LOCUS       sequence-441037-       13721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit (MoClo-position 2): 2x35Ss_Omega 
            enhancer_PapE-4LF2-N-SpCas9i-N_tOCS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13721)
  AUTHORS   Schreiber T, Prange A, Schafer P, Iwen T, Grutzner R, Marillonnet S,
            Lepage A, Javelle M, Paul W, Tissier A
  TITLE     Efficient scar-free knock-ins of several kilobases in plants by 
            engineered CRISPR/Cas endonucleases.
  JOURNAL   Mol Plant. 2024 Mar 22:S1674-2052(24)00086-8. doi: 
            10.1016/j.molp.2024.03.013.
  PUBMED    38520090
REFERENCE   2  (bases 1 to 13721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2024 Mar 22:S1674-2052(24)00086-8. doi: 10.1016/j.molp.2024.03.013."
COMMENT     Sequence Label: pAGT9054
FEATURES             Location/Qualifiers
     source          1..13721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        409..754
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     697..717
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    773..828
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2849..2869
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8602..8622
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8639..9346
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    9458..9482
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      9574..10205
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10292..10396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10397..10465,10466..11257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10615..10634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11428..12016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11917..11936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12352..13500
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13656..13680
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggaggtcaac atggtggagc acgacactct ggtctactcc aaaaatgtca aagatacagt
       61 ctcagaagat caaagggcta ttgagacttt tcaacaaagg ataatttcgg gaaacctcct
      121 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg
      181 ctcctacaaa tgccatcatt gcgataaagg aaaggctatc attcaagatc tctctgccga
      241 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagaggttcc
      301 aaccacgtct acaaagcaag tggattgatg tgataacatg gtggagcacg acactctggt
      361 ctactccaaa aatgtcaaag atacagtctc agaagatcaa agggctattg agacttttca
      421 acaaaggata atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
      481 cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
      541 ggctatcatt caagatctct ctgccgacag tggtcccaaa gatggacccc cacccacgag
      601 gagcatcgtg gaaaaagaag aggttccaac cacgtctaca aagcaagtgg attgatgtga
      661 catctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
      721 tatataagga agttcatttc atttggagag gacacgctac tgtattttta caacaattac
      781 caacaacaac aaacaacaaa caacattaca attactattt acaattacaa tgcagactac
      841 tacccctgtg gatcctccat cttctcggtc tgaaaacaag aggcctcctg ctcctgctgg
      901 tgatgaaggt gctggtcctg gtagaggtct tgttgatcct gctagacctc ctaagaggcc
      961 aaggcctgat tctcttcctc ttgctgctgt gtgtagacct gctactcctc catcacctgg
     1021 tagaccagaa actcctccta ctcctgatct gcctttgtct cctagaggaa cccatggtat
     1081 tgcagctcca gctggcgaac ctgaacctgg ttctcctagt ctgcttgaga actatgttcc
     1141 tccagctcct gatgctggtg acgctggttc tactccagag cctggttggt ctgctgttgc
     1201 tattccagat gctctgcctt ctcatgtgct ggctgagact tttgagaggc acctttgcgg
     1261 tcttttgagg ggtgttagaa ggcctttgga tgttgagcct cttagagcta ggctgggcta
     1321 ccttttctct cttgctactg ctcttgaaga ggctggcatg gtggatagag gtgttggtgg
     1381 tcatcttctg aggctgtcta gaagggctgc tgctgcagat cctagacctc ttatggcttt
     1441 tttcgaggcc gctactcaga accaggctga atctcaactt tggggtctgc ttagaagggg
