LOCUS       sequence-441118-        4409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into 
            pPD95.75 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Cao WX, Merritt DM, Pe K, Cesar M, Hobert O
  TITLE     Comparative analysis of new mScarlet-based red fluorescent tags in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Oct 7;228(2):iyae126. doi: 10.1093/genetics/iyae126.
  PUBMED    39103170
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-10-7"; volume: "228(2):iyae126. doi"; pages: " 
            10.1093/genetics/iyae126"
COMMENT     Sequence Label: pWXC078
FEATURES             Location/Qualifiers
     source          1..4409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(277..865)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(357..376)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1036..1827,1828..1896))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1659..1678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1897..2001)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2069..2087
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2165..2184)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2284..2306
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4284..4300)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4284..4300)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4297..4319)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4308..4324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4332..4338,4339..4356,4357..4362))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4377..4398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
       61 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      121 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      181 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      241 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      301 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      361 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      421 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      481 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      541 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      601 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      661 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      721 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      781 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
      841 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
      901 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
      961 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1021 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1081 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1141 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     1201 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1261 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1321 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     1381 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     1441 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     1501 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     1561 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     1621 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     1681 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     1741 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     1801 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     1861 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     1921 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     1981 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     2041 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct taaggccgcc
     2101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     2161 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     2221 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     2281 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     2341 agggcccgta cggccgacta gtaggaaaca gttatgtttg gtatattggg aatgtattct
     2401 gtcatttaag gcaaaaaccc catagacact actccacttt caaattatca acttctactc
     2461 accttccact gagcctcaaa cccaaacctt cttccgatct ttcttgcatc gtgctcatca
     2521 atacttgtga ttttagcaag aaaaacgccg tcctcgaaat cttgacaaac aataggggaa
     2581 accccaaaaa gcaaaagcag gaaaattaat ttcataaact ttcacttgat tgaaaaattc
     2641 caaaaatact ccaaaacttt ttcacgatga gcatgatttg acgtcatgag aggcccagac
     2701 gtgcgaagaa ataaaaattg cggtcataaa ctgaaacgta acatatgata aggtattttg
     2761 tgtgcggttt tttctatgat gtttcaaaaa aatttatcag aagtaaaaaa cataagaagt
     2821 gtacacagaa cattgtgtag attttgggat agaattatta ttaatggggg gagggagcac
     2881 aatttttttc gactttttat tacgaattaa ttctattttt gaatctacac aatttcattg
     2941 ttagaggtga cttaaaagaa gctaaaaaac aaagaaatta agagaagttt tttgataata
     3001 acaaaaatag ggggtgggag cacagggaga aagagcatgt agggatgttg aagagtaatt
     3061 ggacttagaa gtcagaggca cgggcgcgag atgctgaaaa tttaaatatt actcgttagt
     3121 tagtagaact cagtttaaac ttactctgat gacagcggcc cctattattt ttgacaccag
     3181 acaagttggt aatggtagcg accggcgctc agttggaatt ctacgaatgt taggatcctc
     3241 cggatcctcc ggtggagtga cgggcgacgg aacgctcgta ttgctcgacg acggtgtagt
     3301 cctcgttgtg ggaggtgatg tcgagcttac ggtcgatgtt gaaggctcct ggcatttgga
     3361 ctggcttctt ggccttgtag gtggtcttga agtcggcgag gtaacgtcct ccgtccttga
     3421 gacggagggc catcttgatg tctccctgaa aatttaaata tgtatggtta gttagtaccg
     3481 aactgtttaa acttaccttg aggacgacgt cctctgggta gagacgctcg gtggaggcct
     3541 cccatcccat ggtacgcttt tgcatgactg gtccgtctgg tgggaagttt cctccacgga
     3601 gcttgacctt gtagatgagg gttccgtcct cgagggaggt gtcttgggtg acggagacgg
     3661 ttcctccgtc ctcgaagatc atgacacgct cccacttgaa tccctctggg aaggattgct
     3721 tccagtagtc tgggatgtcg gctgggtgct tgatgaaggc acgggatccg tacatgaatt
     3781 gtggggagag gatgtcccag gagaatggga gtggtcctcc ctgaaaattt aaataatcag
     3841 ggttagttag tatatatatg tttaaactta ccttggtgac cttgagcttg gcggtttggg
     3901 ttccctcgta tggacgtccc tctccctctc cctcgatctc gaactcgtgt ccgttcatgg
     3961 atccctccat gtggaccttg aaacgcatga actccttgat gacggcctcg gtggagtcca
     4021 ttttttctac cggtaccctc caagggtcct cctgaaaatg ttctatgtta tgttagtatc
     4081 attcgaaaca tacctttggg tcctttggcc aatcccgggg atcctctaga gtcgacctgc
     4141 aggcatgcaa gcttatttca tttccaagtt gttagcgtat ccatcgttgt gagtgatttc
     4201 agccattttt aagctctgaa aatttaaatg agaatagtta gttagtaaga tcatgtttaa
     4261 acttacagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4321 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     4381 gtgagctaac tcacattaat tgcgttgcg
//
