LOCUS       sequence-441018-       10328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized Sa-SlugCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10328)
  AUTHORS   Seo SY, Min S, Lee S, Seo JH, Park J, Kim HK, Song M, Baek D, Cho 
            SR, Kim HH
  TITLE     Massively parallel evaluation and computational prediction of the 
            activities and specificities of 17 small Cas9s.
  JOURNAL   Nat Methods. 2023 Jul;20(7):999-1009. doi: 
            10.1038/s41592-023-01875-2. Epub 2023 May 15.
  PUBMED    37188955
REFERENCE   2  (bases 1 to 10328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-01875-2"; journalName: "Nat Methods"; date: 
            "2023-07"; volume: "20"; issue: "7"; pages: "999-1009"
COMMENT     Sequence Label: pLenti6.3-Sa-SlugCas9-BlastR
FEATURES             Location/Qualifiers
     source          1..10328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1079..1312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1497..1541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1820..2123
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2124..2327
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2278..2298
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2324..2348
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2399..2417
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2425..2434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2437..2457
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5650..5670
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5677..5700
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5710..5766
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5767..6165
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6190..6778
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6243..6263)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6661..6672)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6849..7082
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7154..7275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7191..7210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7245..7264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7315..7450
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7471..7489)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7499..7516)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7499..7515)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7508..7530)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7657..8112
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7744..7763)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7954..7975
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8138..8242
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8243..8311,8312..9103)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8461..8480)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9274..9862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9763..9782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10016..10033
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10150..10171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10186..10191,10192..10209,10210..10216)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10224..10240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10229..10251
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10248..10264
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10248..10264
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10283..10303
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10285..10303
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
      961 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1021 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1081 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1141 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1201 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1261 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1321 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1381 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1441 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1501 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1561 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1621 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1681 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     1741 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     1801 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1861 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1921 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1981 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2041 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2101 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2161 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2221 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2281 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     2341 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactta
     2401 atacgactca ctatagggag agccgccacc atggcaccga agaagaagcg taaagtcgga
     2461 atccacggag ttcctgcggc aaagcgcaat tacatcctgg gtctggatat tggaatcacg
     2521 agcgtgggct acggcatcat cgactacgag acacgggacg tgatcgacgc cggcgtgcgg
     2581 ctgttcaagg aggccaacgt ggaaaacaac gagggcagac ggtccaagag aggcgccaga
     2641 cggctgaagc ggcgtcgacg gcaccggata cagagagtga agaagctgct gtttgactac
     2701 aacctgctga ccgaccacag cgagcttagc ggcatcaacc cttacgaggc tcgggtgaag
     2761 ggactgtccc agaaattatc tgaagaagag ttcagcgccg ccctgcttca tctggccaag
     2821 agaagaggcg tgcacaacgt gaacgaagtg gaggaagaca ccggcaacga gctgagcaca
     2881 aaggaacaga tcagcagaaa cagcaaggct ctggaagaga aatatgtggc cgagctgcag
     2941 ctggaaagac tgaagaagga cggcgaggtc agaggcagca tcaacagatt caagacaagc
     3001 gactacgtga aagaggccaa gcaactcctg aaggtgcaaa aggcatacca ccagctggat
     3061 cagagcttca tcgacaccta catcgatctg ctggaaacca gaagaaccta ctacgagggc
     3121 cctggtgagg gcagcccctt cggctggaaa gacatcaagg agtggtacga gatgctgatg
     3181 ggccactgta cctatttccc cgaggaactt agatccgtga agtacgccta caatgccgat
     3241 ctgtacaacg ctctgaacga tctgaacaac ctcgtgatca caagggacga gaacgaaaag
     3301 ctagaatact acgagaagtt ccagatcatc gagaatgttt tcaaacagaa gaagaaacca
     3361 accctgaagc agatcgccaa ggaaatcctg gtcaacgagg aggacatcaa gggctaccgg
     3421 gtcaccagca ccggcaagcc tgagttcacc aatctgaagg tgtaccacga catcaaggat
     3481 atcaccgcca gaaaggagat tatcgagaac gccgaactgc tggaccagat cgccaagatc
     3541 ctgaccatct accagagctc tgaggacatc caggaagaac tgaccaacct gaatagcgag
     3601 ctgacccagg aggagatcga gcagatctcc aacctgaagg gctacaccgg cacccacaac
     3661 ctgtctctga aagccattaa cctgatcctg gacgagctgt ggcacacaaa cgacaaccag
     3721 atcgctattt tcaacagact gaaattggtg cctaagaagg tggacctgag ccagcagaag
     3781 gaaattccta ccaccctggt ggacgacttt atcctgtcac cagtggtgaa aagaagcttt
     3841 atccagtcta tcaaggtgat caacgccatc atcaagaagt acggactgcc taacgacatt