     1501 tcttactacc gcttctaccc ttaagtgggg acctagagga ccttgcttta gccctagatg
     1561 gctgaagaac aacggtgatc ctaggctgga cttccagtca tctgctgtta tgttcggtag
     1621 gaccaatgag cctgctgcta gggctctttt gttcaggtat tgcgttggca gggctgatga
     1681 tagagatgct gaaggtgatg atgctggcag gcgttttgtt ttttgcgagc ctggtgatgc
     1741 tcctgcagct tctgttcatg cttgcggtgt tcttgtggat gctcacactg gtatggtggg
     1801 cgcttctctt gatattcttg tgtgccctag agataggcac ggctgcctta atcctgctcc
     1861 aggtactcct cttaggttct acgaggttaa gtgccgggct aagtacgctt tcgatcctgc
     1921 tgatgcaggc gatcctgttg ttgctgctca tagaaggctt gtggctaggc gttctccatc
     1981 tgatttcagg gctttcttgc ggtctattgc taggcctggt gtgagatact ttgctcctgg
     2041 aagagttcct ggtcctgagg aagctcttgt ttctgatcat gctgtgtggg ctgatgcaag
     2101 agccggtgat gagaagagaa ggtgctctgc tttggacagg gctcttgtgg gtcttaattc
     2161 tggtgtggct tccgatgtgc tgcttttcgg agatcctgat ccagagagaa ggaccgtttc
     2221 tcctttggct tggtcatctg gtgctcttgt gcacagggaa cctatcttcg ctaatcctag
     2281 gcacccgaac ttcaagcaga ttctggttca ggcttacgtg ctggcttctc attttcctga
     2341 atgccctctt catcctcacc tcgtgacttt cattggtaga cataggaccc ctgatgagga
     2401 aggactttct ctgagacttg aggacgctcc agcttctgct ccagcagctg ttagggctgc
     2461 agctggtgct tctattcttc cagatcaggc tgttcctgtg gctctgatta ttacccctgt
     2521 tagggttgac gctgccatct acgatcttat ccgtaggaac tctaggctcg ccttcgatga
     2581 gactcttgct agactttggg cttctagggc tcctgcttct gatcctgccg ctgctggcga
     2641 aacttcttcg ggtggaggtg gttctggtgg aggtggatca ggaggaggag gttcgggtgg
     2701 aggtggttct ggtggaggtg gatcaggagg aggaggatca ggtggaggtg gttctggtgg
     2761 aggtggatca ggaggaggag gttcaggtgg aggtggttct ggtggaggtg gatcaggagg
     2821 aggaggatca ggtatggctt ctagcccacc gaagaagaag cggaaggtca gctggaaaat
     2881 ggacaagaag tacagcattg gacttgatat tggtacgaac tcagttgggt gggccgttat
     2941 caccgatgaa tacaaggtac cttcgaagaa atttaaagtg ctgggcaaca cagataggca
     3001 cagcattaag aagaacttga tcggagctct gctctttgac tctggagaaa ccgcggaggc
     3061 gacaaggctt aaacgtactg cgaggagaag gtacactcgc aggaagaaca gaatctgtta
     3121 tctccaagag atctttagca acgagatggc gaaggtaagg atttttatga tatagtatgc
     3181 ttatgtattt tgtactgaaa gcatatcctg cttcattggg atattactga aagcatttaa
     3241 ctacatgtaa actcacttga tgatcaataa acttgatttt gcaggttgac gactcgttct
     3301 tccatcgcct cgaggaatcc ttcctggtag aggaagataa gaaacacgag cgtcacccca
     3361 tctttgggaa tattgttgac gaagtagcct atcatgaaaa gtatccgact atataccacc
     3421 ttcgcaagaa gctggtggac tcaaccgata aggcagacct tcggctcata tacctggctc
     3481 tcgcgcacat gataaagttt cgtggccatt tcttgatcga aggggacctc aacccggata
     3541 actccgatgt ggataaactg ttcattcagc tcgtccaaac ctacaatcag ctgttcgagg
     3601 agaaccccat caatgcatca ggtaacattc cttagttacc tttcttttct ttttccatca
     3661 taagtttata gattgtacat gctttgagat ttttctttgc aaacaatctc aggtgtcgac
     3721 gccaaggcaa tactgtctgc cagactttcg aagtccagac ggcttgagaa tctgatcgct
     3781 caattgccag gcgagaagaa gaacggcttg ttcgggaatc tgattgcact gtctctgggc
     3841 ctcaccccta acttcaaaag caactttgac ctcgccgagg acgcgaagct gcagctgtca
     3901 aaggatacat acgatgatga tctggacaat ctgctcgccc aaataggtaa tcttgaaatt
     3961 ggaactcttc ttttgttgtc taaacctatc aatttctttg cggaaattta tttgaagctg
     4021 tagagttaaa attgagtctt ttaaactttt gtaggtgatc agtatgccga cctgttcttg
     4081 gctgccaaga atctgtcaga cgctatcttg ctcagtgaca ttctgcgggt caacacggag
     4141 ataaccaaag cgccacttag cgcctccatg atcaagaggt acgacgagca tcaccaggat
     4201 ctgacccttc tgaaggcttt ggttcgccag caactccccg agaagtacaa ggagattttc
     4261 tttgaccaat cgaagaatgg ctacgcaggg tacattgatg gaggtaagtt gttacttatg
     4321 attgttttcc tctctgctac atgtattttg ttgttcattt ctgtaagata taagaattga
     4381 gttttcctct gatgatatta ttaggtgcaa gtcaggagga attctacaaa ttcatcaagc
     4441 ctattctgga aaagatggac ggtacagagg agctgctcgt taaattgaac cgcgaagatt
