LOCUS       sequence-441017-       10346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized SauriCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Seo SY, Min S, Lee S, Seo JH, Park J, Kim HK, Song M, Baek D, Cho 
            SR, Kim HH
  TITLE     Massively parallel evaluation and computational prediction of the 
            activities and specificities of 17 small Cas9s.
  JOURNAL   Nat Methods. 2023 Jul;20(7):999-1009. doi: 
            10.1038/s41592-023-01875-2. Epub 2023 May 15.
  PUBMED    37188955
REFERENCE   2  (bases 1 to 10346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-01875-2"; journalName: "Nat Methods"; date: 
            "2023-07"; volume: "20"; issue: "7"; pages: "999-1009"
COMMENT     Sequence Label: pLenti6.3-SauriCas9-BlastR
FEATURES             Location/Qualifiers
     source          1..10346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1079..1312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1497..1541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1820..2123
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2124..2327
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2278..2298
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2324..2348
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2399..2417
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2425..2434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2437..2457
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5668..5688
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5695..5718
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5728..5784
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5785..6183
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6208..6796
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6261..6281)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6679..6690)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6867..7100
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7172..7293
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7209..7228)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7263..7282
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7333..7468
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7489..7507)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7517..7534)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7517..7533)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7526..7548)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7675..8130
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7762..7781)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7972..7993
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8156..8260
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8261..8329,8330..9121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8479..8498)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9292..9880
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9781..9800
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10034..10051
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10168..10189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10204..10209,10210..10227,10228..10234)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10242..10258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10247..10269
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10266..10282
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10266..10282
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10301..10321
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10303..10321
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
      961 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1021 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1081 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1141 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1201 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1261 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1321 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1381 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1441 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1501 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1561 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1621 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1681 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     1741 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     1801 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1861 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1921 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1981 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2041 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2101 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2161 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2221 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2281 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     2341 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactta
     2401 atacgactca ctatagggag agccgccacc atggcaccga agaagaagcg taaagtcgga
     2461 atccacggag ttcctgcggc acaggagaac cagcagaagc agaattacat cctgggcctg
     2521 gacatcggca tcacctctgt gggctatggc ctgatcgaca gcaagacccg ggaagtgatc
     2581 gatgcaggcg tgcgcctgtt cccagaggca gactctgaga acaatagcaa ccggagatcc
     2641 aagaggggcg ccaggcgcct gaagcggcgg agaatccacc gcctgaatcg ggtgaaggac
     2701 ctgctggccg attaccagat gatcgacctg aacaatgtgc caaagtccac cgatccctat
     2761 acaatcagag tgaagggcct gagggagccc ctgaccaagg aggagtttgc catcgccctg
     2821 ctgcacatcg caaagaggag gggcctgcac aacatctctg tgagcatggg cgacgaggag
     2881 caggataacg agctgtctac caagcagcag ctgcagaaga atgcccagca gctgcaggac
     2941 aagtacgtgt gcgagctgca gctggagcgg ctgacaaaca tcaataaggt gcgcggcgag
     3001 aagaaccggt tcaagaccga ggattttgtg aaggaggtga agcagctgtg cgagacacag
     3061 agacagtatc acaatatcga cgatcagttc atccagcagt atatcgacct ggtgtccacc
     3121 agaagggagt actttgaggg acctggaaac ggctctccct acggctggga cggcgatctg
     3181 ctgaagtggt atgagaagct gatgggcagg tgcacatact tccctgagga gctgcgcagc
     3241 gtgaagtatg cctactccgc cgacctgttt aacgccctga atgatctgaa caatctggtg
     3301 gtgacccgcg acgataatcc aaagctggag tactatgaga agtaccacat catcgagaac
     3361 gtgttcaagc agaagaagaa tcccacactg aagcagatcg ccaaggagat cggcgtgcag
     3421 gactatgaca tcagaggcta caggatcacc aagtccggca agccccagtt cacatctttt
     3481 aagctgtacc acgacctgaa gaacatcttt gagcaggcca agtatctgga ggacgtggag
     3541 atgctggatg agatcgccaa gatcctgacc atctaccagg acgagatcag catcaagaag
     3601 gccctggatc agctgcctga gctgctgacc gagagcgaga agtcccagat cgcccagctg
     3661 acaggctata ccggcacaca ccggctgagc ctgaagtgca tccacatcgt gatcgacgag
     3721 ctgtgggagt cccccgagaa ccagatggag attttcacca gactgaatct gaagcctaag
     3781 aaggtggaga tgtctgagat cgacagcatc cctaccacac tggtggatga gttcatcctg
     3841 agcccagtgg tgaagagggc ctttatccag agcatcaaag tgatcaacgc cgtgatcaat
