LOCUS       sequence-441119-        4409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans codon-optimized mScarlet3 with 3 introns cloned into 
            pPD95.75 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Cao WX, Merritt DM, Pe K, Cesar M, Hobert O
  TITLE     Comparative analysis of new mScarlet-based red fluorescent tags in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Oct 7;228(2):iyae126. doi: 10.1093/genetics/iyae126.
  PUBMED    39103170
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-10-7"; volume: "228(2):iyae126. doi"; pages: " 
            10.1093/genetics/iyae126"
COMMENT     Sequence Label: pWXC077
FEATURES             Location/Qualifiers
     source          1..4409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    14..35
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(50..55,56..73,74..80)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    88..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     93..115
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     112..128
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     112..128
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2106..2128)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2228..2247
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2325..2343)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2411..2515
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2516..2584,2585..3376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2734..2753)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3547..4135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4036..4055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4289..4306
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
       61 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
      121 gctatgacca tgattacgcc aagctgtaag tttaaacatg atcttactaa ctaactattc
      181 tcatttaaat tttcagagct taaaaatggc tgaaatcact cacaacgatg gatacgctaa
      241 caacttggaa atgaaataag cttgcatgcc tgcaggtcga ctctagagga tccccgggat
      301 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
      361 gaggaccctt ggagggtacc ggtagaaaaa atggactcca ccgaggccgt catcaaggag
      421 ttcatgcgtt tcaaggtcca catggaggga tccatgaacg gacacgagtt cgagatcgag
      481 ggagagggag agggacgtcc atacgaggga acccaaaccg ccaagctccg tgtcaccaag
      541 gtaagtttaa acatatatat actaactaac cctgattatt taaattttca gggaggacca
      601 ctcccattct cctgggacat cctctcccca caattcatgt acggatcccg tgccttcacc
      661 aagcacccag ccgacatccc agactactgg aagcaatcct tcccagaggg attcaagtgg
      721 gagcgtgtca tgaacttcga ggacggagga gccgtctccg tcgcccaaga cacctccctc
      781 gaggacggaa ccctcatcta caaggtcaag ctccgtggaa ccaacttccc accagacgga
      841 ccagtcatgc aaaagaagac catgggatgg gaggcctcca ccgagcgtct ctacccagag
      901 gacgtcgtcc tcaaggtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat
      961 tttcagggag acatcaagat ggccctccgt ctcaaggacg gaggacgtta cctcgccgac
     1021 ttcaagacca cctaccgtgc caagaagcca gtccaaatgc caggagcctt caacatcgac
     1081 cgtaagctcg acatcacctc ccacaacgag gactacaccg tcgtcgagca atacgagcgt
     1141 tccgtcgccc gtcactccac cggaggatcc ggaggatcct aacattcgta gaattccaac
     1201 tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag gggccgctgt
     1261 catcagagta agtttaaact gagttctact aactaacgag taatatttaa attttcagca
     1321 tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct
     1381 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt
     1441 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga
     1501 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc
     1561 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat
     1621 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     1681 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg
     1741 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga
     1801 aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg
     1861 acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa
     1921 gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag
     1981 tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa
     2041 ctgtttccta ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt
     2101 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     2161 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     2221 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     2281 cacagatgcg taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct
     2341 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     2401 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     2461 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     2521 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     2581 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     2641 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     2701 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     2761 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2821 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2881 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2941 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3001 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     3061 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     3121 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     3181 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     3241 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     3301 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3361 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3421 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3481 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3541 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3601 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3661 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3721 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3781 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3841 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3901 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3961 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4021 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4081 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4141 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4201 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4261 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     4321 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     4381 caggtttccc gactggaaag cgggcagtg
//
