LOCUS       sequence-441124-        8719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV-driven expression of dCas9 with puromycin resistance (Adapted 
            from plasmid #68416).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8719)
  TITLE     LncRNA CISTR-ACT regulates cell size in human and mouse
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CMV-dCas9_puroR
FEATURES             Location/Qualifiers
     source          1..8719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             334..354
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(409..428)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    453..501
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      complement(815..1403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(895..914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1574..2365,2366..2434))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2197..2216
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2435..2539)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2614..2633)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2767..3146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3147..3350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3375..3974
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3375..3394)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3871..3891
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    4000..4121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4037..4056)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4091..4110
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        4239..4618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4619..4822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4819..4843
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4931..4940
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4937..4963,4964..4966,4967..7450,7451..7456,7457..7522,
                     7523..7525,7526..8719,1..321)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 cacaaacact accttgatga gatcatcgag caaataagcg aattctccaa aagagtgatc
       61 ctcgccgacg ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc
      121 atcagggagc aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct
      181 gcagccttca agtacttcga caccaccata gacagaaagc ggtacacctc tacaaaggag
      241 gtcctggacg ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac
      301 ctctctcagc tcggtggaga cagcagggct gaccccaaga agaagaggaa ggtgtagaag
      361 aattcgatcc ctaccggtta gtaatgagtt taaacggggg aggctaactg aaacacggaa
      421 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
      481 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
      541 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
      601 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctc
      661 catagtcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      721 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      781 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      841 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      901 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      961 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1021 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1081 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1141 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1201 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1261 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1321 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1381 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1441 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1501 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1561 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1621 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1681 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1741 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1801 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1861 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1921 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1981 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2041 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2101 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     2161 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     2221 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2281 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2341 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2401 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2461 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2521 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     2581 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2641 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     2701 ttaagctaca acaaggcaag gcttgaccga caattgctag cagagggcct gaattctcga
     2761 ggcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     2821 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2881 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2941 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     3001 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     3061 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     3121 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     3181 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     3241 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     3301 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
     3361 ctaccggtgc caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     3421 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     3481 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     3541 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     3601 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     3661 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     3721 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg agtctcgccc gaccaccagg