     4501 tgcttcggaa gcagcgtacc ttcgacaatg gcagcatacc gcaccagatc cacctcggtg
     4561 agctgcatgc tatcttgagg aggcaagagg acttctatcc gttcctgaaa gacaacagag
     4621 agaagattga aaagatcctc acgttccgca ttccctacta tgtaggttag tatcatatga
     4681 agaaatacct agtttcagtt gatgaatgct attttctgac ctcagttgtt ctcttttgag
     4741 aattatttct tttctaattt gcctgatttt tctattaatt cattaggtcc actcgcacgc
     4801 gggaactcgc ggtttgcgtg gatgacacgc aaatccgagg agactatcac gccttggaac
     4861 ttcgaagagg tcgtggacaa gggtgcgagt gcacagtcct tcatcgaaag gatgaccaac
     4921 ttcgataaga atctcccaaa tgagaaagtc ctgcccaagc atagtctcct gtacgaatac
     4981 ttcacggtct acaacgagct gacgaaggtg aaatatgtga cggaggggat gcgcaaaccg
     5041 gccttcctgt caggtaaatc ctggtccaca cttttacgat aaaaacacaa gattttaaac
     5101 tatgaactga tcaataatca ttcctaaaag accacacttt tgttttgttt ctaaagtaat
     5161 ttttactgtt ataacaggtg agcagaagaa ggccattgtc gatctcttgt tcaaaaccaa
     5221 tcggaaggtc actgtgaaac agcttaaaga ggactacttt aagaagatcg aatgctttga
     5281 ttctgtggaa atcagcggcg ttgaggatag gttcaatgcc tctcttggca cataccatga
     5341 cctgttgaaa atcatcaagg acaaggactt ccttgacaac gaggagaacg aggacatcct
     5401 cgaggacatc gtgctgactc tcacgctgtt tgaggacaga gaaatgatcg aggagcgcct
     5461 taagacttat gcgcatctgt tcgatgacaa ggtcatgaag cagttgaaga ggaggagata
     5521 tacaggtaag aggtcaaaag gtttccgcaa tgatccctct ttttttgttt ctctagtttc
     5581 aagaatttgg gtatatgact aacttctgag tgttccttga tgcatatttg tgatgagaca
     5641 aatgtttgtt ctatgtttta ggttggggaa ggctctccag gaagctcatc aacggcatcc
     5701 gcgacaagca atccggcaag actatactgg actttctcaa atccgacggt tttgcgaatc
     5761 ggaacttcat gcagcttatt cacgatgact cactgacctt caaagaagat atccagaagg
     5821 cccaagtgtc aggtcagggc gatagccttc acgaacacat agccaacctg gctggatcgc
     5881 cagctataaa gaagggcata ctgcagacag tgaaggttgt ggatgagctg gtgaaggtaa
     5941 gttctgcatt tggttatgct ccttgcattt taggtgttcg tcgcacttcc atttccatga
     6001 atagctaaga ttttttttct ctgcattcat tcttcttgcc tcagttctaa ctgtttgtgg
     6061 tatttttgtt ttaattattg ctacaggtca tgggccgcca taagccggag aacatcgtca
     6121 tcgagatggc gagggaaaac cagacgactc agaaagggca gaagaactca cgggagcgca
     6181 tgaagcggat agaggaaggc atcaaggagc ttgggagtca gattctgaaa gagcacccag
     6241 tcgaaaatac tcaactccag aacgagaagc tgtacctcta ttacctccag aatgggagag
     6301 atatgtacgt cgaccaagag ctcgacatta acagactctc cgactatgat gtggatcaca
     6361 ttgtccctca atctttcctg aaggacgata gtattgacaa caaggtaaag caactgtgtt
     6421 ttaatcaatt tcttgtcagg atatatggat tataacttaa tttttgagaa atctgtagta
     6481 tttggcgtga aatgagtttg ctttttggtt tctcccgtgt tataggtcct tacgcgctca
     6541 gacaagaacc gcggaaaatc cgacaatgta cccagcgagg aggttgtgaa gaagatgaag
     6601 aactattgga ggcagctttt gaatgctaag ctcataaccc aacggaaatt cgacaatctc
     6661 acgaaggcag aaaggggcgg actgtctgag ctcgacaaag ccggcttcat caagcgccag
     6721 ttggttgaaa ctcgtcagat tacgaaacat gtggcccaga tactcgattc gcgtatgaat
     6781 acgaagtatg atgagaatga caaacttatc agggaggtaa aggtaaagtt tccaactttc
     6841 ctttaccata tcaaactaaa gttcgaaact ttttatttga tcaacttcaa ggccacccga
     6901 tctttctatt cctgattaat ttgtgatgaa tccatattga cttttgatgg ttacgcaggt
     6961 gatcaccctc aagagcaaac tggttagtga cttccggaag gacttccagt tttacaaggt
     7021 tcgcgagatc aacaactacc atcatgccca tgacgcctac ctgaacgccg ttgttggcac
     7081 tgctctcatc aagaagtatc cgaaactgga gtctgagttt gtgtacgggg attacaaggt
     7141 gtacgacgtt aggaagatga tcgcgaagtc agaacaagag atcggcaagg ctaccgcgaa
     7201 atacttcttt tactcgaata tcatgaactt cttcaagaca gagatcactc tggcgaatgg
     7261 tgaaatccgg aagaggcctc tgatcgagac aaatggcgaa acaggtctgt ctttcctatt
     7321 tcatatgttt aatcctagga atttgatcaa ttgattgtat gtatgtcgat cccaagactt
     7381 tcttgttcac ttatatctta actctctctt tgctgtttct tgcaggtgag attgtctggg
     7441 ataagggcag ggattttgcg actgtgcgta aggttctcag catgccccaa gtcaacatag
