LOCUS       sequence-441015-       10325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized SlugCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Seo SY, Min S, Lee S, Seo JH, Park J, Kim HK, Song M, Baek D, Cho 
            SR, Kim HH
  TITLE     Massively parallel evaluation and computational prediction of the 
            activities and specificities of 17 small Cas9s.
  JOURNAL   Nat Methods. 2023 Jul;20(7):999-1009. doi: 
            10.1038/s41592-023-01875-2. Epub 2023 May 15.
  PUBMED    37188955
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-01875-2"; journalName: "Nat Methods"; date: 
            "2023-07"; volume: "20"; issue: "7"; pages: "999-1009"
COMMENT     Sequence Label: pLenti6.3-SlugCas9-BlastR
FEATURES             Location/Qualifiers
     source          1..10325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1079..1312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1497..1541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1820..2123
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2124..2327
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2278..2298
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2324..2348
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2399..2417
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2425..2434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2437..2457
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5647..5667
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5674..5697
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5707..5763
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5764..6162
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6187..6775
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6240..6260)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6658..6669)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6846..7079
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7151..7272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7188..7207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7242..7261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7312..7447
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7468..7486)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7496..7513)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7496..7512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7505..7527)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7654..8109
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7741..7760)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7951..7972
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8135..8239
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8240..8308,8309..9100)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8458..8477)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9271..9859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9760..9779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10013..10030
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10147..10168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10183..10188,10189..10206,10207..10213)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10221..10237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10226..10248
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10245..10261
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10245..10261
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10280..10300
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10282..10300
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
      961 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1021 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1081 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1141 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1201 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1261 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1321 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1381 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1441 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1501 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1561 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1621 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1681 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     1741 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     1801 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1861 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1921 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1981 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2041 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2101 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2161 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2221 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2281 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     2341 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactta
     2401 atacgactca ctatagggag agccgccacc atggcaccga agaagaagcg taaagtcgga
     2461 atccacggag ttcctgcggc aaaccagaaa ttcatcctgg gcctggacat cggaattaca
     2521 agcgtcggct acggcctgat cgattatgaa accaagaata tcatcgacgc cggcgtgcgg
     2581 ctgttccctg aggccaacgt ggaaaacaac gagggcagaa gaagcaagcg gggctccaga
     2641 cgactgaaga gaagaagaat ccatagactg gaaagagtga aaaaactcct ggaggactac
     2701 aacctgctgg accagagcca gatcccacaa agcacaaacc cctacgccat cagagtgaag
     2761 ggcctgagcg aggctctgtc aaaagacgag ctggtgatcg cactgctcca catcgctaag
     2821 aggaggggaa tccacaagat cgacgtgatc gacagcaacg acgatgttgg aaacgagctg
     2881 tctaccaagg agcagctgaa caaaaacagc aagctgctca aggacaagtt cgtgtgccag
     2941 atccaactgg aaagaatgaa cgagggacag gtcagaggag agaaaaacag attcaaaacc
     3001 gccgacatta ttaaggaaat catccagctg ctgaacgtgc aaaagaactt tcaccagctc
     3061 gacgagaact tcatcaacaa gtacatcgaa ctagtggaga tgcggcggga gtattttgag
     3121 ggccctggca agggctctcc ttacggctgg gagggcgatc ctaaggcctg gtatgagaca
     3181 ctgatgggcc actgtaccta cttccctgac gaactgagaa gcgtgaagta tgcctactcc
     3241 gccgatctgt tcaacgccct gaatgacctg aacaacctgg tcattcaacg ggacggcctg
     3301 tctaaactgg aataccatga gaaataccac atcatcgaaa acgtgtttaa gcagaaaaag
     3361 aagccaacac tgaagcagat cgccaatgag atcaacgtga accccgaaga tattaagggc
     3421 tatagaatta ccaagtctgg caagcctcag ttcacagagt tcaagctgta ccatgacctg
     3481 aagagcgtgc tgtttgacca gtctatcctg gagaatgagg atgtgctgga tcagatcgcc
     3541 gagatcctta caatctacca ggacaaggat agcatcaaat ctaaactcac agagctggat
     3601 atcctgctga acgaagagga caaggaaaac atcgcccagc tgaccggcta caccggcact
     3661 cacagattaa gcctgaagtg catccgcctc gttctggaag aacagtggta cagcagcaga
     3721 aaccagatgg aaatctttac ccacctgaac atcaagccta agaagatcaa cctgaccgcc
     3781 gccaataaga tccccaaggc catgatcgat gagttcatcc ttagccctgt ggtgaagaga
     3841 accttcggcc aggccattaa cctgatcaac aagatcatcg aaaagtacgg agtgccagag