     3901 attatcgagc tggccagaga aaaaaactcc aaggacgccc agaaaatgat caacgagatg
     3961 cagaagagaa atcggcagac caacgagcgg attgaagaga tcatcagaac aaccggcaaa
     4021 gagaacgcca agtacctgat tgaaaagatc aagctgcacg acatgcagga gggcaagtgc
     4081 ctgtacagcc tggaagccat ccccctggaa gatctgctga acaatccttt caactacgag
     4141 gtggaccaca tcatccccag aagcgtgtcc ttcgataaca gctttaataa caaagttctt
     4201 gtcaagcaag aagaaaacag caagaagggc aatagaaccc catttcagta cctgagctcc
     4261 tctgacagca agatctctta tgaaaccttc aagaaacaca tcctgaatct ggctaagggg
     4321 aagggcagaa tcagcaagac caagaaggag tacctgctcg aggaaagaga tatcaatcgg
     4381 tttagcgtgc agaaggactt catcaatcgg aatctggtgg acacccgata cgccacaaga
     4441 ggactgatga acctgctgag atcttacttc agagtcaaca acctggacgt gaaggtgaag
     4501 tctataaacg gcggcttcac cagcttcctg cgaagaaagt ggaagttcaa gaaggaacgg
     4561 aacaagggct ataaacacca cgccgaggat gccctgatca tcgctaatgc tgatttcatc
     4621 ttcaaagaat ggaagaagct tgacaaggca aagaaagtga tggagaacca gatgttcgag
     4681 gaaaagcagg ccgagagcat gcctgagatc gaaacagagc aagagtacaa agagatcttt
     4741 atcacgcccc accagatcaa gcatatcaag gatttcaagg attacaagta cagtcaccgg
     4801 gtggacaaga agccaaaccg ggaactgatc aacgacaccc tgtacagcac cagaaaggac
     4861 gacaaaggaa ataccctgat cgtgaacaac ctgaatggcc tgtatgacaa agacaacgac
     4921 aaactgaaga aactgatcaa caaaagccct gagaagctgc tgatgtacca tcacgatcct
     4981 cagacatacc agaagctgaa gttgattatg gaacaatacg gagatgagaa gaaccccctg
     5041 tataagtact atgaggagac aggcaactac ctgacaaagt acagcaagaa ggacaacgga
     5101 cctgtgatca agaagataaa gtactacggc aacaagctga acgcccacct ggacatcacc
     5161 gatgattacc ctaacagcag aaacaaggtg gtgaagctga gcctgaaacc ctacagattt
     5221 gatgtgtacc tgacagataa gggatacaag ttcatcacta tcagctacct ggacgtgctg
     5281 aaaaaagaca actactacta catccccgaa cagaagtacg acaagctgaa gctgggcaag
     5341 gccatcgaca agaacgctaa attcatcgcc agtttctaca agaatgatct gatcaaattg
     5401 gacggcgaaa tctacaagat catcggcgtg aactcagaca ccagaaatat gatcgagctg
     5461 gacctgcctg atatcagata caaggagtac tgcgagctga acaacatcaa aggcgagcct
     5521 cggatcaaaa agaccatcgg caagaaagtg aacagcatcg agaagctgac cacagacgtg
     5581 ctgggaaacg tgtttaccaa cacacagtac acaaagccgc aactgctgtt caagagaggc
     5641 aacggatccc ccaagaagaa gaggaaagtc tcgagcgatt acaaagacga tgacgataag
     5701 ggcagcggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga agagaatcct
     5761 ggaccgatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     5821 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     5881 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     5941 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     6001 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     6061 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     6121 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagaatt cgatatcaag
     6181 cttatcggta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6241 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6301 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6361 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6421 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6481 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6541 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6601 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6661 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6721 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     6781 cgctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6841 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     6901 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6961 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7021 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7081 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7141 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7201 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7261 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     7321 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7381 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7441 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     7501 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     7561 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     7621 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     7681 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7741 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7801 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7861 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7921 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7981 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     8041 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     8101 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     8161 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     8221 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     8281 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     8341 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     8401 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     8461 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     8521 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8581 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8641 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8701 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8761 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8821 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8881 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8941 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     9001 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     9061 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     9121 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     9181 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     9241 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     9301 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     9361 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     9421 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     9481 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9541 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9601 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9661 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9721 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9781 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9841 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9901 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9961 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    10021 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
    10081 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    10141 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    10201 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    10261 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
    10321 gcaagctt
//
