LOCUS       sequence-441105-        8743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector for expression of MEK1 S218D/S222D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8743)
  TITLE     Der Lab MEK1 Plasmids 2024
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MEK1-DD-V5
FEATURES             Location/Qualifiers
     source          1..8743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    136..369
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             554..598
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        829..1039
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        851..1047
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      898..1033
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1079..1681
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1724..1841
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1899..2399
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2318..2336
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3593..3634
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3659..4247
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3712..3732)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4130..4141)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4319..4552
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4630..4751
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4667..4686)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4721..4740
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4791..4926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4946..4965)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4947..4965)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4975..4992)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4975..4991)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4984..5006)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5133..5588
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5220..5239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5430..5451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5614..5718
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5719..5787,5788..6579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5937..5956)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6750..7338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7239..7258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7492..7509
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7626..7647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7662..7667,7668..7685,7686..7692)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7700..7716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7705..7727
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7724..7740
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7724..7740
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7759..7779
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7761..7779
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7807..8033
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8034..8214
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8261..8386
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
       61 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
      121 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
      181 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
      241 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
      301 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
      361 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
      421 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
      481 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
      541 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
      601 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
      661 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
      721 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg
      781 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
      841 atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
      901 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
      961 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     1021 ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta
     1681 acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     1741 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     1801 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     1861 gcagagatcc actttggcgc cggctcgagg cctgcaggtt gcgccttttc caaggcagcc
     1921 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     1981 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     2041 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     2101 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     2161 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     2221 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     2281 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     2341 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
     2401 ggctagcatg cccaagaaga agccgacgcc catccagctg aacccggccc ccgacggctc
     2461 tgcagttaac gggaccagct ctgcggagac caacttggag gccttgcaga agaagctgga
     2521 ggagctagag cttgatgagc agcagcgaaa gcgccttgag gcctttctta cccagaagca
     2581 gaaggtggga gaactgaagg atgacgactt tgagaagatc agtgagctgg gggctggcaa
     2641 tggcggtgtg gtgttcaagg tctcccacaa gccttctggc ctggtcatgg ccagaaagct
     2701 aattcatctg gagatcaaac ccgcaatccg gaaccagatc ataagggagc tgcaggttct
     2761 gcatgagtgc aactctccgt acatcgtggg cttctatggt gcgttctaca gcgatggcga
     2821 gatcagtatc tgcatggagc acatggatgg aggttctctg gatcaagtcc tgaagaaagc
     2881 tggaagaatt cctgaacaaa ttttaggaaa agttagcatt gctgtaataa aaggcctgac
     2941 atatctgagg gagaagcaca agatcatgca cagagatgtc aagccctcca acatcctagt
     3001 caactcccgt ggggagatca agctctgtga ctttggggtc agcgggcagc tcatcgacga
     3061 catggccaac gacttcgtgg gcacaaggtc ctacatgtcg ccagaaagac tccaggggac
     3121 tcattactct gtgcagtcag acatctggag catgggactg tctctggtag agatggcggt
     3181 tgggaggtat cccatccctc ctccagatgc caaggagctg gagctgatgt ttgggtgcca
     3241 ggtggaagga gatgcggctg agaccccacc caggccaagg acccccggga ggccccttag
     3301 ctcatacgga atggacagcc gacctcccat ggcaattttt gagttgttgg attacatagt
     3361 caacgagcct cctccaaaac tgcccagtgg agtgttcagt ctggaatttc aagattttgt
     3421 gaataaatgc ttaataaaaa accccgcaga gagagcagat ttgaagcaac tcatggttca
     3481 tgcttttatc aagagatctg atgctgagga agtggatttt gcaggttggc tctgctccac
     3541 catcggcctt aaccagccca gcacaccaac ccatgctgct ggcgtcaccg gtggtaagcc
     3601 tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgaccgcgtt aagtcgacaa
     3661 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3721 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3781 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3841 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3901 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3961 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4021 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4081 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4141 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4201 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     4261 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     4321 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     4381 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4441 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4501 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     4561 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     4621 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4681 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4741 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     4801 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4861 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4921 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     4981 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     5041 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     5101 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     5161 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5221 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5281 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5341 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5401 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5461 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5521 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     5581 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5641 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5701 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5761 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5821 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5881 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5941 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6001 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6061 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6121 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6181 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6241 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6301 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6361 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6421 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6481 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6541 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6601 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6661 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6721 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6781 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6841 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6901 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6961 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7021 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7081 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7141 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7201 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7261 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7321 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7381 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7441 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7501 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7561 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7621 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7681 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7741 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     7801 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     7861 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     7921 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     7981 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     8041 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8101 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8161 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     8221 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     8281 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     8341 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     8401 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     8461 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     8521 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     8581 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     8641 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     8701 aagtaagacc accgcacagc aagcggccgc tgatcttcag acc
//