     7501 tcaagaaaac ggaggttcaa accggtggtt tctccaagga gtccattctc cctaagcgca
     7561 actccgacaa actgattgcg aggaagaagg attgggatcc gaagaaatac ggaggctttg
     7621 atagccctac cgtggcatac agcgtactgg tagtggccaa ggtggagaag ggcaagagca
     7681 agaaactgaa aagcgtcaag gaactgcttg gaattaccat aatggaaagg tcctcgttcg
     7741 agaagaatcc gatcgacttc ctcgaggcta aaggtaaaat attggatgcc agacgatatt
     7801 ctttcttttg atttgtaact ttttcctgtc aaggtcgata aattttattt tttttggtaa
     7861 aaggtcgata attttttttt ggagccatta tgtaattttc ctaattaact gaaccaaaat
     7921 tatactttgc aggttacaaa gaggtgaaga aagacctcat tatcaaactg cccaagtatt
     7981 cgcttttcga attggaaaat ggcagaaaac gcatgctggc atctgccgga gaactgcaga
     8041 agggcaacga gctggcattg cccagtaagt acgtcaactt cctgtacttg gcctcacact
     8101 atgagaagct gaaggggtca ccagaggaca acgagcagaa gcagttgttt gtcgagcagc
     8161 acaagcacta tcttgatgag atcatagagc agatcagcga attttccaag cgggtcattc
     8221 ttgcagacgc taacctcgat aaggtaagga cttctcatga atattagtgg cagattagtg
     8281 ttgttaaagt ctttggttag ataatcgatg cctcctaatt gtccatgttt tactggtttt
     8341 ctacaattac aggtgctttc cgcgtacaac aagcacagag ataagccgat aagggaacaa
     8401 gcggaaaaca tcatccacct gttcacactg accaatctgg gagccccagc agcctttaag
     8461 tacttcgata ccactatcga cagaaagcgc tacacatcaa ccaaggaagt gttggacgct
     8521 acccttattc accaatctat tacagggctc tatgagacaa ggatagatct gtcgcagttg
     8581 ggtggtgact ctagggctga cccaaagaag aagcgtaaag tcggttcgtg agcttgtcct
     8641 gctttaatga gatatgcgag aagcctatga tcgcatgata tttgctttca attctgttgt
     8701 gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt ttcggttcat
     8761 tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct ccgttcaatt
     8821 tactgattgt accctactac ttatatgtac aatattaaaa tgaaaacaat atattgtgct
     8881 gaataggttt atagcgacat ctatgataga gcgccacaat aacaaacaat tgcgttttat
     8941 tattacaaat ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa gactgattac
     9001 ataaatctta ttcaaatttc aaaagtgccc caggggctag tatctacgac acaccgagcg
     9061 gcgaactaat aacgctcact gaagggaact ccggttcccc gccggcgcgc atgggtgaga
     9121 ttccttgaag ttgagtattg gccgtccgct ctaccgaaag ttacgggcac cattcaaccc
     9181 ggtccagcac ggcggccggg taaccgactt gctgccccga gaattatgca gcattttttt
     9241 ggtgtatgtg ggccccaaat gaagtgcagg tcaaaccttg acagtgacga caaatcgttg
     9301 ggcgggtcca gggcgaattt tgcgacaaca tgtcgaggct cagcaggacc gctaagcttg
     9361 aattcttgct tgtcttcaca gagtggggcc cactgcatcc accccagtac attaaaaacg
     9421 tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc
     9481 caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag
     9541 gcagcccatc agtcagatca ggatctcctt tgcgacgctc accgggctgg ttgccctcgc
     9601 cgctgggctg gcggccgtct atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg
     9661 tgcgagacac cgcggccgcc ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga
     9721 cagatgaggg gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga
     9781 tgaggggcag gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga
     9841 aaacgcctga ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc
     9901 cctgcggtat tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac
     9961 acttgagggg cagagtgctg acagatgagg ggcgcaccta ttgacatttg aggggctgtc
    10021 cacaggcaga aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc
    10081 tgtcttttaa cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc
    10141 cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc
    10201 ccgctaacgc gggcctccca tccccccagg ggctgcgccc ctcggccgcg aacggcctca
    10261 ccccaaaaat ggcagcgctg gccaattcgt gcgcggaacc cctatttgtt tatttttcta
    10321 aatacattca aatatgtatc cgctcatgag acaataacct tgataaatgc ttcaataata
    10381 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