     3781 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     3841 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     3901 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     3961 agcccggtgc ctgagaattc atcgataccg tcgagatcta acttgtttat tgcagcttat
     4021 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4081 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gaaattcgat
     4141 ccggtaccaa ggtcgggcag gaagctagcg catgaagaat ctgcttaggg ttaggcgttt
     4201 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     4261 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     4321 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     4381 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     4441 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     4501 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4561 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4621 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     4681 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     4741 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     4801 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
     4861 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagagga
     4921 tcgaaccctt gccaccatgg acaagaagta ctccattggg ctcgctatcg gcacaaacag
     4981 cgtcggctgg gccgtcatta cggacgagta caaggtgccg agcaaaaaat tcaaagttct
     5041 gggcaatacc gatcgccaca gcataaagaa gaacctcatt ggcgccctcc tgttcgactc
     5101 cggggagacg gccgaagcca cgcggctcaa aagaacagca cggcgcagat atacccgcag
     5161 aaagaatcgg atctgctacc tgcaggagat ctttagtaat gagatggcta aggtggatga
     5221 ctctttcttc cataggctgg aggagtcctt tttggtggag gaggataaaa agcacgagcg
     5281 ccacccaatc tttggcaata tcgtggacga ggtggcgtac catgaaaagt acccaaccat
     5341 atatcatctg aggaagaagc ttgtagacag tactgataag gctgacttgc ggttgatcta
     5401 tctcgcgctg gcgcatatga tcaaatttcg gggacacttc ctcatcgagg gggacctgaa
     5461 cccagacaac agcgatgtcg acaaactctt tatccaactg gttcagactt acaatcagct
     5521 tttcgaagag aacccgatca acgcatccgg agttgacgcc aaagcaatcc tgagcgctag
     5581 gctgtccaaa tcccggcggc tcgaaaacct catcgcacag ctccctgggg agaagaagaa
     5641 cggcctgttt ggtaatctta tcgccctgtc actcgggctg acccccaact ttaaatctaa
     5701 cttcgacctg gccgaagatg ccaagcttca actgagcaaa gacacctacg atgatgatct
     5761 cgacaatctg ctggcccaga tcggcgacca gtacgcagac ctttttttgg cggcaaagaa
     5821 cctgtcagac gccattctgc tgagtgatat tctgcgagtg aacacggaga tcaccaaagc
     5881 tccgctgagc gctagtatga tcaagcgcta tgatgagcac caccaagact tgactttgct
     5941 gaaggccctt gtcagacagc aactgcctga gaagtacaag gaaattttct tcgatcagtc
     6001 taaaaatggc tacgccggat acattgacgg cggagcaagc caggaggaat tttacaaatt
     6061 tattaagccc atcttggaaa aaatggacgg caccgaggag ctgctggtaa agcttaacag
     6121 agaagatctg ttgcgcaaac agcgcacttt cgacaatgga agcatccccc accagattca
     6181 cctgggcgaa ctgcacgcta tcctcaggcg gcaagaggat ttctacccct ttttgaaaga
     6241 taacagggaa aagattgaga aaatcctcac atttcggata ccctactatg taggccccct
     6301 cgcccgggga aattccagat tcgcgtggat gactcgcaaa tcagaagaga ccatcactcc
     6361 ctggaacttc gaggaagtcg tggataaggg ggcctctgcc cagtccttca tcgaaaggat
     6421 gactaacttt gataaaaatc tgcctaacga aaaggtgctt cctaaacact ctctgctgta
     6481 cgagtacttc acagtttata acgagctcac caaggtcaaa tacgtcacag aagggatgag
     6541 aaagccagca ttcctgtctg gagagcagaa gaaagctatc gtggacctcc tcttcaagac
     6601 gaaccggaaa gttaccgtga aacagctcaa agaagactat ttcaaaaaga ttgaatgttt
     6661 cgactctgtt gaaatcagcg gagtggagga tcgcttcaac gcatccctgg gaacgtatca
     6721 cgatctcctg aaaatcatta aagacaagga cttcctggac aatgaggaga acgaggacat
     6781 tcttgaggac attgtcctca cccttacgtt gtttgaagat agggagatga ttgaagaacg
     6841 cttgaaaact tacgctcatc tcttcgacga caaagtcatg aaacagctca agaggcgccg
     6901 atatacagga tgggggcggc tgtcaagaaa actgatcaat gggatccgag acaagcagag
     6961 tggaaagaca atcctggatt ttcttaagtc cgatggattt gccaaccgga acttcatgca
     7021 gttgatccat gatgactctc tcacctttaa ggaggacatc cagaaagcac aagtttctgg
     7081 ccagggggac agtcttcacg agcacatcgc taatcttgca ggtagcccag ctatcaaaaa
     7141 gggaatactg cagaccgtta aggtcgtgga tgaactcgtc aaagtaatgg gaaggcataa
     7201 gcccgagaat atcgttatcg agatggcccg agagaaccaa actacccaga agggacagaa
     7261 gaacagtagg gaaaggatga agaggattga agagggtata aaagaactgg ggtcccaaat
     7321 ccttaaggaa cacccagttg aaaacaccca gcttcagaat gagaagctct acctgtacta
     7381 cctgcagaac ggcagggaca tgtacgtgga tcaggaactg gacatcaatc ggctctccga
     7441 ctacgacgtg gctgctatcg tgccccagtc ttttctcaaa gatgattcta ttgataataa
     7501 agtgttgaca agatccgata aagctagagg gaagagtgat aacgtcccct cagaagaagt
     7561 tgtcaagaaa atgaaaaatt attggcggca gctgctgaac gccaaactga tcacacaacg
     7621 gaagttcgat aatctgacta aggctgaacg aggtggcctg tctgagttgg ataaagccgg
     7681 cttcatcaaa aggcagcttg ttgagacacg ccagatcacc aagcacgtgg cccaaattct
     7741 cgattcacgc atgaacacca agtacgatga aaatgacaaa ctgattcgag aggtgaaagt
     7801 tattactctg aagtctaagc tggtctcaga tttcagaaag gactttcagt tttataaggt
     7861 gagagagatc aacaattacc accatgcgca tgatgcctac ctgaatgcag tggtaggcac
     7921 tgcacttatc aaaaaatatc ccaagcttga atctgaattt gtttacggag actataaagt
     7981 gtacgatgtt aggaaaatga tcgcaaagtc tgagcaggaa ataggcaagg ccaccgctaa
     8041 gtacttcttt tacagcaata ttatgaattt tttcaagacc gagattacac tggccaatgg
     8101 agagattcgg aagcgaccac ttatcgaaac aaacggagaa acaggagaaa tcgtgtggga
     8161 caagggtagg gatttcgcga cagtccggaa ggtcctgtcc atgccgcagg tgaacatcgt
     8221 taaaaagacc gaagtacaga ccggaggctt ctccaaggaa agtatcctcc cgaaaaggaa
     8281 cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc aagaaatacg gcggattcga
     8341 ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa gtggagaaag ggaagtctaa
     8401 aaaactcaaa agcgtcaagg aactgctggg catcacaatc atggagcgat caagcttcga
     8461 aaaaaacccc atcgactttc tcgaggcgaa aggatataaa gaggtcaaaa aagacctcat
     8521 cattaagctt cccaagtact ctctctttga gcttgaaaac ggccggaaac gaatgctcgc
     8581 tagtgcgggc gagctgcaga aaggtaacga gctggcactg ccctctaaat acgttaattt
     8641 cttgtatctg gccagccact atgaaaagct caaagggtct cccgaagata atgagcagaa
     8701 gcagctgttc gtggaacaa
//