     3901 gacatcatca tcgagctggc cagagagaac aactctaaag ataagcagaa gtttatcaac
     3961 gagatgcaga agaagaacga aaacaccagg aagagaatca atgaaatcat aggaaaatac
     4021 ggcaaccaaa acgccaagcg gctggtggag aagatccggc tgcacgacga gcaggagggc
     4081 aagtgcctgt actctctgga atccatcccc ctggaggacc tgctgaacaa ccctaaccac
     4141 tacgaagtgg accacatcat ccctaggtct gtgtccttcg acaacagcta ccacaacaaa
     4201 gtgctggtga aacaaagcga gaacagcaaa aagtctaacc tgacacctta ccagtacttc
     4261 aacagcggca aaagcaagct gtcttataat cagttcaaac agcacatcct gaacctgagc
     4321 aaatcccagg acagaatcag caagaagaag aaggaatacc tgctggagga acgggacatc
     4381 aataaattcg aggtgcagaa agaattcatc aacagaaacc tggtggacac cagatacgct
     4441 acacgggagt taacaaacta cctgaaggcc tacttcagcg ctaacaatat gaacgtgaaa
     4501 gtgaagacca tcaacggcag ttttacagac tacctgcgga aggtgtggaa gttcaagaaa
     4561 gaaagaaacc acggctacaa gcaccacgcc gaagacgccc tgatcatcgc taatgccgac
     4621 ttcctgttca aggaaaacaa gaaactgaag gccgtgaaca gcgtgctcga gaagcccgag
     4681 atcgagagca agcagctgga tatccaggtt gacagcgagg ataactacag cgaaatgttc
     4741 attatcccta agcaggtgca ggacattaag gattttagaa atttcaagta cagccacaga
     4801 gtggacaaga agcctaatcg gcagctgatc aatgataccc tgtacagcac gcggaagaag
     4861 gacaactcca catacatagt tcagaccatc aaagatatct acgccaagga caataccacc
     4921 ctgaagaagc agtttgataa gagccctgaa aagttcctga tgtaccagca cgaccctagg
     4981 acattcgaga agctggaggt gatcatgaaa cagtacgcca acgaaaaaaa ccccctggct
     5041 aagtaccacg aggaaacagg cgagtacctg accaagtaca gcaagaagaa caatggcccc
     5101 atcgtgaaga gcctgaagta tatcggcaac aagctgggaa gccacctgga tgtgacccac
     5161 cagttcaagt caagcacaaa aaagctggtc aagctgagta tcaagccgta cagattcgac
     5221 gtgtacctga ccgacaaggg ctataagttc atcacaatta gctacctgga cgtgctgaag
     5281 aaagacaact actactatat ccccgaacag aagtacgaca agctgaagct gggaaaggct
     5341 atcgataaaa atgccaagtt catcgctagc ttctacaaga atgacctcat caagctggat
     5401 ggcgagatct acaaaatcat tggcgtgaat agcgacacca gaaacatgat cgagctggat
     5461 ctgcctgata tcagatacaa ggaatactgc gagctgaaca atatcaaggg agagcctcgg
     5521 atcaagaaga ccatcggcaa aaaagtgaac tctatcgaaa agttaaccac cgacgtgctg
     5581 ggcaacgttt tcaccaacac ccagtacacc aagccacaac ttctgttcaa gagaggcaat
     5641 ggatccccca agaagaagag gaaagtctcg agcgattaca aagacgatga cgataagggc
     5701 agcggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
     5761 ccgatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca
     5821 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac
     5881 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc
     5941 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc
     6001 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat
     6061 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg
     6121 attcgtgaat tgctgccctc tggttatgtg tgggagggct aagaattcga tatcaagctt
     6181 atcggtaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6241 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6301 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6361 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6421 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6481 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6541 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     6601 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6661 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6721 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcgc
     6781 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6841 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     6901 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6961 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7021 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7081 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     7141 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7201 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7261 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     7321 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7381 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7441 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     7501 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     7561 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     7621 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     7681 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7741 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7801 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7861 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7921 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7981 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     8041 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     8101 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8161 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8221 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8281 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8341 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8401 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8461 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8521 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8581 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8641 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8701 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8761 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8821 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8881 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8941 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     9001 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     9061 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9121 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9181 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9241 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9301 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9361 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9421 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9481 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9541 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9601 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9661 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9721 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9781 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9841 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9901 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9961 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    10021 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    10081 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    10141 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    10201 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    10261 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
    10321 agctt
//
