
     4621 aacgccgatt tcctgttcaa agagaacaag aagctgaagg ccgtgaacag cgttctggag
     4681 aaacctgaga tcgagaccaa gcagctggac atccaagtgg acagcgagga caactacagc
     4741 gaaatgttca tcattcctaa acaggtgcag gacatcaaag atttcaggaa cttcaagtac
     4801 tctcaccggg tggacaagaa gcccaacaga cagctgatca acgacaccct gtacagcacc
     4861 agaaaaaagg acaactccac ctatatcgtg caaacaatca aggacatcta cgccaaggat
     4921 aataccacct tgaagaaaca gttcgacaag agccccgaga agttcctgat gtaccaacac
     4981 gatcctagaa ccttcgagaa gctggaagtg attatgaagc agtacgccaa cgaaaaaaat
     5041 cccctggcca agtaccatga ggaaaccggc gagtacctga ccaaatactc caaaaaaaac
     5101 aacggcccca tcgtcaagag cctgaagtac atcggcaaca agctgggcag ccacctggat
     5161 gtgacccacc agttcaagag cagcacaaag aagcttgtga aactgagtat caagaattac
     5221 agattcgacg tgtacctgac cgagaagggc tacaagttcg tgaccatcgc ctatctgaac
     5281 gtgttcaaaa aagataacta ctactacatc cctaaagata agtaccagga gctgaaggag
     5341 aagaagaaga tcaaggacac agaccagttc atcgcctctt tctacaagaa tgacctcatt
     5401 aagcttaatg gagatctgta caaaattatc ggcgtgaact ctgatgatag aaacatcatc
     5461 gagctggatt actacgacat caagtacaag gactactgcg agatcaacaa tatcaagggc
     5521 gaacctcgga ttaagaagac aatcggcaag aagacagaga gcatcgagaa gttcacaacc
     5581 gacgtgctgg gaaatctgta tctgcacagc acggaaaagg cccctcaact gatcttcaag
     5641 agaggcctgg gatcccccaa gaagaagagg aaagtctcga gcgattacaa agacgatgac
     5701 gataagggca gcggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag
     5761 aatcctggac cgatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
     5821 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     5881 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     5941 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     6001 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     6061 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     6121 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agaattcgat
     6181 atcaagctta tcggtaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6241 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6301 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6361 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6421 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6481 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6541 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6601 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6661 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6721 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6781 ccgcatcgct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6841 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     6901 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6961 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7021 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7081 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7141 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7201 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7261 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7321 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7381 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7441 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     7501 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     7561 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7621 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     7681 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7741 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7801 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7861 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     7921 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7981 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     8041 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     8101 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     8161 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     8221 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     8281 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     8341 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     8401 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     8461 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     8521 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8581 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8641 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8701 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8761 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8821 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8881 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8941 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9001 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9061 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     9121 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9181 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9241 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9301 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9361 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9421 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9481 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9541 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9601 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9661 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9721 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9781 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9841 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9901 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9961 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    10021 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    10081 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    10141 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    10201 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    10261 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
    10321 ggagctgcaa gctt
//