    10441 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
    10501 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
    10561 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
    10621 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
    10681 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
    10741 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
    10801 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
    10861 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
    10921 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
    10981 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
    11041 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
    11101 cggtgagcgt ggttctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    11161 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    11221 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    11281 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    11341 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    11401 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    11461 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    11521 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    11581 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    11641 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    11701 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    11761 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    11821 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    11881 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    11941 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    12001 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg cagatcctag
    12061 atgtggcgca acgatgccgg cgacaagcag gagcgcaccg acttcttccg catcaagtgt
    12121 tttggctctc aggccgaggc ccacggcaag tatttgggca aggggtcgct ggtattcgtg
    12181 cagggcaaga ttcggaatac caagtacgag aaggacggcc agacggtcta cgggaccgac
    12241 ttcattgccg ataaggtgga ttatctggac accaaggcac caggcgggtc aaatcaggaa
    12301 taagggcaca ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg
    12361 acgtttgacc ggaaggcata caggcaagaa ctgatcgacg cggggttttc cgccgaggat
    12421 gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc
    12481 ggctcgatgg tccagcaagc tacggccaag atcgagcgcg acagcgtgca actggctccc
    12541 cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc gtcgtcttga acaggaggcg
    12601 gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac caagaagcga
    12661 aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg
    12721 aaacacacga agcagcagat caaggaaatg cagctttcct tgttcgatat tgcgccgtgg
    12781 ccggacacga tgcgagcgat gccaaacgac acggcccgct ctgccctgtt caccacgcgc
    12841 aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt caacaaggac
    12901 gtgaagatca cctacaccgg cgtcgagctg cgggccgacg atgacgaact ggtgtggcag
    12961 caggtgttgg agtacgcgaa gcgcacccct atcggcgagc cgatcacctt cacgttctac
    13021 gagctttgcc aggacctggg ctggtcgatc aatggccggt attacacgaa ggccgaggaa
    13081 tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg
    13141 gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt
    13201 tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa
    13261 ttcatatggg agaagtaccg caagctgtcg ccgacggccc gacggatgtt cgactatttc
    13321 agctcgcacc gggagccgta cccgctcaag ctggaaacct tccgcctcat gtgcggatcg
    13381 gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga agagttgcga
    13441 ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc tggtgcattg caaacgctag
    13501 ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca gcgcctgatc tggggaaccc
    13561 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
    13621 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
    13681 aacactgata gtttaaacca cttcgtgcag aagacaatag t
//