     3901 aggttcggcc tgccagagga catcatcatc gagctggccc gcgagaagaa ctccaaggat
     3961 cgccggaagt ttatcaacaa gctgcagaag cagaatgagg ccacccgcaa gaagatcgag
     4021 cagctgctgg ccaagtatgg caacacaaat gccaagtaca tgatcgagaa gatcaagctg
     4081 cacgacatgc aggagggcaa gtgtctgtac tctctggagg ccatccccct ggaggatctg
     4141 ctgagcaacc ctacccacta tgaggtggac cacatcatcc caaggtccgt gtctttcgat
     4201 aatagcctga acaataaggt gctggtgaag cagtctgaga acagcaagaa gggcaatcgc
     4261 acaccctatc agtacctgag cagcaacgag tccaagatca gctacaatca gtttaagcag
     4321 cacatcctga acctgtccaa ggccaaggac cggatcagca agaagaagcg ggacatgctg
     4381 ctggaggagc gggacatcaa taagttcgag gtgcagaagg agtttatcaa cagaaatctg
     4441 gtggacaccc gctacgccac acgggagctg tccaacctgc tgaagaccta tttctctaca
     4501 cacgattacg ccgtgaaggt gaagaccatc aacggcggct tcacaaatca cctgcggaaa
     4561 gtgtgggact tcaagaagca cagaaaccac ggctacaagc accacgcaga ggacgcactg
     4621 gtcatcgcca atgccgattt cctgtttaag acccacaagg ccctgagaag gacagacaag
     4681 atcctggagc agcccggcct ggaggtgaac gacaccacag tgaaggtgga taccgaggag
     4741 aagtaccagg agctgtttga gacacctaag caggtgaaga atatcaagca gttccgcgac
     4801 tttaagtact ctcaccgggt ggataagaag ccaaacagac agctgatcaa tgacaccctg
     4861 tatagcacaa gggagatcga tggcgagacc tatgtggtgc agacactgaa ggacctgtac
     4921 gccaaggata acgagaaggt gaagaagctg ttcaccgagc gcccacagaa gatcctgatg
     4981 taccagcacg accccaagac ctttgagaag ctgatgacaa tcctgaacca gtatgccgag
     5041 gccaagaatc ccctggccgc ctactatgag gataagggcg agtatgtgac caagtacgcc
     5101 aagaagggca atggccctgc catccacaag atcaagtaca tcgacaagaa gctgggctcc
     5161 tacctggacg tgagcaacaa gtaccctgag acacagaata agctggtgaa gctgagcctg
     5221 aagtccttcc ggtttgacat ctataagtgc gagcagggct acaagatggt gtccatcggc
     5281 tacctggacg tgctgaagaa ggataactac tattacatcc caaaggacaa gtatgaggcc
     5341 gagaagcaga agaagaagat caaggagtct gatctgttcg tgggcagctt ttattacaat
     5401 gacctgatca tgtacgagga tgagctgttc agagtgatcg gcgtgaacag cgacatcaac
     5461 aatctggtgg agctgaatat ggtggacatc acctataagg atttttgtga ggtgaacaat
     5521 gtgaccggcg agaagcggat caagaagaca atcggcaaga gagtggtgct gatcgagaag
     5581 tataccacag acatcctggg caacctgtac aagacccccc tgcctaagaa gcctcagctg
     5641 atcttcaaga ggggcgagct gggatccccc aagaagaaga ggaaagtctc gagcgattac
     5701 aaagacgatg acgataaggg cagcggcgca acaaacttct ctctgctgaa acaagccgga
     5761 gatgtcgaag agaatcctgg accgatggcc aagcctttgt ctcaagaaga atccaccctc
     5821 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
     5881 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
     5941 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
     6001 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
     6061 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
     6121 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
     6181 taagaattcg atatcaagct tatcggtaat caacctctgg attacaaaat ttgtgaaaga
     6241 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6301 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6361 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6421 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6481 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6541 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6601 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6661 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6721 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6781 tgggccgcct ccccgcatcg ctttaagacc aatgacttac aaggcagctg tagatcttag
     6841 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6901 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6961 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7021 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7081 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7141 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7201 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7261 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7321 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7381 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7441 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     7501 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     7561 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     7621 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     7681 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7741 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7801 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7861 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7921 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7981 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     8041 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8101 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     8161 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8221 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8281 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8341 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8401 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8461 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8521 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8581 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8641 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8701 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8761 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8821 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8881 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8941 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9001 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9061 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9121 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9181 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9241 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9301 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9361 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9421 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9481 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9541 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9601 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9661 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     9721 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9781 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9841 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9901 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9961 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    10021 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    10081 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    10141 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    10201 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    10261 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
    10321 ggaacaaaag ctggagctgc aagctt
//
